Intravenous Grafts of Human Amniotic Fluid-Derived Stem Cells Reduce Behavioral Deficits in Experimental Ischemic Stroke.

Amniotic fluid has been investigated as new cell source for stem cells in the development of future cell-based transplantation. This study reports isolation of viable human amniotic fluid-derived stem cells, labeled with multimodal iron oxide nanoparticles, and its effect on focal cerebral ischemia-reperfusion injury in Wistar rats. Middle cerebral artery occlusion of 60 min followed by reperfusion for 1 h, 6 h, and 24 h was employed in the present study to produce ischemia and reperfusion-induced cerebral injury in rats. Tests were employed to assess the functional outcome of the sensorimotor center activity in the brain, through a set of modified neurological severity scores used to assess motor and exploratory capacity 24 h, 14, and 28 days after receiving cellular therapy via tail vein. In our animal model of stroke, transplanted cells migrated to the ischemic focus, infarct volume decreased, and motor deficits improved. Therefore, we concluded that these cells appear to have beneficial effects on the ischemic brain, possibly based on their ability to enhance endogenous repair mechanisms.

Intravenous grafts of human amniotic fluid-derived stem cells reduce behavioral deficits in experimental ischemic stroke

Amniotic fluid has been investigated as new cell source for stem cells in the development of future cell-based transplantation. This study reports isolation of viable human amniotic fluid-derived stem cells, labeled with multimodal iron oxide nanoparticles, and its effect on focal cerebral ischemia–reperfusion injury in Wistar rats. Middle cerebral artery occlusion of 60 min followed by reperfusion for 1 h, 6 h, and 24 h was employed in the present study to produce ischemia and reperfusion-induced cerebral injury in rats. Tests were employed to assess the functional outcome of the sensorimotor center activity in the brain, through a set of modified neurological severity scores used to assess motor and exploratory capacity 24 h, 14, and 28 days after receiving cellular therapy via tail vein. In our animal model of stroke, transplanted cells migrated to the ischemic focus, infarct volume decreased, and motor deficits improved. Therefore, we concluded that these cells appear to have beneficial effects on the ischemic brain, possibly based on their ability to enhance endogenous repair mechanisms.

Intravenous grafts of human amniotic fluid-derived stem cells reduce behavioral deficits in experimental ischemic stroke

Amniotic fluid has been investigated as new cell source for stem cells in the development of future cell-based transplantation. This study reports isolation of viable human amniotic fluid-derived stem cells, labeled with multimodal iron oxide nanoparticles, and its effect on focal cerebral ischemia–reperfusion injury in Wistar rats. Middle cerebral artery occlusion of 60 min followed by reperfusion for 1 h, 6 h, and 24 h was employed in the present study to produce ischemia and reperfusion-induced cerebral injury in rats. Tests were employed to assess the functional outcome of the sensorimotor center activity in the brain, through a set of modified neurological severity scores used to assess motor and exploratory capacity 24 h, 14, and 28 days after receiving cellular therapy via tail vein. In our animal model of stroke, transplanted cells migrated to the ischemic focus, infarct volume decreased, and motor deficits improved. Therefore, we concluded that these cells appear to have beneficial effects on the ischemic brain, possibly based on their ability to enhance endogenous repair mechanisms.

Dados normativos para o teste de fluência verbal categoria animais em nosso meio / Normative data for the animals category verbal fluency test in our environment

Objetivos: Avaliar o desempenho na fluencia verbal em nossa populacao e verificar a influencia da idade e escolaridade. Metodos: Foram entrevistados 336 indivÝduos sem queixas neurologicas ou psiquiatricas, atraves doMini-Exame do Estado Mental (MEM) e geracao de animais em um minuto. Para efeitocomparativo e para verificacao dos niveis de corte, foram examinados 65 individuos com quadro de perda cognitiva, acompanhados no ambulatorio de Neurologia do Comportamento da EPM. Resultados: Na populacao "normal" tivemos media de 13,84 animais por minuto. Para os grupos de escolaridade: 11,92, para analfabetos; 12,82, para individuos com ate 4 anos incompletos; 13,45, para os de 4 a 8 anos incompletos; 15,88 para os com 8 ou mais anos de escolaridade. Houve diferença significante entre eles (p=0,0001). Para os grupos etarios, tivemos medias de: 13,79, para aqueles com idade inferior a 65 anos; 13.92 para os com idade igual ou superior a 65 anos (sem diferença estatistica). Determinamos para esses grupos dois niveis de corte: 9 para individuos com ate 8 anos de escolaridade com sensibilidade de 75 por cento para analfabetos, 100 por cento para baixa escolaridade, 87 por cento para mÚdia escolaridade; e especificidade de 79 por cento para analfabetos, 84 por cento para baixa escolaridade, 88 por cento para media escolaridade. Para o grupo de alta escolaridade o escore de corte foi de 13, com sensibilidade de 86 por cento e especificidade de 67 por cento. ConclusÒo: Devemos utilizarniveis diferenciados de corte no teste de fluencia verbal, em nosso meio, considerando os efeitos da escolaridade sobre o desempenho neste teste.

Germinoma localizado nos núcleos da base e tálamo com invasäo do tronco cerebral: relato de caso / Germinoma localizated in basal ganglia and thalamus with brain stem invasion: case report

Descrevemos o caso de um paciente com diagnóstico de germinoma nos núcleos da base e tálamo, com invasao do tronco cerebral, ressaltando as características observadas nos exames de tomografia computadorizada e de ressonância magnética, os possíveis diagnósticos diferenciais, e a necessidade da comprovaçao anátomo-patológica, por tratar-se de localizaçao pouco frequente de um tumor com possibilidade de evoluçao favorável após tratamento com quimio e radioterapia.