RESUMO
Monoclonal antibodies (mAbs) against bovine lymphocyte cell surface antigens namely, MHC Class I, MHC class II (DP, DQ and DR), CD3, CD4, CD8, gamma delta TCR, WC1N1 and WC1N2, were tested for their reactivity on apparently normal buffalo mononuclear cells prepared from spleen, lymph nodes and peripheral blood. All the mAbs cross-reacted with the buffalo mononuclear cells. The mean (+/-SD) CD4:CD8 cell ratio in the peripheral blood of apparently normal buffaloes was 1.08+/-0.049 while in the spleen and lymph nodes it was 0.90+/-0.080 and 1.81+/-0.430, respectively. The lymphocyte subsets in the buffaloes positive for tuberculosis by the single intra dermal (SID) test was found to be altered; the CD4 cells were reduced while the CD8 and gamma delta cells were increased. The mean CD4:CD8 ratio in the SID positive buffaloes was 0.36+/-0.010.
Assuntos
Búfalos/fisiologia , Citometria de Fluxo/veterinária , Subpopulações de Linfócitos/fisiologia , Teste Tuberculínico/veterinária , Tuberculose/veterinária , Animais , Tuberculose/diagnósticoRESUMO
Five Newcastle disease virus (NDV) isolates from pigeons were characterized by biological and molecular methods. Four of the five isolates were found to be velogenic with high intracerebral pathogenicity indices (ICPI). The fusion protein cleavage site (FPCS) sequences of these isolates had multiple basic amino acids RRQKRF at positions 112-116 and a phenyl alanine at position 117 characteristic of velogenic isolates. Three of these velogenic isolates were phylogenetically related to mesogenic vaccine virus strain and the fourth one to a few exotic velogenic isolates. The lentogenic isolate obtained in this study was identical with the LaSota strain.
Assuntos
Columbidae/virologia , Doença de Newcastle/virologia , Vírus da Doença de Newcastle/genética , Vírus da Doença de Newcastle/patogenicidade , Sequência de Aminoácidos , Substituição de Aminoácidos , Animais , Índia , Dados de Sequência Molecular , Vírus da Doença de Newcastle/classificação , Vírus da Doença de Newcastle/isolamento & purificação , Filogenia , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , Proteínas Virais/químicaRESUMO
This report describes the isolation and molecular characterization of Newcastle disease virus isolated from an apparently normal guinea fowl (Numida melagridis). With a mean death time of 54 h and intracerebral pathogenicity index of 1.80, the isolate has been identified as velogenic by biological methods. Fusion protein cleavage site amino acid sequence analysis of the isolate indicated the presence of two pairs of basic amino acids at the C-terminus of the F2 region and phenylalanine at the N-terminus of the F1 region, confirming the velogenic nature of the isolate. Phylogenetic analysis of the isolate revealed that this isolate is genotypically related to other neurotropic velogenic isolates like Iowa/Salsbury, Texas GB, Kansas/Manhattan and mesogenic Michigan.