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1.
Mol Microbiol ; 121(3): 565-577, 2024 03.
Artigo em Inglês | MEDLINE | ID: mdl-38396332

RESUMO

Plasmodium sporozoites are the highly motile and invasive forms of the malaria parasite transmitted by mosquitoes. Sporozoites form within oocysts at the midgut wall of the mosquito, egress from oocysts and enter salivary glands prior to transmission. The GPI-anchored major surface protein, the circumsporozoite protein (CSP) is important for Plasmodium sporozoite formation, egress, migration and invasion. To visualize CSP, we previously generated full-length versions of CSP internally tagged with the green fluorescent protein, GFP. However, while these allowed for imaging of sporogony in oocysts, sporozoites failed to egress. Here, we explore different strategies to overcome this block in egress and obtain salivary gland resident sporozoites that express CSP-GFP. Replacing the N-terminal and repeat region with GFP did not allow sporozoite formation. Lowering expression of CSP-GFP at the endogenous locus allowed sporozoite formation but did not overcome egress block. Crossing of CSP-GFP expressing parasites that are blocked in egress with wild-type parasites yielded a small fraction of parasites that entered salivary glands and expressed various levels of CSP-GFP. Expressing CSP-GFP constructs from a silent chromosome region from promoters that are active only post salivary gland invasion yielded normal numbers of fluorescent salivary gland sporozoites, albeit with low levels of fluorescence. We also show that lowering CSP expression by 50% allowed egress from oocysts but not salivary gland entry. In conclusion, Plasmodium berghei parasites with normal CSP expression tolerate a certain level of CSP-GFP without disruption of oocyst egress and salivary gland invasion.


Assuntos
Anopheles , Esporozoítos , Animais , Esporozoítos/metabolismo , Proteínas de Protozoários/genética , Proteínas de Protozoários/metabolismo , Anopheles/parasitologia , Oocistos , Plasmodium berghei/genética , Plasmodium berghei/metabolismo
2.
An Acad Bras Cienc ; 96(1): e20230791, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38656058

RESUMO

Although control of Covid-19 has improved, the virus continues to cause infections, such as tuberculosis, that is still endemic in many countries, representing a scenario of coinfection. To compare Covid-19 clinical manifestations and outcomes between patients with active tuberculosis infection and matched controls. This is a matched case-control study based on data from the Brazilian Covid-19 Registry, in hospitalized patients aged 18 or over with laboratory confirmed Covid-19 from March 1, 2020, to March 31, 2022. Cases were patients with tuberculosis and controls were Covid-19 patients without tuberculosis. From 13,636 Covid-19, 36 also had active tuberculosis (0.0026%). Pulmonary fibrosis (5.6% vs 0.0%), illicit drug abuse (30.6% vs 3.0%), alcoholism (33.3% vs 11.9%) and smoking (50.0% vs 9.7%) were more common among patients with tuberculosis. They also had a higher frequency of nausea and vomiting (25.0% vs 10.4%). There were no significant differences in in-hospital mortality, mechanical ventilation, need for dialysis and ICU stay. Patients with TB infection presented a higher frequency of pulmonary fibrosis, abuse of illicit drugs, alcoholism, current smoking, symptoms of nausea and vomiting. The outcomes were similar between them.


Assuntos
COVID-19 , Coinfecção , Hospitalização , SARS-CoV-2 , Humanos , COVID-19/complicações , Masculino , Brasil/epidemiologia , Estudos de Casos e Controles , Feminino , Pessoa de Meia-Idade , Coinfecção/epidemiologia , Hospitalização/estatística & dados numéricos , Adulto , Sistema de Registros , Tuberculose/complicações , Tuberculose/epidemiologia , Mortalidade Hospitalar , Pandemias , Idoso , Pneumonia Viral/complicações , Pneumonia Viral/epidemiologia , Betacoronavirus , Infecções por Coronavirus/complicações , Infecções por Coronavirus/epidemiologia
3.
BMC Pregnancy Childbirth ; 23(1): 18, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36627576

RESUMO

BACKGROUND: The assessment of clinical prognosis of pregnant COVID-19 patients at hospital presentation is challenging, due to physiological adaptations during pregnancy. Our aim was to assess the performance of the ABC2-SPH score to predict in-hospital mortality and mechanical ventilation support in pregnant patients with COVID-19, to assess the frequency of adverse pregnancy outcomes, and characteristics of pregnant women who died. METHODS: This multicenter cohort included consecutive pregnant patients with COVID-19 admitted to the participating hospitals, from April/2020 to March/2022. Primary outcomes were in-hospital mortality and the composite outcome of mechanical ventilation support and in-hospital mortality. Secondary endpoints were pregnancy outcomes. The overall discrimination of the model was presented as the area under the receiver operating characteristic curve (AUROC). Overall performance was assessed using the Brier score. RESULTS: From 350 pregnant patients (median age 30 [interquartile range (25.2, 35.0)] years-old]), 11.1% had hypertensive disorders, 19.7% required mechanical ventilation support and 6.0% died. The AUROC for in-hospital mortality and for the composite outcome were 0.809 (95% IC: 0.641-0.944) and 0.704 (95% IC: 0.617-0.792), respectively, with good overall performance (Brier = 0.0384 and 0.1610, respectively). Calibration was good for the prediction of in-hospital mortality, but poor for the composite outcome. Women who died had a median age 4 years-old higher, higher frequency of hypertensive disorders (38.1% vs. 9.4%, p < 0.001) and obesity (28.6% vs. 10.6%, p = 0.025) than those who were discharged alive, and their newborns had lower birth weight (2000 vs. 2813, p = 0.001) and five-minute Apgar score (3.0 vs. 8.0, p < 0.001). CONCLUSIONS: The ABC2-SPH score had good overall performance for in-hospital mortality and the composite outcome mechanical ventilation and in-hospital mortality. Calibration was good for the prediction of in-hospital mortality, but it was poor for the composite outcome. Therefore, the score may be useful to predict in-hospital mortality in pregnant patients with COVID-19, in addition to clinical judgment. Newborns from women who died had lower birth weight and Apgar score than those who were discharged alive.


Assuntos
COVID-19 , Mortalidade Hospitalar , Respiração Artificial , Adulto , Feminino , Humanos , Recém-Nascido , Gravidez , Peso ao Nascer , Brasil/epidemiologia , COVID-19/mortalidade , COVID-19/terapia , Hipertensão Induzida pela Gravidez , Prognóstico , Estudos Retrospectivos
4.
FASEB J ; 34(1): 365-385, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31914616

RESUMO

Structural conversion of cellular prion protein (PrPC) into scrapie PrP (PrPSc) and subsequent aggregation are key events associated with the onset of transmissible spongiform encephalopathies (TSEs). Experimental evidence supports the role of nucleic acids (NAs) in assisting this conversion. Here, we asked whether PrP undergoes liquid-liquid phase separation (LLPS) and if this process is modulated by NAs. To this end, two 25-mer DNA aptamers, A1 and A2, were selected against the globular domain of recombinant murine PrP (rPrP90-231) using SELEX methodology. Multiparametric structural analysis of these aptamers revealed that A1 adopts a hairpin conformation. Aptamer binding caused partial unfolding of rPrP90-231 and modulated its ability to undergo LLPS and fibrillate. In fact, although free rPrP90-231 phase separated into large droplets, aptamer binding increased the number of droplets but noticeably reduced their size. Strikingly, a modified A1 aptamer that does not adopt a hairpin structure induced formation of amyloid fibrils on the surface of the droplets. We show here that PrP undergoes LLPS, and that the PrP interaction with NAs modulates phase separation and promotes PrP fibrillation in a NA structure and concentration-dependent manner. These results shed new light on the roles of NAs in PrP misfolding and TSEs.


Assuntos
Amiloide/metabolismo , Aptâmeros de Nucleotídeos/química , Aptâmeros de Nucleotídeos/metabolismo , Extração Líquido-Líquido/métodos , Doenças Priônicas/patologia , Proteínas Priônicas/química , Proteínas Priônicas/metabolismo , Animais , Camundongos , Conformação de Ácido Nucleico , Doenças Priônicas/metabolismo , Proteínas Priônicas/isolamento & purificação , Ligação Proteica , Conformação Proteica , Dobramento de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Técnica de Seleção de Aptâmeros
5.
Phys Chem Chem Phys ; 21(2): 736-743, 2019 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-30543327

RESUMO

The conducting polymer, poly(3,4-ethylenedioxythiophene)-polystyrenesulfonic acid (PEDOT:PSS), is certainly one of the most important substitute materials for indium tin oxide in organic devices. Its metallic conductivity and transmittance bring favorable perspectives for organic photovoltaic applications. Although graphene oxide (GO) is not a good conductor, it can form high-quality thin films and can be transparent, and additionally, GO is an inexpensive material and can be easily synthesized. This study investigated how the conductivity of a composite film of graphene oxide (GO) and different amounts of PEDOT:PSS can be modified. The effects of GO:PEDOT:PSS composites with several PEDOT:PSS proportions were analyzed in regards to the composite molecular structure and ordering, charge transfer dynamics (in the femtosecond range), electrical properties and morphology. For the best conductivity ratio GO found with 5% PEDOT:PSS, a solvent treatment was also performed, comparing the resistivity of the film when treated with dimethyl sulfoxide (DMSO) and with ethylene glycol.

6.
Chemistry ; 24(6): 1365-1372, 2018 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-29131930

RESUMO

A synergistic effect resulting from the interaction of small (2.4-3.1 nm) naked Pt nanoparticles (NPs) imprinted on N-doped carbon supports is evidenced by structural, electronic and electrochemical characterization. The size and distribution of the sputtered Pt NPs are found to be related to the nature of the support because Pt NPs are preferentially located at Ngraphitic sites. In addition, Rutherford backscattering shows that a deeper penetration of the Pt NPs is obtained in the N-doped carbon support with larger pore diameters. The ligand effect of the N-doped carbon supports is found to occur by electron donation from Npyrrolic and Ngraphitic sites to the Pt NPs and the electron acceptor behavior of the C=Npyridinic sites. The carbon matrix acquires a basic characteristic (electron-richer, metallic behavior) capable of interacting with metallic NPs akin to a bimetallic-like system. The imprinted Pt NPs are active catalysts for oxidation, although displaying poor catalytic activity for reduction reactions. The catalyst N-doped carbon supports play an important role in the overall catalytic process, rather than only acting as a simple active phase carrier.

7.
Cell Microbiol ; 18(3): 437-50, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26399761

RESUMO

Autophagy plays an important role in the defence against intracellular pathogens. However, some microorganisms can manipulate this host cell pathway to their advantage. In this study, we addressed the role of host cell autophagy during Plasmodium berghei liver infection. We show that vesicles containing the autophagic marker LC3 surround parasites from early time-points after invasion and throughout infection and colocalize with the parasitophorous vacuole membrane. Moreover, we show that the LC3-positive vesicles that surround Plasmodium parasites are amphisomes that converge from the endocytic and autophagic pathways, because they contain markers of both pathways. When the host autophagic pathway was inhibited by silencing several of its key regulators such as LC3, Beclin1, Vps34 or Atg5, we observed a reduction in parasite size. We also found that LC3 surrounds parasites in vivo and that parasite load is diminished in a mouse model deficient for autophagy. Together, these results show the importance of the host autophagic pathway for parasite development during the liver stage of Plasmodium infection.


Assuntos
Autofagia/fisiologia , Interações Hospedeiro-Parasita/fisiologia , Fígado/parasitologia , Malária/patologia , Plasmodium berghei/patogenicidade , Animais , Proteínas Reguladoras de Apoptose/metabolismo , Proteína Beclina-1 , Fígado/patologia , Malária/parasitologia , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Proteínas Associadas aos Microtúbulos/metabolismo
8.
J Pept Sci ; 23(6): 421-430, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28425152

RESUMO

Antimicrobial peptides are recognized candidates with pharmaceutical potential against epidemic emerging multi-drug resistant bacteria. In this study, we use nuclear magnetic resonance spectroscopy and molecular dynamics simulations to determine the unknown structure and evaluate the interaction with dodecylphosphatidylcholine (DPC) and sodium dodecylsulphate (SDS) micelles with three W6 -Hylin-a1 analogs antimicrobial peptides (HyAc, HyK, and HyD). The HyAc, HyK, and HyD bound to DPC micelles are all formed by a unique α-helix structure. Moreover, all peptides reach the DPC micelles' core, which thus suggests that the N-terminal modifications do not influence the interaction with zwiterionic surfaces. On the other hand, only HyAc and HyK peptides are able to penetrate the SDS micelle core while HyD remains always at its surface. The stability of the α-helical structure, after peptide-membrane interaction, can also be important to the second step of peptide insertion into the membrane hydrophobic core during permeabilization. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.


Assuntos
Peptídeos Catiônicos Antimicrobianos/química , Micelas , Simulação de Dinâmica Molecular , Ressonância Magnética Nuclear Biomolecular , Fosforilcolina/análogos & derivados , Dodecilsulfato de Sódio/química , Interações Hidrofóbicas e Hidrofílicas , Fosforilcolina/química
9.
Traffic ; 15(10): 1066-82, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24992508

RESUMO

Malaria parasites go through an obligatory liver stage before they infect erythrocytes and cause disease symptoms. In the host hepatocytes, the parasite is enclosed by a parasitophorous vacuole membrane (PVM). Here, we dissected the interaction between the Plasmodium parasite and the host cell late endocytic pathway and show that parasite growth is dependent on the phosphoinositide 5-kinase (PIKfyve) that converts phosphatidylinositol 3-phosphate [PI(3)P] into phosphatidylinositol 3,5-bisphosphate [PI(3,5)P2 ] in the endosomal system. We found that inhibition of PIKfyve by either pharmacological or non-pharmacological means causes a delay in parasite growth. Moreover, we show that the PI(3,5)P2 effector protein TRPML1 that is involved in late endocytic membrane fusion, is present in vesicles closely contacting the PVM and is necessary for parasite growth. Thus, our studies suggest that the parasite PVM is able to fuse with host late endocytic vesicles in a PI(3,5)P2 -dependent manner, allowing the exchange of material between the host and the parasite, which is essential for successful infection.


Assuntos
Fígado/metabolismo , Fosfatos de Fosfatidilinositol/metabolismo , Plasmodium berghei/patogenicidade , Animais , Linhagem Celular Tumoral , Endocitose , Fígado/parasitologia , Camundongos , Carga Parasitária , Fosfatidilinositol 3-Quinases/metabolismo , Inibidores de Fosfoinositídeo-3 Quinase , Plasmodium berghei/fisiologia , Transporte Proteico , Canais de Potencial de Receptor Transitório/metabolismo
10.
Antimicrob Agents Chemother ; 60(1): 580-91, 2016 01.
Artigo em Inglês | MEDLINE | ID: mdl-26574006

RESUMO

Attempts to isolate novel antimicrobial peptides from microbial sources have been on the rise recently, despite their low efficacy in therapeutic applications. Here, we report identification and characterization of a new efficient antimicrobial peptide from a bacterial strain designated A3 that exhibited highest identity with Paenibacillus ehimensis. Upon purification and subsequent molecular characterization of the antimicrobial peptide, referred to as penisin, we found the peptide to be a bacteriocin-like peptide. Consistent with these results, RAST analysis of the entire genome sequence revealed the presence of a lantibiotic gene cluster containing genes necessary for synthesis and maturation of a lantibiotic. While circular dichroism and one-dimension nuclear magnetic resonance experiments confirmed a random coil structure of the peptide, similar to other known lantibiotics, additional biochemical evidence suggests posttranslational modifications of the core peptide yield six thioether cross-links. The deduced amino acid sequence of the putative biosynthetic gene penA showed approximately 74% similarity with elgicin A and 50% similarity with the lantibiotic paenicidin A. Penisin effectively killed methicillin-resistant Staphylococcus aureus (MRSA) and did not exhibit hemolysis activity. Unlike other lantibiotics, it effectively inhibited the growth of Gram-negative bacteria. Furthermore, 80 mg/kg of body weight of penisin significantly reduced bacterial burden in a mouse thigh infection model and protected BALB/c mice in a bacteremia model entailing infection with Staphylococcus aureus MTCC 96, suggesting that it could be a promising new antimicrobial peptide.


Assuntos
Antibacterianos/química , Bacteriocinas/química , Genoma Bacteriano , Paenibacillus/genética , Processamento de Proteína Pós-Traducional , Infecções Estafilocócicas/tratamento farmacológico , Sequência de Aminoácidos , Animais , Antibacterianos/biossíntese , Antibacterianos/farmacologia , Bacteriocinas/biossíntese , Bacteriocinas/genética , Bacteriocinas/farmacologia , Expressão Gênica , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Staphylococcus aureus Resistente à Meticilina/crescimento & desenvolvimento , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Família Multigênica , Paenibacillus/química , Paenibacillus/metabolismo , Estrutura Secundária de Proteína , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/mortalidade , Infecções Estafilocócicas/patologia , Análise de Sobrevida , Resultado do Tratamento
11.
Traffic ; 13(10): 1351-63, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22780869

RESUMO

The obligate intracellular liver stage of the Plasmodium parasite represents a bottleneck in the parasite life cycle and remains a promising target for therapeutic intervention. During this stage, parasites undergo dramatic morphological changes and achieve one of the fastest replication rates among eukaryotic species. Nevertheless, relatively little is known about the parasite interactions with the host hepatocyte. Using immunofluorescence, live cell imaging and electron microscopy, we show that Plasmodium berghei parasites are surrounded by vesicles from the host late endocytic pathway. We found that these vesicles are acidic and contain the membrane markers Rab7a, CD63 and LAMP1. When host cell vesicle acidification was disrupted using ammonium chloride or Concanamycin A during the late liver stage of infection, parasite survival was not affected, but schizont size was significantly decreased. Furthermore, when the host cell endocytic pathway was loaded with BSA-gold, gold particles were found within the parasite cytoplasm, showing the transport of material from the host endocytic pathway toward the parasite interior. These observations reveal a novel Plasmodium-host interaction and suggest that vesicles from the host endolysosomal pathway could represent an important source of nutrients exploited by the fast-growing late liver stage parasites.


Assuntos
Endocitose , Hepatócitos/metabolismo , Hepatócitos/parasitologia , Interações Hospedeiro-Parasita , Plasmodium berghei/crescimento & desenvolvimento , Plasmodium berghei/metabolismo , Animais , Vesículas Citoplasmáticas/química , Feminino , Proteínas de Membrana Lisossomal/análise , Camundongos , Camundongos Endogâmicos C57BL , Esquizontes/crescimento & desenvolvimento , Tetraspanina 30/análise , Células Tumorais Cultivadas , Proteínas rab de Ligação ao GTP/análise , proteínas de unión al GTP Rab7
12.
ACS Appl Mater Interfaces ; 16(24): 31500-31512, 2024 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-38842224

RESUMO

The increasing global demand for food and agrarian development brings to light a dual issue concerning the use of substances that are crucial for increasing productivity yet can be harmful to human health and the environment when misused. Herein, we combine insights from high-level quantum simulations and experimental findings to elucidate the fundamental physicochemical mechanisms behind developing graphene-based nanomaterials for the adsorption of emerging contaminants, with a specific focus on pesticide glyphosate (GLY). We conducted a comprehensive theoretical and experimental investigation of graphene-based supports as promising candidates for detecting, sensing, capturing, and removing GLY applications. By combining ab initio molecular dynamics and density functional theory calculations, we explored several chemical environments encountered by GLY during its interaction with graphene-based substrates, including pristine and punctual defect regions. Our results unveiled distinct interaction behaviors: physisorption in pristine and doped graphene regions, chemisorption leading to molecular dissociation in vacancy-type defect regions, and complex transformations involving the capture of N and O atoms from impurity-adsorbed graphene, resulting in the formation of new GLY-derived compounds. The theoretical findings were substantiated by FTIR and Raman spectroscopy, which proposed a mechanism explaining GLY adsorption in graphene-based nanomaterials. The comprehensive evaluation of adsorption energies and associated properties provides valuable insights into the intricate nature of these interactions, shedding light on potential applications and guiding future experimental investigations of graphene-based nanofilters for water decontamination.

13.
Microorganisms ; 12(5)2024 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-38792739

RESUMO

Rickettsial pathogens are among the emerging and re-emerging vector-borne zoonoses of public health importance. Reports indicate human exposure to Rickettsial pathogens in Namibia through serological surveys, but there is a lack of data on infection rates in tick vectors, hindering the assessment of the relative risk to humans. Our study sought to screen Ixodid ticks collected from livestock for the presence of Rickettsia species in order to determine infection rates in ticks and to determine the Rickettsia species circulating in the country. We collected and pooled Hyalomma and Rhipicephalus ticks from two adjacent regions of Namibia (Khomas and Otjozondjupa) and observed an overall minimum Rickettsia infection rate of 8.6% (26/304), with an estimated overall pooled prevalence of 9.94% (95% CI: 6.5-14.3). There were no statistically significant differences in the estimated pooled prevalence between the two regions or tick genera. Based on the nucleotide sequence similarity and phylogenetic analysis of the outer membrane protein A (n = 9) and citrate synthase (n = 12) genes, BLAST analysis revealed similarity between Rickettsia africae (n = 2) and Rickettsia aeschlimannii (n = 11), with sequence identities ranging from 98.46 to 100%. Our initial study in Namibia indicates that both zoonotic R. africae and R. aeschlimannii are in circulation in the country, with R. aeschlimannii being the predominant species.

14.
Microorganisms ; 12(4)2024 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-38674782

RESUMO

This research examined the positivity ratio of Crimean-Congo haemorrhagic fever (CCHF) antibodies in cattle and sheep within Namibia's Omaheke region after a human disease outbreak in the same geographical area. A total of 200 samples (100 cattle and 100 sheep) were randomly collected from animals brought to two regional auction sites, and then tested using the ID Screen® CCHF Double Antigen Multi-Species Enzyme-Linked Immunosorbent Assay kit. Of the cattle samples, 36% tested positive, while 22% of the sheep samples were seropositive. The cattle had a significantly higher positivity ratio than sheep at the individual animal level (p = 0.0291). At the herd level, 62.5% of cattle herds and 45.5% of sheep flocks had at least one positive animal, but this difference was statistically insignificant (p = 0.2475). The fourteen cattle farms with at least one seropositive animal were dispersed across the Omaheke region. In contrast, the ten sheep farms with seropositive cases were predominantly situated in the southern half of the region. The study concluded that the CCHF is endemic in the Omaheke region and likely in most of Namibia, underscoring the importance of continued surveillance and preventive measures to mitigate the impact of CCHFV on animal health and potential spillover into human populations.

16.
Biomol NMR Assign ; 17(2): 239-242, 2023 12.
Artigo em Inglês | MEDLINE | ID: mdl-37589820

RESUMO

Molecular chaperones aid proteins to fold and assemble without modifying their final structure, requiring, in several folding processes, the interplay between members of the Hsp70 and Hsp40 families. Here, we report the NMR chemical shift assignments for 1 H, 15 N, and 13 C nuclei of the backbone and side chains of the J-domain of the class B Hsp40 from Saccharomyces cerevisiae, Sis1, complexed with the C-terminal EEVD motif of Hsp70. The data revealed information on the structure and backbone dynamics that add significantly to the understanding of the J-domain-Hsp70-EEVD mechanism of interaction.


Assuntos
Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae , Humanos , Saccharomyces cerevisiae/metabolismo , Proteínas de Choque Térmico/química , Proteínas de Choque Térmico/metabolismo , Proteínas de Saccharomyces cerevisiae/química , Sequência de Aminoácidos , Ligação Proteica , Ressonância Magnética Nuclear Biomolecular , Proteínas de Choque Térmico HSP70/química , Proteínas de Choque Térmico HSP70/metabolismo , Peptídeos/química
17.
Biomolecules ; 13(3)2023 03 02.
Artigo em Inglês | MEDLINE | ID: mdl-36979394

RESUMO

The Notch signaling ligand JAG1 is overexpressed in various aggressive tumors and is associated with poor clinical prognosis. Hence, therapies targeting oncogenic JAG1 hold great potential for the treatment of certain tumors. Here, we report the identification of specific anti-JAG1 single-chain variable fragments (scFvs), one of them endowing chimeric antigen receptor (CAR) T cells with cytotoxicity against JAG1-positive cells. Anti-JAG1 scFvs were identified from human phage display libraries, reformatted into full-length monoclonal antibodies (Abs), and produced in mammalian cells. The characterization of these Abs identified two specific anti-JAG1 Abs (J1.B5 and J1.F1) with nanomolar affinities. Cloning the respective scFv sequences in our second- and third-generation CAR backbones resulted in six anti-JAG1 CAR constructs, which were screened for JAG1-mediated T-cell activation in Jurkat T cells in coculture assays with JAG1-positive cell lines. Studies in primary T cells demonstrated that one CAR harboring the J1.B5 scFv significantly induced effective T-cell activation in the presence of JAG1-positive, but not in JAG1-knockout, cancer cells, and enabled specific killing of JAG1-positive cells. Thus, this new anti-JAG1 scFv represents a promising candidate for the development of cell therapies against JAG1-positive tumors.


Assuntos
Imunoterapia Adotiva , Anticorpos de Cadeia Única , Animais , Humanos , Imunoterapia Adotiva/métodos , Ligantes , Linhagem Celular Tumoral , Células Jurkat , Anticorpos de Cadeia Única/genética , Mamíferos/metabolismo , Proteína Jagged-1/genética , Proteína Jagged-1/metabolismo
18.
ARP Rheumatol ; 2023 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-37728133

RESUMO

OBJECTIVE: To compare the 2-year retention rate between a second tumor necrosis factor alpha inhibitor (TNFi) and secukinumab (SEK) or ustekinumab (UST), in Psoriatic Arthritis (PsA) patients with previous inadequate response to their first TNFi. METHODS: Prospective longitudinal cohort study with a follow-up period of 2 years using the Nationwide Portuguese Reuma.pt database. Patients with a clinical diagnosis of PsA who also fulfill the CASPAR classification criteria, with previous treatment failure to a first-line TNFi and having started a second biotechnological drug (TNFi, SEK or UST) were included. The Cycling group was defined as switching from a first TNFi to a second TNFi, and the Swapping group as switching from a first TNFi to SEK or UST. Sociodemographic data, disease characteristics, disease activity scores and physical function at baseline and after 6, 12 and 24 months were recorded. Cox-proportional hazards regression was used to compare retention rates between Cycling and Swapping groups. To obtain a predictor model of 2-year discontinuation, a multivariable Cox regression model was performed. RESULTS: In total, 439 patients were included, 58% were female, with a mean age (standard deviation) of 49 (12) years. Globally, 75.6% initiated a second TNFi (Cycling group), and 24.4% started SEK/UST (Swapping group). The retention rates after 6, 12 and 24 months were 72%/66%/59% in the Cycling group; and 77%/66%/59% in the Swapping group. There were no significant differences in retention rates between both strategies (HR: 1.06, 95% CI 0.72-1.16). After 2 years of follow-up, 34.4% of patients discontinued their second biologic, mainly due to inefficacy (72.8%), with no differences found between groups. Baseline treatment with glucocorticoids was the only predictor of discontinuation after 2 years of follow-up (HR:1.668, 95% CI 1.154-2.409). CONCLUSIONS: After failure of a first TNF inhibitor, Cycling and Swapping strategies result in similar retention rates suggesting that both are acceptable in the management of patients with psoriatic arthritis.

19.
Front Med (Lausanne) ; 10: 1130218, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37153097

RESUMO

Objectives: To assess the ABC2-SPH score in predicting COVID-19 in-hospital mortality, during intensive care unit (ICU) admission, and to compare its performance with other scores (SOFA, SAPS-3, NEWS2, 4C Mortality Score, SOARS, CURB-65, modified CHA2DS2-VASc, and a novel severity score). Materials and methods: Consecutive patients (≥ 18 years) with laboratory-confirmed COVID-19 admitted to ICUs of 25 hospitals, located in 17 Brazilian cities, from October 2020 to March 2022, were included. Overall performance of the scores was evaluated using the Brier score. ABC2-SPH was used as the reference score, and comparisons between ABC2-SPH and the other scores were performed by using the Bonferroni method of correction. The primary outcome was in-hospital mortality. Results: ABC2-SPH had an area under the curve of 0.716 (95% CI 0.693-0.738), significantly higher than CURB-65, SOFA, NEWS2, SOARS, and modified CHA2DS2-VASc scores. There was no statistically significant difference between ABC2-SPH and SAPS-3, 4C Mortality Score, and the novel severity score. Conclusion: ABC2-SPH was superior to other risk scores, but it still did not demonstrate an excellent predictive ability for mortality in critically ill COVID-19 patients. Our results indicate the need to develop a new score, for this subset of patients.

20.
Acta Crystallogr C ; 68(Pt 4): m94-6, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22476143

RESUMO

The absolute configuration of strictosidinic acid, (2S,3R,4S)-3-ethenyl-2-(ß-D-glucopyranosyloxy)-4-{[(1S)-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indol-1-yl]methyl}-3,4-dihydro-2H-pyran-5-carboxylate, was determined from its sodium chloride trihydrate, poly[[diaqua((2S,3R,4S)-3-ethenyl-2-(ß-D-glucopyranosyloxy)-4-{[(1S)-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indol-2-ium-1-yl]methyl}-3,4-dihydro-2H-pyran-5-carboxylate)sodium] chloride monohydrate], {[Na(C(26)H(32)N(2)O(9))(H(2)O)(2)]Cl·H(2)O}(n). The strictosidinic acid molecule participates in intermolecular hydrogen bonds of the O-H...O and O-H...Cl types. The solid-state conformation was observed as a zwitterion, based on a charged pyridine N atom and a carboxylate group, the latter mediating the packing through coordination with the sodium cation.


Assuntos
Carbolinas/química , Glicosídeos/química , Modelos Moleculares , Conformação Molecular
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