Growth performance, fatty-acid composition, lipid deposition and hepatic-lipid metabolism-related gene expression in juvenile pond loach Misgurnus anguillicaudatus fed diets with different dietary soybean oil levels.

A 10 week feeding trial was conducted to evaluate the effects of different dietary soybean oil (SO) levels on growth performance, fatty-acid composition and lipid deposition in viscera, histology and histochemistry of liver, intestine and hepatic-lipid metabolism-related gene expressions in pond loach Misgurnus anguillicaudatus juveniles. Misgurnus anguillicaudatus (mean ± s.d. mass 0·40 ± 0·01 g) were fed five experimental diets containing SO at different concentrations: 0, 20, 32, 56 and 100% SO and a diet containing 100% fish oil (100% FO). The mass gains and specific growth rates of M. anguillicaudatus fed 20% SO and 100% FO diets were significantly higher than those of the other groups (P < 0·05). The lipid content of viscera and the amount of cytoplasmic vacuolation in the liver increased with incremental dietary SO level. Meanwhile, increasing dietary SO levels up-regulated the messenger (m)RNA levels of lipogenic genes (such as Δ6fad, scd, pparγ, fas and srebp-1) and down-regulated the mRNA levels of the lipolytic genes (such as pparα, cpt1, atgl and hsl) in the liver. The percentage of 20:4n-6 significantly (P < 0·05) increased with increasing dietary SO level, which might be correlated with the up-regulation of the mRNA level of Δ6fad. The highest levels of dietary SO, however, had a negative effect on growth performance, lipid deposition of viscera and histology and histochemstry of liver and intestine. The increased lipid accumulation induced by incremental dietary SO level probably occurred through different strategies for lipid metabolism as a result of competition between lipolysis and lipogenesis and between export and import of lipids in this species.

[The importance of the adaptation period in medical rehabilitation in attaining definitive work capacity]. / Vaznost perioda adaptacije u medicinskoj rehabilitaciji za postizanje definitivne radne sposobnosti.

Medical rehabilitation may bring functional improvement up to a level permitted by damages of the locomotor system, especially by those of the joints. At the start of rehabilitation the restricted motions are initially very painful and provoke an intensive protective spasm. In the course of rehabilitation the range of motions becomes more extensive, motions are less and less painful, until finally the pain disappears. The final condition which is medically considered as definite is the contracture due to damages of the joints and periarticular tissues. The process of rehabilitation includes a period of worker's adjustment to the new conditions. The worker learns to use the damaged part of the body in a different manner by means of adaptation, substitution and other mechanisms of adjustment. That is a period of voluntary control of the damaged parts, and yet some certain tenderness or pain is present. Adaptation is supposed to develop new neural patterns, a new sequence and coordination of motions, and consequently new motion schemes. So voluntary use of the damaged body part is substituted by automatic and painless control. Definite evaluation of working capacity and residual working ability is possible only after the period of adaptation.