RESUMO
Using the unique capability of JET to monotonically change the amplitude of the magnetic field ripple, without modifying other relevant equilibrium conditions, the effect of the ripple on the angular rotation frequency of the plasma column was investigated under the conditions of no external momentum input. The ripple amplitude was varied from 0.08% to 1.5% in Ohmic and ion-cyclotron radio-frequency (ICRF) heated plasmas. In both cases the ripple causes counterrotation, indicating a strong torque due to nonambipolar transport of thermal ions and in the case of ICRF also fast ions.
RESUMO
The purpose of this study is to describe the electromyographic (EMG) power spectral evolution during a muscle strengthening training based on ultrasound stimulation applied to the right rectus femoris muscle simultaneously with its maximum voluntary isometric contraction (MVIC) in ten healthy subjects. From the surface EMG of rectus femoris the Zero Crossing (ZC) and integrated EMG (IEMG) were calculated. The Mean Power Frequency (MPF) and Median Frequency (MF) from the EMG power spectrum were obtained using a maximum entropy method. An Analysis of Variance (ANOVA) for repeated measures showed significant changes for IEMG and ZC throughout sessions and for MPF, MF and ZC between the pretest and posttest. Immediate increases in MPF, MF and ZC were observed (paired t-test, p < 0.05) after training and an increment of IEMG during sessions (one-way ANOVA, p = 0.01). The ultrasound pulsed stimulation applied simultaneously with a MVIC produced the development of the muscle force. The mechanism of action of this application could be the mechanical effect, compression and rarefaction, produced by the ultrasound stimulation during the muscle contraction process.
Assuntos
Eletromiografia , Contração Isométrica/fisiologia , Modalidades de Fisioterapia , Processamento de Sinais Assistido por Computador , Terapia por Ultrassom , Potenciais de Ação/fisiologia , Adulto , Feminino , Análise de Fourier , Humanos , Masculino , Músculo Esquelético/fisiologiaRESUMO
Operators in Nuclear Power Plants can receive high doses during refuelling operations. A training programme for simulating refuelling operations will be useful in reducing the doses received by workers as well as minimising operation time. With this goal in mind, a virtual reality application is developed within the framework of the CIPRES project. The application requires doses, both instantaneous and accumulated, to be displayed at all times during operator training. Therefore, it is necessary to set up a database containing dose rates at every point in the refuelling plant. This database is based on radiological protection surveillance data measured in the plant during refuelling operations. Some interpolation routines have been used to estimate doses through the refuelling plant. Different assumptions have been adopted in order to perform the interpolation and obtain consistent data. In this paper, the procedures developed to set up the dose database for the virtual reality application are presented and analysed.
Assuntos
Instrução por Computador/métodos , Bases de Dados Factuais , Exposição Ocupacional/análise , Centrais Elétricas/educação , Proteção Radiológica/métodos , Radiometria/métodos , Software , Interface Usuário-Computador , Simulação por Computador , Sistemas de Gerenciamento de Base de Dados , Ecossistema , Armazenamento e Recuperação da Informação/métodos , Capacitação em Serviço/métodos , Modelos Teóricos , Reatores Nucleares , Doses de Radiação , Medição de Risco/métodos , Fatores de RiscoRESUMO
BACKGROUND AND AIMS: The objective of this research was to characterize the physiology and cell ultrastructure of two soybean genotypes subjected to nutrient solutions with increasing concentrations of manganese (Mn) at two contrasting iron (Fe) concentrations. Genotypes 'PI227557' and 'Biloxi' were selected based on their distinctly different capacities to accumulate Mn and Fe. * METHODS: Bradyrhizobium-inoculated plants were grown in hydroponic cultures in a greenhouse. Nutrient solutions were supplied with Mn concentrations ranging from 0.3 to 90 microm, at either 5 or 150 microm Fe as FeEDTA. * KEY RESULTS: For both genotypes and at both Fe concentrations, Mn concentrations from 6.6 to 50 microm did not affect shoot, root and nodule mass, or leaf and nodule ureide concentration. Mn concentrations of 70 and 90 microm did not result in visible toxicity symptoms, but hindered growth and nodulation of 'Biloxi'. An Mn concentration of 0.3 microm was, however, deleterious to growth and nodulation for both genotypes, and caused an accumulation of ureides in leaves and major alterations in the ultrastructure of chloroplasts, nuclei and mitochondria, regardless of the Fe concentration. In 'PI227557', there was also a proliferation of Golgi apparatus and endoplasmic reticulum in the cytoplasm of leaf cells, and nodules showed disrupted symbiosomes lacking poly-beta-hydroxybutirate grains concomitantly with a proliferation of endoplasmic reticulum as well as arrested bacterial division. At 15 microm Fe, ferritin-like crystals were formed in the lumen of chloroplasts of 'PI227557' plants. For both genotypes, there was an antagonism between the Fe and Mn concentrations in leaves, the higher values of both microelements being detected in 'PI227557'. The absence of any detectable relationship between Fe or Mn and zinc, phosphorus and copper concentrations in leaves ruled out those micronutrients as relevant for Mn and Fe nutrition in soybeans. * CONCLUSIONS: The results confirmed the greater capacity of 'PI227557' for Mn and Fe accumulation than 'Biloxi' for most nutrient treatments. Hence, 'PI227557' may be a very useful genetic resource both in developing soybean cultivars for growth on low nutrient soils and in physiological studies to understand differing approaches to nutrient accumulation in plants.
Assuntos
Glycine max/genética , Glycine max/fisiologia , Ferro/fisiologia , Manganês/fisiologia , Bradyrhizobium , Clorofila/metabolismo , Genótipo , Hidroponia , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/ultraestrutura , Raízes de Plantas/crescimento & desenvolvimento , Brotos de Planta/crescimento & desenvolvimento , Glycine max/crescimento & desenvolvimento , Glycine max/ultraestruturaRESUMO
THREE TREATMENTS WHICH ALTERED TRANSLOCATION RATE WERE APPLIED TO CUCUMBER PLANTS: Girdling of source leaf petiole; removal of all aerial sinks; removal of all source leaves except one. Two different effects were observed, one short-term (during the initial 6 hours), and one long-term (detected after several days).The short-term effect was observed exclusively in girdled leaves and involved a reduction in (14)CO(2) fixation rate paralleled by an increase in stomatal resistance. The effects were maximal after 3 hours with subsequent recovery. Stomatal closure apparently resulted from the 5 to 10% water deficit temporarily detected in girdled leaves which probably induced the observed temporary increases in abscisic acid content. Kinetin counteracted the effects of girdling.The long-term effect was detected 3 days after girdling and 3 to 5 days after sink manipulation. An increase or decrease in (14)CO(2) fixation rate was observed when the sink-source ratio was increased or decreased respectively, accompanied by a respective decrease or increase in starch content. Changes in the relative amount of (14)CO(2) incorporated into various photosynthetic products were also observed. Stomatal closure was not involved, and the decrease in CO(2) fixation was not counteracted by kinetin.
RESUMO
When seven crop species were grown under identical environmental conditions, decreased sink:source ratio led to a decreased photosynthetic rate within 1 to 3 days in Cucumis sativus L., Gossypium hirsutum L., and Raphanus sativus L., but not in Capsicum annuum L., Solanum melongena L., Phaseolus vulgaris L., or Ricinus communis L. The decrease was not associated with stomatal closure. In cotton and cucumber, sink removal led to an increase in starch and sugar content, in glucose 6-phosphate and fructose 6-phosphate pools, and in the proportion of (14)C detected in sugar phosphates and UDPglucose following (14)CO(2) supply. When mannose was supplied to leaf discs to sequester cytoplasmic inorganic phosphate, promotion of starch synthesis, and inhibition of CO(2) fixation, were observed in control discs, but not in discs from treated plants. Phosphate buffer reduced starch synthesis in the latter, but not the former discs. The findings suggest that sink removal led to a decreased ratio inorganic phosphate:phosphorylated compounds. In beans (14)C in sugar phosphates increased following sink removal, but without sucrose accumulation, suggesting tighter feedback control of sugar level. Starch accumulated to higher levels than in the other plants, but CO(2) fixation rate was constant for several days.
RESUMO
Supercoiled filamentous virus particles with lengths of 400 to 700 nm and 3 nm wide were isolated from leaves of Carica papaya L. plants showing a mild yellowing between the veins. The morphological properties of this virus resemble those of tenuiviruses. However, it was serologically unrelated to three of the five definitive members of this group of plant viruses and had biochemical features quite different from tenuiviruses. Therefore, the virus described here is possible an unreported new virus infecting papaya for which the name of papaya mild yellow leaf virus (PMYLV) is proposed. PMYLV was mechanically transmitted to papaya and to several Cucurbitaceae species. Virus particles sedimented as one component in sucrose density gradients, containing one molecule of ssRNA with an apparent size of 6400 nucleotides which constitutes 5% of the particle weight. The buoyant density of PMYLV was 1.26 g/cm3 in cesium chloride equilibrium gradients, and the virus coat protein consisted of a single polypeptide with mol. wt. of c. 39 kDa. Estimated virus yield in purified preparations was 2.6 g/kg leaf tissues. An antiserum was produced with a titer of 1:1500. Ultrastructural observations of PMYLV-infected leaf tissues showed crystalline aggregates of virus particles, closely associated with electron dense amorphous inclusion bodies only within xylem cells.
Assuntos
Frutas/virologia , Vírus de Plantas/isolamento & purificação , Animais , Capsídeo/análise , Vírus de Plantas/química , Vírus de Plantas/ultraestrutura , CoelhosRESUMO
In a tokamak fusion reactor the energetic alpha particles will transiently stabilize the magnetohydrodynamic activity causing sawtooth oscillations. The crash events terminating long sawtooth free periods can provide seed islands for neoclassical tearing modes [Phys. Rev. Lett. 88, 105001 (2002)]]. To shorten the sawtooth periods localized current drive near the q=1 surface is a possibility. This Letter provides the first experimental evidence for the effectiveness of this method in the different physics regime associated with fast-ion-induced long sawteeth on the JET tokamak.
RESUMO
The onset of a neoclassical tearing mode (NTM) depends on the existence of a large enough seed island. It is shown in the Joint European Torus that NTMs can be readily destabilized by long-period sawteeth, such as obtained by sawtooth stabilization from ion-cyclotron heating or current drive. This has important implications for burning plasma scenarios, as alpha particles strongly stabilize the sawteeth. It is also shown that, by adding heating and current drive just outside the inversion radius, sawteeth are destabilized, resulting in shorter sawtooth periods and larger beta values being obtained without NTMs.
RESUMO
Third-harmonic ion-cyclotron-resonance heating of 4He-beam ions has produced for the first time on the JET tokamak high-energy populations of 4He ions to simulate 3.5 MeV fusion-born alpha (alpha) particles. Acceleration of 4He ions to the MeV energy range is confirmed by gamma-ray emission from the nuclear reaction 9Be(alpha,ngamma) 12C and excitation of Alfvén eigenmodes. Concomitant electron heating and sawtooth stabilization are observed. The scheme could be used in next-step tokamaks to gain information on trapped alpha particles and to test alpha diagnostics in the early nonactivated phase of operation.
RESUMO
Experiments on the JET tokamak show that the wave-induced pinch in the presence of toroidally asymmetric waves can provide a tool for controlling the profile of ion-cyclotron-resonant 3He ions. Direct evidence for the wave-induced pinch has been obtained from the measured gamma-ray emission profiles. Concurrent differences in the excitation of Alfvén eigenmodes (AEs), sawtooth stabilization, electron temperatures, and fast-ion stored energies are observed. The measured location of the AEs and gamma-ray emission profiles are consistent with the fast-ion radial gradient providing the drive for AEs.