1.
Methods Enzymol
; 145: 171-83, 1987.
Artigo
em Inglês
| MEDLINE
| ID: mdl-3298990
Assuntos
Colágeno , Fragmentos de Peptídeos/isolamento & purificação , Serina Endopeptidases , Animais , Bovinos , Brometo de Cianogênio , Eletroforese em Gel de Poliacrilamida/métodos , Endopeptidases , Matriz Extracelular/análise , Indicadores e Reagentes , Camundongos , Peso Molecular , Mapeamento de Peptídeos/métodos , Sarcoma Experimental/análise
2.
In Vitro
; 12(12): 845-6, 1976 Dec.
Artigo
em Inglês
| MEDLINE
| ID: mdl-799614
RESUMO
A small hot wire device for cutting plastic culture was can be constructed of steel rod, brass screws, nichrome wire and acrylic plastic sheeting and tubing. The nichrome wire is heated using a variable power transformer. Four sequential cuts are made in the culture flask bottom and the bottom separated from the remainder of the flask. Cultures can be stained, air-dried and cover slips affixed with PVP or epoxy resin. This method of cutting culture ware avoids the formation of small bits of polystyrene generated by rotating discs or saws.