RESUMO
Polyacrylamide-coated, carbon nanotube (PA/CNT) electrodes were prepared by an inkjet printing process and used to measure pyocyanin and uric acid in a wound fluid simulant at 37 °C. These two molecules are potential indicators of infection, and therefore their detection could prove useful for monitoring wound healing. Pyocyanin is a marker for the common wound bacterium Pseudomonas aeruginosa. Our long-term goal is to use these inexpensive and disposable electrodes to measure biomarkers of wound healing directly. In this proof-of-concept work, studies were performed in a wound fluid simulant to evaluate the stability of the electrodes and their responsiveness for the two bioanalytes. The PA/CNT inkjet-printed electrodes and electrical contacts were stable with unchanging physical and electrochemical properties in the wound fluid simulant over a 7-8-day period at 37 °C. The detection figures of merit for pyocyanin in the simulant at 37 °C were as follows: linear over the physiologically relevant range = 0.10 to 100 µmol L-1 (R2 = 0.9992), limit of detection = 0.10 µmol L-1 (S/N = 3), sensitivity = 35.6 ± 0.8 mA-L mol-1 and response variability ≤4% RSD. The detection figures of merit for uric acid in the simulant at 37 °C were as follows: linear over the physiologically relevant range = 100 to 1000 µmol L-1 (R2 = 0.9997), sensitivity = 2.83 ± 0.01 mA-L mol-1, and response variability ≤4% RSD. The limit of detection was not determined. The PA/CNT electrodes were also used to quantify pyocyanin concentrations in cell-free culture media from different strains of P. aeruginosa. The detected concentrations ranged from 1.00 ± 0.02 to 118 ± 6 µM depending on the strain.
Assuntos
Meios de Cultura/análise , Nanotubos de Carbono/química , Piocianina/análise , Ácido Úrico/análise , Ferimentos e Lesões/patologia , Técnicas Biossensoriais/instrumentação , Eletrodos , Desenho de Equipamento , Pseudomonas aeruginosa/isolamento & purificação , Ferimentos e Lesões/microbiologiaRESUMO
Homology threading is a powerful technology for generating structural models based on homologous structures. Here we use threading to generate four complex RNA polymerase models. The models appear to be as useful as x-ray crystal structures or cryo-electron microscopy structures to support research projects.
Assuntos
Biologia Computacional/métodos , RNA Polimerases Dirigidas por DNA/química , Modelos Moleculares , Conformação Proteica , Animais , Microscopia Crioeletrônica/métodos , Cristalografia por Raios X , RNA Polimerases Dirigidas por DNA/metabolismo , RNA Polimerases Dirigidas por DNA/ultraestrutura , Humanos , Conformação de Ácido Nucleico , Ácidos Nucleicos/química , Ácidos Nucleicos/metabolismo , Ácidos Nucleicos/ultraestruturaRESUMO
The human microbiome has been inextricably linked to multiple facets of human physiology. From an engineering standpoint, the ability to precisely control the composition and activity of the microbiome holds great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents. While the field of microbiome research is still in its infancy, growing engineering efforts are emerging to enable new studies in the microbiome and to rapidly translate these findings to microbiome-based interventions. At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
Assuntos
Bacteriófagos , Microbiota , Probióticos , Bacteriófagos/genética , Osso e Ossos/química , Humanos , Microbiota/genética , Prebióticos/análise , Probióticos/uso terapêuticoRESUMO
Collaborative research between educational institutions and health agencies is being increasingly used as a method of achieving joint outcomes and bringing together theory and practice. This paper reports on the experiences of collaboration that arose out of just such a project carried out in a residential aged care setting. The research team included university academics, two nurses in management positions in the aged care facility and a senior research assistant. In this paper, we explore some of the unexpected issues that emerged during implementation of the research project. The major challenges to successful collaboration arose not from within the collaborative research team, but from the responses of the broader staff who generally had little, if any, experience of research. Despite efforts to inform and involve staff, deep suspicions about the 'real' motives of the project proved difficult to shift. Trust and commitment are vital dimensions of successful collaborative research, yet gaining these from some staff proved elusive. Collaborative relationships between educational and practice settings need to be viewed as long-term endeavours driven by a common unifying goal to enhance client care. This has implications for costs and timelines which might be difficult to manage.