Bacterial community shifts evaluation in the sediments of Puyang River and its nitrogen removal capabilities exploration by resuscitation promoting factor.

Identifying indigenous bacterial community and exploring the potential of native microorganisms are crucial for in situ bioremediation of nitrogenous pollutants in water bodies. This study evaluated the bacterial communities of sediment samples from a nitrogen polluted river, and revealed the possible environmental factors shaping the bacterial populations. Importantly, viable but non-culturable bacteria which possessed nitrogen removal capabilities in indigenous population of the sediments were explored by resuscitation promoting factor (Rpf). It was found that the sediments from upstream (URS) and lower stream (LRS) of Puyang river showed both different pollutants levels and bacterial community. Nitrate nitrogen, organic carbon and ammonium nitrogen probably had a significant effect on bacterial compositions between URS and LRS. From URS and LRS, a total of thirteen strains with heterotrophic nitrification ability were resuscitated by Rpf addition, which belonged to genera Bacillus, Pseudomonas, Stenotrophomonas and Acinetobacter. Among them, the strain Pseudomonas sp. SSPR1 was found to display high removal capabilities of simultaneous nitrification and denitrification, and the average ammonium and nitrate removal rates were 2.23 and 0.86 mg/(L·h), respectively. These resuscitated strains could be considered to be used for biological nitrogen removal in rivers and their receiving water bodies.

A New Approach of Rpf Addition to Explore Bacterial Consortium for Enhanced Phenol Degradation Under High Salinity Conditions.

Only a small fraction of salt-tolerant phenol-degrading bacteria can be isolated by conventional plate separation methods, because most bacteria in nature are in a viable but non-culturable (VBNC) state. The aims of this study were to screen out more effective functional bacteria using resuscitation-promoting factor (Rpf), and to determine whether a mixed bacterial consortium possesses better phenol-degrading capabilities under high salinity conditions. The results indicated that three strains unique to treatment group with Rpf addition were obtained. A mixed bacterial consortium consisting of two high-efficient strains which belonged to genera Bacillus and Corynebacterium was capable of utilizing phenol as a sole source of carbon at high salinity. Complete degradation of 100 mg/L phenol at 2% NaCl concentration was achieved within 8 h. This study provides new insights into resuscitation of VBNC bacteria for enhanced treatment of phenol-laden saline wastewater.

Sustainable biodegradation of phenol by immobilized Bacillus sp. SAS19 with porous carbonaceous gels as carriers.

In this study, high-efficient phenol-degrading bacterium Bacillus sp. SAS19 which was isolated from activated sludge by resuscitation-promoting factor (Rpf) addition, were immobilized on porous carbonaceous gels (CGs) for phenol degradation. The phenol-degrading capabilities of free and immobilized Bacillus sp. SAS19 were evaluated under various initial phenol concentrations. The obtained results showed that phenol could be removed effectively by both free and immobilized Bacillus sp. SAS19. Furthermore, for degradation of phenol at high concentrations, long-term utilization and recycling were more readily achieved for immobilized bacteria as compared to free bacteria. Immobilized bacteria exhibited significant increase in phenol-degrading capabilities in the third cycle of recycling and reuse, which demonstrated 87.2% and 100% of phenol (1600 mg/L) degradation efficiency at 12 and 24 h, respectively. The present study revealed that immobilized Bacillus sp. SAS19 can be potentially used for enhanced treatment of synthetic phenol-laden wastewater.

Effect of Metal Ions on the Formation of Trichloronitromethane during Chlorination of Catechol and Nitrite.

Catechol, nitrite, and dissolved metals are ubiquitous in source drinking water. Catechol and nitrite have been identified as precursors for halonitromethanes (HNMs), but the effect of metal ions on HNM formation during chlorination remains unclear. The main objective of this study was to investigate the effect of metal ions (Fe, Ti, Al) on the formation of trichloronitromethane (TCNM) (the most representative HNM species in disinfected water) on chlorinating catechol and nitrite. Trichloronitromethane was extracted by methyl tert-butyl ether and detected by gas chromatography. The results show that metal ions promoted the formation of TCNM and that the enhancement efficiency followed the order of Fe > Ti > Al. Trichloronitromethane formation increased greatly within 2 h, and a basic condition (pH 8-9) favored TCNM formation more than acidic or neutral conditions. The conjoint effect of the metal-ion mixtures was shown to be similar to that of the single metal ion having the highest promoting effect on TCNM formation. Our results strongly suggest that metal ions play a significant role in enhancing TCNM formation.

Unveiling the inhibition of chlortetracycline photodegradation and the increase of toxicity when coexisting with silver nanoparticles.

Silver nanoparticles (AgNPs) and antibiotics inevitably co-exist in water environment. Nonetheless, little is known regarding the interactions between AgNPs and antibiotics or the effects of AgNPs on environmental behavior of antibiotics, particularly on sunlight-driven transformation. In the present work, we found that AgNPs obviously inhibit the photochemical decay of chlortetracycline (CTC), and CTC boosts the dissolution of AgNPs. With the help of electron paramagnetic resonance (EPR) and quenching experiment, we ascertained that these results originated from the competition between AgNPs against CTC for capturing 1O2 generated from CTC photosensitization. 1O2 reacting with CTC contributed mostly to CTC photodegradation, while 1O2 as well reacting with AgNPs leads to release of Ag+. When compared to reaction of 1O2 with CTC, 1O2 is prone to react with AgNPs, based on lower Gibbs free energy of AgNPs reacting with 1O2. Therefore, upon CTC co-existing with AgNPs, the release of Ag+ was accelerated and the photodegradation of CTC was inhibited obviously. Furthermore, the accelerated release of Ag+ significantly increased their toxicity toward E. coli cells under simulate sunlight irradiation. Overall, the findings demonstrate how AgNPs interact with CTC and how these interactions affect the environmental behaviors of CTC or AgNPs, allowing more accurate assessments of the risk to ecosystems posed by AgNPs coexisting with antibiotics.

Effect of Seed Sludge Type on Aerobic Granulation, Pollutant Removal and Microbial Community in a Sequencing Batch Reactor Treating Real Textile Wastewater.

The aerobic granulation, pollutant removal, and microbial community in real textile wastewater (TWW) treatment were compared using conventional activated sludge (CAS) and preformed aerobic granular sludge (AGS) in synthetic wastewater as seed in two reactors, reactor-1 (R1) and reactor-2 (R2), respectively. The results showed that complete granulation was achieved in R1 (sludge volume index at 5 min (SVI5) and 30 min (SVI30): 19.4 mL/g; granule size: 210 µm) within 65 days, while it only required 28 days in R2 (SVI5 and SVI30: 27.3 mL/g; granule size: 496 µm). The removal of COD, NH4+-N and TN in R1 (49.8%, 98.8%, and 41.6%) and R2 (53.6%, 96.9%, and 40.8%) were comparable in 100% real TWW treatment, but stable performance was achieved much faster in R2. The real TWW had an inhibitory effect on heterotrophic bacteria activity, but it had no inhibition on ammonia-oxidizing bacteria activity. AGS with a larger particle size had a higher microbial tolerance to real TWW. Furthermore, filamentous Thiothrix in the AGS in R2 disappeared when treating real TWW, leading to the improvement of sludge settleability. Thus, seeding preformed AGS is suggested as a rapid start-up method for a robust AGS system in treating real TWW.

Effective partial denitrification of biological effluent of landfill leachate for Anammox process: Start-up, influencing factors and stable operation.

Partial denitrification combined with Anammox is a promising approach for simultaneous removal of ammonium and nitrate from wastewaters. In this study, the start-up, influencing factors and stable operation of partial denitrification for treating biological effluent from landfill leachate were investigated. High nitrate loads (3.85 kg N m-3 d-1) and short hydraulic retention time (0.66 h) were obtained in the partial denitrification process, yielding a suitable ratio of NO2--N/NH4+-N in the effluent for downstream Anammox process. The study also revealed the importance of carbon sources, COD/NO3--N ratio and salinity in the partial denitrification. Acetate-type carbon source, COD/NO3--N ratio of about 3.0 and salinity lower than 1% favored high-efficient partial denitrification. The endogenous carbon sources from high-rate partial denitrification sludge contributed to low COD consumption in the process. During the partial denitrification, the dominant genus of Thauera was enriched, and shifted to Pseudomonas with the increase of organic removal rates.

Optimization of bacterial cytokine protein production by response surface methodology for environmental bioremediation.

In natural and engineered systems, most microorganisms would enter a state of dormancy termed as "viable but non-culturable" (VBNC) state when they are exposed to unpredictable environmental stress. One of the major advances in resuscitating from such a state is the discovery of a kind of bacterial cytokine protein called resuscitation-promoting factor (Rpf), which is secreted from Micrococcus luteus. In this study, the optimization of Rpf production was investigated by the response surface methodology (RSM). Results showed that an empirical quadratic model well predicted the Rpf yield, and the highest Rpf protein yield could be obtained at the optimal conditions of 59.56 mg L-1 IPTG, cell density 0.69, induction temperature 20.82 °C and culture time 7.72 h. Importantly, Phyre2 web portal characterized the structure of the Rpf domain to have a shared homology with lysozymes, and the highest lysozyme activity was at pH 5 and 50 °C. This study broadens the knowledge of Rpf production and provided potential strategies to apply Rpf as a bioactivator for environmental bioremediation.

Study on new artificial floating island removing pollutants.

In this study, two types of artificial floating islands (AFIs), group A (consists of 1# and 2# traditional AFIs with plant and soil) and group B (consists of 4# and 5# new-type AFIs with plant, substrate, and with luffa sponge and corncob hanging at the bottom), were constructed, respectively. The removal effects and degradation mechanisms of luffa sponge and corncob in group B were compared and investigated. Plant height, root growth, and packing degradation of the two types of AFIs were studied. Temperature, dissolved oxygen (DO), and pH on the decontamination effects of AFI were discussed. The results showed that group A and group B AFIs showed great significant differences in removal of CODCr, TN, NO3--N, NH4+-N, and TP (p < 0.05). The TP removal of group B was 92.8 ± 0.6%, and the TN removal and NO3--N removal were significantly higher than that of group A, which was 90.3 ± 0.8% and 96.0 ± 2.2%, respectively; The addition of luffa sponge and corncob could enhance the biodegradability of sewage and the nitrogen and phosphorus removal efficiency of group B. The plant growth height of group B planted with Lythrum salicaria was 2.36 times higher than that of group A. The effect of temperature on TP was significantly greater than that of TN, and both groups of AFIs presented continuous improvement capacities of TN and TP removal when the temperature was above 15 °C. Group B was observed with a lower pH range of 6.69~7.12, which was more suitable for denitrification than group A. The release of carbon source of 5#-corncob AFI was 2.51 times higher than 4#-luffa sponge AFI at the end of the experiment.

Characterization of a pH-Tolerant Strain Cobetia sp. SASS1 and Its Phenol Degradation Performance Under Salinity Condition.

Biological treatment of complex saline phenolic wastewater remains a great challenge due to the low activity of bacterial populations under stressful conditions. Acid mine drainage (AMD) as a typically extreme environment, shaped unique AMD microbial communities. Microorganisms survived in the AMD environment have evolved various mechanisms of resistance to low pH, high salinity and toxic heavy metals. The primary goal of this work was to determine whether a strain isolated from an AMD could degrade phenol under stressful conditions such as low pH, high salinity and heavy metals. The results suggested that the strain Cobetia sp. SASS1 isolated from AMD presented different physiological characteristics in comparison with five most closely related species. SASS1 can efficiently degrade phenol at wide ranges of pH (3.0-9.0) and NaCl concentration (0-40 g/L), as well as the existence of Cu2+ and Mn2+. Specifically, the SASS1 could completely degrade 1500 mg/L phenol in 80 h at 10 g/L NaCl. Meanwhile, mineralization of phenol was achieved with complete degradation of 900 mg/L phenol and simultaneously COD decreasing from 2239 mg/L to 181.6 mg/L in 36 h. Based on biodegradation metabolites identification and enzyme activities analysis, both ortho-cleavage pathway and benzoic acid pathway for phenol degradation were proposed. These findings suggested that SASS1 was an efficient phenol degrader under salinity and acidic conditions, and could be considered as key population for bioremediation of industrial phenolic wastewaters under stressful conditions.

Whole-genome sequencing of an acidophilic Rhodotorula sp. ZM1 and its phenol-degrading capability under acidic conditions.

The goal of this work was to investigate the genetics of an acidophilic phenol-degrading yeast strain using whole-genome sequencing (WGS), characterize the growth of the strain and phenol degradation capability as well as degradation pathway under extremely acidic conditions. The result showed that the strain ZM1 isolated from an acid mine drainage (AMD) belongs to basidiomycetous yeast Rhodotorula sp., which possesses some unique genes compared to other four closely related Rhodotorula species. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggested that ZM1 possessed the degradation potentials for aromatic compounds. ZM1 was acidophilic with the optimum growth at the initial pH of 3.0. It could adjust pH to desired levels probably by acid production during the cultivation. Notably, at pH 3.0, the strain ZM1 showed a high phenol-degrading capability that almost completely degraded 1100 mg/L of phenol in 120 h with the highest degradation rate of 0.074 g/(g cell dry weight h). Under the same pH, the strain could completely degrade 500 mg/L phenol within 48 h at NaCl concentration up to 10 g/L. The identification of the gene catA by the KEGG analysis, together with the presence of metabolic intermediate of cis, cis-muconic acid detected by gas chromatography-mass spectrometry, confirmed that the strain ZM1 degraded phenol via ortho-cleavage pathway. These findings suggest that the indigenous yeasts strain ZM1 could be exploited as an important member for in-situ biodegradation of aromatic compounds in the extremely acidic environments.

Aerobic degradation of 3,3',4,4'-tetrachlorobiphenyl by a resuscitated strain Castellaniella sp. SPC4: Kinetics model and pathway for biodegradation.

Resuscitated strains which were obtained by addition of resuscitation promoting factor (Rpf) could provide a vast majority of microbial source for obtaining highly efficient polychlorinated biphenyl (PCB)-degrading bacteria. In this study, the Castellaniella sp. strain SPC4 which was resuscitated by Rpf addition showed the highest efficiency in degradation of 3,3',4,4'-tetrachlorobiphenyl (PCB 77) among the resuscitated and non-resuscitated isolates. Further investigations on the PCB 77 degradation capability of the resuscitated strain SPC4 showed that SPC4 could efficiently degrade PCB 77 with maximum degradation rate (qmax) of 0.066/h at about 20 mg/L of PCB 77. The maximum growth rate on PCB 77 was 2.663 × 107 CFU/(mL·h) (0.024/h). The most suitable model of Edward demonstrated that the SPC4 could achieve qmax of 0.9315/h, with substrate-affinity of 11.33 mg/L and substrate-inhibition constants of 11.41 mg/L. Meanwhile, the presence of bphA gene expression and chlorine ions release, together with the identification of metabolites, confirmed that the bph-encoded biphenyl pathway was involved in PCB 77 mineralization by SPC4. This report is the first to demonstrate aerobic degradation of PCB 77 by the resuscitated strain Castellaniella sp. SPC4, indicating excellent potential for PCB bioremediation.

Resuscitation of viable but non-culturable bacteria to enhance the cellulose-degrading capability of bacterial community in composting.

Nowadays, much of what we know regarding the isolated cellulolytic bacteria comes from the conventional plate separation techniques. However, the culturability of many bacterial species is controlled by resuscitation-promoting factors (Rpfs) due to entering a viable but non-culturable (VBNC) state. Therefore, in this study, Rpf from Micrococcus luteus was added in the culture medium to evaluate its role in bacterial isolation and enhanced effects on cellulose-degrading capability of bacterial community in the compost. It was found that Proteobacteria and Actinobacteria were two main phyla in the compost sample. The introduction of Rpf could isolate some unique bacterial species. The cellulase activity of enrichment cultures with and without Rpf treatment revealed that Rpf treatment significantly enhanced cellulase activity. Ten isolates unique in Rpf addition displayed carboxymethyl-cellulase (CMCase) activity, while six isolates possessed filter paper cellulase (FPCase) activity. This study provides new insights into broader cellulose degraders, which could be utilized for enhancing cellulosic waste treatment.

Resuscitation of functional bacterial community for enhancing biodegradation of phenol under high salinity conditions based on Rpf.

This study assumed that key degraders of functional bacterial community were prone to enter into the viable but non-culturable (VBNC) state under high saline phenolic conditions, and resuscitation-promoting factor (Rpf) could strengthen these degraders for better performances. Based on these assumptions, Rpf was used to enhance salt-tolerant phenol-degrading capability of functional populations in activated sludge. Results suggested that Rpf accelerated the start-up process during sludge domestication, and significantly enhanced salt-tolerant phenol-degrading capability. High-throughput sequencing showed that the resuscitation and stimulation functions of Rpf linked mainly to the genus Corynebacterium within the phylum Actinobacteria, and the genera Proteiniphilum and Petrimonas within the phylum Bacteroidete. These key functional populations contributed to better phenol-degrading capabilities under high salinity conditions. This study indicated that Rpf is a promising additive for improving biological treatment performance of saline phenolic wastewater.

Influences of acid-base property of membrane on interfacial interactions related with membrane fouling in a membrane bioreactor based on thermodynamic assessment.

Failure of membrane hydrophobicity in predicting membrane fouling requires a more reliable indicator. In this study, influences of membrane acid base (AB) property on interfacial interactions in two different interaction scenarios in a submerged membrane bioreactor (MBR) were studied according to thermodynamic approaches. It was found that both the polyvinylidene fluoride (PVDF) membrane and foulant samples in the MBR had relatively high electron donor (γ(-)) component and low electron acceptor (γ(+)) component. For both of interaction scenarios, AB interaction was the major component of the total interaction. The results showed that, the total interaction monotonically decreased with membrane γ(-), while was marginally affected by membrane γ(+), suggesting that γ(-) could act as a reliable indicator for membrane fouling prediction. This study suggested that membrane modification for fouling mitigation should orient to improving membrane surface γ(-) component rather than hydrophilicity.

A new approach to construct three-dimensional surface morphology of sludge flocs in a membrane bioreactor.

In this paper, a novel approach to construct three-dimensional (3D) surface morphology of sludge flocs in a membrane bioreactor (MBR) was proposed. The new approach combined the static light scattering method for fractal dimension (Df) determination with the modified two-variable Weierstrass-Mandelbrot (WM) function based on fractal geometry and coordinate transformation for spherical surface construction. It was found that the sludge flocs in the MBR showed apparent fractal characteristics. Results showed that the constructed 3D morphology of sludge flocs was very sensitive to Df, and higher Df induced a more compact and smoother surface morphology. With a set of proper parameter data, the constructed 3D surface morphology of sludge flocs could be quite similar to the real floc surface morphology, showing the feasibility of the proposed approach. The proposed solution to floc surface construction could be potentially used in interfacial interaction assessment, giving important implications for membrane fouling research.

Quantitative assessment of interfacial interactions with rough membrane surface and its implications for membrane selection and fabrication in a MBR.

The interfacial interactions between a foulant particle and rough membrane surface in a submerged membrane bioreactor (MBR) were quantitatively assessed by using a new-developed method. It was found that the profile of total interaction versus separation distance was complicated. There were an energy barrier and two negative energy ranges in the profile. Further analysis showed that roughness scale significantly affected the strength and properties of interfacial interactions. It was revealed that there existed a critical range of roughness scale within which the total energy in the separation distance ranged from 0 to several nanometers was continually repulsive. Decrease in foulant size would increase the strength of specific interaction energy, but did not change the existence of a critical roughness scale range. These findings suggested the possibility to "tailor" membrane surface morphology for membrane fouling mitigation, and thus gave significant implications for membrane selection and fabrication in MBRs.

A novel approach for quantitative evaluation of the physicochemical interactions between rough membrane surface and sludge foulants in a submerged membrane bioreactor.

This study proposed a novel approach for quantitative evaluation of the physicochemical interactions between a particle and rough surface. The approach adopts the composite Simpson's rule to numerically calculate the double integrals in the surface element integration of these physicochemical interactions. The calculation could be achieved by a MATLAB program based on this approach. This approach was then applied to assess the physicochemical interactions between rough membrane surface and sludge foulants in a submerged membrane bioreactor (MBR). The results showed that, as compared with smooth membrane surface, rough membrane surface had a much lower strength of interactions with sludge foulants. Meanwhile, membrane surface morphology significantly affected the strength and properties of the interactions. This study showed that the newly developed approach was feasible, and could serve as a primary tool for investigating membrane fouling in MBRs.

The sorption of pentachlorophenol by aged sediment supplemented with black carbon produced from rice straw and fly ash.

Black carbon (BC) is a potential material for controlling hydrophobic organic contaminants in sediment because it has a high sorption capacity. In the present study, the sorption of pentachlorophenol (PCP) onto sediments supplemented with rice straw biochar (RC) and fly ash (FC) aged for different times and at temperatures were investigated. The sorption of PCP increased with increasing amounts of BC and decreased with aging time and storage temperature of the BC-supplemented sediments. The sorption of PCP onto RC-supplemented sediments was higher than those supplemented with FC regardless of whether or not BCs were aged in sediments. For aged sediments containing 2% BCs, the sorption capacity was 9.15- and 2.87-fold higher than that of FC when supplemented with RC aged at 25 and 45°C, respectively. Therefore, biochar is better than fly ash for controlling organic pollutants even when the RC was present in sediment for a long time.