Microbial phylogenetic relatedness links to distinct successional patterns of bacterial and fungal communities.

The mechanisms underlying microbial community dynamics and co-occurrence patterns along ecological succession are crucial for understanding ecosystem recovery but remain largely unexplored. Here, we investigated community dynamics and taxa co-occurrence patterns in bacterial and fungal communities across a well-established chronosequence of post-mining lands spanning 54 years of recovery. Bacterial community structures became increasingly phylogenetically clustered with soil age at early successional stages and varied less at later successional stages. The dynamics of bacterial community phylogenetic structures were determined by the changes in the soil vegetation cover along succession. The dynamics of fungal community phylogenetic structures did not significantly correlate with soil age, soil properties or vegetation cover, and were mainly attributed to stochastic processes. Along succession, the common decrease in the bacterial co-occurrence complexity and in the average pairwise phylogenetic distances between co-occurring bacteria implied a decrease in potential bacterial cooperation. The increased complexity of fungal co-occurrence along succession was independent of phylogenetic relatedness between co-occurring fungi. This study provides new sights into ecological mechanisms underlying bacterial and fungal community succession.

Closing the gap: examining the impact of source habitat proximity on plant and soil microbial communities in post-mining spoil heap succession.

Introduction: Revegetation of barren substrates is often determined by the composition and distance of the nearest plant community, serving as a source of colonizing propagules. Whether such dispersal effect can be observed during the development of soil microbial communities, is not clear. In this study, we aimed to elucidate which factors structure plant and soil bacterial and fungal communities during primary succession on a limestone quarry spoil heap, focusing on the effect of distance to the adjoining xerophilous grassland. Methods: We established a grid of 35 plots covering three successional stages - initial barren substrate, early successional community and late successional grassland ecosystem, the latter serving as the primary source of soil colonization. On these plots, we performed vegetation surveys of plant community composition and collected soil cores to analyze soil chemical properties and bacterial and fungal community composition. Results: The composition of early successional plant community was significantly affected by the proximity of the source late successional community, however, the effect weakened when the distance exceeded 20 m. Early successional microbial communities were structured mainly by the local plant community composition and soil chemical properties, with minimal contribution of the source community proximity. Discussion: These results show that on small spatial scales, species migration is an important determinant of plant community composition during primary succession while the establishment of soil microbial communities is not limited by dispersal and is primarily driven by local biotic and abiotic conditions.

Plant effects on microbiome composition are constrained by environmental conditions in a successional grassland.

BACKGROUND: Below-ground microbes mediate key ecosystem processes and play a vital role in plant nutrition and health. Understanding the composition of the belowground microbiome is therefore important for maintaining ecosystem stability. The structure of the belowground microbiome is largely determined by individual plants, but it is not clear how far their influence extends and, conversely, what the influence of other plants growing nearby is. RESULTS: To determine the extent to which a focal host plant influences its soil and root microbiome when growing in a diverse community, we sampled the belowground bacterial and fungal communities of three plant species across a primary successional grassland sequence. The magnitude of the host effect on its belowground microbiome varied among microbial groups, soil and root habitats, and successional stages characterized by different levels of diversity of plant neighbours. Soil microbial communities were most strongly structured by sampling site and showed significant spatial patterns that were partially driven by soil chemistry. The influence of focal plant on soil microbiome was low but tended to increase with succession and increasing plant diversity. In contrast, root communities, particularly bacterial, were strongly structured by the focal plant species. Importantly, we also detected a significant effect of neighbouring plant community composition on bacteria and fungi associating with roots of the focal plants. The host influence on root microbiome varied across the successional grassland sequence and was highest in the most diverse site. CONCLUSIONS: Our results show that in a species rich natural grassland, focal plant influence on the belowground microbiome depends on environmental context and is modulated by surrounding plant community. The influence of plant neighbours is particularly pronounced in root communities which may have multiple consequences for plant community productivity and stability, stressing the importance of plant diversity for ecosystem functioning.

Electrically conductive composite materials with incorporated waste and secondary raw materials.

Silicate composites have very low conductivity in general. It is possible to achieve an electrical resistivity decrease by adding an electro-conductive filler. The conductive mixture consists of cementitious binder, various types of silica sand, and graphite-based conductive fillers. One of the research focusses is partial substitution of ordinary raw materials by alternative components (waste materials by-products and secondary raw materials) and its influence on composite properties. The alternative components studied were fly ash as a partial binder replacement, waste graphite from two different sources and steel shavings as a substitute for conductive filler. Resistivity of cured conductive silicate-based specimens was analysed in relation to changes in physico-mechanical properties in context of microstructural changes in the hardened cementitious matrix (by optical and scanning electron microscopy with energy disperse analysis). Partial substitution of cement by fly ash was found to reduce the electrical resistivity of the composite. Some of the waste graphite fillers significantly reduce the resistivity of the cement composite and increase the compressive strength. It was proven, that is possible to replace primary conductive fillers by secondary raw materials.

New Chemically Resistant Coating Systems with Progressive Incorporation of Hazardous Waste in Polyurethane and Epoxy Matrices.

New types of highly chemically resistant coating systems, primarily intended for concrete and metal substrates, were designed and experimentally verified in the paper. Secondary raw materials in optimal amounts, including solidified hazardous waste (e.g., end product and cement bypass dust), were used as microfillers. The polymer coating systems, containing pre-treated hazardous waste (HW), showed high abrasion resistance and excellent adhesion to metal and concrete surfaces. Based on polyurethane and epoxy resins, the coatings can be used in environments where aggressive chemical media act, such as sewers and the chemical industry. The developed polymeric coating systems showed even better properties than the compared reference coating systems. The chemical resistance of the three-layer coating systems was evaluated both visually and based on changes in mechanical properties, such as hardness and adhesion. The microstructure of the coating systems was also monitored using a digital optical microscope and a scanning electron microscope with energy dispersive X-ray analysis (SEM-EDX) after chemical stress. It was observed that the particles of HW were fully incorporated into the polymer matrix of the coating systems.

Recovery of Industrial Wastes as Fillers in the Epoxy Thermosets for Building Application.

Epoxy resins are currently used in many areas of construction, such as resistant coatings, anchors, fibre-reinforced polymer (FRP) composites, grouts, etc. This paper deals mainly with epoxy composites that can be applied during the rehabilitation of concrete constructions. The influence of a filler type on epoxy thermoset composites was monitored, whilst three different types of epoxy resin were used in order to achieve a better representation and confirmation of the results. During the testing of fillers, these were mainly secondary raw materials, including pre-treated hazardous waste (neutralisation sludge), representing various shapes and sizes of particle, while their amount in the epoxy matrix was chosen with regard to optimal viscosity and workability. Physical and mechanical parameters, like compressive and flexural strengths, cohesion with the concrete and thermal expansion of the epoxy composites containing various fillers were determined. The microstructure of epoxy composites with a different filler type and chemical resistance against chemical aggressive media were all monitored. The microstructure of epoxy composites was monitored using scanning electron microscopy (SEM) supported by energy-dispersive X-ray spectroscopy (EDX). Computed tomography (CT) was also used for the evaluation of the cohesion of the epoxy composites with concrete and dispersion of the filler in the epoxy matrix.

Impact of Carbon Particle Character on the Cement-Based Composite Electrical Resistivity.

Electroconductive cement-based composites are modern materials that are commonly used in many industries such as the construction industry, among others. For example, these materials can be used as sensors for monitoring changes in construction, grounding suspension, and resistance heating materials, etc. The aim of the research presented in this article is to monitor the impact of carbon particle character on cement-based electroconductive composites. Four types of graphite were analyzed. Natural and synthetic types of graphite, with different particle sizes and one with improved electrically conductive properties, were tested. For the analysis of the electrical conductivity of powder raw materials, a new methodology was developed based on the experience of working with these materials. Various types of graphite were tested in pure cement paste (80% cement, 20% graphite) as well as in a composite matrix, which consisted of cement (16.8%), a mixture of silica sand 0-4 mm (56.4%), graphite filler (20.0%) ground limestone (6.7%) and super plasticizers (0.1%). The resistivity and physical-mechanical properties of the composite material were determined. Furthermore, the resistivity of the test samples was measured with a gradual decrease in saturation. It may be concluded that graphite fillers featuring very fine particles and high specific surface are most suitable and most effective for creating electrically conductive silicate composites. The amount, shape and, in particular, the fineness of the graphite filler particles thus creates suitable conditions for the creation of an integrated internal electricity-conductive network. In the case of the use of a coarse type of graphite or purely non-conductive fillers, the presence of an electrolyte, for example, in the form of water, is necessary to achieve a low resistivity. Samples with fine types of graphite fillers achieved stable resistivity values when the sample humidity changed. The addition of graphite fillers caused a large decrease in the strength of the samples.

Dynamics of Soil Bacterial and Fungal Communities During the Secondary Succession Following Swidden Agriculture IN Lowland Forests.

Elucidating dynamics of soil microbial communities after disturbance is crucial for understanding ecosystem restoration and sustainability. However, despite the widespread practice of swidden agriculture in tropical forests, knowledge about microbial community succession in this system is limited. Here, amplicon sequencing was used to investigate effects of soil ages (spanning at least 60 years) after disturbance, geographic distance (from 0.1 to 10 km) and edaphic property gradients (soil pH, conductivity, C, N, P, Ca, Mg, and K), on soil bacterial and fungal communities along a chronosequence of sites representing the spontaneous succession following swidden agriculture in lowland forests in Papua New Guinea. During succession, bacterial communities (OTU level) as well as its abundant (OTU with relative abundance > 0.5%) and rare (<0.05%) subcommunities, showed less variation but more stage-dependent patterns than those of fungi. Fungal community dynamics were significantly associated only with geographic distance, whereas bacterial community dynamics were significantly associated with edaphic factors and geographic distance. During succession, more OTUs were consistently abundant (n = 12) or rare (n = 653) for bacteria than fungi (abundant = 6, rare = 5), indicating bacteria were more tolerant than fungi to environmental gradients. Rare taxa showed higher successional dynamics than abundant taxa, and rare bacteria (mainly from Actinobacteria, Proteobacteria, Acidobacteria, and Verrucomicrobia) largely accounted for bacterial community development and niche differentiation during succession.

Compromised Astrocyte Swelling/Volume Regulation in the Hippocampus of the Triple Transgenic Mouse Model of Alzheimer's Disease.

In this study, we aimed to disclose the impact of amyloid-ß toxicity and tau pathology on astrocyte swelling, their volume recovery and extracellular space (ECS) diffusion parameters, namely volume fraction (α) and tortuosity (λ), in a triple transgenic mouse model of Alzheimer's disease (3xTg-AD). Astrocyte volume changes, which reflect astrocyte ability to take up ions/neurotransmitters, were quantified during and after exposure to hypo-osmotic stress, or hyperkalemia in acute hippocampal slices, and were correlated with alterations in ECS diffusion parameters. Astrocyte volume and ECS diffusion parameters were monitored during physiological aging (controls) and during AD progression in 3-, 9-, 12- and 18-month-old mice. In the hippocampus of controls α gradually declined with age, while it remained unaffected in 3xTg-AD mice during the entire time course. Moreover, age-related increases in λ occurred much earlier in 3xTg-AD animals than in controls. In 3xTg-AD mice changes in α induced by hypo-osmotic stress or hyperkalemia were comparable to those observed in controls, however, AD progression affected α recovery following exposure to both. Compared to controls, a smaller astrocyte swelling was detected in 3xTg-AD mice only during hyperkalemia. Since we observed a large variance in astrocyte swelling/volume regulation, we divided them into high- (HRA) and low-responding astrocytes (LRA). In response to hyperkalemia, the incidence of LRA was higher in 3xTg-AD mice than in controls, which may also reflect compromised K+ and neurotransmitter uptake. Furthermore, we performed single-cell RT-qPCR to identify possible age-related alterations in astrocytic gene expression profiles. Already in 3-month-old 3xTg-AD mice, we detected a downregulation of genes affecting the ion/neurotransmitter uptake and cell volume regulation, namely genes of glutamate transporters, α2ß2 subunit of Na+/K+-ATPase, connexin 30 or Kir4.1 channel. In conclusion, the aged hippocampus of 3xTg-AD mice displays an enlarged ECS volume fraction and an increased number of obstacles, which emerge earlier than in physiological aging. Both these changes may strongly affect intercellular communication and influence astrocyte ionic/neurotransmitter uptake, which becomes impaired during aging and this phenomenon is manifested earlier in 3xTg-AD mice. The increased incidence of astrocytes with limited ability to take up ions/neurotransmitters may further add to a cytotoxic environment.

Author Correction: GlobalFungi, a global database of fungal occurrences from high-throughput-sequencing metabarcoding studies.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

GlobalFungi, a global database of fungal occurrences from high-throughput-sequencing metabarcoding studies.

Fungi are key players in vital ecosystem services, spanning carbon cycling, decomposition, symbiotic associations with cultivated and wild plants and pathogenicity. The high importance of fungi in ecosystem processes contrasts with the incompleteness of our understanding of the patterns of fungal biogeography and the environmental factors that drive those patterns. To reduce this gap of knowledge, we collected and validated data published on the composition of soil fungal communities in terrestrial environments including soil and plant-associated habitats and made them publicly accessible through a user interface at https://globalfungi.com . The GlobalFungi database contains over 600 million observations of fungal sequences across > 17 000 samples with geographical locations and additional metadata contained in 178 original studies with millions of unique nucleotide sequences (sequence variants) of the fungal internal transcribed spacers (ITS) 1 and 2 representing fungal species and genera. The study represents the most comprehensive atlas of global fungal distribution, and it is framed in such a way that third-party data addition is possible.

A meta-analysis of global fungal distribution reveals climate-driven patterns.

The evolutionary and environmental factors that shape fungal biogeography are incompletely understood. Here, we assemble a large dataset consisting of previously generated mycobiome data linked to specific geographical locations across the world. We use this dataset to describe the distribution of fungal taxa and to look for correlations with different environmental factors such as climate, soil and vegetation variables. Our meta-study identifies climate as an important driver of different aspects of fungal biogeography, including the global distribution of common fungi as well as the composition and diversity of fungal communities. In our analysis, fungal diversity is concentrated at high latitudes, in contrast with the opposite pattern previously shown for plants and other organisms. Mycorrhizal fungi appear to have narrower climatic tolerances than pathogenic fungi. We speculate that climate change could affect ecosystem functioning because of the narrow climatic tolerances of key fungal taxa.