Therapy side-effects and predictive factors for preterm delivery in patients undergoing tocolysis with atosiban or ritodrine for threatened preterm labour.

The objective of this study was to compare the safety and efficacy of atosiban and ritodrine in the treatment of threatened preterm labour (TPL) and to analyse the predictive factors of preterm delivery. We retrospectively sampled data on 380 women hospitalised for TPL (24-35 weeks' gestation), in our clinic between 2004 and 2007. All were subjected to tocolysis with ritodrine and/or atosiban. Data were analysed using R (version 2.12.1), considering p < 0.05 as significant. We had 69 women treated with atosiban, 242 treated with ritodrine and 69 treated with ritodrine changed for atosiban, if adverse effects occurred. In the multivariate logistic regression, the use of atosiban vs ritodrine does not play any role in delaying delivery after 48 h or 7 days, whereas the cervical change at the digital examination, high contractions pre/post-therapy ratio, pPROM, cervical length and fibronectin result as predictive factors for both delivery before 48 h or 7 days. Maternal adverse drug effects were significantly more frequent in patients treated with ritodrine, and one single case of pulmonary oedema was observed. We found fewer side-effects in the atosiban than in the ritodrine group and no difference in efficacy. Moreover, the most predictive factors for preterm delivery were fibronectin test, pPROM, digital vaginal examination and uterine contraction persistence. We believe that predictive capacity of these tests could give the opportunity for targeting therapy and limiting drug side-effects and cost.

Differences related to aging in sensorimotor knowledge: Investigation of perceptual strength and body object interaction.

Embodied approach postulates that knowledge and conceptual representations are grounded in action and perception. In order to investigate the involvement of sensorimotor information in conceptual and cognitive processing, researchers have collected various norms in young adults. For instance, the perceptual strength (PS) assesses perceptual experience (i.e. visual, auditory, haptic, gustatory, olfactory) associated with a concept and the body-object-interaction (BOI) assesses the ease with which a human body can interact with the referent of a word. The importance of both BOI and PS in the multimodal composition of word meaning is today well recognized. However, given the sensorimotor development of the individual from childhood to later life, it is likely that different age periods are associated with different perceptual experience and capacity to interact with objects. The purpose of this research is to investigate exploratory the effect of age on PS and BOI by comparing the evaluation of 270 French language words by young adults and healthy older people. The results showed that older adults presented similar or even higher PS for some modalities (e.g. gustatory and olfactory) and in particular for certain categories of words, while the BOI decreases. In addition to the importance of adjusting the verbal stimuli used in aging studies when dealing with multimodal representations, our results will lead us to discuss the evolution of sensorimotor representations with age.

High-resolution Compton spectroscopy using x-ray microcalorimeters.

X-ray Compton spectroscopy is one of the few direct probes of the electron momentum distribution of bulk materials in ambient and operando environments. We report high-resolution inelastic x-ray scattering experiments with high momentum and energy transfer performed at a storage-ring-based high-energy x-ray light source facility using an x-ray transition-edge sensor (TES) microcalorimeter detector. The performance was compared with a silicon drift detector (SDD), an energy-resolving semiconductor detector, and Compton profiles were measured for lithium and cobalt oxide powders relevant to lithium-ion battery research. Spectroscopic analysis of the measured Compton profiles demonstrates the high-sensitivity to the low-Z elements and oxidation states. The line shape analysis of the measured Compton profiles in comparison with computed Hartree-Fock profiles is usually limited by the resolution of the semiconductor detector. We have characterized an x-ray TES microcalorimeter detector for high-resolution Compton scattering experiments using a bending magnet source at the Advanced Photon Source with a double crystal monochromator, providing monochromatic photon energies near 27.5 keV. The momentum resolution below 0.16 atomic units (a.u.) was measured, yielding an improvement of more than a factor of 7 over a state-of-the-art SDD for the same scattering geometry. Furthermore, the lineshapes of narrow valence and broad core electron profiles of sealed lithium metal were clearly resolved using an x-ray TES compared to smeared and broadened lineshapes observed when using the SDD. High-resolution Compton scattering using the energy-resolving area detector shown here presents new opportunities for spatial imaging of electron momentum distributions for a wide class of materials with applications ranging from electrochemistry to condensed matter physics.

Picosecond time-resolved laser pump/X-ray probe experiments using a gated single-photon-counting area detector.

The recent developments in X-ray detectors have opened new possibilities in the area of time-resolved pump/probe X-ray experiments; this article presents the novel use of a PILATUS detector to achieve X-ray pulse duration limited time-resolution at the Advanced Photon Source (APS), USA. The capability of the gated PILATUS detector to selectively detect the signal from a given X-ray pulse in 24 bunch mode at the APS storage ring is demonstrated. A test experiment performed on polycrystalline organic thin films of alpha-perylene illustrates the possibility of reaching an X-ray pulse duration limited time-resolution of 60 ps using the gated PILATUS detector. This is the first demonstration of X-ray pulse duration limited data recorded using an area detector without the use of a mechanical chopper array at the beamline.

The basal to total insulin ratio in outpatients with diabetes on basal-bolus regimen.

OBJECTIVE: To evaluate the basal/total ratio of daily insulin dose (b/T) in outpatients with diabetes type 1 (DM1) and type 2 (DM2) on basal-bolus regimen, by investigating whether there is a relationship with HbA1c and episodes of hypoglycemia. METHODS: Multicentric, observational, cross-sectional study in Italy. Adult DM1 (n = 476) and DM2 (n = 541) outpatients, with eGFR >30 mL/min/1.73 m2, on a basal-bolus regimen for at least six months, were recruited from 31 Italian Diabetes services between March and September 2016. Clinicaltrials.govID: NCT03489031. RESULTS: Total daily insulin dose was significantly higher in DM2 patients (52.3 ± 22.5 vs. 46 ± 20.9 U/day), but this difference disappeared when insulin doses were normalized for body weight. The b/T ratio was lower than 0.50 in both groups: 0.46 ± 0.14 in DM1 and 0.43 ± 0.15 in DM2 patients (p = 0.0011). The b/T was significantly higher in the patients taking metformin in both groups, and significantly different according to the type of basal insulin (Degludec, 0.48 in DM1 and 0.44 in DM2; Glargine, 0.44 in DM1 and 0.43 in DM2; Detemir, 0.45 in DM1 and 0.39 in DM2). The b/T ratio was not correlated in either group to HbA1c or incidence of hypoglycemia (<40 mg/dL, or requiring caregiver intervention, in the last three months). In the multivariate analysis, metformin use and age were independent predictors of the b/T ratio in both DM1 and DM2 patients, while the type of basal insulin was an independent predictor only in DM1. CONCLUSION: The b/T ratio was independent of glycemic control and incidence of hypoglycemia.

Correction to: The basal to total insulin ratio in outpatients with diabetes on basal-bolus regimen.

[This corrects the article DOI: 10.1007/s40200-018-0358-2.].

Grafting response to excess boron and expression analysis of genes coding boron transporters in tomato.

Boron (B) is essential for plant growth, however its excess in soil and/or in irrigation water can severely compromise plant growth and yield. The goal of this work was to determine whether grafting onto 'Arnold', a commercial interspecific hybrid (Solanum lycopersicum × S. habrochaites) rootstock, which in a previous study was found to be tolerant to salt stress, could improve tomato (S. lycopersicum L. 'Ikram') tolerance to excess B, and whether this effect is associated with an exclusion mechanism. Non-grafted, self-grafted and grafted plants were hydroponically grown in a greenhouse with B concentration in the nutrient solution of 0.27 (control), 5, 10 and 15 mg·l-1 . A transcription analysis was carried out on SlNIP5 and SlBOR1 genes, which encode putative B transporters. Grafting 'Ikram' onto 'Arnold' rootstock reduced B concentration in leaf tissue of plants exposed to B concentrations of 10-15 mg·l-1 . At high B levels, SlNIP5 was down-regulated in all grafting combinations, while SlBOR1 was down-regulated only in the roots of plants grafted onto 'Arnold'. We conclude that grafting the susceptible tomato cultivar 'Ikram' onto the commercial rootstock 'Arnold' improved tolerance to excess B by reducing expression of genes encoding for B transporters at the root level, thus partially reducing the root uptake of B and its accumulation in the shoot.

Tumor necrosis factor involvement in myocardial ischaemia-reperfusion injury.

The role of tumor necrosis factor-alpha was investigated in an anaesthetized rat model of coronary artery ligation (60 min) and reperfusion (MI/R). Sham-occluded rats (sham MI/R) were used as controls. Survival rate, myocardial necrosis, myocardial myeloperoxidase activity, serum creatinine kinase activity and serum and macrophage tumor necrosis factor-alpha were studied. Ischaemia-reperfusion injury significantly reduced survival rate (45%), produced marked myocardial injury, increased serum creatinine kinase activity and increased myocardial myeloperoxidase activity in the area-at-risk and in the necrotic area. Serum tumor necrosis factor-alpha was undetectable during the occlusion period, but increased significantly upon release of the coronary artery. At the end of reperfusion, macrophage tumor necrosis factor-alpha was also increased. Passive immunization with a hyperimmune serum containing antibodies against murine tumor necrosis factor-alpha significantly increased survival rate (80%), lowered myocardial necrosis, reduced the increase in serum creatinine kinase activity and decreased myeloperoxidase activity in the area-at-risk and in the necrotic area. These data are consistent with an involvement of tumor necrosis factor-alpha in myocardial ischaemia-reperfusion injury.

The effect of cloricromene, a coumarine derivative, on leukocyte accumulation, myocardial necrosis and TNF-alpha production in myocardial ischaemia-reperfusion injury.

The effects of cloricromene, a coumarine derivative, were studied in an anaesthetized rat model of coronary artery ligation (60 min) followed by reperfusion (60 min; MI/R). Sham operated rats were used as controls (Sham MI/R). Myocardial ischaemia-reperfusion injury produced a marked myocardial injury (necrotic area/area-at-risk = 68 +/- 4%; necrotic area/total area = 48 +/- 3%) high serum creatinphosphokinase activity (Sham MI/R = 29 +/- 8 U/ml; MI/R = 205 +/- 11 U/ml) and elevated myocardial myeloperoxidase activity (investigated as an index of leukocyte adhesion and accumulation), in the area-at-risk (6.3 +/- 0.2 U x 10(-3)/g tissue) and in necrotic area (6.5 +/- 0.5 U x 10(-3)/g tissue). Furthermore, serum TNF-alpha was undetectable during the occlusion period, but upon the release of the coronary artery significantly increased. At the end of reperfusion, macrophage TNF-alpha was also enhanced. The administration of cloricromene (2 mg/kg, 5 minutes after the onset of reperfusion) significantly reduced myocardial injury (necrotic area/area-at-risk 30 +/- 1.3%; necrotic area/total area = 25 +/- 1.5) blunted the increase in serum creatinphosphokinase activity (92 +/- 5 U/ml) and lowered myeloperoxidase activity in area-at-risk (2.5 +/- 0.2 U x 10(-3)/g tissue) and in necrotic area (2.2 +/- 0.3 U x 10(-3)/g tissue) and decreased the serum and macrophage levels of TNF-alpha. These data indicate that cloricromene exerts beneficial effects on myocardial ischaemia/reperfusion injury. Finally, since we measured increased serum levels of TNF-alpha that were blunted by the cloricromene treatment, our data are consistent with an involvement of TNF-alpha in the reperfusion injury induced by myocardial ischaemia.

Phenolic compounds and antioxidant activity from red grape marc extracts.

The aims of this work were to determine the amounts of the different classes of phenolic compounds in an ethanolic extract from red grape marc and its components, peels and seeds, and to evaluate their antioxidant activities by the beta-carotene bleaching test for their utilization as natural antioxidants. The results showed that red grape marc was rich in polyphenol compounds with a clear antioxidant activity. The extracts, in fact, at very low concentration (20 ppm) in total phenols showed an antioxidant activity (AA) higher than 43% on average, while at higher concentration (80-160 ppm) all the fractions had an AA comparable to that of butlylated-hydroxytoluene. Grape seeds seemed to give the highest contribution to such AA, as they contained high quantities of proanthocyanidines, a type of flavonoid known for its high antioxidant properties.

High ghrelin levels in post-treatment euthyroid patients with Hashimoto's thyroiditis: a case-control preliminary study.

RATIONALE: Hashimoto's thyroiditis is a chronic inflammatory condition often associated with changes in appetite and body composition. Ghrelin is an orexigenic peptide involved in the regulation of appetite and food intake. A possible role of ghrelin in mediating inflammation has been suggested. A few contrasting published data are available on the relationship between thyroid status and circulating ghrelin in patients affected by Hashimoto's thyroiditis. The aim of the present case-control study was to provide additional evidence on the relationship between thyroid status and plasma ghrelin levels in post-treatment euthyroid female patients with Hashimoto's thyroiditis, compared to healthy controls. METHODS: 25 women [age 46.6±10.6 years; Body Mass Index 26.3±3.8 kg/m²] affected by overt hypothyroidism due to Hashimoto's thyroiditis were studied after thyroid hormones and body weight were already normalized for at least 2 months following L-thyroxine replacement. 25 healthy women (age 40.2±6.4 years; Body Mass Index 26.2±4.0 kg/m²) served as the control group. Blood levels of thyroid hormones, thyroid peroxidase antibodies, thyroglobulin antibodies and ghrelin were determined. Fat mass, fat-free mass and high-density lipoprotein cholesterol were also assessed. RESULTS: Circulating ghrelin levels were significantly higher in patients vs. control subjects (p<0.001). No differences were found in metabolic parameters (body mass index, fat mass, fat-free mass, high-density lipoprotein cholesterol) between groups. CONCLUSION: The present study provides additional evidence of hyperghrelinemia status in post-treatment euthyroid patients affected by Hashimoto's thyroiditis.

Intraspecific variability of the essential oil of Calamintha nepeta subsp. nepeta from Southern Italy (Apulia).

The essential oil of 46 spontaneous plants of Calamintha nepeta (L.) Savi subsp. nepeta growing wild in Sud, Italy (Salento, Apulia), were investigated by GC/MS. Fifty-seven components were identified in the oil representing over the 98% of the total oil composition. Four chemotypes were identified: piperitone oxide, piperitenone oxide, piperitone-menthone and pulegone.

Corioangioma placentario gigante, polihidramnios y parto pretérmino. A propósito de un caso / Giant placental chorioangioma, polyhydramnios and preterm delivery. A case report

Presentamos el caso de una paciente que tuvo un parto pretérmino en la semana 34, y estaba diagnosticada de polihidramnios asociado a una imagen ecográfica, inicialmente clasificada como cotiledón placentario accesorio. Tras el análisis histológico se determinó que esa estructura correspondía a un corioangioma gigante, y podría, en nuestra opinión, ser el causante del cuadro clínico que desarrolló la paciente. Dado el reducido número de estudios, y las nuevas posibilidades de tratamiento, consideramos podría ser interesante la exposición del caso

We report the case of a patient with preterm labour at 34 weeks with polyhydramnios associated with an obstetric scan finding initially defined as accessory placental cotyledon. At the histological examination, this was found to be a giant chorioangioma, which in our opinion could be the cause of the patient's clinical symptoms. Due to the lack of studies in the literature and new treatment possibilities, we consider that publication of the case may be of interest

Simulations of a micro-PET system based on liquid xenon.

The imaging performance of a high-resolution preclinical micro-positron emission tomography (micro-PET) system employing liquid xenon (LXe) as the gamma-ray detection medium was simulated. The arrangement comprises a ring of detectors consisting of trapezoidal LXe time projection ionization chambers and two arrays of large area avalanche photodiodes for the measurement of ionization charge and scintillation light. A key feature of the LXePET system is the ability to identify individual photon interactions with high energy resolution and high spatial resolution in three dimensions and determine the correct interaction sequence using Compton reconstruction algorithms. The simulated LXePET imaging performance was evaluated by computing the noise equivalent count rate, the sensitivity and point spread function for a point source according to the NEMA-NU4 standard. The image quality was studied with a micro-Derenzo phantom. Results of these simulation studies included noise equivalent count rate peaking at 1326 kcps at 188 MBq (705 kcps at 184 MBq) for an energy window of 450-600 keV and a coincidence window of 1 ns for mouse (rat) phantoms. The absolute sensitivity at the center of the field of view was 12.6%. Radial, tangential and axial resolutions of (22)Na point sources reconstructed with a list-mode maximum likelihood expectation maximization algorithm were ≤0.8 mm (full-width at half-maximum) throughout the field of view. Hot-rod inserts of <0.8 mm diameter were resolvable in the transaxial image of a micro-Derenzo phantom. The simulations show that a LXe system would provide new capabilities for significantly enhancing PET images.

Ascitis fetal aislada idiopática / Idiopathic isolated foetal ascites

La ascitis fetal aislada es un cuadro poco frecuente, ya que en la mayoría de ocasiones se diagnostica formando parte del cuadro de hidrops fetal. Presentamos el caso de una secundigesta de 31 años y 32 semanas y 5 días, que acude a urgencias por el hallazgo de polihidramnios y ascitis fetal masiva aislada en una ecografía rutinaria. La gestación había tenido una evolución normal hasta el momento. Como dato relevante presentó una translucencia nucal (TN) en el primer trimestre por encima del percentil 99, con un estudio de aneuploidías y ecocardiografía precoz normales, y una ecografía a las 20 semanas sin hallazgos patológicos. Una vez ingresada la paciente se indica tocólisis, maduración pulmonar fetal, se completa el estudio ecográfico morfológico fetal, Doppler fetal incluyendo pico de velocidad sistólica de la arteria cerebral media, anticuerpos irregulares, serologías TORCH y parvovirus B19, amniocentesis diagnóstica (estudio de infecciones congénitas) y evacuadora. Las pruebas realizadas resultaron negativas, por lo que se diagnosticó de ascitis fetal aislada, finalizándose la gestación mediante cesárea a las 33 semanas y 3 días por un registro cardiotocográfico (RCTG) patológico. La ascitis no tuvo repercusión en el desarrollo neonatal

Isolated foetal ascites is uncommon since, in most instances, it is diagnosed as being part of a clinical condition known as hydrops fetalis. We report the case of a 31-year-old secundigravida of 32 weeks and 5 days who came to A&E due to the finding of isolated massive foetal ascites and polyhydramnios in a routine ultrasound. The pregnancy had had a normal course until this point. Relevant data include a nuchal translucency (NT) in the first trimester above the 99th percentile with aneuploidy screening, normal early echocardiography and ultrasound at 20 weeks without pathological findings. Once the patient was admitted to hospital, tocolysis and foetal lung maturation were indicated as well as completion of a foetal morphological ultrasound study, foetal Doppler including middle cerebral artery peak systolic velocity, irregular antibodies, TORCH and parvovirus B19 serological tests, diagnostic (study of congenital infections) and reduction amniocentesis. The tests were negative; therefore the diagnosis was isolated foetal ascites. The pregnancy was terminated by caesarean section at 33 weeks and 3 days due to pathological cardiotocography (CTG) findings. The ascites had no effect on neonatal developmenT

Biochemical characterization of a lipase from olive fruit (Olea europaea L.).

Lipase (triacylglycerol acylhydrolase; EC 3.1.1.3) is the first enzyme of the degradation path of stored triacylglycerols (TAGs). In olive fruits, lipase may determine the increase of free fatty acids (FFAs) which level is an important index of virgin olive oil quality. However, despite the importance of virgin olive oil for nutrition and human health, few studies have been realized on lipase activity in Olea europaea fruits. In order to characterize olive lipase, fruits of the cv. Ogliarola, widely diffused in Salento area (Puglia, Italy), were harvested at four stages of ripening according to their skin colour (green, spotted I, spotted II, purple). Lipase activity was detected in the fatty layer obtained after centrifugation of the olive mesocarp homogenate. The enzyme exhibited a maximum activity at pH 5.0. The addition of calcium in the lipase assay medium leads to an increment of activity, whereas in the presence of copper the activity was reduced by 75%. Furthermore, mesocarp lipase activity increases during olive development but declined at maturity (purple stage). The data represent the first contribution to the biochemical characterization of an olive fruit lipase associated to oil bodies.

[Augmentation of the biodisposition of simple sugars from sorghum biomass mediated with biotechnologic processes]. / Aumento della disponibilità di zuccheri semplici da biomasse di sorgo zuccherino mediante processi biotecnologici.

The results of the enzymatic hydrolysis of pectin, hemicellulose and cellulose in the biomass of sweet sorghum (Sorghum vulgare var. saccharatum, L.) are reported. Some commercial enzymatic preparations were used: Maxazym CL 2000 (with prevailing cellulase activity), Rapidase C 80 (with prevailing pectinase activity) Rohament PC (mainly with pectinase and cellulase activities) and Rohament O (mainly with pectinase and hemicellulase activities). The treatment with Rohament PC, Rohament O and Rapidase C 80 gives an increase of the glucose content higher than the effect induced by Maxazym CL 2000. On the other hand, the cellulase and pectinase combined treatment (Maxazym CL 2000 + Rapidase C 80 or Maxazym CL 2000 + Rohament O) shows a good synergistic effect in the degradation of the plant cell wall cellulosic material.

[Characterization of the biomass of the stems of sweet sorghum]. / Caratterizzazione della biomassa dei culmi di sorgo zuccherino.

In this study the amount of glucose, sucrose and fructose was determined in the water soluble fraction while cellulose, hemicellulose, pectin and lignin contents were determined in the alcohol insoluble fraction after hydrolysis. Stalks of sweet sorghum (Sorghum vulgare L., var. saccharatum) cv. Vespa, Soave, Roce and MN 1500 at the physiological ripeness stage were used. The results of the analysis of variance with the least significant difference method (LSD, = 0.05) show that cv. Vespa and Roce have a significantly higher total amount of glucose, fructose and sucrose and at the same time, a lower cellulose, pectin, hemicellulose and lignin content then cv. Soave and cv. MN 1500.

Pigment production from in vitro cultures of Alkanna tinctoria Tausch.

An in vitro culture of Alkanna tinctoria Tausch cells was set up in order to investigate the possibility of producing alkannin, a red naphthoquinone naturally present in the root bark of this plant. Furthermore, an in vitro culture of callusderived roots was established and the production of alkannin evaluated. In the different experimental conditions investigated, differences in the production of alkannin derivatives as well as in the type of pigments produced, were observed. The potential use of this technology is discussed.