RESUMO
The emergence of multidrug-resistant bacteria is a major global health concern. The search for new therapies has brought bacteriophages into the spotlight, and new phages are being described as possible therapeutic agents. Among the bacteria that are most extensively resistant to current antibiotics is Klebsiella pneumoniae, whose hypervariable extracellular capsule makes treatment particularly difficult. Here, we describe two new K. pneumoniae phages, πVLC5 and πVLC6, isolated from environmental samples. These phages belong to the genus Drulisvirus within the family Podoviridae. Both phages encode a similar tail spike protein with putative depolymerase activity, which is shared among other related phages and probably determines their ability to specifically infect K. pneumoniae capsular types K22 and K37. In addition, we found that phage πVLC6 also infects capsular type K13 and is capable of striping the capsules of K. pneumoniae KL2 and KL3, although the phage was not infectious in these two strains. Genome sequence analysis suggested that the extended tropism of phage πVLC6 is conferred by a second, divergent depolymerase. Phage πVLC5 encodes yet another putative depolymerase, but we found no activity of this phage against capsular types other than K22 and K37, after testing a panel of 77 reference strains. Overall, our results confirm that most phages productively infected one or few Klebsiella capsular types. This constitutes an important challenge for clinical applications.
Assuntos
Bacteriófagos/genética , Bacteriófagos/isolamento & purificação , Klebsiella pneumoniae/virologia , Proteínas Virais/genética , Sequência de Aminoácidos , Antígenos Virais/química , Antígenos Virais/imunologia , Bacteriólise , Bacteriófagos/classificação , Bacteriófagos/ultraestrutura , Biologia Computacional/métodos , Variação Genética , Genoma Viral , Especificidade de Hospedeiro , Infecções por Klebsiella/microbiologia , Modelos Moleculares , Conformação Molecular , Anotação de Sequência Molecular , Fenótipo , Filogenia , Proteínas Virais/química , Sequenciamento Completo do GenomaRESUMO
Despite their simplicity, viruses can display social-like interactions such as cooperation, communication, and cheating. Focusing on bacteriophages, here we review features including viral product sharing, cooperative evasion of antiviral defenses, prudent host exploitation, superinfection exclusion, and inter-phage peptide-mediated signaling. We argue that, in order to achieve a better understanding of these processes, their mechanisms of action need to be considered in the context of social evolution theory, paying special attention to key population-level factors such as genetic relatedness and spatial structure.