RESUMO
The currently used pertussis vaccines are highly efficacious; however, neonates are susceptible to whooping cough up to the sixth month. In agreement, DTP-immunized neonate mice were not protected against intracerebral challenge with Bordetella pertussis. Neonate mice immunized with either DTP or a recombinant-BCG strain expressing the genetically detoxified S1 subunit of pertussis toxin do not show a humoral immune response against PT. On the other hand, rBCG-Pertussis induces higher PT-specific IFN-gamma production and an increase in both IFN-gamma(+) and TNF-alpha(+)-CD4(+)-T cells than the whole cell pertussis vaccine and confers protection against a lethal intracerebral challenge with B. pertussis.
Assuntos
Bordetella pertussis/imunologia , Mycobacterium bovis/genética , Toxina Pertussis/imunologia , Vacina contra Coqueluche/imunologia , Coqueluche/prevenção & controle , Animais , Anticorpos Antibacterianos/sangue , Bordetella pertussis/genética , Linfócitos T CD4-Positivos/imunologia , Vacina contra Difteria, Tétano e Coqueluche/imunologia , Humanos , Recém-Nascido , Interferon gama/biossíntese , Camundongos , Toxina Pertussis/genética , Vacina contra Coqueluche/genética , Análise de Sobrevida , Fator de Necrose Tumoral alfa/biossíntese , Coqueluche/imunologiaRESUMO
The currently used pertussis vaccines are highly efficacious; however, neonates are susceptible to whooping cough up to the sixth month. In agreement, DTP-immunized neonate mice were not protected against intracerebral challenge with Bordetella pertussis. Neonate mice immunized with either DTP or a recombinant-BCG strain expressing the genetically detoxified S1 subunit of pertussis toxin do not show a humoral immune response against PT. On the other hand, rBCG-Pertussis induces higher PT-specific IFN-ã production and an increase in both IFN-ã+ and TNF-á+-CD4+-T cells than the whole cell pertussis vaccine and confers protection against a lethal intracerebral challenge with B. pertussis