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Within this work we have investigated spiro-based tetrathiafulvalenes (TTFs) obtained as mixtures of stereoisomers from racemic spiro[5.5]undeca-1,8-dien-3-one. Compared to previously described spiro-TTFs, enantiomeric and diastereoisomeric forms have been here separated by chiral HPLC and fully characterized both experimentally and theoretically. The two types of spiro-based chiral derivatives contain either one (2) or three (1) chiral centres out of each one is spiro-type. Experimental CD, supported by TD-DFT calculations, shows differences in the optical activity between the 1 and 2 and their intermediates. The low optical activity of 2 and 3 (spiro alone chirality) was attributed to the presence of two conformers in the solution (ax and eq) of opposite Cotton effect whereas in the case of 1 and 5 (spiro and stereogenic centres) the spiro chirality seems to be responsible of the Cotton effect in the high energy region whereas the R and S chirality in the low energy region. Racemic and enantiopure forms have been successfully used for the synthesis of charge transfer complexes with tetracyanoquinodimethane (TCNQ) based acceptors.
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Invited for the cover of this issue is the collaborative research team coordinated by Arie vanâ derâ Lee at the University of Montpellier. The image depicts chiral channels with highly mobile water molecules resulting from the robust self-organization of a simple achiral acetamide. Fully reversible release and re-uptake of water molecules takes place near ambient conditions, with efficient water transport and a good selectivity against NaCl suggesting it to be an efficient candidate for desalination processes. Read the full text of the article at 10.1002/chem.20200383.
Assuntos
Aquaporinas , Água , AcetamidasRESUMO
Achiral 2-hydroxy-N-(diphenylmethyl)acetamide (HNDPA) crystallizes in the P61 chiral space group as a hydrate, building up permeable chiral crystalline helical water channels. The crystallization-driven chiral self-resolution process is highly robust, with the same air-stable crystalline form readily obtained under a variety of conditions. Interestingly, the HNDPA supramolecular helix inner pore is filled by a helical water wire. The whole edifice is mainly stabilized by robust hydrogen bonds involving the HNDPA amide bonds and CH π interactions between the HNDPA phenyl groups. The crystalline structure shows breathing behavior, with completely reversible release and re-uptake of water inside the chiral channel under ambient conditions. Importantly, the HNDPA channel is able to transport water very efficiently and selectively under biomimetic conditions. With a permeability per channel of 3.3 million water molecules per second in large unilamellar vesicles (LUV) and total selectivity against NaCl, the HNDPA channel is a very promising functional nanomaterial for future applications.
Assuntos
Aquaporinas , Água , Acetamidas , Cristalização , Ligação de Hidrogênio , Água/químicaRESUMO
The nature of the E-O chemical bond (E = C, Si, Ge, Sn) is investigated in a wide range of model derivatives, such as oxonium cations, hydrogenated/methylated/fluorinated/chlorinated ethers and acyclic oligomers incorporating the E-O-E moiety. By means of density functional theory (DFT) calculations and natural bond orbital (NBO) techniques, we propose a bonding mechanism that explains the structural contrast between the organic and the inorganic counterparts of all these derivatives: the interplay between stabilizing interactions like LP(O)âσ*(E-X) hyperconjugations and LP(O)âd(E) donations with LP(O)â¯σ(E-X) vicinal Pauli repulsions (X = H, C, O, F, Cl) dictates the equilibrium structures in terms of E-O-E angles and E-O bond lengths. In addition, the present work represents the first study of oxonium ions that describes the structural discrepancies among organic derivatives and their heavier analogues. Another novel outcome for ethers and oligomers is that the two non-equivalent lone pair electrons (LPs) at the oxygen atoms impact in different manners the geometries of such derivatives, i.e. the s/p LP is correlated with the bending behaviour of the E-O-E units, while the pure p LP mainly dictates the short E-O bond distances of inorganic derivatives. Lastly, we evaluate the impact of the number of electronegative substituents, e.g. F, Cl or OEH3 groups, on the bond patterns developed for hydrogenated or methylated ethers.
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In the present study, we evaluated for the first time the photoprotective effect of fish bone bioactive peptides (FBBP) preparation isolated from silver carp (Hypophthalmichthys molitrix) discarded tissue using in vitro experimental models of skin cells exposed to ultraviolet B (UVB) irradiation and stressing agents. FBBP preparation was obtained by papain treatment of minced bones and centrifugal ultrafiltration, and the molecular weight (MW) distribution was characterized by size exclusion and reversed-phase high performance liquid chromatography (RP-HPLC). In vitro assessment of the effect of FBBP pretreatment in UVB-irradiated L929 fibroblasts and HaCaT keratinocytes revealed their cytoprotective activity. Their capacity to efficiently reduce reactive oxygen species (ROS) production and lipid peroxidation varied in a dose-dependent manner, and it was greater in fibroblasts. A decrease of proinflammatory cytokines secretion, in particular of tumor necrosis factor alpha (TNF-α), was found after FBBP pretreatment of THP-1-derived inflamed macrophages. Melanin production and tyrosinase activity investigated in UVB-irradiated Mel-Juso cells were lowered in direct relation to FBBP concentrations. FBBP fractions with high radical scavenging activity were separated by ion exchange chromatography, and two collagenic sequences were identified. All these results offer new scientific data on aquaculture fish bone-derived peptides confirming their ability to control the antioxidant, anti-inflammatory and pigmentation processes developed during UV irradiation of skin cells and recommend their use as valuable natural ingredients of photoprotective cosmeceutical products.
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Osso e Ossos/efeitos dos fármacos , Inflamação/patologia , Peptídeos/farmacologia , Pigmentação , Pele/efeitos da radiação , Raios Ultravioleta , Animais , Antioxidantes/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Citocinas/metabolismo , Citoproteção/efeitos dos fármacos , Citoproteção/efeitos da radiação , Peixes , Células HaCaT/efeitos dos fármacos , Células HaCaT/efeitos da radiação , Humanos , Mediadores da Inflamação/metabolismo , Espaço Intracelular/metabolismo , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Malondialdeído/metabolismo , Melaninas/biossíntese , Camundongos , Peso Molecular , Oxirredução/efeitos dos fármacos , Oxirredução/efeitos da radiação , Peptídeos/isolamento & purificação , Pigmentação/efeitos dos fármacos , Pigmentação/efeitos da radiação , Espécies Reativas de Oxigênio/metabolismo , Espectrofotometria Ultravioleta , Células THP-1RESUMO
The contrasting geometrical features between organic and inorganic counterparts of amines and oxanes are explained in terms of an offset between attractive (donor-acceptor) and repulsive (donor-donor) interactions. Natural bond orbital (NBO) calculations carried out at the density functional theory level of theory reveal that hyperconjugative effects in the organic amines and ethers are overcome by repulsive interactions occurring between the lone pair on the nitrogen/oxygen atom and the adjacent σ(C-R) bond orbitals. Although displaying lower energies than in the corresponding organic derivatives, the LP(X) â σ*(E-R) (X = N, O; E = Si, Ge, Sn) interactions in heavier counterparts overcome the LP(X)···σ(E-R) repulsions, impacting thus their structural behavior. In addition, NBO deletion optimizations emphasize that among hyperconjugations, back-bonding effects of the LP(X) â d(E) type dictate to a lesser extent the anomalous structures of the inorganic amines and oxanes.
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Explaining the nature of the E-O chemical bond (E = Si, Ge, Sn) has been a great challenge for theoretical chemists during the last decades. Among the large number of models used for this purpose, the one based on hyperconjugative interactions sheds more light on the nature of chemical bonding in siloxanes. Starting from this concept, this study aimed to evaluate the impact of siloxane type hyperconjugative effects on the structural features of germoxanic and stannoxanic species and in addition to assess if p-d-like back-bonding interactions can also play important roles in determining the particular structures of these heavier analogues of ethers. Natural bond orbital deletion (NBO DEL) optimizations, carried out at the DFT level of theory, revealed that hyperconjugative effects dictate to a large extent the structural behavior of these species. Furthermore, this study points out that p-d back-bonding interactions also influence the equilibrium geometry of these species, although acting as a secondary electronic effect within the E-O-E moieties (E = Si, Ge, Sn).
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The steric and electronic effects of specific ligands can play crucial roles in stabilizing unsaturated tetrylene species. In this work, hybrid density functional theory (DFT) methods, quantum theory of atoms in molecules (QTAIM) investigations and natural bond orbital (NBO) calculations are employed to evaluate the stabilization of low-valent E(ii) centers (E = Si, Ge, Sn, Pb) through the chelating effect generated by an electron-rich ligand containing the P[double bond, length as m-dash]C-P[double bond, length as m-dash]X moiety (X = O or S). Based on several types of analyses, such as the bond dissociation energy (BDE) or the interplay between attractive (i.e., charge-transfer) and repulsive (i.e., Pauli-exchange) effects, we highlight that the stabilization energy induced by chelation is up to ca. 70 kcal mol-1 for silylenes, yet slightly decreases within the heavier analogues. Moreover, it is emphasized that chelate-stabilized silylenes can form highly stable hybrid metal-metalloid complexes with transition metals (e.g., gold). Due to push-pull effects occurring in the XâSi(ii)âAu fragment, the Si(ii)âAu bonding is significantly stronger than the XâAu, P(sp2)âAu or π(C[double bond, length as m-dash]P)âAu donor-acceptor bonds, which are potentially formed by the electron-rich P[double bond, length as m-dash]C-P[double bond, length as m-dash]X unit with the AuCl fragment. These findings are supported by energy decomposition analysis (EDA) calculations.
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Formation of stable carbides during CO bond dissociation on small ruthenium nanoparticles (RuNPs) is demonstrated, both by means of DFT calculations and by solid state 13C NMR techniques. Theoretical calculations of chemical shifts in several model clusters are employed in order to secure experimental spectroscopic assignations for surface ruthenium carbides. Mechanistic DFT investigations, carried out on a realistic Ru55 nanoparticle model (â¼1 nm) in terms of size, structure and surface composition, reveal that ruthenium carbides are obtained during CO hydrogenation. Calculations also indicate that carbide formation via hydrogen-assisted hydroxymethylidyne (COH) pathways is exothermic and occurs at reasonable kinetic cost on standard sites of the RuNPs, such as 4-fold ones on flat terraces, and not only in steps as previously suggested. Another novel outcome of the DFT mechanistic study consists of the possible formation of µ6 ruthenium carbides in the tip-B5 site, similar examples being known only for molecular ruthenium clusters. Moreover, based on DFT energies, the possible rearrangement of the surface metal atoms around the same tip-site results in a µ-Ru atom coordinated to the remaining RuNP moiety, reminiscent of a pseudo-octahedral metal center on the NP surface.
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In the context of helical chirality, bridging of biphenyl units leads to banister-type compounds and the stability of the resulted atropisomers may increase dramatically if suitable changes are performed in the linker unit that coils around the biphenyl moiety. A rigorous density functional theory (DFT) study was conducted for macrocycles containing rigid oxime ether segments connected to the biphenyl backbone in order to determine how the rotation barriers are influenced by the presence of either a flexible oligoethyleneoxide or a more rigid m-xylylene component in the macrocycle. The calculated values for the racemization barrier were in good agreement with those obtained experimentally and confirm the benefit of introducing a more rigid unit in the macrocycle on the stability of atropisomers. Solid-state data were obtained and computed data were used to assess the contribution brought by supramolecular associations observed in the lattice to the stabilization of the crystal structure. Beside introducing rigidity in the linker, complexation of flexible macrocycles with alkali metal ions is also contributing to the stability of atropisomers, leading to values for the racemization barrier matching that of the rigid macrocycle. Using diethylammonium cation as guest for the macrocycle, a spectacular increase in the barrier to rotation was observed for the resulted pseudo[2]rotaxane.
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New chiral heteroleptic germanium(ii) and tin(ii) metallylenes were obtained using 1-(para-tolylsulfinyl)-3-tosyl-5-tert-butyl-benzene as a non-symmetric O,C,O-chelating pincer ligand. Crystallographic analysis and DFT calculations indicate that the non-symmetric sulfinyl-sulfonyl pincer ligand acts as an O,C,O-coordinating pincer-type-ligand with predominant sulfinyl intramolecular S[double bond, length as m-dash]O coordination to germanium(ii) and tin(ii) centers.
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The exposure to radon and radon decay products in homes and at workplaces represents the greatest risk from natural ionizing radiation. The present study brings forward the residential, soil and water radon surveys in 5 counties of Romania. Indoor radon measurements were performed by using CR-39 track detectors exposed for 3 months on ground-floor level of dwellings, according to the NRPB Measurements Protocol. Radon concentrations in soil and water were measured using the LUK3C device. The indoor radon concentrations ranged from 5 to 2592 Bqâ m-3 with an updated preliminary arithmetic mean of 133 Bqâ m-3, and a geometric mean of 90 Bqâ m-3. In about 6% of the investigated grid cells the indoor radon concentrations exceed the threshold of 300 Bqâ m-3. The soil gas radon concentration varies from 0.8 to 169 kBqâ m-3, with a geometric mean of 28.4 kBqâ m-3. For water samples, the results show radon concentrations within the range of 0.3-352 kBqâ m-3 with a geometric mean of 7.7 Bqâ L-1. The indoor radon map was plotted on a reference grid developed by JRC with the resolution 10 × 10 km2.