Analysis of the Adherent Cell Response to the Substrate Stiffness Using Tensegrity.

Cell's shape is dependent on the cytoskeleton mechanical properties. Hybrid models were developed that combine the discrete structure for the cytoskeleton and continuum parts for other cell organelles. Tensegrity-based structures that consist of tensile and compression elements are useful models to understand the cytoskeleton mechanical behavior. In this study, we are looking to examine the reaction of the cell to a variety of substrate stiffnesses and explain the relationship between cell behavior and substrate mechanical properties. However, which tensegrity structure is appropriate for modeling a living cell? Is the structure's complexity play a major role? We used two spherical tensegrities with different complexities to assess the impact of the structure on the cell's mechanical response versus substrate's stiffness. Six- and twelve-strut tensegrities together with membrane, cytoplasm, nucleoskeleton, and nucleus envelope were assembled in Abaqus package to create a hybrid cell model. A compressive load was applied to the cell model and the reaction forces versus deflection curves were analyzed for number of substrate stiffness values. By analyzing the difference due to two different tensegrities it became clear that the lower density structure is a better choice for modeling stiffer cells. It was also found that the six-strut tensegrity is sensitive to higher range of substrate stiffness.

Electrospun halloysite nanotube loaded polyhydroxybutyrate-starch fibers for cartilage tissue engineering.

Articular cartilage is a connective load-bearing tissue with a low rate of regeneration due to slow metabolism. Fabricating tissue-like structure modified based on natural features can improve healing process. Fibrous scaffolds based on the composition of hydrophobic polyhydroxybutyrate (PHB) and hydrophilic starch reinforced using halloysite nanotubes (HNTs) with appropriate physico-chemical and biological properties was produced via electrospinning technique for long-term applications like cartilage regeneration. Textural properties were analyzed through SEM imaging that showed incorporating HNTs up to 2 wt% decreased mean fiber diameter to 158 ± 48 nm with larger pore size and appropriate porosity percentage. Moreover, the tensile strength was improved up to 4.21 ± 0.31 MPa after HNTs incorporation support chondrocyte cell growth. Furthermore, incorporating HNTs induced surface hydrophilicity and in vitro degradation. The biological assays both MTT assay and cell attachment of chondrocyte cells on 2 wt% HNTs incorporated into PHB-starch fibers indicated that HNTs incorporation can support cell growth and attachment without any toxicity for biomedical applications. To conclude, the obtained results demonstrated PHB-starch/HNTs fibrous scaffold could be potential for further experimental studies for tissue engineering applications like cartilage.

Potential of novel electrospun core-shell structured polyurethane/starch (hyaluronic acid) nanofibers for skin tissue engineering: In vitro and in vivo evaluation.

The biomaterials with excellent biocompatibility and biodegradability ¬can lead to satisfactory wound healing. In this study, core-shell structured PU (polyurethane)/St (Starch) and PU/St (Hyaluronic Acid (HA)) nanofibers were fabricated with coaxial electrospinning technique. The morphology characterization of the core-shell structure of nanofibers was investigated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images. Contact-angle measurements were confirmed the core/shell structure of the electrospun nanofibers with shell and core feed rates of 0.675 L/min and <0.135 L/min, respectively. The average fiber diameter values were calculated for polyurethane nanofibers (836 ± 172.13 nm), PU/St nanofibers (612 ± 93.21 nm) and PU/St (HA) nanofibers (428 ± 78.32 nm). The average porosity values of scaffolds were determined for PU (1.251 ± 0.235 µm), PU/St (1.734 ± 0.284 µm) and PU/St (HA) (3.186 ± 0.401 µm). The core-shell PU/St and PU/St (HA) nanofibers were evaluated in vitro by using mouse fibroblasts (L929) cells. Cell morphology and viability results were exhibited significant enhancement in cell promoting and cell attachment. Furthermore, in vivo studies was indicated Core-shell PU/St (HA) wound dressing can be an appropriate candidate for skin tissue engineering and wound healing.