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1.
Arch Microbiol ; 203(8): 4755-4776, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34370077

RESUMO

Bacteria are rich in a wide variety of secondary metabolites, such as pigments, alkaloids, antibiotics, and others. These bioactive microbial products serve a great application in human and animal health. Their molecular diversity allows these natural products to possess several therapeutic attributes and biological functions. That's why the current natural drug industry focuses on uncovering all the possible ailments and diseases that could be combated by bacterial extracts and their secondary metabolites. In this paper, we review the major utilizations of bacterial natural products for the treatment of cancer, inflammatory diseases, allergies, autoimmune diseases, infections and other diseases that threaten public health. We also elaborate on the identified biological activities of bacterial secondary metabolites including antibacterial, antifungal, antiviral and antioxidant activities all of which are essential nowadays with the emergence of drug-resistant microbial pathogens. Throughout this review, we discuss the possible mechanisms of actions in which bacterial-derived biologically active molecular entities could possess healing properties to inspire the development of new therapeutic agents in academia and industry.


Assuntos
Anti-Infecciosos , Produtos Biológicos , Animais , Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Antifúngicos , Bactérias , Produtos Biológicos/farmacologia , Humanos
2.
Biochim Biophys Acta ; 1851(8): 1040-51, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25660725

RESUMO

Sphingolipids comprise a wide variety of molecules containing a sphingoid long-chain base that can be N-acylated. These lipids are particularly abundant in the central nervous system, being membrane components of neurons as well as non-neuronal cells. Direct evidence that these brain lipids play critical functions in brain physiology is illustrated by the dramatic consequences of genetic disturbances of their metabolism. Inherited defects of both synthesis and catabolism of sphingolipids are now identified in humans. These monogenic disorders are due to mutations in the genes encoding for the enzymes that catalyze either the formation or degradation of simple sphingolipids such as ceramides, or complex sphingolipids like glycolipids. They cause varying degrees of central nervous system dysfunction, quite similarly to the neurological disorders induced in mice by gene disruption of the corresponding enzymes. Herein, the enzyme deficiencies and metabolic alterations that underlie these diseases are reviewed. Their possible pathophysiological mechanisms and the functions played by sphingolipids one can deduce from these conditions are discussed. This article is part of a Special Issue entitled Brain Lipids.


Assuntos
Glicosídeo Hidrolases/deficiência , Transtornos do Metabolismo dos Lipídeos/metabolismo , Metabolismo dos Lipídeos/genética , Doenças do Sistema Nervoso/metabolismo , Esfingolipídeos/metabolismo , Animais , Expressão Gênica , Glicosídeo Hidrolases/genética , Humanos , Transtornos do Metabolismo dos Lipídeos/genética , Transtornos do Metabolismo dos Lipídeos/patologia , Camundongos , Mutação , Doenças do Sistema Nervoso/genética , Doenças do Sistema Nervoso/patologia , Ratos , Esfingolipídeos/química
3.
Nat Commun ; 15(1): 4214, 2024 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-38760332

RESUMO

The liver gene expression of the peroxisomal ß-oxidation enzyme acyl-coenzyme A oxidase 1 (ACOX1), which catabolizes very long chain fatty acids (VLCFA), increases in the context of obesity, but how this pathway impacts systemic energy metabolism remains unknown. Here, we show that hepatic ACOX1-mediated ß-oxidation regulates inter-organ communication involved in metabolic homeostasis. Liver-specific knockout of Acox1 (Acox1-LKO) protects mice from diet-induced obesity, adipose tissue inflammation, and systemic insulin resistance. Serum from Acox1-LKO mice promotes browning in cultured white adipocytes. Global serum lipidomics show increased circulating levels of several species of ω-3 VLCFAs (C24-C28) with previously uncharacterized physiological role that promote browning, mitochondrial biogenesis and Glut4 translocation through activation of the lipid sensor GPR120 in adipocytes. This work identifies hepatic peroxisomal ß-oxidation as an important regulator of metabolic homeostasis and suggests that manipulation of ACOX1 or its substrates may treat obesity-associated metabolic disorders.


Assuntos
Acil-CoA Oxidase , Fígado , Camundongos Knockout , Obesidade , Animais , Fígado/metabolismo , Camundongos , Acil-CoA Oxidase/metabolismo , Acil-CoA Oxidase/genética , Obesidade/metabolismo , Obesidade/genética , Masculino , Resistência à Insulina , Camundongos Endogâmicos C57BL , Oxirredução , Metabolismo dos Lipídeos , Tecido Adiposo/metabolismo , Dieta Hiperlipídica , Metabolismo Energético , Ácidos Graxos/metabolismo , Receptores Acoplados a Proteínas G/metabolismo , Receptores Acoplados a Proteínas G/genética
4.
Nat Commun ; 14(1): 3278, 2023 06 13.
Artigo em Inglês | MEDLINE | ID: mdl-37311757

RESUMO

Environmental factors may alter the fetal genome to cause metabolic diseases. It is unknown whether embryonic immune cell programming impacts the risk of type 2 diabetes in later life. We demonstrate that transplantation of fetal hematopoietic stem cells (HSCs) made vitamin D deficient in utero induce diabetes in vitamin D-sufficient mice. Vitamin D deficiency epigenetically suppresses Jarid2 expression and activates the Mef2/PGC1a pathway in HSCs, which persists in recipient bone marrow, resulting in adipose macrophage infiltration. These macrophages secrete miR106-5p, which promotes adipose insulin resistance by repressing PIK3 catalytic and regulatory subunits and down-regulating AKT signaling. Vitamin D-deficient monocytes from human cord blood have comparable Jarid2/Mef2/PGC1a expression changes and secrete miR-106b-5p, causing adipocyte insulin resistance. These findings suggest that vitamin D deficiency during development has epigenetic consequences impacting the systemic metabolic milieu.


Assuntos
Diabetes Mellitus Tipo 2 , Resistência à Insulina , MicroRNAs , Deficiência de Vitamina D , Humanos , Animais , Camundongos , Diabetes Mellitus Tipo 2/genética , Células-Tronco Hematopoéticas , Deficiência de Vitamina D/complicações , Deficiência de Vitamina D/genética , Vitamina D
5.
Oncotarget ; 7(44): 71873-71886, 2016 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-27708249

RESUMO

The infiltration of melanoma tumors by macrophages is often correlated with poor prognosis. However, the molecular signals that regulate the dialogue between malignant cells and the inflammatory microenvironment remain poorly understood. We previously reported an increased expression of sphingosine kinase-1 (SK1), which produces the bioactive lipid sphingosine 1-phosphate (S1P), in melanoma. The present study aimed at defining the role of tumor SK1 in the recruitment and differentiation of macrophages in melanoma. Herein, we show that downregulation of SK1 in melanoma cells causes a reduction in the percentage of CD206highMHCIIlow M2 macrophages in favor of an increased proportion of CD206lowMHCIIhigh M1 macrophages into the tumor. This macrophage differentiation orchestrates T lymphocyte recruitment as well as tumor rejection through the expression of Th1 cytokines and chemokines. In vitro experiments indicated that macrophage migration is triggered by the binding of tumor S1P to S1PR1 receptors present on macrophages whereas macrophage differentiation is stimulated by SK1-induced secretion of TGF-ß1. Finally, RNA-seq analysis of human melanoma tumors revealed a positive correlation between SK1 and TGF-ß1 expression. Altogether, our findings demonstrate that melanoma SK1 plays a key role in the recruitment and phenotypic shift of the tumor macrophages that promote melanoma growth.


Assuntos
Macrófagos/fisiologia , Melanoma/imunologia , Fosfotransferases (Aceptor do Grupo Álcool)/fisiologia , Animais , Linhagem Celular Tumoral , Movimento Celular , Polaridade Celular , Proliferação de Células , Regulação para Baixo , Humanos , Melanoma/patologia , Camundongos , Camundongos Endogâmicos C57BL , Receptores de Lisoesfingolipídeo/fisiologia , Receptores de Esfingosina-1-Fosfato , Fator de Crescimento Transformador beta1/fisiologia
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