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1.
Lett Appl Microbiol ; 59(6): 594-603, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25099389

RESUMO

UNLABELLED: We propose a model, based on the Gompertz equation, to describe the growth of yeasts colonies on agar medium. This model presents several advantages: (i) one equation describes the colony growth, which previously needed two separate ones (linear increase of radius and of the squared radius); (ii) a similar equation can be applied to total and viable cells, colony area or colony radius, because the number of total cells in mature colonies is proportional to their area; and (iii) its parameters estimate the cell yield, the cell concentration that triggers growth limitation and the effect of this limitation on the specific growth rate. To elaborate the model, area, total and viable cells of 600 colonies of Saccharomyces cerevisiae, Debaryomyces fabryi, Zygosaccharomyces rouxii and Rhodotorula glutinis have been measured. With low inocula, viable cells showed an initial short exponential phase when colonies were not visible. This phase was shortened with higher inocula. In visible or mature colonies, cell growth displayed Gompertz-type kinetics. It was concluded that the cells growth in colonies is similar to liquid cultures only during the first hours, the rest of the time they grow, with near-zero specific growth rates, at least for 3 weeks. SIGNIFICANCE AND IMPACT OF THE STUDY: Mathematical models used to predict microbial growth are based on liquid cultures data. Models describing growth on solid surfaces, highlighting the differences with liquids cultures, are scarce. In this work, we have demonstrated that a single Gompertz equation describes accurately the increase of the yeast colonies, up to the point where they reach their maximum size. The model can be used to quantify the differences in growth kinetics between solid and liquid media. Moreover, as all its parameters have biological meaning, it could be used to build secondary models predicting yeast growth on solid surfaces under several environmental conditions.


Assuntos
Debaryomyces/crescimento & desenvolvimento , Modelos Biológicos , Rhodotorula/crescimento & desenvolvimento , Saccharomyces cerevisiae/crescimento & desenvolvimento , Zygosaccharomyces/crescimento & desenvolvimento , Meios de Cultura , Cinética , Viabilidade Microbiana
2.
Opt Express ; 18(2): 799-808, 2010 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-20173902

RESUMO

The recent results reported in reference 1 have produced an increased interest in explaining deviations from the ideal behavior of the energetic variation of the diffraction efficiency of holographic gratings. This ideal behavior occurs when uniform gratings are recorded, and the index modulation is proportional to the energetic exposure. As a result, a typical sin(2) curve is obtained reaching a maximum diffraction efficiency and saturation at or below this value. However, linear deviations are experimentally observed when the first maximum on the curve is lower than the second. This effect does not correspond to overmodulation and recently in PVA/acrylamide photopolymers of high thickness it has been explained by the dye concentration in the layer and the resulting molecular weight of the polymer chains generated in the polymerization process. In this work, new insights into these deviations are gained from the analysis of the non-uniform gratings recorded. Therefore, we show that deviations from the linear response can be explained by taking into account the energetic evolution of the index modulation as well as the fringe bending in the grating.


Assuntos
Holografia/instrumentação , Armazenamento e Recuperação da Informação/métodos , Modelos Teóricos , Dispositivos Ópticos , Polímeros/química , Polímeros/efeitos da radiação , Refratometria/instrumentação , Simulação por Computador , Desenho Assistido por Computador , Desenho de Equipamento , Análise de Falha de Equipamento , Luz
3.
Phys Rev E Stat Nonlin Soft Matter Phys ; 76(1 Pt 2): 016608, 2007 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-17677586

RESUMO

The aim of this work was to perform a complete study of the dynamic and steady-state photoinduced processes of thick bacteriorhodopsin (bR) films, taking into account all the physical parameters and the coupling of rate equations with the energy transfer equation. The theoretical approach was compared with experimental data, and good concordance was found between both sets of data. The theoretical approach shows that the values of the rate constants for solid bR films are about two or three orders of magnitude lower than those observed in solution. It can also be noted that the temperature change during the experiment had a great influence on the final values of transmittance and, consequently, on the inhomogeneous distribution along the coordinate of light propagation. The study shows that, depending on the intensity and wavelength of the pump beam, we can obtain a very inhomogeneous profile of the population densities, which implies an inhomogeneous profile of the birefringence and dichroism. Therefore, this must be taken into account in the applications described for this system.

5.
Biochim Biophys Acta ; 544(3): 529-39, 1978 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-215226

RESUMO

32P phosphorylation of plasma membranes from human blood platelets, under conditions that closely resemble physiological ones (endogeneous phosphate donors and intact platelets in homologous plasma), result in the incorporation of the label mainly in a membrane glycoprotein of apparently high molecular weight (greater than 400 000). Dibutyryl cyclic AMP, an inhibitor of platelet aggregation, specifically increases the degree of phosphorylation of this glycoprotein. Moreover, it has been found that prostaglandin E1 one of the most potent inhibitors of platelet aggregation which also increases phosphorylation of the same glycoprotein, is significantly more effective than cyclic AMP. Cyclic GMP does not have any apparent effect on platelet aggregation. However, incubation of platelet-rich plasma with both cyclic GMP and cyclic AMP results in a partial recovery of the platelet responsiveness towards ADP-induced aggregation. Coincidently, the degree of phosphorylation of the high molecular weight glycoprotein under these conditions, although still higher than in controls (no nucleotides added), is significantly decreased as compared with cyclic AMP-treated cells. Furthermore, cyclic GMP inhibits the cyclic AMP-dependent protein kinase activity in isolated platelet plasma membranes. These results suggest a central role for this membrane phosphoglycoprotein in the triggering of platelet aggregation and, furthermore, suggest that modulation of its degree of phosphorylation may be exerted through some cyclic AMP/cyclic GMP relationship, which in the basal state might be critical for platelet responsiveness.


Assuntos
Plaquetas/metabolismo , AMP Cíclico/farmacologia , GMP Cíclico/farmacologia , Proteínas de Membrana/sangue , Membrana Celular/metabolismo , Glicoproteínas/metabolismo , Humanos , Proteínas de Membrana/metabolismo , Peso Molecular , Fosforilação , Agregação Plaquetária/efeitos dos fármacos , Prostaglandinas E/farmacologia , Proteínas Quinases/metabolismo
6.
Biochim Biophys Acta ; 982(2): 303-6, 1989 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-2752030

RESUMO

Amphotericin B (AmB) was shown to induce a Ca2+ influx across ergosterol- and cholesterol-containing large unilamellar liposomes, by following spectrophotometrically the formation of the Arsenazo III-Ca2+ complex. At equivalent antibiotic concentrations the Ca2+ influx was much more extensive through ergosterol-containing membranes (almost 100% with 1 microM AmB, 160 microM lipid) than through cholesterol-containing membranes (below 0.5 microM the influx of Ca2+ was negligible). In the presence of ergosterol-containing membranes the initial rate of Ca2+ influx had the same linear dependence on the ratio antibiotic/lipid whatever the lipid concentration, which was not the case in cholesterol-containing membranes. These results suggest that the channels responsible for the AmB-induced Ca2+ permeability across cholesterol- and ergosterol-containing liposomes have different structures.


Assuntos
Anfotericina B/farmacologia , Cálcio/metabolismo , Colesterol/farmacologia , Ergosterol/farmacologia , Ionóforos/farmacologia , Lipossomos/metabolismo , Permeabilidade da Membrana Celular
7.
Biochim Biophys Acta ; 455(2): 371-82, 1976 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-11835

RESUMO

Incubation of platelet-rich plasma with 32Pi leads to cellular uptake of the isotope and covalent incorporation into several cell constituents. Plasma membranes isolated from intact labelled platelets, delipidated and solubilized in sodium dodecyl sulfate, show, upon gel electorphoretic analysis, three main peaks of radioactivity: two in the molecular weight range 100 000-30 000 and an additional very slow migrating component strong positive by the peirodic acid-Schiff reaction. Treatment of the cells with dibutyryl cyclic AMP under conditions just sufficient to completely inhibit platelet aggregation leads to an increased isotope incorporation. Electrophoretic analysis of membranes isolated from dibutyryl cyclic AMP-treated cell reveals: (a) no change in the general pattern of distribution of the isotope, (b) no difference in the isotope incorporation to the two components of lower mol. wt. and (c) a marked increase (greater than 100%) in isotope incorporation in the slow migrating material as compared to membranes isolated from control cells. This material can be extracted from platelet plasma membranes after treatment of the membranes for 5 h with Triton X-100, at a detergent-to-protein ratio of 7.5. When the membrane material extracted with Triton X-100 is subjected to gel chromatography in Agarose (Biogel A-15m), the phosphorylated material that corresponds to the slow migrating band in polyacrylanide gel electrophoresis is eluted with or very close to the void volume of the column. Isoelectric focussing of this fraction, shows a single radioactive peak corresponding to an isolectric point of 3.78. The isolated component is pronase-sensitive, contains 52% of carbohydrate and 15% sialic acid. Analysis of the stability of the bound phosphate suggests that about 43% of it is bound as acyl-phosphate. The results reported, obtained through an approach that closely resembles physiological conditions are compatible with the participation of this membrane phosphoglycoprotein in the phenomena of platelet aggregation.


Assuntos
Plaquetas/metabolismo , Bucladesina/farmacologia , Membrana Celular/metabolismo , Fosfoproteínas/sangue , Plaquetas/efeitos dos fármacos , Eletroforese das Proteínas Sanguíneas , Membrana Celular/efeitos dos fármacos , Eletroforese em Gel de Poliacrilamida , Humanos , Concentração de Íons de Hidrogênio , Cinética , Peso Molecular
8.
J Immunol Methods ; 132(1): 63-71, 1990 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-2202766

RESUMO

We have developed a novel method that greatly simplifies the preparation of solid-phase HIV-1 envelope glycoproteins for use in an ELISA that detects serum antibodies to HIV envelope antigens. This method utilizes concanavalin A absorbed to wells of microtiter plates to affinity immobilize detergent-solubilized viral glycoproteins released in culture fluids of HIV-1 infected cell lines grown in serum free medium. Antibodies binding to ConA-immobilized viral antigens are detected by peroxidase-conjugated antibodies and appropriate enzyme substrates. Unlike most commercial HIV ELISAs, which utilize gp120 depleted-purified virus as the source of antigens and thus favor detection of antibodies to core antigens, the ConA envELISA is highly sensitive for detecting antibodies to native gp120, as evidenced by the strong reactivity of gp120-specific human monoclonal antibodies. Our results also suggest that representation of gp41 in the assay varies and depends on which virus infected cell lines are used for antigen production. Since this assay accurately identified 14 HIV-1 antibody positive patient sera and no false positives were detected among 16 HIV-1 negative sera, the ConA envELISA shows promise as an inexpensive assay for the serologic diagnosis of HIV infections.


Assuntos
Anticorpos Monoclonais/análise , Concanavalina A , Ensaio de Imunoadsorção Enzimática/métodos , Produtos do Gene env/imunologia , Anticorpos Anti-HIV/análise , HIV-1/imunologia , Animais , Western Blotting , Humanos , Camundongos , Camundongos Endogâmicos BALB C
9.
Opt Express ; 12(8): 1780-7, 2004 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-19475006

RESUMO

In this paper, we describe a photopolymerizable silica glass based on acrylamide (AA) and N,N'-methylenebisacrylamide (BMA) as monomers, triethanolamine (TEA) as coinitiator and yellowish eosin (YE) as photoinitiator. We studied different compositions, analyzing the diffraction efficiency, energetic exposure and effective thickness obtained in the holographic gratings. A diffraction efficiency of 60 % with an energetic exposure of 139 mJ/cm(2) and an effective thickness of 1.1 mm were obtained. Also, by varying the photopolymerizable composition of the material diffraction efficiencies higher than 80 % can be reached with an energetic exposure of 10 mJ/cm(2) and an effective thickness of 113 microm. These values are similar to those obtained in conventional photopolymer systems in polyvinylalcohol and better than the values reached in other sol-gel compositions. Also, 9 holograms were angular multiplexed with diffraction efficiencies between 6 and 12 % and total exposure time shorter than 150 ms, with a dynamic range M/#= 2.4.

10.
Opt Express ; 11(25): 3438-44, 2003 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-19471476

RESUMO

The holographic parameters of purple membrane-polyacrylamide films obtained from a mutant form of Halobacterium salinarum (originally Halobacterium halobium) were measured. The synthesized films have an absorption of around 2.5 at 532 nm and a pH of 8.65. The results show that diffraction efficiencies of about 1.2 % (measured at 633 nm) can be achieved with writing intensities in the range of 200-400 mW/cm2 (532 nm), and these values remain constant after saturation. Pump-probe experiments were also used to measure the M state lifetime and our PM films were found to have the lowest M state lifetime described at this pH.

11.
J La State Med Soc ; 142(12): 35-7, 1990 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-2277228

RESUMO

Systemic infections caused by saprophytic fungi are being diagnosed more frequently. We describe the second reported instance of Paecilomyces lilacinus causing infection in an immunocompromised host. The diagnosis and treatment of this unusual pathogen are discussed.


Assuntos
Leucemia Linfoide/complicações , Leucemia Mieloide Aguda/complicações , Micoses/etiologia , Paecilomyces , Criança , Feminino , Humanos , Tolerância Imunológica
12.
Neuroscience ; 160(1): 212-26, 2009 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-19409209

RESUMO

The rat facial whiskers form a high-resolution sensory apparatus for tactile information coding and are used by these animals for the exploration and perception of their environment. Previous work on the rat vibrissae system obtained evidence for vibration-based feature extraction by the whiskers, texture classification by the cortical neurons, and "low-pass", "high-pass", and "band-pass" filtering properties in both thalamic and cortical neurons. However, no data are available for frequency-dependent information processing in the brainstem sensory trigeminal complex (STC), the first relay station of the vibrissae pathway. In the present paper, we studied the frequency-dependent processing characteristics of the STC nuclei that mainly project to the thalamus, nuclei principalis, and interpolaris. This is the first time that STC nuclei have been studied together via a wide range of stimulation frequencies (1-40 Hz), four different and complementary metrics, and the same experimental protocol. Moreover, the role of corticofugal projection to these nuclei as well as the influence of input from the whiskers has been analyzed. We show that both nuclei perform frequency-dependent coding of tactile information: low pass and band-pass filtering occurs for the spiking rate in short post-stimuli time intervals, high-pass and band-pass filtering occurs for the spiking rate in long trains of stimuli, and an increase of response latencies and low pass filtering occurs for phase-locked stimuli. These information-processing characteristics are neither imposed by the sensorimotor cortex nor introduced by the afferent fibres. The sensorimotor cortex exerts a distinct modulatory effect on each nucleus.


Assuntos
Tronco Encefálico/fisiologia , Córtex Cerebral/fisiologia , Neurônios/fisiologia , Vibrissas , Potenciais de Ação , Análise de Variância , Animais , Feminino , Masculino , Microeletrodos , Vias Neurais/fisiologia , Estimulação Física , Ratos , Ratos Wistar , Tempo de Reação , Tálamo/fisiologia
13.
Opt Lett ; 31(15): 2317-9, 2006 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-16832471

RESUMO

The recording of holographic reflection gratings with a spatial frequency higher than 5400 lines/mm in photopolymerizable solgel materials is experimentally demonstrated. Diffraction efficiencies near 60% and a FWHM of 2.5 nm centered at 531.5 nm are achieved. Moreover, the effect of the energetic exposure is characterized at different recording intensities.

14.
Biol Cybern ; 76(5): 375-82, 1997 May.
Artigo em Inglês | MEDLINE | ID: mdl-9237363

RESUMO

Specialization is a common feature in animal societies that leads to an improvement in the fitness of the team members and to an increase in the resources obtained by the team. In this paper we propose a simple reinforcement learning approach to specialization in an artificial multi-agent system. The system is composed of homogeneous and non-communicating agents. Because there is no communication, the number of agents in the team can easily scale up. Agents have the same initial functionalities, but they learn to specialize and so cooperate to achieve a complex gathering task efficiently. Simulation experiments show how the multi-agent system specializes appropriately so as to reach optimal (or near-to-optimal) performance in unknown and changing environments.


Assuntos
Comunicação Animal , Comportamento Animal/fisiologia , Aprendizagem/fisiologia , Modelos Biológicos , Animais , Planejamento Ambiental
15.
Biol Cybern ; 78(3): 197-205, 1998 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9602523

RESUMO

Optimization of performance in collective systems often requires altruism. The emergence and stabilization of altruistic behaviors are difficult to achieve because the agents incur a cost when behaving altruistically. In this paper, we propose a biologically inspired strategy to learn stable altruistic behaviors in artificial multi-agent systems, namely reciprocal altruism. This strategy in conjunction with learning capabilities make altruistic agents cooperate only between themselves, thus preventing their exploitation by selfish agents, if future benefits are greater than the current cost of altruistic acts. Our multi-agent system is made up of agents with a behavior-based architecture. Agents learn the most suitable cooperative strategy for different environments by means of a reinforcement learning algorithm. Each agent receives a reinforcement signal that only measures its individual performance. Simulation results show how the multi-agent system learns stable altruistic behaviors, so achieving optimal (or near-to-optimal) performances in unknown and changing environments.


Assuntos
Altruísmo , Cibernética , Aprendizagem , Inteligência Artificial , Simulação por Computador , Comportamento Cooperativo , Humanos , Modelos Psicológicos , Reforço Psicológico
16.
Am J Pediatr Hematol Oncol ; 12(3): 319-24, 1990.
Artigo em Inglês | MEDLINE | ID: mdl-2240479

RESUMO

We report the first know case of disseminated fungal infection due to Fusarium proliferatum in a bone marrow transplant recipient to our knowledge. Fusarium was cultured from the blood, a paranasal sinus, and necrotic skin lesions. The isolate was sensitive to amphotericin B and on further sensitivity testing, synergy was demonstrated using rifampin in combination with amphotericin B. The patient had this infection while she was receiving alternate-day amphotericin, rifampin, and 5-flucytosine (5-FC) therapy. The infection was documented within 48 h of discontinuing daily granulocyte transfusions, which she had received for 3 weeks. The 5-FC was discontinued when sensitivities showed the organism resistant. After 6 weeks of treatment she showed complete remission of the infection, although neutrophil counts remained below 0.25 X 10(9)/L. From this case and from a review of the literature, it appears that synergic antifungal agents combined with leukocyte transfusions may be beneficial in the successful treatment of fusariosis in the compromised host.


Assuntos
Anfotericina B/uso terapêutico , Fusarium , Micoses/tratamento farmacológico , Infecções Oportunistas/tratamento farmacológico , Rifampina/uso terapêutico , Anfotericina B/administração & dosagem , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Aspergilose/complicações , Transplante de Medula Óssea/efeitos adversos , Pré-Escolar , Terapia Combinada , Feminino , Fusarium/isolamento & purificação , Humanos , Micoses/etiologia , Neutropenia/complicações , Infecções Oportunistas/etiologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/complicações , Leucemia-Linfoma Linfoblástico de Células Precursoras/imunologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapia , Pele/microbiologia , Picada de Aranha/complicações , Infecções Estafilocócicas/complicações
17.
J Theor Biol ; 215(2): 201-13, 2002 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-12051974

RESUMO

A proliferating population of cells may be considered complex when its proliferative or growth fraction P is lower than 1 and/or when it is formed by subpopulations with different mean cycle times. The present paper shows that in such complex populations exponential growth is consistent with a steady-state distribution of cells. Obviously, when P=1 then cell distribution is only a function of cell age. An analytical model has been developed to study complex populations including both quiescent fractions formed by cells with unreplicated genome (G(0) cells) and cells with fully duplicated chromosomes (Q(2) cells). The model also considers those quasi-quiescent cells in their last transit through G(1) and S (Q(1) and Q(s) cells) before becoming quiescent. In order to solve the difficulties of a direct analysis of the whole population, its kinetic parameters have been obtained by studying the negative exponential distribution of two subpopulations: one formed by the proliferating cells and another formed by the quasi-quiescent cells. Additionally, the model could be applied when quiescence is initiated at any other cycle phase different from G(1) and G(2), for instance, cells in the process of replicating their DNA or being at any other mitotic phases. The utility of the method was illustrated in populations which constitute the root meristems of both Allium cepa L. and Pisum sativum L. Three facts should be stressed: (1) the method seems to be rather powerful because it can be carried out from different sets of experimentally measured parameters; (2) the rate of division and, therefore, the population doubling time can be easily estimated by this method; and (3) it also allows the determination of the amount of cells that had become quiescent either before they had replicated their DNA (G(0)) or after having completed their replication (Q(2)), as well as those quasi-quiescent cells which are progressing throughout their last pre-replicative and replicative periods (thus Q(1) and Q(s), respectively).


Assuntos
Meristema/citologia , Raízes de Plantas/citologia , Allium , Ciclo Celular/fisiologia , Divisão Celular/fisiologia , Cinética , Modelos Biológicos
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