RESUMO
AIM: Asymptomatic atrial fibrillation (AF) detection and pulmonary veins isolation (PVI) outcome prediction remain challenging. Our aim was to study the association between apelin and paroxysmal AF in patients undergoing radiofrequency catheter PVI. METHODS: Sixty-three consecutive patients (55 ± 8years, 12 females) with paroxysmal AF without a structural heart disease and implanted ECG loop recorders undergoing PVI and healthy control group of 34 persons (41 ± 9.5years, 21 females) were included. Apelin plasmatic concentrations were measured before and three months after PVI. AF burden was continually assessed for three years. RESULTS: Apelin was significantly decreased in AF patients compared to the healthy controls (0.79 ± 0.09 vs 0.98 ± 0.06 ng/ml; p < 0.00001). Apelin plasmatic concentration of 0.89 ng/ml had 94 % specificity and 89â % sensitivity for AF prediction with the area under the curve (AUC) of 0.96. After propensity matching to sex, age and comorbidities, apelin concentration was significantly lower in AF group (0.78 ± 0.1 vs 0.99 ±0.06 ng/ml; p < 0.0001; AUC: 0.97). There was a significant inverse correlation between apelin concentration and AF burden both before and after PVI (Rho = â0.22; p = 0.05) and (Rho = â0.51; p = 0.006), respectively. There was no significant association between pre-PVI apelin and PVI long-term outcome. CONCLUSION: In patients without a structural heart disease apelin showed a significant specificity and sensitivity for AF prediction and inversely correlated with AF burden (Tab. 3, Fig. 3, Ref. 34).
Assuntos
Fibrilação Atrial , Ablação por Cateter , Veias Pulmonares , Apelina , Fibrilação Atrial/diagnóstico , Fibrilação Atrial/cirurgia , Feminino , Humanos , Recidiva , Resultado do TratamentoRESUMO
BACKGROUND: Previous studies showed an association between apelin and atrial fibrillation (AF). The aim of this study was to analyse the effect of pulmonary vein isolation (PVI) in patients with paroxysmal AF on plasmatic apelin concentrations. METHODS: Nine consecutive patients (aged from 43 to 69 years, 3 females and 6 males) with documented paroxysmal atrial fibrillation and implanted loop recorders (ILR) for continuous ECG monitoring were included in this study. All the patients underwent a radiofrequency catheter ablation with PVI. RESULTS: The plasmatic concentration of apelin increased after PVI. The average plasmatic concentration of apelin before PVI was 0.299 ng/ml (±0.16), 3 months after PVI 0.462 ng/ml (±0.10) and 9 months after PVI 0.565 ng/ml (±0.146). There was an increase in the concentration of apelin 3 months and 9 months after the PVI by 0.163 ng/ml (p=0.07) and by 0.266 ng/ml (p=0.01), respectively. The concentration of apelin inversely correlated with the AF burden (r=-0.44, p=0.03). CONCLUSIONS: Our study showed a significant increase in apelin levels after the reduction of AF burden via PVI and an inverse correlation with AF burden. Apelin might be a promising marker of AF (Tab. 2, Fig. 2, Ref. 28).
Assuntos
Apelina , Fibrilação Atrial , Biomarcadores , Ablação por Cateter , Adulto , Idoso , Apelina/sangue , Fibrilação Atrial/sangue , Fibrilação Atrial/diagnóstico , Fibrilação Atrial/cirurgia , Biomarcadores/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Veias Pulmonares , Recidiva , Resultado do TratamentoRESUMO
INTRODUCTION: The current paradigm claims a link between oxidative stress and atrial fibrillation. The aim of our research was to study a relation between the percentage of time spent in atrial fibrillation (AF burden) and concentrations of oxidative stress biomarkers, before and after pulmonary veins isolation (PVI). METHODOLOGY: We included 19 patients (mean age 55±10years, 4 females and 15 males) with implanted loop recorders undergoing PVI. Plasmatic concentrations of advanced glycation end-products (AGEs), fructosamine, advanced oxidation protein products and thiobarbituric-acid reacting substances (TBARS) were measured and AF burden was recorded immediately before and 3months after the PVI. AF burden was also recorded 9months after the PVI. RESULTS: Post procedural AGEs concentration significantly negatively correlated with AF burden after 3months (ρ=-0.63; p<0.01) and 9months (ρ=-0.5; p=0.04), respectively as well as TBARS concentration significantly negatively correlated with AF burden after 9months (ρ=-0.61; p=0.01). CONCLUSION: Our study showed AGEs and TBARS to be potential predictors for AF burden after the PVI. We suppose that the more oxidative stress after the PVI is provoked, the more fibrotic tissue is produced. That means a better electrical isolation of pulmonary veins and consequently a lower AF burden.
Assuntos
Fibrilação Atrial/sangue , Fibrilação Atrial/cirurgia , Produtos Finais de Glicação Avançada/sangue , Sistema de Condução Cardíaco/cirurgia , Veias Pulmonares/cirurgia , Espécies Reativas de Oxigênio/sangue , Substâncias Reativas com Ácido Tiobarbitúrico/análise , Fibrilação Atrial/diagnóstico , Biomarcadores/sangue , Ablação por Cateter , Diagnóstico por Computador/métodos , Eletrocardiografia , Feminino , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Estresse Oxidativo , Prognóstico , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Resultado do TratamentoRESUMO
Capillary electrochromatography (CEC) using polymer-based monolithic stationary phase has been developed as a promising method for the determination of lignans of Schisandra chinensis. The columns were prepared by in situ copolymerisation of acrylamide, N,N'-methylenebisacrylamide, vinylsulfonic acid and lauryl acrylate in presence of poly(ethylene glycol) as a porogenic agent. The columns [33 cm (24.5 cm effective length) x 75 microm I.D.] were successfully used to analyse and quantify the major lignans in extract of the seeds of Schisandra chinensis. Good separations were achieved in less than 35 min. The calibration graphs were linear in the range 0.025-1.0 mg/ml of given lignan with correlation coefficients between 0.9951 and 0.9996. The inter-day reproducibility of the peak area were below 3.9% and the inter-day reproducibility of the migration time were below 4.2%. The results of quantitative CEC analyses were compared with those obtained by reversed-phase HPLC, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in a good agreement with those determined by HPLC.
Assuntos
Resinas Acrílicas , Cromatografia Capilar Eletrocinética Micelar/métodos , Lignanas/análise , Magnoliopsida/embriologia , Sementes/química , Cromatografia Líquida de Alta Pressão , Cromatografia Capilar Eletrocinética Micelar/instrumentação , Reprodutibilidade dos TestesRESUMO
Within two vegetation periods, melissas from 17 European regions and the inland cultivar Citra were examined. Melissas differed in height, foliage, and essential oil content. In the cultivating conditions under study, two collections were possible in one year. In both experimental years at the stage just before blooming, or at the onset of blooming, plants 60-80 cm in height prevailed in the set. The share of leaves in the tops was 35-70%. In the second repeated harvest, plants with newly formed sprouts reached a height of 15-35 cm and had the best foliage (60-95%). The essential oil content in the leaves at the stage just prior to blooming, or at the onset of blooming, ranged from 0.06 to 0.16% (V/m), and the maximal essential oil content was in the plants from the second harvest (0.09-0.45%). The cultivar Citra was characterized by medium height (75-80 cm), stable values of foliage (1st harvest 55-60%, 2nd harvest 70-80%), and essential oil content (1st harvest 0.13%, 2nd harvest 0.23-0.27%).
Assuntos
Lamiaceae/química , Óleos Voláteis/análise , Plantas Medicinais/química , República Tcheca , Europa (Continente) , Lamiaceae/crescimento & desenvolvimento , Estruturas Vegetais/química , Plantas Medicinais/crescimento & desenvolvimentoRESUMO
The author described a modification of a simple undemanding method of staining Durcupan section of tissues fixed in aldehydes and in osmium. Methylene blue with azure II was used for the staining. After rinsing in water the sections were counterstained with basic fuchsin, or with saphranin O. Basophil structures are stained blue or even violet, fat and erythrocytes blue-green. After counterstaining with saphranin, mucus, collagen, and glycogen come out pink, or red case fuschin is used. Elastic tissue come out violet. The colours are bright, full of contrast, and practically fast.
Assuntos
Resinas Epóxi , Técnicas Histológicas , Microscopia Eletrônica/métodos , Coloração e Rotulagem/métodosRESUMO
In two cases of trichocellular leukaemia, specimens of blood, bone marrow and the spleen were evaluated not only by means of SEM and TEM but also with a view to their phagocytic and immunological properties. While immunological investigation rather suggested a B lymphocytic aetiology of the process, phagocytosis of Ferrocide (not, however, of latex) seemed to justify, in one case, also histoendothelial aetiology.
Assuntos
Leucemia de Células Pilosas/ultraestrutura , Adulto , Linfócitos B/ultraestrutura , Células Sanguíneas/ultraestrutura , Medula Óssea/ultraestrutura , Humanos , Leucemia de Células Pilosas/imunologia , Masculino , Pessoa de Meia-Idade , Fagocitose , Baço/ultraestruturaRESUMO
Infrared laser ablation (IRLA) was studied as a sample-introduction technique for the analysis of steels by inductively coupled plasma atomic emission spectrometry (ICP-AES). A comparison of two IRLA-ICP-AES systems based on Q-switched nanosecond Nd: YAG lasers was performed. The beam of the LINA-Spark atomizer (LSA Sarl, Cully, Switzerland) based on the Surelite 1-20 laser (Continuum, USA) was moved along a circle. A Perkin-Elmer Optima 3000 DV ICP system was used both with lateral and axial viewing modes. A laboratory-made ablation system based on the Brilliant laser (Quantel) was coupled to a Jobin-Yvon 170 Ultrace ICP (lateral viewing, polychromator part employed). A sample was rotated along a circle during ablation. Linearity of calibration plots was verified at least up to 19% Cr and 12% Ni without internal standardization for both LA-ICP-AES systems. Other elements examined were Mo up to 3%, Mn up 1.5%, Si up to 1.7%, and Cu up to 0.15%. The reproducibility was in the range 5-1 %RSD for a mass percentage 0.5-20% of steel constituents. The relative uncertainty of the centroids of the calibration lines was in the range from +/- 4% to +/- 12% for Cr, Ni, Mn, Mo, and Si, and from +/- 8% to +/- 19% for Cu. The lowest determinable quantities were calculated for calibration dependencies. Performances of both the IR-LA-ICP-AES were comparable.
RESUMO
Potential of infrared laser ablation (LA) coupled with ICP-AES as a technique suitable for the determination of trace elements (Zn, Cu, Ni, Cr, and V) in agricultural soils was studied. Operating parameters such as laser beam energy, laser beam focusing with respect to the sample surface, and velocity of the sample translation in the plane perpendicular to the laser beam were optimized. Soil samples were mixed with powdered Ag as a binder, and an internal standard (GeO(2)), and pressed into pellets. Calibration samples were prepared by adding known amounts of oxides of elements of interest into soils of known elemental composition and then processed in the same way as the analyzed samples. Calibration curves were found to be linear at least up to several hundreds of mg kg(-1) for the elements of interest. The elemental contents obtained by using LA-ICP-AES were compared with those obtained by analysis using wet chemistry followed by ICP-AES with pneumatic nebulization (PN). The results were in good agreement. Accuracy was also tested using certified reference soils with a bias not exceeding 10% relative.