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Biochem Biophys Res Commun ; 560: 14-20, 2021 06 30.
Artigo em Inglês | MEDLINE | ID: mdl-33965785

RESUMO

Clock genes express circadian rhythms in most organs. These rhythms are organized throughout the whole body, regulated by the suprachiasmatic nucleus (SCN) in the brain. Disturbance of these clock gene expression rhythms is a risk factor for diseases such as obesity. In the present study, to explore the role of clock genes in developing diabetes, we examined the effect of streptozotocin (STZ)-induced high glucose on Period1 (Per1) gene expression rhythm in the liver and the olfactory bub (OB) in the brain. We found a drastic increase of Per1 expression in both tissues after STZ injection while blood glucose content was low. After a rapid expression peak, Per1 expression showed no rhythm. Associated with an increase of glucose content, behavior became arrhythmic. Finally, we succeeded in detecting an increase of Per1 expression in mice hair follicles on day 1 after STZ administration, before the onset of symptoms. These results show that elevated Per1 expression by STZ plays an important role in the aggravation of diabetes.


Assuntos
Diabetes Mellitus Experimental/metabolismo , Fígado/metabolismo , Bulbo Olfatório/metabolismo , Proteínas Circadianas Period/biossíntese , Animais , Glicemia/efeitos dos fármacos , Peso Corporal/efeitos dos fármacos , Diabetes Mellitus Experimental/genética , Diabetes Mellitus Experimental/fisiopatologia , Ingestão de Líquidos/efeitos dos fármacos , Expressão Gênica , Cabelo/efeitos dos fármacos , Cabelo/metabolismo , Locomoção , Camundongos Endogâmicos C57BL , Proteínas Circadianas Period/genética , Periodicidade , Estreptozocina
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