Assuntos
Neoplasias Duodenais/diagnóstico , Neoplasias Duodenais/patologia , Endoscopia do Sistema Digestório/métodos , Linfoma Folicular/diagnóstico , Linfoma Folicular/patologia , Imagem de Banda Estreita/métodos , Idoso , Antígenos CD20/análise , Biomarcadores Tumorais/análise , Humanos , Masculino , Estadiamento de Neoplasias , Neprilisina/análise , Proteínas Proto-Oncogênicas c-bcl-2/análiseAssuntos
Neoplasias Duodenais/complicações , Infecções por HIV/complicações , Linfoma Difuso de Grandes Células B/complicações , Idoso , Neoplasias Duodenais/tratamento farmacológico , Neoplasias Duodenais/patologia , Endoscopia do Sistema Digestório , Infecções por HIV/tratamento farmacológico , Humanos , Linfoma Difuso de Grandes Células B/tratamento farmacológico , Linfoma Difuso de Grandes Células B/patologia , MasculinoRESUMO
The type 1 glucose transporter (GLUT1) gene encodes an integral membrane glycoprotein responsible for facilitating transfer of glucose across plasma membrane and is rapidly activated by serum, growth factors, and by oncogenic transformation. To elucidate the molecular mechanisms of regulation of GLUT1 gene expression, we isolated and characterized the mouse GLUT1 gene. DNA elements regulating transcription of the gene were analyzed in transient expression assays after transfection of NIH/3T3 cells with a low background chloramphenicol acetyltransferase (CAT) vector system pSVOOCAT. We identified two enhancer elements; the first one is located 2.7 kilobases upstream of the cap site of the gene which contains the homologous sequences with two 12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs), a serum response element, a cyclic AMP-responsive element (CRE) and three GC boxes, and the second one is located in the second intron of the gene which contains the homologous sequences with two TREs and one CRE. With the promoter alone the transcription of the gene is activated by src, only slightly activated by ras and is not activated by serum and platelet-derived growth factor. When the gene is accompanied by one of these enhancers, the transcription is activated by all these stimuli.