RESUMO
Expression of several heat shock operons, mainly coding for small heat shock proteins, is under the control of ROSE (repression of heat shock gene expression) in various rhizobial species. This negatively cis-acting element confers temperature control by preventing expression at physiological temperatures. We provide evidence that ROSE-mediated regulation occurs at the post-transcriptional level. A detailed mutational analysis of ROSE(1)-hspA translationally fused to lacZ revealed that its highly conserved 3'-half is required for repression at normal temperatures (30 degrees C). The mRNA in this region is predicted to form an extended secondary structure that looks very similar in all 15 known ROSE elements. Nucleotides involved in base pairing are strongly conserved, whereas nucleotides in loop regions are more divergent. Base substitutions leading to derepression of the lacZ fusion at 30 degrees C exclusively resided in potential stem structures. Optimised base pairing by elimination of a bulged residue and by introduction of complementary nucleotides in internal loops resulted in ROSE elements that were tightly repressed not only at normal but also at heat shock temperatures. We propose a model in which the temperature-regulated secondary structure of ROSE mRNA influences heat shock gene expression by controlling ribosome access to the ribosome-binding site.
Assuntos
Proteínas de Bactérias , Regulação Bacteriana da Expressão Gênica , Proteínas de Choque Térmico/genética , Resposta ao Choque Térmico , Processamento Pós-Transcricional do RNA , RNA Bacteriano/fisiologia , Rhizobiaceae/genética , Região 3'-Flanqueadora , Pareamento de Bases , Sequência de Bases , Análise Mutacional de DNA , Inativação Gênica , Modelos Genéticos , Conformação de Ácido Nucleico , Óperon , RNA Bacteriano/química , RNA Mensageiro/química , RNA Mensageiro/fisiologia , Sequências Reguladoras de Ácido Nucleico , Rhizobiaceae/metabolismoRESUMO
Characterization of the heat shock response in Clostridium acetobutylicum has indicated that at least 15 proteins are induced by a temperature upshift from 30 to 42 degrees C. These so-called heat shock proteins include DnaK and GroEL, two highly conserved molecular chaperones. Several genes encoding heat shock proteins of C. acetobutylicum have been cloned and analysed. The dnaK operon includes the genes orfA (a heat shock gene with an unknown function), grpE, dnaK, and dnaJ; and the groE operon the genes groES and groEL. The hsp18 gene coding for a member of the small heat shock protein family constitutes a monocistronic operon. Interestingly, the heat shock response in this bacterium is regulated by a mechanism, which is obviously different from that found in Escherichia coli. So far, no evidence for a heat shock-specific sigma factor of the RNA polymerase in C. acetobutylicum has been found. In this bacterium, like in many Gram-positive and several Gram-negative bacteria, a conserved inverted repeat is located upstream of chaperone/chaperonin-encoding stress genes such as dnaK and groEL and may be implicated as a cis-acting regulatory site. The inverted repeat is not present in the promoter region of hsp18. Therefore, in C. acetobutylicum there are at least two classes of heat shock genes with respect to the type of regulation. Evidence has been found that a repressor is involved in the regulation of the heat shock response in C. acetobutylicum. However, this regulation seems to be independent of the inverted repeat motif, and the mechanism by which the inverted repeat motif mediates regulation remains to be elucidated.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Clostridium/genética , Regulação Bacteriana da Expressão Gênica , Resposta ao Choque Térmico/genética , Sequência de Bases , Dados de Sequência MolecularRESUMO
The rpoH3 gene of Bradyrhizobium japonicum codes for one of three sigma32-type transcription factors in this organism and is flanked by rag (rpoH3-associated) genes comprising the chromosomal arrangement ragABrpoH3ragCD. The first genes in this cluster code for a classical two-component regulatory system with an unknown function (Narberhaus et al., 1997. Mol. Microbiol. 24, 93-104). The deduced proteins of the last two genes display a high sequence similarity to heavy metal or multidrug efflux pumps of the RND (Resistance/Nodulation/cell Division)-MFP (Membrane Fusion Protein) family. Reverse transcription and polymerase chain reaction (RT-PCR) analysis demonstrated that ragC is cotranscribed with rpoH3. Mutant strains carrying disrupted rag genes or an extented deletion of the rag locus exhibited neither an apparent growth defect nor a deficiency in the symbiotic interaction with soybean roots. The minimal inhibitory concentrations (MICs) of various metals and organic compounds were identical for the wild-type and mutant strains. Moreover, translational lacZ fusions to each of the first four genes of the rag cluster showed a very low expression under all conditions tested; hence, the substrate for the putative efflux pump has not been uncovered.
Assuntos
Proteínas de Bactérias/genética , Bradyrhizobium/genética , Proteínas de Membrana/genética , Fatores de Transcrição/genética , Bradyrhizobium/metabolismo , Mapeamento Cromossômico , Expressão Gênica , Genes Bacterianos , Família Multigênica , Mutação , Fenótipo , Transcrição GênicaRESUMO
The proteolysis of regulatory proteins plays an important role in the control of gene expression. The Escherichia coli heat shock sigma factor RpoH (sigma(32)) is highly unstable. Its instability is determined by interactions with the DnaK chaperone machine, RNA polymerase and the ATP-dependent protease FtsH. Bradyrhizobium japonicum expresses three RpoH proteins of which RpoH(1) is highly stable. To determine which regions of E. coli RpoH determine protein lability, we generated a number of truncated versions and hybrid proteins. Truncation of N-terminal amino acids had no, and deletion of C-terminal amino acids only a minor effect on stability of RpoH. A major determinant of RpoH lability was mapped to a region of about 85 amino acids (residues 36-122) roughly comprising the sigma factor region 2. This is the first demonstration of an internal RpoH region being responsible for FtsH-mediated degradation.
Assuntos
Proteínas de Bactérias/metabolismo , Proteínas de Escherichia coli , Escherichia coli/metabolismo , Proteínas de Choque Térmico/química , Proteínas de Choque Térmico/metabolismo , Proteínas de Membrana/metabolismo , Fator sigma , Fatores de Transcrição/química , Fatores de Transcrição/metabolismo , Proteases Dependentes de ATP , Sequência de Aminoácidos , Bradyrhizobium/enzimologia , Proteínas de Choque Térmico HSP70/química , Proteínas de Choque Térmico HSP70/metabolismo , Dados de Sequência Molecular , Mutagênese , Plasmídeos/metabolismo , Ligação Proteica , Estrutura Terciária de Proteína , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/metabolismo , Homologia de Sequência de Aminoácidos , Fatores de Tempo , beta-Galactosidase/metabolismoRESUMO
The complete dnaJ gene of Clostridium acetobutylicum was isolated by chromosome walking using the previously cloned 5' end of the gene as a probe. Nucleotide sequencing of a positively reacting 2.2-kb HincII fragment, contained in the recombinant plasmid pKG4, revealed that the reading frame of the dnaJ gene of C. acetobutylicum consists of 1125 bp, encoding a protein of 374 amino acids with a calculated M(r) of 40376 and an isoelectric point of 9.54. The deduced amino acid sequence showed high similarity to the DnaJ proteins of other bacteria (e.g. Escherichia coli, Bacillus subtilis) as well as of an archaeon (Methanosarcina mazei) and to the corresponding proteins of eukaryotes (Saccharomyces cerevisiae, Homo sapiens). The areas of similarity included a conserved N-terminal domain of about 70 amino acids, a glycine-rich region of about 30 residues, and a central domain containing four repeats of a CXXCXGXG motif, whereas the C-terminal domain was less conserved. Northern (RNA) blot analysis indicated that dnaJ is induced by heat shock and that it is part of the dnaK operon of C. acetobutylicum. The 5' end (901 bp) of another gene (orfB), downstream of dnaJ and not heat-inducible, showed no significant similarity to other sequences available in EMBL and GenBank databases.
Assuntos
Clostridium/genética , Genes Bacterianos/genética , Proteínas de Choque Térmico/genética , Sequência de Aminoácidos , Bactérias/genética , Sequência de Bases , Clonagem Molecular , DNA Bacteriano/genética , Proteínas de Escherichia coli , Biblioteca Gênica , Proteínas de Choque Térmico HSP40 , Proteínas de Choque Térmico/química , Dados de Sequência Molecular , RNA Bacteriano/análise , RNA Mensageiro/análise , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Transcrição GênicaRESUMO
We identified by cloning and DNA sequence analysis the phosphate regulatory gene phoB of Bradyrhizobium japonicum. The deduced gene product displayed pronounced similarity to the PhoB protein of Sinorhizobium meliloti (71.4% identical amino acids). Escherichia coli (50.2%) and other bacterial species. Insertion of a kanamycin resistance cassette into phoB led to impaired growth of the B. japonicum mutant in media containing approximately 25 microM phosphate or less. A standard plant infection test using wild-type and phoB-defective B. japonicum strains showed that the phoB mutation had no effect on the symbiotic properties of B. japonicum with its soybean host plant.
Assuntos
Proteínas de Bactérias/genética , Genes Bacterianos , Fixação de Nitrogênio , Fosfatos/metabolismo , Rhizobium/genética , Sequência de Aminoácidos , Dados de Sequência Molecular , Mutação , Rhizobium/fisiologiaRESUMO
The expression of eubacterial heat shock genes is efficiently controlled at the transcriptional level by both positive and negative mechanisms. Positive control operates by the use of alternative sigma factors that target RNA polymerase to heat shock gene promoters. Alternatively, bacteria apply repressor-dependent mechanisms, in which transcription of heat shock genes is initiated from a classical housekeeping promoter and cis-acting DNA elements are used in concert with a cognate repressor protein to limit transcription under physiological conditions. Eight examples of negative regulation will be presented, among them the widespread CIRCE/HrcA system and the control by HspR in Streptomyces. Both mechanisms are designed to permit simple feedback control at the level of gene expression. Many bacteria have established sophisticated regulatory networks, often combining positive and negative mechanisms, in order to allow fine-tuned heat shock gene expression in an environmentally responsive way.
Assuntos
Regulação Bacteriana da Expressão Gênica , Proteínas de Choque Térmico/genéticaRESUMO
The groESL operon of Clostridium acetobutylicum was cloned in Escherichia coli by using a gene probe of E. coli groESL. Sequencing of a positively reacting 2.2-kbp HindIII fragment contained in the recombinant plasmid pFN1 and a 2.5-kbp XbaI fragment present in pFN4 revealed that both fragments partially overlapped and together spanned 3,493 bp of the clostridial chromosome. Two complete open reading frames (288 and 1632 bp) were found and identified as the groES- and groEL-homologous genes of C. acetobutylicum, respectively. The 3' end of a third gene (orfZ), which was divergently transcribed, showed no significant homology to other sequences available in the EMBL and GenBank data bases. The length of the groESL-specific mRNA (2.2 kb), a transcription terminator downstream of groEL, and a transcription start site upstream of groES, identified by primer extension analysis, indicated that groES and groEL of C. acetobutylicum are organized in a bicistronic operon. From the transcription start site, the promoter structure 5'-TTGCTA (17 bp) TATTAT that shows high homology to the consensus promoter sequence of gram-positive bacteria as well as E. coli was deduced. Transcription of the groESL operon was strongly heat inducible, and maximum levels of mRNA were detected 15 min after heat shock from 30 to 42 degrees C. An 11-bp inverted repeat, located between promoter and translation start sites of groES and partially identical with similar structures in front of several heat shock genes of other bacteria, may play an important role in the regulation of heat shock gene expression in this organism.
Assuntos
Clostridium/genética , Proteínas de Choque Térmico/genética , RNA Mensageiro/análise , Sequência de Aminoácidos , Proteínas de Bactérias/genética , Sequência de Bases , Chaperonina 10 , Chaperonina 60 , Clonagem Molecular , Sequência Consenso , Escherichia coli/genética , Regulação Bacteriana da Expressão Gênica , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Óperon/genética , Sequências Reguladoras de Ácido Nucleico/genética , Sequências Repetitivas de Ácido Nucleico/genética , Homologia de Sequência do Ácido Nucleico , Transcrição GênicaRESUMO
Rhizobia are the only bacteria known to induce a multitude of small heat shock proteins (sHsps) upon temperature upshift. The sHsps of Bradyrhizobium japonicum fall into two different classes, class A and class B. Here, we studied the chaperone activity and oligomeric features of two representative members of each class. The purified sHsps were efficient chaperones, as demonstrated by their ability to prevent thermally induced aggregation of citrate synthase in vitro. Homo-oligomer formation of all four sHsps was demonstrated by gel filtration and by two independent co-purification approaches. Mixed oligomers were readily observed between members of the same class, even when these proteins originated from different species such as Escherichia coli and B. japonicum. The chaperone activity of purified hetero-oligomers was indistinguishable from the activity of homo-oligomers. Heteromeric complexes were never obtained between class A and class B sHsps, indicating that hetero-oligomer formation is restricted to sHsps of the same class.
Assuntos
Bradyrhizobium/metabolismo , Proteínas de Escherichia coli , Proteínas de Choque Térmico/metabolismo , Chaperonas Moleculares/metabolismo , Cromatografia em Gel , Eletroforese em Gel de Poliacrilamida , Escherichia coli , Proteínas de Choque Térmico/isolamento & purificação , Corpos de Inclusão/metabolismo , PolímerosRESUMO
The response to heat stress was examined in Thermoanaerobacterium thermosulfurigenes EM1. Upon a temperature shift-up from 50 degrees to 62 degrees C, four heat shock proteins (hsps) were synthesized at an elevated level. Two proteins were found to be immunologically related to the Escherichia coli GroEL protein and the Mycobacterium tuberculosis hsp71 (DnaK similar protein), and the corresponding groE and dnaK homologous sequences were detected in the chromosome of T. thermosulfurigenes EM1. The heat shock response in this thermophile was transient, with a maximum synthesis of hsps between 10 and 15 min after the shock. The enhanced synthesis of DnaK and GroEL was consistent with increased mRNA levels of the genes, which reached a maximum 15 min after heat treatment.
Assuntos
Clostridium/metabolismo , Proteínas de Escherichia coli , Regulação Bacteriana da Expressão Gênica/genética , Proteínas de Choque Térmico HSP70 , Proteínas de Choque Térmico/biossíntese , Antígenos de Bactérias/análise , Antígenos de Bactérias/genética , Proteínas de Bactérias/análise , Proteínas de Bactérias/genética , Sequência de Bases , Chaperonina 60 , Clostridium/genética , DNA Bacteriano/análise , Genes Bacterianos/genética , Proteínas de Choque Térmico/análise , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/imunologia , Temperatura Alta , Dados de Sequência Molecular , RNA Bacteriano/análise , RNA Bacteriano/genética , RNA Mensageiro/análise , RNA Mensageiro/genéticaRESUMO
The dnaK gene region of Clostridium acetobutylicum was cloned in Escherichia coli by using the pBluescript SK+ and pUC18 vectors. By using the E. coli dnaK gene as a probe and by in vivo chromosome walking, three positive clones harboring the recombinant plasmids pKG1, pKG2, and pKG3 containing 1.2-kbp HindIII, 3.55-kbp EcoRV, and 1.2-kbp PstI fragments of the chromosome of C. acetobutylicum, respectively, were isolated. The cloned fragments partially overlapped, and together they spanned 4,083 bp of the clostridial genome that were completely sequenced. On one strand, four open reading frames of which the last was obviously truncated were identified. The last three genes showed high homology to the grpE, dnaK, and dnaJ heat shock genes of E. coli, respectively. They were preceded by an open reading frame (orfA) without any homology to sequences available in the EMBL or GenBank data bases. Typical translational start sites could be found in front of all four genes. Northern (RNA) blot analysis revealed transcripts of this region with a maximum length of 5.0 kb. Thus, these genes are probably organized in an operon. A transcription terminator could be found between the dnaK and dnaJ genes. By primer extension analysis, a major heat-inducible transcription start site was identified 49 bases upstream of orfA. This site was preceded by a region (5'-TTGACA[17 bp]TATTTT) that exhibited high homology to the consensus promoter sequences of gram-positive bacteria as well as sigma 70-dependent E. coli. Between this promoter and the initiation codon of orfA, a hairpin-loop structure with a possible regulatory role in the expression of these genes was found. Additional heat-inducible transcription start sites were located 69 bases upstream of orfA and 87 bases upstream of grpE; the corresponding promoter regions showed less similarity to other known promoter sequences. Maximum mRNA levels of this heat shock operon were found about 15 min after a heat shock from 30 to 42 degrees C. Our results indicate that orfA codes for an unknown heat shock protein.
Assuntos
Proteínas de Bactérias/genética , Clostridium/genética , Proteínas de Escherichia coli , Proteínas de Choque Térmico HSP70 , Proteínas de Choque Térmico/genética , Sequência de Aminoácidos , Sequência de Bases , Passeio de Cromossomo , Clonagem Molecular , Proteínas de Choque Térmico HSP40 , Dados de Sequência Molecular , Regiões Promotoras Genéticas/genética , RNA Mensageiro/análise , Fases de Leitura , Homologia de Sequência do Ácido Nucleico , Transcrição GênicaRESUMO
The Bradyrhizobium japonicum ftsH gene was cloned by using a set of widely applicable degenerated oligonucleotides. Western blot experiments indicated that the FtsH protein was produced under standard growth conditions and that it was not heat inducible. Attempts to delete the ftsH gene in B. japonicum failed, suggesting a pivotal cellular function of this gene. The expression of B. japonicum ftsH in an ftsH-negative Escherichia coli strain significantly enhanced the fitness of this mutant and reduced the steady-state level of sigma(32).
Assuntos
Proteínas de Bactérias/genética , Bradyrhizobium/genética , Regulação Bacteriana da Expressão Gênica , Proteínas de Membrana/genética , Proteases Dependentes de ATP , Sequência de Aminoácidos , Proteínas de Bactérias/biossíntese , Clonagem Molecular , Escherichia coli/metabolismo , Proteínas de Escherichia coli , Teste de Complementação Genética , Resposta ao Choque Térmico/genética , Proteínas de Membrana/biossíntese , Dados de Sequência Molecular , Mutação , Nitrogênio/metabolismo , Homologia de Sequência de Aminoácidos , Fatores de TempoRESUMO
Seven genes coding for small heat shock proteins (sHsps) in Bradyrhizobium japonicum have been identified. They are organized in five operons that are coordinately regulated by ROSE, a negatively cis-acting DNA element. The deduced sHsps can be divided into two separate classes: class A, consisting of proteins that show similarity to Escherichia coli IbpA and IbpB, and class B, whose members display significant similarity to other sHsps from prokaryotes and eukaryotes. Two-dimensional gel electrophoresis and Edman sequencing revealed the presence of at least 12 sHsps in B. japonicum, indicating a remarkable abundance of sHsps in this organism. Three additional members of class A and two potentially novel heat shock proteins were identified on the basis of their amino termini. The presence of multiple sHsps was also demonstrated for a variety of Rhizobium and Bradyrhizobium species by immunoblot analysis and two-dimensional gel electrophoresis. An extensive database survey revealed that, in contrast to the rhizobia, other bacteria contain maximally two sHsps whereas many plants have been reported to possess a sHsp superfamily.
Assuntos
Proteínas de Bactérias/genética , Genes Bacterianos , Proteínas de Choque Térmico/genética , Rhizobiaceae/genética , Sequência de Aminoácidos , Proteínas de Bactérias/classificação , Proteínas de Bactérias/isolamento & purificação , Sequência de Bases , Primers do DNA/genética , Eletroforese em Gel Bidimensional , Regulação Bacteriana da Expressão Gênica , Proteínas de Choque Térmico/classificação , Proteínas de Choque Térmico/isolamento & purificação , Temperatura Alta , Dados de Sequência Molecular , Óperon , Rhizobiaceae/química , Rhizobium/química , Rhizobium/genética , Homologia de Sequência de Aminoácidos , Especificidade da EspécieRESUMO
In the free-living diazotroph Klebsiella pneumoniae, the NifA protein is required for transcription of all nif (nitrogen fixation) operons except the regulatory nifLA operon itself. NifA activates transcription of nif operons by the alternative holoenzyme form of RNA polymerase, sigma 54 holoenzyme. In vivo, NifL is known to antagonize the action of NifA in the presence of molecular oxygen or combined nitrogen. We now demonstrate inhibition by NifL in vitro in both a coupled transcription-translation system and a purified transcription system. Crude cell extracts containing NifL inhibit NifA activity in the coupled system, as does NifL that has been solubilized with urea and allowed to refold. Inhibition is specific to NifA in that it does not affect activation by NtrC, a transcriptional activator homologous to NifA, or transcription by sigma 70 holoenzyme. Renatured NifL also inhibits transcriptional activation by a maltose-binding protein fusion to NifA in a purified transcription system, indicating that no protein factor other than NifL is required. Since inhibition in the purified system persists anaerobically, our NifL preparation does not sense molecular oxygen directly.
Assuntos
Transportadores de Cassetes de Ligação de ATP , Proteínas de Bactérias/efeitos dos fármacos , Proteínas de Bactérias/farmacologia , Proteínas de Escherichia coli , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Genes Bacterianos , Klebsiella pneumoniae/genética , Proteínas de Transporte de Monossacarídeos , Fixação de Nitrogênio/genética , Fatores de Transcrição/efeitos dos fármacos , Proteínas de Transporte/efeitos dos fármacos , Proteínas de Transporte/genética , Sistema Livre de Células , Escherichia coli/genética , Proteínas Ligantes de Maltose , Biossíntese de Proteínas/efeitos dos fármacos , Desnaturação Proteica , Proteínas Recombinantes/biossíntese , Transdução de Sinais , Transcrição Gênica/efeitos dos fármacosRESUMO
Expression of at least ten genes in Bradyrhizobium japonicum, seven of which code for small heat shock proteins (sHsps), is under the control of ROSE (repression of heat shock gene expression). This negatively cis-acting DNA element confers temperature control to a sigma(70)-type promoter. Here, we show that ROSE elements are not restricted to B. japonicum but are also present in Bradyrhizobium sp. (Parasponia), Rhizobium sp. strain NGR234 and Mesorhizobium loti. An overall alignment of all ROSE sequences reveals a highly conserved and probably functionally important region towards the 3'-end of the element. Moreover, we provide genetic evidence for the previously proposed presence of multiple sHsps in these organisms. Primer-extension data of five newly identified ROSE-associated operons show that transcription is repressed at low temperatures and induced after a temperature upshift. Translational ROSE-hsp'-'lacZ fusions of Bradyrhizobium sp. (Parasponia) and Rhizobium sp. strain NGR234 integrated into the chromosome of B. japonicum were heat-responsive. The functionality of these heterologous ROSE elements hints at a common regulatory principle conserved in various rhizobia.
Assuntos
Bradyrhizobium/genética , Regulação Bacteriana da Expressão Gênica , Genes Bacterianos/genética , Proteínas de Choque Térmico/genética , Elementos de Resposta/genética , Rhizobium/genética , Proteínas de Bactérias/genética , Sequência de Bases , Clonagem Molecular , Genótipo , Resposta ao Choque Térmico/genética , Dados de Sequência Molecular , Óperon/genética , Fenótipo , Alinhamento de Sequência , Especificidade da Espécie , Sítio de Iniciação de Transcrição , Transcrição Gênica/genética , beta-Galactosidase/genéticaRESUMO
The heat shock response of Bradyrhizobium japonicum is controlled by a complex network involving two known regulatory systems. While some heat shock genes are controlled by a highly conserved inverted-repeat structure (CIRCE), others depend on a sigma 32-type heat shock sigma factor. Using Western blot (immunoblot) analysis, we confirmed the presence of a sigma 32-like protein in B. japonicum and defined its induction pattern after heat shock. A B. japonicum rpoH-like gene (rpoH1) was cloned by complementation of an Escherichia coli strain lacking sigma 32. A knockout mutation in rpoH1 did not abolish sigma 32 production in B. japonicum, and the rpoH1 mutant showed the wild-type growth phenotype, suggesting the presence of multiple rpoH homologs in this bacterium. Further characterization of the rpoH1 gene region revealed that the rpoH1 gene is located in a heat shock gene cluster together with the previously characterized groESL1 operon and three genes encoding small heat shock proteins in the following arrangement: groES1, groEL1, hspA, rpoH1, hspB, and hspC. Three heat-inducible promoters are responsible for transcription of the six genes as three bicistronic operons. A sigma 32-dependent promoter has previously been described upstream of the groESL1 operon. Although the hspA-rpoH1 and hspBC operons were clearly heat inducible, they were preceded by sigma 70-like promoters. Interestingly, a stretch of about 100 bp between the transcription start site and the start codon of the first gene in each of these two operons was nearly identical, making it a candidate for a regulatory element potentially allowing heat shock induction of sigma 70-dependent promoters.
Assuntos
Regulação Bacteriana da Expressão Gênica , Genes Bacterianos , Proteínas de Choque Térmico/genética , Resposta ao Choque Térmico/genética , Família Multigênica , Rhizobiaceae/genética , Fator sigma/genética , Fatores de Transcrição , Sequência de Aminoácidos , Proteínas de Bactérias/genética , Sequência de Bases , Chaperoninas/genética , Clonagem Molecular , Teste de Complementação Genética , Temperatura Alta , Dados de Sequência Molecular , Mutação , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Transcrição GênicaRESUMO
Bradyrhizobium japonicum possesses a subclass of heat-shock genes whose members are transcribed from a sigma 32 consensus promoter. Having identified previously one gene (rpoH1) encoding a sigma 32-like RNA polymerase transcription factor, we report here the characterization of two additional rpoH-like genes (rpoH2 and rpoH3). B. japonicum thus represents the first example of an organism possessing an rpoH multigene family. All three rpoH genes encode functional proteins that are able to initiate transcription from the Escherichia coli groE promoter. Each rpoH gene is apparently regulated by a different mechanism. Although both rpoH1 and rpoH2 are transcribed from sigma 70-type promoters, transcription of the rpoH1 operon was found to be heat inducible by an unknown mechanism, whereas the level of rpoH2 mRNA decreased after heat shock. At extreme temperatures (48 degrees C), rpoH2 was transcribed from a second promoter that resembled the E. coli sigma E-type promoter. The rpoH3 gene was found to be associated with two upstream genes, ragA and ragB, coding for a classical two-component regulatory system. Transcription initiated from a promoter that mapped in front of the putative response regulator gene ragA, suggesting that ragA, ragB and rpoH3 are organized in an operon. The ragA promoter was similar to a sigma 32 consensus promoter. The three B. japonicum rpoH genes also varied in their significance to support growth of the organism. While the rpoH2 gene could not be eliminated by mutation, knock-out mutants of rpoH1 and/ or rpoH3 were readily obtained and shown to be indistinguishable from the wild type under aerobic growth conditions or during root-nodule symbiosis. We conclude that rpoH2 is essential for the synthesis of cellular proteins under physiological growth conditions, whereas rpoH1, and probably also rpoH3, are involved in their synthesis during the stress response.
Assuntos
Proteínas de Bactérias/genética , Genes Bacterianos , Proteínas de Choque Térmico/genética , Rhizobiaceae/genética , Fator sigma/genética , Fatores de Transcrição/genética , Sequência de Aminoácidos , Proteínas de Bactérias/metabolismo , Sequência de Bases , Proteínas de Choque Térmico/metabolismo , Dados de Sequência Molecular , Mutação , Filogenia , Rhizobiaceae/classificação , Rhizobiaceae/metabolismo , Homologia de Sequência de Aminoácidos , Fator sigma/metabolismo , Fatores de Transcrição/metabolismoRESUMO
The NIFA protein of Klebsiella pneumoniae is required for transcription of all nif (nitrogen fixation) operons except the regulatory nifLA operon itself. NIFA activates transcription of nif operons by the alternative holoenzyme form of RNA polymerase, sigma 54-holoenzyme, in a nucleoside triphosphate (NTP)-dependent manner. NIFL antagonizes the action of NIFA in the presence of molecular oxygen or combined nitrogen. The NIFA protein of K. pneumoniae is composed of three domains: an N-terminal domain with unclear function, a central catalytic domain, and a C-terminal DNA-binding domain. We report that the isolated central domain of NIFA activates transcription in vitro and that this activation requires NTP with a hydrolyzable beta-gamma bond, as does activation by intact NIFA. Transcriptional activation by the isolated central domain has the heat lability characteristic of intact NIFA and is inhibited by NIFL. The central domain has an NTPase activity that is also heat-labile but is not inhibited by NIFL. Taken together, these results imply that NIFL interferes with contact between NIFA and sigma 54-holoenzyme.
Assuntos
Proteínas de Bactérias/fisiologia , Regulação Bacteriana da Expressão Gênica , Genes Bacterianos , Klebsiella pneumoniae/genética , Fixação de Nitrogênio/genética , Fatores de Transcrição/fisiologia , Adenosina Trifosfatases/genética , Proteínas de Bactérias/antagonistas & inibidores , Sequência de Bases , Proteínas de Ligação a DNA/genética , Temperatura Alta , Dados de Sequência Molecular , Oligodesoxirribonucleotídeos/química , Fragmentos de Peptídeos , Regiões Promotoras Genéticas , RNA Mensageiro/genética , Fatores de Transcrição/antagonistas & inibidores , Transcrição GênicaRESUMO
The hspArpoH1 and hspBCdegP heat shock operons of Bradyrhizobium japonicum are preceded by a novel, conserved DNA element of approximately 100 bp, which is responsible for the temperature-regulated transcription of their sigma70-type promoters. We designated this motif ROSE for repression of heat shock gene expression and found additional ROSE elements upstream of two newly identified heat shock operons. A critical core region in the hspA-associated ROSE1 was defined by introducing insertions or deletions. While four mutants retained the ability to repress transcription of the hspArpoH1 operon, five deletion mutants produced elevated hspA mRNA levels under low-temperature growth conditions. Derepression was confirmed by increased RpoH1 levels in non-heat-shocked cells from one of these mutants and by strains that contained a translational hspA-lacZ fusion associated with mutated ROSE1 elements. The hspArpoH1 operon was efficiently transcribed in vitro, and a deletion of ROSE1 did not impair this activity. Gel retardation experiments demonstrated that a protein in non-heat-shocked cells specifically binds to the intact ROSE1 element but not to a mutated element lacking the core region. Taken together, these results indicate that a central region of ROSE serves as a binding site for a repressor protein under standard growth conditions in order to prevent the undesired transcription of heat shock genes.
Assuntos
Proteínas de Bactérias/genética , DNA Bacteriano/genética , Regulação Bacteriana da Expressão Gênica/genética , Genes Bacterianos/genética , Proteínas de Choque Térmico/genética , Transcrição Gênica , Proteínas de Bactérias/metabolismo , Sequência de Bases , Sítios de Ligação , DNA Bacteriano/química , Escherichia coli/genética , Proteínas de Choque Térmico/metabolismo , Dados de Sequência Molecular , Mutação/genética , Óperon/genética , Fatores de TempoRESUMO
A degP (htrA)-like gene of Bradyrhizobium japonicum was identified immediately downstream of two genes (hspB and hspC) coding for small heat-shock proteins. All three genes are oriented in the same direction and are separated by only 85 and 72 bp, and a heat-inducible transcript covering hspB, hspC, and degP was detected by RT-PCR. These results show that the genes are organized in an operon. Two mutants, a degP insertion mutant and a DeltahspBCdegP mutant, were constructed by marker replacement mutagenesis. Immunoblot analysis performed with a serum raised against the amino-terminal end of IbpA, an HspB homolog of Escherichia coli, identified three heat-inducible protein bands in B. japonicum extract, one of which was missing in the deletion mutant. None of the mutants showed an obvious defect during growth at different temperatures, after heat-shock treatment, or in the presence of solvents. Moreover, they were not affected in root-nodule symbiosis, indicating that the small heat-shock proteins HspB and HspC and the DegP homolog of B. japonicum are not required under a wide range of growth conditions.