Performance, egg quality, and blood plasma chemistry of laying hens fed hempseed and hempseed oil.

The aim of this study was to compare the performance of hens (feed intake, rate of lay, egg weight, and BW gain), egg quality and blood biochemistry (enzymes, electrolytes, proteins, and other plasma constituents) of laying hens fed diets containing hemp products. Forty-eight Lohmann LSL-Classic (white-egg layers; 19 wk of age) were individually caged and fed 1 of 6 wheat-barley-soybean-based diets for a period of 12 wk. The diets consisted of hempseed (HS; 10, 20, or 30%), hempseed oil (HO; 4.5 or 9.0%), or a control diet (corn oil-based). All diets were formulated to contain similar levels of crude fat (11%), energy (2,800 kcal/kg), and CP (17%). Data were analyzed as a completely randomized design using the repeated measure analysis of the PROC MIXED procedure of SAS. The results indicated that the inclusion of up to 30 and 9.0% HS and HO, respectively, to diets of laying hens had no significant effects on hen performance, egg quality, or plasma level of metabolites (proteins, glucose, uric acid, and cholesterol) and electrolytes (Na, K, Cl, P, and Ca). Overall plasma enzyme concentrations, particularly gamma-glutamyl transferase, were significantly (P < 0.01) lowest at the 10 and 20% levels of HS inclusion, or at the 4.5% HO level of inclusion of the hempseed products compared with the higher levels or control fed hens. Similar effects were also observed for plasma aspartate aminotransferase levels but with the HS enriched diets only (P < 0.05), particularly being lowest at the inclusion levels of 10 and 20% HS compared with the control. The results may imply a possible protective effect of HS- and HO-containing diets, particularly at 10% HS, 20% HS, and 4.5% HO levels, on liver damage/injury. In summary, both HO and HS appear to be well tolerated by laying hens as judged by markers of plasma clinical chemistry supporting the safety and efficacy of hemp products for use in laying hen rations.

Calcium and phosphorus dynamics in commercial laying hens housed in conventional or enriched cage systems.

Calcium (Ca) and phosphorus (P) dynamics in Shaver White hens (19-63 wk of age) were compared between enriched (EC) and conventional cage (CC) systems. Calcium and P intake and their levels in egg components and excreta were considered. Using commercial levels of production (4,836 hens), 10 test cages/system (24 hens/test cage) were used as replicate units. Enriched cages provided a nesting area, scratch pad, perches, and more floor space (643 cm(2)/hen ) than CC (468 cm(2)/hen). All birds were offered similar phase-fed diets based on wheat-soybean formulation and housed under semicontrolled environmental conditions for 11 periods (28 d each). Egg weight, production, and shell quality indices (egg specific gravity, shell weight, thickness, and percentage shell) were also measured. Data were analyzed as a repeated measures design using the MIXED procedure of SAS. Egg production, weight, and shell quality measurements were not significantly different between the 2 systems. On a DM basis, EC hens exhibited lower feed disappearance than CC hens (92.5 vs. 95.0 ± 0.61 g/hen per day, respectively; P < 0.01), and lower Ca and P excretions in manure compared with CC hens (Ca: 2.11 vs. 2.29 ± 0.04 g/hen per day, respectively; P: 0.619 vs. 0.643 ± 0.005 g/hen per day, respectively; P < 0.01). Even though eggs from EC compared with CC had lower Ca deposition (2.07 vs. 2.13 ± 0.01 g/hen per day, respectively; P < 0.0001) and output (38.3 vs. 38.8 ± 0.15 mg/g of egg, respectively; P < 0.05), both EC and CC systems exhibited similar Ca outputs in eggs when expressed as a proportion of Ca intake (56.5 vs. 56.6 ± 0.51% Ca intake, respectively). The overall mean P retention between EC and CC hens was not significantly different (-7.22 vs. -7.45 ± 0.71% P intake, respectively), but Ca retention was higher in EC than CC hens (-1.37 vs. -4.76 ± 0.89% Ca intake, respectively; P < 0.05). In addition to providing environmental enrichment, EC systems may help to reduce Ca and P excretions when compared with CC systems, thereby improving the utilization of these nutrients.

Production performance and nitrogen flow of Shaver White layers housed in enriched or conventional cage systems.

Despite the large number of studies examining the impact of cage systems on Ca and P nutrition, data are limited on the N balance of hens when housed under different systems. To this end, an experiment was conducted to assess N balance, manure characteristics, and indices of the performance of laying hens housed in 2 distinct caging systems. A total of 4,836 commercial Shaver White hens were housed in either enriched (EC) or conventional (CC) cages (average floor space per bird of 643 and 468 cm(2), respectively) under semicontrolled environmental conditions. Enriched cages provided hens with a curtained nesting area, scratch pad, and perches. Birds in both systems were phase fed similar layer diets for 11 periods (4 wk each). Data, expressed on a hen basis, were analyzed as repeated measures using the mixed model procedure of SAS. Lower feed disappearance (P < 0.01; 92.5 vs. 95.0 ± 0.6 g/d, DM basis) and manure output (P < 0.01; 79.8 vs. 91.3 ± 1.2 g/d, as-is basis, and 27.0 vs. 28.1 ± 0.2 g/d, DM basis) were observed in birds housed in EC compared with CC, respectively. Manure DM was 34.1 and 31.0 ± 0.3% for EC and CC, respectively. Egg production, feed conversion ratio, BW, egg weight, and egg mass were not significantly different between the 2 systems. Overall egg N output decreased with age for both cage systems and was not significantly different between the systems. Although no difference was observed in the overall manure N excretion (1.94 and 1.96 ± 0.02 g/d for EC and CC, respectively), hens housed in CC had a significantly (P < 0.05) higher N balance compared with those in the EC system (85.0 vs. 30.2 ± 13.6 mg/d, respectively), which could potentially be explained by a higher (P < 0.05) manure N excretion in the EC at the later stages of production. The current data provide estimates of the efficiency of N utilization in laying hens housed under different housing conditions.

Lipidomic characterization of omega-3 polyunsaturated fatty acids in phosphatidylcholine and phosphatidylethanolamine species of egg yolk lipid derived from hens fed flaxseed oil and marine algal biomass.

Membrane phospholipids, including phosphatidylcholine (PC) and phosphatidylethanolamine (PE), consist of distinct fatty acids occupying the sn-1 and sn-2 positions, reflecting the highly regulated nature of lipid biosynthesis. However, little is known about the influence of dietary lipids on the positional nature of fatty acids in tissues, including the enrichment of omega-3 polyunsaturated fatty acid (PUFA) in chicken egg yolk phospholipids. This study was undertaken to characterize the PC and PE species in egg lipids derived from Lohmann hens (n=10/treatment) randomly allocated to either a control (no supplementation), a flaxseed oil (FO) or a marine algal oil (MA) diet. Each of the FO or MA diets supplied three levels of total omega-3 PUFA (0.20, 0.40 and 0.60% of diet) that were provided for 6 weeks. A combination of multiplexed mass spectrometry (MS) experiments are used to determine total, isobaric, and position molecules for PC and PE in egg yolk. The distribution of phospholipids in the yolk was predominantly PC over PE (~72 vs. 23%, respectively) across treatments. The longer chain PUFA existed in the sn-2 position in the PC and PE. Although docosahexaenoic acid (22:6) formed isomers with fatty acids 16:0, 18:0 and 18:1; it was preferentially enriched in the egg in combination with 16:0 with both the FO and MA-fed groups in both lipid pools. All 22:6-containing isomers were enriched by ~2-fold more (P < 0.0001) with MA than FO, however, all isomers exhibited a plateau with the FO-fed group. In addition, the MS analyses of PCs revealed several isobaric species containing eicosapentaenoic acid (EPA, 20:5), however, in the PE, EPA formed only one isomer (i.e. in combination with 16:0). These results may assist to elucidate potential aspects regulating the limited enrichment of omega-3 PUFA, particularly EPA and docosahexaenoic acid (22:6) in chicken eggs.

Effect of dietary n-3 polyunsaturated fatty acids on the composition of cecal microbiome of Lohmann hens.

Supplementation of n-3 fatty acids to poultry diets is widely acknowledged for its role in enhancing poultry products, however, little is known about the compositional responses of gut microbial communities to type and dosage of these supplements.  Here, we compared the effects of n-3 polyunsaturated fatty acids (PUFA), supplied as alpha-linolenic acid (ALA) or docosahexaenoic acid (DHA), on the composition of bacterial communities in ceca of laying hens.  Corn-soybean basal diets were supplemented with either flaxseed oil (FO, ALA-rich) or marine algal biomass (MA, DHA-rich), and each supplied 0.20 and 0.60% of total n-3 PUFA in the diet.  Lohmann LSL-Classic laying hens (n = 10/treatment) were randomly allocated to one of the 4 diets.  After 8 weeks of feeding, blood, liver and cecal digesta samples were obtained for plasma glucose, fatty acids, and short chain fatty acids analyses, respectively.  The gut bacterial communities were characterized using genomic DNA extracted from cecal contents, whereby the V3-V4 hypervariable region of the 16S rRNA gene was sequenced using the Illumina Miseq® platform.  Firmicutes and Bacteroidetes were the predominant phyla in both the FO- and MA-fed groups.  The relative abundance of Tenericutes, often associated with immunomodulation, was relatively higher (P<0.0001) in the FO than MA group.  Although the relative abundance of Bacteroides was greater for the FO- than the MA-fed group, this genus was negatively correlated (P<0.05) with total n-3 PUFA in the liver at higher dosages of both FO- and MA-fed hens.  Higher dose of FO (0.60%) and both dosages of MA (0.20 and 0.60%) substantially enriched several members of Firmicutes (e.g., Faecalibacterium, Clostridium and Ruminococcus) which are known to produce butyrate.  Moreover, co-occurrence network analysis revealed that, in the FO 0.60- and MA 0.20-fed hens, Ruminococcaceae was the most influential taxon accounting for about 31% of the network complexity.  These findings demonstrate that supplementation of different type and level of n-3 PUFA in hens' diets could enrich microbial communities with potential role in lipid metabolism and health.

Interactive effects of calcium and top-dressed 25-hydroxy vitamin D3 on egg production, egg shell quality, and bones attributes in aged Lohmann LSL-lite layers1.

Calcium and vitamin D are critical in attenuating metabolic Ca insufficiency often observed in older hens. We investigated interactive effects of dietary Ca and top-dressed 25-hydroxy vitamin D3 (25OHD3) on egg production, eggshell quality (thickness, EST, and breaking strength, ESBS), serum Ca, kidney, liver, and bones attributes in 74-wk old Lohmann LSL-lite layers. A total of 4 levels of Ca (3.0, 3.5, 4.0, and 4.5%) and 3 levels of 25OHD3 (0, 69, and 138 µg/kg) were tested. All diets had basal level of 3,300 IU of vitamin D3/kg, were allocated to 84 individually housed hens (n = 7), and fed to 81 wk of age. Dual-energy X-ray absorptiometry was used to measure bone mineral content (BMC) and density (BMD) in left ulna, femur, and tibia at week 74 (13 spare birds, baseline) and week 81. Birds fed diets containing 3.0, 3.5, 4.0, and 4.5% Ca consumed 3.4, 4.1, 4.2, and 4.8 Ca g/b/d. Interaction (P < 0.05) between Ca and 25OHD3 was such that 25OHD3 linearly increased egg weight and percentage of eggs graded as jumbo (≥70 g) at 3.0, 3.5, and 4.5% Ca levels. There was no interaction (P > 0.05) between Ca and 25OHD3 on the rest of the parameters. Calcium increased EST and ESBS quadratically (P < 0.05), EST increased with Ca intake up to 4.1 g of Ca/b/d whereas Ca intakes (3.4 and 4.8 g of Ca/b/d) had poor ESBS relative to intermediate Ca intakes. Kidney ash concentration increased linearly (P = 009) with Ca intake. However, BMC and BMD in ulna, tibia, and femur declined (P < 0.05) at week 81 relative to baseline. In conclusion, top dressed 25OHD3 linearly increased egg weight at various Ca intakes with no negative effect on eggshell quality or bone attributes. Feeding 4.1 to 4.2 g of Ca/b/d improved eggshell quality, however, higher intake deteriorated eggshell quality and increased renal ash accumulation with no benefit on skeletal integrity.

Growth performance, apparent retention of components, and excreta dry matter content in Shaver White pullets (5 to 16 week of age) in response to dietary supplementation of graded levels of a single strain Bacillus subtilis probiotic.

Administered in adequate amounts, probiotics can be an alternative to antibiotic growth promoters in poultry production. This study evaluated dose response of a single strain of Bacillus subtilis (SSB, DSM29784) on growth performance, apparent retention (AR) of components, and excreta DM content in pullets. A basal corn-soybean meal diet was formulated to meet the specifications for grower (week 5 to 10) and developer (week 11 to 16) phases. In each phase, SSB was added to the basal diet to create 4 test diets: 0 (control, CON), 1.1E+08 (low; LSSB), 2.2E+08 (medium; MSSB), or 1.1E+09 (high; HSSB) CFU of SSB/kg of feed. All diets had TiO2 (0.5%) as a digestibility marker. A total of 720 day-old Shaver White chicks were placed in 48 cages (15 pullets per cage) and reared on a commercial antibiotic free diet for a 4-wk period. At the beginning of week 5, treatments were allocated based on cage BW (n = 12). Birds had free access to feed and water throughout. The BW, BW uniformity, feed intake (FI) and FCR were obtained weekly. Excreta was collected at the end of each phase for AR of DM, organic matter, CP, neutral detergent fiber, minerals, and AME, as well as excreta DM content. In response to SSB inclusion, BW improved in a linear (P < 0.005) and quadratic (P < 0.0001) manner in grower. FI decreased in a linear and quadratic pattern (P < 0.05, week 8 to 10) in grower, and linearly (P < 0.05) across the developer phase. Overall FCR improved in linear and quadratic pattern (P < 0.01) in the grower phase. In both the phases, AME improved in a quadratic pattern (P < 0.05). Inclusion of SSB had a linear reduction (P < 0.0001) in excreta moisture content in the grower phase. In summary, these results demonstrate that B. subtilis probiotic improved performance through enhanced nutrient utilization and reduced excreta moisture content indicating improved pullet gut health.

Effects of rearing and adult laying housing systems on medullary, pneumatic and radius bone attributes in 73-wk old Lohmann LSL lite hens1.

We determine interactive effect of a combined model of housing systems for rearing pullets and raising adult birds on bone quality. The LSL-Lite pullets were reared in either an aviary system (A) or conventional cages (C). At week 16, the birds were transferred either to the same housing type through the adult stage (A-A or C-C, for rearing and adult phase, respectively) or to enriched system (A-E or C-E, respectively). In the rearing stage, C cages housed 16 pullets/cage (week 0 to 6) and 8 pullets/cage (week 6 to 16) with a space allowance of 145 and 290 cm2/pullet, respectively; the rearing aviary housed 756 pullets/enclosure allowing 285 cm2/pullet (week 0 to 6) and 754 cm2/pullet (week 6 to 16). In the adult phase, C cages were 58.4 cm wide × 66.0 cm deep (482 cm2/pullet, 8 pullets/cage). For adult A, a commercial multi-tier aviary provided a space allowance >1000 cm2/hen system with litter area. Enriched system housed 60 birds/cage at 750 cm2/bird with perches, nest, and scratch mat. Bone samples were collected from the adult phase (week 73) and analysed for bone quality indices including bone breaking strength (BBS), total bone weight (TBW), ash content of the medullary (femur and tibia), pneumatic (humerus and keel), and radius bones. The A-A hens had the greatest (P < 0.05) TBW for medullary (femur and tibia) and pneumatic (humerus and keel) bones compared with hens from other housing models with exception of C-E hens. In addition, ash content was heavier (P = 0.048) for both A-A and C-E managed birds compared to the other housing. Birds in C-C system had the least score (P < 0.05) for TBW and ash content in all bone types. Regardless of the housing system, BBS was correlated (r = 0.60; P < 0.01) with the amount of ash and TBW, particularly for humerus and tibia. The results provide an insight on the impact of type of housing system in rearing and production stages on late cycle bone quality.

Effect of dietary supplementation of Bacillus subtilis DSM29784 on hen performance, egg quality indices, and apparent retention of dietary components in laying hens from 19 to 48 weeks of age.

The aim of the current study was to evaluate egg production, quality, and apparent retention (AR) of components in response to a multi-dose application of a single strain Bacillus subtilis (SSB; DSM29784) in a corn-soybean meal basal diet fed to hens (19 to 48 wk of age). The treatments consisted of a basal diet with either no probiotic (control, CON), 1.1E+08 (low, LSSB), 2.2E+08 (medium, MSSB) or 1.1E+09 (high, HSSB) CFU/kg of diet. A total of 336, 19-wk old Shaver White layers were used at a stocking density of 7 and 6 hens/replicate-cage in layer I (week-19 to 28) and layer II (week-29 to 48) phases, respectively. Evaluated variables included feed intake (FI), body weight (BW), feed conversion ratio (FCR), egg production, weight, mass, and egg quality (shell thickness, shell breaking strength, albumen height (AH), and Haugh unit (HU)). Excreta was collected at the end of week-28 for AR of components and apparent metabolizable energy (AME). Supplementation of SSB increased (P = 0.008) FI during peak egg-lay (week-24) and BW increased linearly (P = 0.019) in early layer II (week-32). In layer I, LSSB compared with CON increased EM (g/egg) by 3.3% (P = 0.049). In layer II, SSB inclusion tended to improve FCR (linear, P = 0.094; g FI: g EM). Although shell breaking strength was lowest at week-20 with HSSB (4.518 vs. 4.889 kgf for HSSB vs. CON; P = 0.045), AH and HU were improved at higher dose of SSB, in both phases (P ≤ 0.005). Apparent retention of dry matter, AME, and minerals were improved (P < 0.0001) in a dose response. Hence, while the low dose of B. subtilis DSM29784 improved hen performance and maintained egg quality in both phases, a higher dose of SSB improved the interior protein quality of eggs (AH and HU).

Egg production and quality responses of adding up to 7.5% defatted black soldier fly larvae meal in a corn-soybean meal diet fed to Shaver White Leghorns from wk 19 to 27 of age.

We examined egg production and quality responses of adding up to 7.5% defatted black soldier fly larvae meal (BSFLM) in a corn-soybean meal diet fed to pullets (19 to 27 wk of age). The concentration of CP and crude fat in BSFLM sample was 59.3 and 7.0% DM, respectively. A corn-soybean meal diet was formulated with 0 or 5.0 or 7.5% BSFLM and fed (n = 6) to a total of 108, 19-wk-old Shaver White pullets placed in conventional cages (6 birds/cage). The birds had free access to feed and water. Hen-day egg production (HDEP) and average egg weight were monitored daily and feed intake (FI) weekly. Egg quality parameters were assessed on individual eggs collected on the 5th d of wk 22, 24, and 26 and included individual EW (IEW), albumen height (HU), yolk color (YC), egg shell-breaking strength (SBS) and thickness (ST). A quadratic response (P < 0.02) was observed for HDEP, EW and egg mass. Specifically, birds fed 0 and 7.5% BSFLM diets had similar (P > 0.05) values for these parameters with birds fed 5.0% BSFLM showing lower (P < 0.05) HDEP than 0 or 7.5% BSFLM fed birds. The HDEP was 89.4, 84.8, and 87.8 for 0, 5.0, and 7.5% BSFLM, respectively. Feeding BSFLM linearly (P < 0.01) increased FI and feed conversion ratio (FCR) (FI/egg mass). There was no diet effect (P > 0.05) on IEW and HU, however, BSFLM linearly (P = 0.02) reduced CV of IEW. The IEW was 53.7, 52.3, and 53.0 g for 0, 5.0, and 7.5% BSFLM-fed birds, respectively and corresponding CV values of IEW were 7.9, 5.2, and 5.1%. Feeding BSFLM linearly (P < 0.01) increased YC, SBS, and ST. In conclusion, birds fed 7.5% BSFLM had similar HDEP and egg mass but poor FCR relative to corn-soybean meal diet without BSFLM. The effects of BSFLM on egg quality characteristics warrant further investigations.

Impact of dietary precursor ALA versus preformed DHA on fatty acid profiles of eggs, liver and adipose tissue and expression of genes associated with hepatic lipid metabolism in laying hens.

Dietary omega-3 polyunsaturated fatty acids (n-3 PUFA), including alpha-linolenic acid (ALA) and preformed longer chain PUFA (LCPUFA, particularly docosahexaenoic acid, DHA) differ in their egg LCPUFA enrichment efficiency. However, mechanisms leading to these differences are unclear. To this end, omega-3 PUFA contents in different lipid classes, including triacylglycerol (TAG) and total phospholipid (PL) in yolk, liver and adipose, as well as the expression of key hepatic enzymes in lipid metabolism were evaluated in laying hens in response to changes in dietary supply. Seventy Lohmann hens (n=10/treatment) consumed either a control diet (0.03% total omega-3 PUFA), or the control with supplementation (0.20%, 0.40% and 0.60% total omega-3 PUFA) from either flaxseed oil or algal product, as sources of ALA (precursor) or DHA (preformed), respectively. The study was arranged in a completely randomized design, and data were analyzed using the Proc Mixed procedure of SAS. ALA accumulated as a function of intake (P<0.0001) in total and lipid classes of yolk, liver and adipose (TAG only) for ALA- and DHA-fed hens. Unlike flaxseed oil, preformed-DHA contributed to greater (P<0.0001) accumulation of LCPUFA in yolk total PL and TAG pool, as well as adipose TAG. This may relate to elevated (P<0.0001) expression of acyl-CoA synthetase (ACSL1). No difference in hepatic EPA level in total lipids was noted between both treatment groups; EPAliver=2.1493x-0.0064; R2=0.70, P<0.0001 (x=dietary omega-3 PUFA). The latter result may highlight the role of hepatic EPA in the regulation of LCPUFA metabolism in laying hens.

Increasing Levels of Dietary Hempseed Products Leads to Differential Responses in the Fatty Acid Profiles of Egg Yolk, Liver and Plasma of Laying Hens.

The limited efficiency with which dietary alpha-linolenic acid (ALA) is converted by hens into docosahexaenoic acid (DHA) for egg deposition is not clearly understood. In this study, dietary ALA levels were increased via the inclusion of hempseed (HS) and hempseed oil (HO) in hen diets, with the goal of assessing the effects on the fatty acid (FA) profiles of total lipids and lipid classes in yolk, liver and plasma. Forty-eight hens were individually caged and fed one of six diets containing either HS:10, 20 or 30, HO:4.5 or 9.0 (%, diet) or a control (containing corn oil), providing a range (0.1-1.28 %, diet) of ALA. Fatty acid methyl esters of total lipids and lipid classes, including phosphatidyl choline (PtdCho) and ethanolamine (PtdEtn) in yolk, plasma and liver were then determined. Levels of n-3 FAs in both total lipids and lipid classes increased in all tissues. ALA and eicosapentaenoic acid (EPA) increased linearly, while docosapentaenoic acid and DHA increased quadratically. The FA profiles of yolk closely reflected levels in both plasma and liver. While ALA was highly concentrated in the triacylglycerol, it was low but equally distributed between PtdCho and PtdEtn in all tissues; however, the net accumulation was lower (P < 0.0001) in liver compared to yolk and plasma. Levels of EPA and ALA in yolk-PtdEtn were linearly (P < 0.0001; R (2) = 0.93) associated, and reflected those in liver-PtdEtn (P < 0.0001; R (2) = 0.90). In the liver, a strong inverse correlation (P < 0.0001; r = -0.94) between PL-DHA and ALA-to-EPA ratio in PtdEtn supports theories of low substrate availability, possibly limiting the conversion of ALA into DHA for egg enrichment.

Effect of flaxseed oil and microalgae DHA on the production performance, fatty acids and total lipids of egg yolk and plasma in laying hens.

The incorporation of omega-3 polyunsaturated fatty acids (PUFA) in the egg is dependent on both the transfer efficiency of preformed dietary omega-3 fatty acids to the eggs as well as endogenous PUFA metabolism and deposition. Employing an experimental design consisting of 70 Lohmann LSL-Classic hens (n=10/treatment) in a 6-week feeding trial, we examined the impact of graded levels of either flaxseed oil (alpha-linolenic acid, ALA) or algal DHA (preformed docosahexaenoic acid, DHA), each supplying 0.20%, 0.40% and 0.60% total omega-3s. The control diet was practically low in omega-3s. Study parameters included monitoring the changes of fatty acid contents in yolk, measures of hen performance, eggshell quality, total lipids and fatty acid contents of plasma. Data were analysed as a complete randomized design using Proc Mixed procedure of SAS. No significant differences were observed between treatments with respect to hen performance, eggshell quality and cholesterol content in plasma and egg yolk. Individual and total omega-3 PUFA in the yolk and plasma increased (P<0.0001) linearly as a function of total omega-3 PUFA intake. At the highest inclusion levels, DHA-fed hens incorporated 3-fold more DHA in eggs compared with ALA-fed hens (179±5.55 vs. 66.7±2.25mg/yolk, respectively). In both treatment groups, maximal enrichment of total n-3 PUFA was observed by week-2, declined by week-4 and leveled thereafter. In addition, accumulation of DHA in egg yolk showed linear (P<0.0001) and quadratic (P<0.05) effects for flaxseed oil (R2=0.89) and algal DHA (R2=0.95). The current data, based on defined level of total omega-3s in the background diet, provides evidence to suggest that exogenous as well as endogenous synthesis of DHA may be subject to a similar basis of regulation, and serve to highlight potential regulatory aspects explaining the limitations in the deposition of endogenously produced omega-3 LCPUFA.

Hempseed Products Fed to Hens Effectively Increased n-3 Polyunsaturated Fatty Acids in Total Lipids, Triacylglycerol and Phospholipid of Egg Yolk.

Hempseed products represent potential alternative feed ingredients for poultry. However, their usage is not currently approved due to a lack of data to support their safety and efficacy. In this regard, the current study was conducted to assess the impact of dietary concentration of hempseed (HS) products and duration of their feeding to hens on the polyunsaturated fatty acid (PUFA) composition of egg yolk lipids. In the current study, 48 Lohmann LSL-Classic hens were individually housed in metabolism cages, in a completely randomized design, and provided one of six diets (wheat-barley-soybean-based) containing either HS (10, 20 and 30 %), hempseed oil (HO; 4.5 and 9.0 %) or no hempseed product (control) over 12 weeks. Increasing alpha-linolenic acid (ALA) intake via increasing dietary hempseed product inclusion, significantly (p < 0.0001) increased the n-3 PUFA contents of yolk total lipid. The values of ALA increased by 12-fold (152 ± 3.56 and 156 ± 2.42 mg/yolk) and docosahexaenoic acid (DHA) by twofold to threefold (41.3 ± 1.57 and 43.6 ± 1.61 mg/yolk) over the control, for the highest levels of HS and HO inclusion, respectively. Increasing levels of hemp products in laying hen diets proved effective in manipulating the fatty acid profile of the total lipid, triacylglycerol (TAG) and total phospholipid (PL) fractions of yolks, enhancing the n-3 fatty acids and reducing the n-6/n-3 ratio. The latter benefit was achieved within 4 weeks of feeding hens either HS- or HO-containing diets.