Joint survival modelling for multiple species exposed to toxicants.

In environmental risk assessment (ERA), the multitude of compounds and taxa demands cross-species extrapolation to cover the variability in sensitivity to toxicants. However, only the impact of a single compound to a single species is addressed by the general unified threshold model of survival (GUTS). The reduced GUTS is the recommended model to analyse lethal toxic effects in regulatory aquatic ERA. GUTS considers toxicokinetics and toxicodynamics. Two toxicodynamic approaches are considered: Stochastic death (SD) assumes that survival decreases with an increasing internalized amount of the toxicant. Individual tolerance (IT) assumes that individuals vary in their tolerance to toxic exposure. Existing theory suggests that the product of the threshold zw and killing rate bw (both SD toxicodynamic parameters) are constant across species or compounds if receptors and target sites are shared. We extend that theory and show that the shape parameter ß of the loglogistic threshold distribution in IT is also constant. To verify the predicted relationships, we conducted three tests using toxicity studies for eight arthropods exposed to the insecticide flupyradifurone. We confirmed previous verifications of the relation- between SD parameters, and the newly established relation for the IT parameter ß. We enhanced GUTS to jointly model survival for multiple species with shared receptors and pathways by incorporating the relations among toxicodynamic parameters described above. The joint GUTS exploits the shared parameter relations and therefore constrains parameter uncertainty for each of the separate species. Particularly for IT, the joint GUTS more precisely predicted risk to the separate species than the standard single species GUTS under environmentally realistic exposure. We suggest that joint GUTS modelling can improve cross-species extrapolation in regulatory ERA by increasing the reliability of risk estimates and reducing animal testing. Furthermore, the shared toxicodynamic response provides potential to reduce complexity of ecosystem models.

Mechanistic Effect Modeling of Earthworms in the Context of Pesticide Risk Assessment: Synthesis of the FORESEE Workshop.

Earthworms are important ecosystem engineers, and assessment of the risk of plant protection products toward them is part of the European environmental risk assessment (ERA). In the current ERA scheme, exposure and effects are represented simplistically and are not well integrated, resulting in uncertainty when the results are applied to ecosystems. Modeling offers a powerful tool to integrate the effects observed in lower tier laboratory studies with the environmental conditions under which exposure is expected in the field. This paper provides a summary of the (In)Field Organism Risk modEling by coupling Soil Exposure and Effect (FORESEE) Workshop held 28-30 January 2020 in Düsseldorf, Germany. This workshop focused on toxicokinetic-toxicodynamic (TKTD) and population modeling of earthworms in the context of ERA. The goal was to bring together scientists from different stakeholder groups to discuss the current state of soil invertebrate modeling and to explore how earthworm modeling could be applied to risk assessments, in particular how the different model outputs can be used in the tiered ERA approach. In support of these goals, the workshop aimed at addressing the requirements and concerns of the different stakeholder groups to support further model development. The modeling approach included 4 submodules to cover the most relevant processes for earthworm risk assessment: environment, behavior (feeding, vertical movement), TKTD, and population. Four workgroups examined different aspects of the model with relevance for risk assessment, earthworm ecology, uptake routes, and cross-species extrapolation and model testing. Here, we present the perspectives of each workgroup and highlight how the collaborative effort of participants from multidisciplinary backgrounds helped to establish common ground. In addition, we provide a list of recommendations for how earthworm TKTD modeling could address some of the uncertainties in current risk assessments for plant protection products. Integr Environ Assess Manag 2021;17:352-363. © 2020 SETAC.