RESUMO
The leaf litters of tree species, Acacia pycnantha (Ap) and Eucalyptus camaldulensis (Ec), predominantly growing at an abandoned copper (Cu) mine and mine soils including controls, were assessed for determining the metal toxicity and bioavailability using earthworm species Eisenia fetida, in a microcosm. Significant reduction in body weight as well as mortality were observed when the worms were introduced into mine soil or its combination with mine Ap litter. Virtually, there were no juveniles when the worms were fed on substratum that contained mine soil or mine leaf litter. The extent of bioaccumulation was dependent on water-soluble fraction of a metal in soil. The accumulation of cadmium, lead and copper in worm tissue was significantly more in treatments that received mine soil with or without mine leaf litter. However, the tissue concentration of zinc did not differ much in earthworms irrespective of its exposure to control or contaminated samples. Mine leaf litter from Ec, a known Cu hyperaccumulator, was more hospitable to earthworm survival and juvenile than that of Ap litter. Validation of the data on bioaccumulation of metals indicated that the mine leaf litter significantly contributed to metal bioavailability. However, it was primarily the metal concentration in mine soil that was responsible for earthworm toxicity and bioavailability. Our data also indicate that detrivores like earthworm is greatly responsible for heavy metal transfer from mines into the ecosystem.
Assuntos
Oligoquetos/efeitos dos fármacos , Poluentes do Solo/toxicidade , Acacia/química , Animais , Disponibilidade Biológica , Cádmio/farmacocinética , Cádmio/toxicidade , Cobre/farmacocinética , Cobre/toxicidade , Monitoramento Ambiental , Eucalyptus/química , Chumbo/farmacocinética , Chumbo/toxicidade , Oligoquetos/metabolismo , Folhas de Planta/química , Reprodutibilidade dos Testes , Solo/química , Poluentes do Solo/farmacocinética , Árvores/química , Zinco/farmacocinética , Zinco/toxicidadeRESUMO
Plants play a key role in the accumulation of metals in contaminated environment. Ephemeral plants, such as cyperus vaginatus, from the family Cyperaceae have been used in constructed wetlands to alter the biogeochemistry of waterlogged soils. High elemental content in wetlands often induces chemical changes in the root, stem and leaf of wetland plants. Elemental uptake and possible chemical changes in the roots of Cyperus vaginatus was investigated and compared with plants grown away from the wetland. Among the 9 heavy metals (Cr, Mn, Fe, Co, Ni, Cu, Zn, Cd, and Pb) and metalloid (As) measured, with the exception of Mn, all metals had higher content in the plant roots grown within the wetland. This was followed by plants grown near to the wetland that receive stormwater occasionally and then plants grown far from the wetland. The 3-D fluorescence spectra record showed notable differences in the chemical composition of roots grown in the three locations. The spectra combined with parallel factor analysis showed three dominant fluorescence components. Comparison of the fluorescence signatures showed a continuum of spectral properties constrained by the degree of metal contamination.
Assuntos
Arsênio/metabolismo , Cyperus/química , Cyperus/metabolismo , Metais Pesados/metabolismo , Poluentes do Solo/metabolismo , Poluentes Químicos da Água/metabolismo , Biodegradação Ambiental , Raízes de Plantas/química , Raízes de Plantas/metabolismo , Áreas AlagadasRESUMO
Systematic site survey for sample collection and analysis was conducted at a derelict copper (Cu) mine at Kapunda, South Australia. Cu concentrations in the soils at this former mine ranged from 65-10107 mg kg(-1). The pH and EC varied widely in the 3.9-8.4 and 152-7311 µS ranges, respectively. Nine plant species growing over the copper mine site were selected to screen for metal uptake to determine their suitability for phytoremediation. The Australian native tree species Eucalyptus camaldulensis indicated enrichment factor (EF) of 2.17, 1.89, and 1.30 for Cu, Zn, and Pb, respectively, suggesting that this species of tree can accumulate these metals to some degree. The stress-resistant exotic olive, Olea europaea exhibited EF of ≤ 0.01 for Cu, Cd, and Pb, and 0.29 for Zn, which is characteristic of an excluder plant. Acacia pycnantha, the Australian pioneer legume species with EF 0.03, 0.80, 0.32, and 0.01 for Cu, Zn, Cd, and Pb, respectively, emerged as another strong metal excluder and consequently as an ideal metal stabilizer.
Assuntos
Recuperação e Remediação Ambiental/métodos , Metais Pesados/metabolismo , Plantas/metabolismo , Austrália , Biodegradação Ambiental , Cobre/análise , Cobre/metabolismo , Recuperação e Remediação Ambiental/instrumentação , Metais Pesados/análise , Mineração , Plantas/química , Plantas/classificação , Austrália do Sul , Árvores/química , Árvores/classificação , Árvores/metabolismoRESUMO
Keeping the sources of pollution such as chromium (Cr) under a safe limit is a daunting challenge due to the negative impact of heavy metal bioaccumulation in vegetation and the concomitant human health exposure. We took a closer look at Sonchus asper by cultivating in the green house. It resulted in 80% germination when cultivated over nine different soils collected from the tannery dump site. The biochemical analytical techniques such as mass spectrometry indicated significant bioaccumulation of Cr in the plant tissue. As per the ICP-MS analysis, this annual herb resulted in the accumulation of 601 mg kg-1 of total Cr with 212 mg kg-1 in its shoot from soil samples containing up to 41 mg kg-1 of hexavalent Cr. The energy dispersive X-ray (EDX) spectroscopy of S. asper revealed a higher level of S element indicating a sulfate-Cr binding relation. Elevated content of Cr in soil (73,721 ± 65 mg kg-1) caused biochemical changes in the shoot of S. asper as indicated by the disappearance of Fourier transform infrared spectroscopy (FTIR) bands at 935 and 872 cm-1 and further revealing aliphatic -CH2 appearing as anti-symmetry νa(CH2) and symmetric vibration νs(CH2) at the band of 2920 and 2850 cm-1, respectively.
Assuntos
Cromo/metabolismo , Poluentes do Solo/metabolismo , Sonchus/fisiologia , Biodegradação Ambiental , Cromo/análise , Poluição Ambiental/análise , Germinação , Humanos , Metais Pesados/análise , Plantas/metabolismo , Solo/química , Microbiologia do Solo , Poluentes do Solo/análise , Instalações de Eliminação de ResíduosRESUMO
Chromium from tannery waste dump site causes significant environmental pollution affecting surrounding flora and fauna. The primary aims of this study were to survey vegetation, investigate the degree of soil pollution occurring near tannery waste dump site and make a systematic evaluation of soil contamination based on the chromium levels found in plants and earthworms from the impacted areas. This paper presents the pollution load of toxic heavy metals, and especially chromium, in 10 soil samples and 12 species of plants. Soil samples were analysed for heavy metals by using ICP-MS/ICP-OES method. Results indicated that Cr in soils exceeded soil quality guideline limits (SQGL). The total chromium present in the above ground parts of plants ranged from 1.7 mg kg-1 in Casuarina sp. to 1007 mg kg-1 in Sonchus asper. The Cr bioaccumulation in Eisenia fetida from tannery waste soil ranged from 5 to 194 mg kg-1. The high enrichment factor of Cr in S. asper and bioaccumulation factor in earthworms indicate that there is a steady increase of toxic chromium risk in this area, which could be correlated with the past dumping activity. Emphasis needs to be put on control measures of pollution and remediation techniques in such areas to achieve an ecologically sustainable industrialisation.
Assuntos
Cromo/metabolismo , Recuperação e Remediação Ambiental , Magnoliopsida/metabolismo , Oligoquetos/metabolismo , Poluentes do Solo/metabolismo , Animais , Biodegradação Ambiental , Resíduos Industriais/análise , Austrália do Sul , Curtume , Instalações de Eliminação de ResíduosRESUMO
Understanding plant behaviour in polluted soils is critical for the sustainable remediation of metal-polluted sites including abandoned mines. Post-operational and abandoned metal mines particularly in semi-arid and arid zones are one of the major sources of pollution by soil erosion or plant hyperaccumulation bringing ecological impacts. We have selected from the literature 157 species belonging to 50 families to present a global overview of 'plants under action' against heavy metal pollution. Generally, all species of plants that are drought, salt and metal tolerant are candidates of interest to deal with harsh environmental conditions, particularly at semi-arid and arid mine sites. Pioneer metallophytes namely Atriplex nummularia, Atriplex semibaccata, Salsola kali, Phragmites australis and Medicago sativa, representing the taxonomic orders Caryophyllales, Poales and Fabales are evaluated in terms of phytoremediation in this review. Phytoremediation processes, microbial and algal bioremediation, the use and implication of tissue culture and biotechnology are critically examined. Overall, an integration of available remediation plant-based technologies, referred to here as 'integrated remediation technology,' is proposed to be one of the possible ways ahead to effectively address problems of toxic heavy metal pollution. Graphical abstract Integrated remediation technology (IRT) in metal-contaminated semi-arid and arid conditions. The hexagonal red line represents an IRT concept based on remediation decisions by combination of plants and microbial processes.