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1.
Clin Nephrol ; 69(1): 18-23, 2008 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18218313

RESUMO

We retrospectively investigated 2,093 renal biopsy procedures performed between 1976 and 2000 at Tokai University Hospital. The study period was divided into 5-year intervals, and the frequencies of each renal disease, age and sex of patients were compared across the study period. Clinical diagnosis was based on WHO criteria. A total of 2,093 patients aged 8 months to 84 years underwent renal biopsy during the study period. The percentage of elderly patients who underwent renal biopsy increased from 1.2% in 1976 - 1980 to 9.9% in 1996 - 2000. IgAN was the most common disease in every 5-year period. CresGN showed an increase from 1 patient (0.3%) in 1976 - 1980 to 15 patients (4.0%) in 1996 - 2000. In contrast, the number of patients with PGN or BRH, MPGN significantly decreased during the study period. Although the criteria for renal biopsy and renal diseases detected are expected to change in the future, renal biopsy will remain an essential procedure for making a definite diagnosis, selection of optimum treatment, and prediction of prognosis.


Assuntos
Biópsia/métodos , Hospitais Universitários , Nefropatias/patologia , Rim/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Seguimentos , Humanos , Lactente , Japão , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos , Índice de Gravidade de Doença
2.
Oncogene ; 25(3): 470-9, 2006 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-16247481

RESUMO

Mammalian chromatin remodeling factor, SWI/SNF complex contains a single molecule of either Brm or BRG1 as the ATPase catalytic subunit. Here, we show that the SWI/SNF complex forms a larger complex with neuron-restrictive silencer factor (NRSF) and its corepressors, mSin3A and CoREST, in human nonsmall cell lung carcinoma cell lines. We also demonstrate that the strong transcriptional suppression of such neuron-specific genes as synaptophysin and SCG10 by NRSF in these non-neural cells requires the functional SWI/SNF complex; these neuronal genes were elevated in cell lines deficient in both Brm and BRG1, whereas retrovirus vectors expressing siRNAs targeting integral components of SWI/SNF complex (Brm/BRG1 or Ini1) induced expression of these neuronal genes in SWI/SNF-competent cell lines. In cell lines deficient in both Brm and BRG1, exogenous Brm or BRG1 suppressed expression of these neuronal genes in an ATP-dependent manner and induced efficient and specific deacetylation of histone H4 around the NRSF binding site present in the synaptophysin gene by a large complex containing the recruited functional SWI/SNF complex. Patients with Brm/BRG1-deficient lung carcinoma have been reported to carry poor prognosis; derepression of NRSF-regulated genes including these neuron-specific genes could contribute to enhance tumorigenicity and also would provide selective markers for Brm/BRG1-deficient tumors.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/genética , Neoplasias Pulmonares/genética , Neurônios/metabolismo , Proteínas Repressoras/fisiologia , Fatores de Transcrição/fisiologia , Sequência de Bases , Western Blotting , Linhagem Celular Tumoral , Primers do DNA , Regulação da Expressão Gênica , Humanos , Imunoprecipitação , Reação em Cadeia da Polimerase Via Transcriptase Reversa
3.
Oncogene ; 22(6): 884-93, 2003 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-12584568

RESUMO

The oncogene function in primary epithelial cells is largely unclear. Recombination organ cultures in combination with the stable and transient gene transfer techniques by retrovirus and electroporation, respectively, enable us to transfer oncogenes specifically into primary epithelial cells of the developing avian glandular stomach (proventriculus). In this system, the epithelium and mesenchyme are mutually dependent on each other for their growth and differentiation. We report here that either stable or transient expression of v-src in the epithelium causes budding and migration of epithelial cells into mesenchyme. In response to the transient expression of v-Src or a constitutive active mutant of MEK, we observed immediate downregulation of the Sonic hedgehog gene and subsequent elimination of E-cadherine expression in migrating cells, suggesting the involvement of MAP kinase signaling pathway in these processes. v-src-expressing cells that were retained in the epithelium underwent apoptosis (anoikis) and detached from the culture. Continuous expression of v-src by, for example, Rous sarcoma virus (RSV) was required for the epithelial cells to acquire the ability to express type I collagen and fibronectin genes (mesenchymal markers), and finally to establish the epithelial-mesenchymal transition. These observations would partly explain why RSV does not apparently cause carcinoma formation, but induces sarcomas exclusively.


Assuntos
Epitélio/metabolismo , Mucosa Gástrica/metabolismo , Mesoderma/metabolismo , Proteína Oncogênica pp60(v-src)/metabolismo , Animais , Vírus do Sarcoma Aviário/metabolismo , Transformação Celular Neoplásica/metabolismo , Embrião de Galinha , Coturnix , Perfilação da Expressão Gênica , Técnicas de Transferência de Genes , Cinética , Proteínas Serina-Treonina Quinases/metabolismo , Transdução de Sinais/fisiologia , Estômago/citologia
4.
Mol Biochem Parasitol ; 28(3): 249-55, 1988 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-3386682

RESUMO

1H- and 13C-nuclear magnetic resonance (NMR) spectroscopy were used to study metabolites excreted by young adult Angiostrongylus cantonensis maintained aerobically in the presence of D-[13C6]glucose. End-products of glucose metabolism identified and quantitated by means of 1H-NMR were lactate, acetate and alanine, in the molar ratio of 1:0.13:0.05 for males and 1:0.07:0.04 for females. 13C-NMR analyses proved that all the three products originated from the glucose present in the medium.


Assuntos
Acetatos/análise , Alanina/análise , Angiostrongylus/metabolismo , Lactatos/análise , Metastrongyloidea/metabolismo , Animais , Feminino , Glucose/metabolismo , Espectroscopia de Ressonância Magnética , Masculino
5.
Am J Kidney Dis ; 37(2): 380-7, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11157381

RESUMO

We studied clinical and histologic parameters at the time of renal biopsy of 19 patients with idiopathic membranous nephropathy (IMN) to investigate the predictors for prognosis of IMN. Nineteen patients diagnosed by open renal biopsy between 1988 and 1993 and followed for at least 5 years were divided into two groups according to the latest follow-up renal function. Group I included 16 patients with normal renal function at the last follow-up point. Group II included three patients with end-stage renal failure at the last follow-up point. Antibodies to CD68, CD45RO, alpha-SMA, collagen IV, and collagen VI were used to investigate glomerular and interstitial changes in biopsy specimens by the indirect enzyme-labeled antibody method. Degree of global glomerulosclerosis, segmental glomerulosclerosis, adhesion to Bowman's capsule, and crescent formation were evaluated by light microscopy (periodic acid-Schiff, periodic acid-metheramine [PAM] staining). The difference between the two groups was analyzed by Mann-Whitney U: test. The number of interstitial cells, the number of interstitial CD45RO-positive cells, and increases of interstitial collagen IV and VI were found to be the most important factors for prognosis of IMN. These findings suggest that the extent of tubulointerstitial changes (cellular infiltration and fibrosis) determines the prognosis of renal function in IMN.


Assuntos
Glomerulonefrite Membranosa/patologia , Túbulos Renais/patologia , Adolescente , Adulto , Idoso , Biópsia , Creatinina/sangue , Feminino , Fibrose , Seguimentos , Glomerulonefrite Membranosa/fisiopatologia , Humanos , Imuno-Histoquímica , Rim/imunologia , Rim/patologia , Falência Renal Crônica/patologia , Túbulos Renais/citologia , Túbulos Renais/imunologia , Macrófagos , Masculino , Pessoa de Meia-Idade , Prognóstico , Estatísticas não Paramétricas , Linfócitos T
6.
Int J Parasitol ; 24(3): 417-9, 1994 May.
Artigo em Inglês | MEDLINE | ID: mdl-8070961

RESUMO

Proton nuclear magnetic resonance (1H NMR) spectroscopy was used to characterize the oxidized products in the serum lipoprotein lipids (LPL) of rabbits infected with Schistosoma japonicum. The loss of bis-allylic and allylic methylenes proton peaks (2.7 and 2.0 p.p.m.) of unsaturated systems of the LPL and the appearance of the signals of the conjugated diene systems of hydroperoxide (5.43-6.45 p.p.m.) and epidioxide (2.45 p.p.m.) of the fatty chains of the serum demonstrated the lipids degradation associated with the Schistosoma infection.


Assuntos
Lipídeos/sangue , Esquistossomose Japônica/sangue , Animais , Lipídeos/química , Espectroscopia de Ressonância Magnética , Oxirredução , Coelhos
7.
Neuroreport ; 8(6): 1435-7, 1997 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-9172149

RESUMO

We investigated the hypothesis that cell membrane function is abnormal in brains of subjects with Duchenne muscular dystrophy (DMD) using proton-nuclear magnetic resonance (NMR) spectroscopy of human brain extracts. The total amount of choline-containing compounds was significantly higher (about three times) than in normal controls and patients with other myopathies, while N-acetyl-L-aspartic acid and creatine were within the normal range. These findings indicate that abnormal cell membrane function may be correlated with the abnormal dystrophin or lack of dystrophin in the brain of patients with DMD.


Assuntos
Encéfalo/metabolismo , Colina/análise , Espectroscopia de Ressonância Magnética/métodos , Doenças Musculares/metabolismo , Distrofias Musculares/metabolismo , Adolescente , Adulto , Estudos de Casos e Controles , Criança , Pré-Escolar , Humanos , Doenças Musculares/congênito , Prótons
8.
Brain Dev ; 8(3): 280-4, 1986.
Artigo em Inglês | MEDLINE | ID: mdl-3766904

RESUMO

Two 10-year-old boys with mental retardation and myopathy which were present since birth are described. Both had elevated serum creatine phosphokinase (CK) and one of them had a positive family history. The clinical features were consistent with Fukuyama type congenital muscular dystrophy, but muscle biopsies suggested an inflammatory process. Adrenal cortical steroids were given and they were followed up until 10 years of age. Serum CK showed a significant response to the treatment, and mental retardation in case 1 and motor dysfunction in case 2 improved. It is postulated that an inflammatory process might be a causative factor in some patients with congenital muscular dystrophy.


Assuntos
Azatioprina/uso terapêutico , Encefalopatias/congênito , Distrofias Musculares/congênito , Prednisolona/uso terapêutico , Encefalopatias/tratamento farmacológico , Criança , Pré-Escolar , Creatina Quinase/sangue , Seguimentos , Humanos , Lactente , Recém-Nascido , Masculino , Músculos/patologia , Distrofias Musculares/tratamento farmacológico , Distrofias Musculares/patologia
9.
Brain Dev ; 19(2): 131-3, 1997 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9105660

RESUMO

The developmental changes in N-acetyl-L-aspartic acid (NAA) were assessed in human fetal and child brains by means of high resolution proton magnetic resonance spectroscopy (MRS). NAA was detected in the cerebral cortex and white matter of fetuses of 16 weeks' gestation. NAA increased gradually from 24 weeks' gestation and remarkably from 40 weeks' gestation to 1 year of age. The developmental changes in tissue NAA in postnatal brains were found to be similar to those of NAA/Cr on clinical proton MRS. As the neuronal cell density in the cerebral cortex decreases with dendritic maturation, an increase in NAA with age may reflect the normal and abnormal development of axons, dendrites and synapses as well as neuronal soma.


Assuntos
Ácido Aspártico/análogos & derivados , Química Encefálica/fisiologia , Encéfalo/crescimento & desenvolvimento , Neurônios/fisiologia , Adolescente , Adulto , Ácido Aspártico/metabolismo , Encéfalo/citologia , Encéfalo/embriologia , Senescência Celular/fisiologia , Córtex Cerebral/citologia , Córtex Cerebral/crescimento & desenvolvimento , Córtex Cerebral/metabolismo , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Espectroscopia de Ressonância Magnética , Gravidez
10.
Perit Dial Int ; 18(1): 52-9, 1998.
Artigo em Inglês | MEDLINE | ID: mdl-9527030

RESUMO

OBJECTIVE: In the present study, we investigated the influence of dialysate glucose on superoxide (O2-) generation by peripheral and peritoneal phagocytes in continuous ambulatory peritoneal dialysis (CAPD) patients. DESIGN: Peripheral polymorphonuclear leukocytes (PMNL) and mononuclear leukocytes (MNL), and peritoneal cells were isolated from peripheral blood and peritoneal effluents, respectively, and their oxidative metabolism was assessed by measuring O2- generation after stimulation with a soluble stimulant [phorbol myristate acetate (PMA), 1 mg/mL, Sigma Chemical, St. Louis, MO, U.S.A.] using the chemiluminescence method. Dialysate glucose effect on O2- generation was also studied in vitro by exposing peripheral PMNL and MNL from healthy controls to peritoneal dialysis fluid (PDF) containing glucose or amino acids at a neutral pH for different time periods. RESULTS: The amount of O2- generation by both peripheral and peritoneal phagocytes in CAPD patients was significantly higher than that in the control, and the response was greater in patients who were dialyzed with high glucose dialysate than those using low glucose dialysate. In an in vitro study, all incubated cells, except the control, showed suppression of O2- generation in the early dwell time (2 hr), and subsequently showed increased responses (peaking at 6 hr), although lower in degree than those observed in vivo. In contrast, amino acid-based PDF exhibited no such effect on O2- generation at identical pH with similar or lower osmolality. Furthermore, the respective increased or decreased oxidative responses with the increased or decreased PDF glucose concentrations in the same patient confirmed the positive effect of PDF glucose on phagocyte O2- generation. CONCLUSION: It is suggested that increased O2- generation by peritoneal and circulating phagocytes in CAPD patients is at least partly due to the enhancement of hexose monophosphate shunt activity by increasing glucose metabolism in phagocytes, and the increased O2- generation might be involved in long-term complications of CAPD. Therefore, a suitable alternative osmotic agent is needed to provide a more physiological environment to minimize the adverse effects of glucose on cell functions.


Assuntos
Soluções para Diálise , Solução Hipertônica de Glucose/farmacologia , Diálise Peritoneal Ambulatorial Contínua , Fagócitos/efeitos dos fármacos , Superóxidos/metabolismo , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/metabolismo , Masculino , Pessoa de Meia-Idade , Neutrófilos/efeitos dos fármacos , Neutrófilos/metabolismo , Concentração Osmolar , Cavidade Peritoneal/citologia , Fagócitos/metabolismo
11.
Intern Med ; 33(1): 60-3, 1994 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-8180447

RESUMO

A 40-year-old man developed slowly progressive muscle atrophy in his calf muscle. The patient became aware of asymmetry of his right big toe during in his teens; muscle atrophy was revealed at the age of 25 years. Multi-image examinations revealed "tethered cord syndrome" with spina bifida occulta. Spinal magnetic resonance image revealed a pathogenetic mechanism of asymmetry of muscle atrophy in the calf muscle. This suggested that spinal MRI imaging is one of the most useful diagnostic methods for this disease.


Assuntos
Perna (Membro)/patologia , Atrofia Muscular/patologia , Espinha Bífida Oculta/patologia , Adulto , Humanos , Imageamento por Ressonância Magnética , Masculino , Atrofia Muscular/complicações , Espinha Bífida Oculta/complicações
12.
J Emerg Med ; 20(3): 277-80, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11267817

RESUMO

Obturator hernia is a rare condition, and the prognosis of patients with this condition is poor. A retrospective study was performed on six patients with obturator hernia between 1993 and 1998. They had been diagnosed preoperatively by computed tomography (CT). The initial CT scan of the abdomen, including the pelvic area, revealed an incarcerated bowel in the obturator foramen of all six patients. All patients underwent laparotomy on the day of admission. Resection of the small bowel was performed in three patients, and release of the small bowel was performed in the remaining three patients. There were no perioperative deaths. In elderly women who have evidence by abdominal plain X-ray studies of small bowel obstruction, we recommend performing CT scan of the abdomen, including CT scan of the pelvic area, for detection of obturator hernia.


Assuntos
Hérnia do Obturador/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Adolescente , Idoso , Idoso de 80 Anos ou mais , Hérnia do Obturador/cirurgia , Humanos , Estudos Retrospectivos
13.
Rinsho Shinkeigaku ; 34(2): 157-62, 1994 Feb.
Artigo em Japonês | MEDLINE | ID: mdl-8194269

RESUMO

Two female cases, 23- and 21-year-old, of supravalvular aortic stenosis associated with cerebellar hypoplasia, retinitis pigmentosa and myopathy were reported. No family history of mental retardation and cardiovascular anomalies was found. There was no consanguinity between the parents. Pregnancy, labor and delivery were reported to be uncomplicated. When they visited to our hospital at the age of 13 and 11 years, they had short stature, characteristic facial appearance (eg, wide mouth, elongated philtrum, low nasal bridge and broad forehead) and supravalvular aortic stenosis. Neurological examination disclosed mental retardation, retinitis pigmentosa, muscle wasting and contracture of bilateral knee and ankle joints. Gait was unsteady and bradykinetic. Their smooth pursuiting ocular movements were saccadic. No nystagmus was recorded. Mild intention tremor was present. The muscles were slightly hypotonic, but deep tendon reflexes were hyperactive in the lower extremities. The sensory system was normal. Results of chromosome analysis and urine amino acid analysis were normal. The serum creatine kinase was elevated to 1,000-3,000 U. Muscle biopsy revealed nonspecific myopathic changes such as variability of fiber diameter in both fiber types. Neither cell infiltration nor deposits of fat or glycogen was found. Cranial MRIs performed at the age of 22 and 20 years disclosed cerebellar hypoplasia and moderate enlargement of the fourth ventricle. The two cases resembled clinically those of Williams syndrome, but the MRI findings were not consistent with those of the syndrome. The disorder is considered to be either Williams syndrome complicated by some other relatively rare clinical features, or another heredofamilial disease partly resembling Williams syndrome.


Assuntos
Estenose da Valva Aórtica/complicações , Ataxia Cerebelar/complicações , Face/anormalidades , Deficiência Intelectual/complicações , Doenças Musculares/complicações , Adulto , Estenose da Valva Aórtica/genética , Ataxia Cerebelar/genética , Saúde da Família , Feminino , Humanos , Deficiência Intelectual/genética , Doenças Musculares/genética , Síndrome
14.
Physiol Chem Phys Med NMR ; 28(1): 35-9, 1996.
Artigo em Inglês | MEDLINE | ID: mdl-8875805

RESUMO

Effects of mefloquine and quinine on human erythrocyte membranes were investigated by 1H-NMR spectroscopy. The observed spin-spin relaxation time (T2) of intracellular water protons was found to reflect water permeability of the membrane. The effects of these drug treatments on the erythrocytes were determined by the ratio of the T2 of control and experimental samples. Comparing the results of quinine and mefloquine treatments, quinine decreased the T2 ratio to 0.79 at 5 mM and mefloquine increased the ratio to 1.14 at 0.5 mM. These results demonstrated that quinine increases water permeability through the membranes whereas mefloquine decreases it.


Assuntos
Antimaláricos/farmacologia , Água Corporal/efeitos dos fármacos , Eritrócitos/química , Eritrócitos/efeitos dos fármacos , Mefloquina/farmacologia , Quinina/farmacologia , Adulto , Permeabilidade da Membrana Celular/efeitos dos fármacos , Membrana Eritrocítica/efeitos dos fármacos , Humanos , Técnicas In Vitro , Espectroscopia de Ressonância Magnética , Masculino , Prótons
15.
Physiol Chem Phys Med NMR ; 31(2): 103-8, 1999.
Artigo em Inglês | MEDLINE | ID: mdl-10816762

RESUMO

In the development of the human cerebellum, the intracellular metabolites were monitored during the period from the fetus to childhood by in vitro high resolution proton (1H) magnetic resonance (MR) spectroscopy. The spectra from fetus (15-30 post-menstrual weeks; n = 3), infant (1-24 months of age; n = 6) and child (7-14 years of age; n = 5) groups showed resonances from seventeen different metabolites. The level of N-acetylaspartate (NAA), one of the metabolites, was observed in age-dependent increases, two- and three-fold increases for infant and child groups from the NAA of the fetus group, respectively. The rapid increases in the creatine (Cre) level (approximately three-fold) in the fetus and infant groups were observed in the child group (approximately four-fold). Taurine (Tau) was noted at the highest concentration in the fetus group. Slight increases in concentrations of alanine, glutamate, glutamine, and glycine and a significant increase in the concentration of N-acetylaspartylglutamate were also noted in the fetus and infant groups. Other metabolite concentrations did not change significantly throughout the studied age groups. These findings indicate that synthesis of metabolites, especially of NAA and Cre, during the development of the cerebellum are closely correlated with mitochondrial energy metabolism, and as such, may reflect mitochondrial integrity in the cerebellum.


Assuntos
Cerebelo/embriologia , Cerebelo/crescimento & desenvolvimento , Desenvolvimento Embrionário e Fetal , Envelhecimento , Aminoácidos/metabolismo , Ácido Aspártico/análogos & derivados , Ácido Aspártico/metabolismo , Cerebelo/metabolismo , Criança , Pré-Escolar , Creatinina/metabolismo , Dipeptídeos/metabolismo , Feto , Humanos , Lactente , Espectroscopia de Ressonância Magnética/métodos
16.
Physiol Chem Phys Med NMR ; 29(1): 55-61, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-9353958

RESUMO

1H-NMR spectroscopy with 'Hahn' spin-echo pulse sequence has been employed to investigate the metabolic profiles of sera of chronic patients with schistosomiasis japonica, and compared with those of healthy volunteers and former patients who had been treated successfully. 1H-NMR clearly detected 2,3-butanediol and leucine, and markedly elevated levels of choline in sera from the chronic patients. Profiles of the sera from former patients were essentially similar to those from healthy volunteers, except that ketone bodies (3-hydroxybutyrate and acetone) were detectable in sera from 58% of the former patients but not in those of the normal controls patients.


Assuntos
Butileno Glicóis/sangue , Colina/sangue , Leucina/sangue , Esquistossomose Japônica/sangue , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Doença Crônica , Feminino , Humanos , Espectroscopia de Ressonância Magnética , Masculino , Pessoa de Meia-Idade
17.
Physiol Chem Phys Med NMR ; 32(1): 13-9, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10970043

RESUMO

In natural aging of spirits or wine, the dynamic structure of ethanol-water clusters changes to a smaller and more uniform state. Through experience we know that naturally aged ones have higher metabolism than the non-aged ones. Also, the same effect as natural aging can be obtained in various types of spirits or wines by the treatment for a period of time with soft ultrasonic wave (US). In this study, we compared ethanol metabolism in human subjects dosed with non-treated white wine (control = CON) and with US treated wine. Ethanol levels in human sera were followed by 400 MHz 1H-NMR spectroscopy after administration of wine doses. Experimental results indicated that ethanol metabolism was enhanced 18% in subjects when US treated wine was used rather than when non-treated (CON) was used. Other experiments using rabbits showed that a 20% ethanol-aqueous solution was absorbed 18% more rapidly by the group dosed with US wine than by the CON group. From these experimental facts, it was theorized that ethanol metabolism depends on the rapidity of ethanol absorption in the human body. And it can be concluded that US treatment brings about the same effect on spirits or wines as natural aging.


Assuntos
Etanol/sangue , Etanol/efeitos da radiação , Irradiação de Alimentos/métodos , Espectroscopia de Ressonância Magnética/métodos , Ultrassom , Vinho/análise , Vinho/efeitos da radiação , Animais , Análise Química do Sangue/métodos , Relação Dose-Resposta à Radiação , Humanos , Masculino , Taxa de Depuração Metabólica/efeitos da radiação , Prótons , Coelhos
18.
Physiol Chem Phys Med NMR ; 21(2): 165-70, 1989.
Artigo em Inglês | MEDLINE | ID: mdl-2608732

RESUMO

13C-nuclear magnetic resonance (NMR) spectroscopy was used to identify metabolites excreted by Angiostrongylus cantonensis eggs which had been maintained aerobically in the presence of D-[13C6] glucose. Using 13C-NMR we proved that lactate, acetate and alanine originated from glucose present in the medium via glycolysis. Aminooxyacetate, an inhibitor of alanine transferase, inhibited simultaneously alanine production and the ability to take up glutamate, aspartate and valine from the medium. In addition, we demonstrated that these amino acids can serve as amino group donors of the pyruvate to alanine transamination system in the eggs.


Assuntos
Glucose/metabolismo , Óvulo/metabolismo , Aminoácidos/metabolismo , Ácido Amino-Oxiacético/farmacologia , Angiostrongylus , Animais , Transporte Biológico/efeitos dos fármacos , Isótopos de Carbono , Feminino , Glicólise , Marcação por Isótopo/métodos , Espectroscopia de Ressonância Magnética/métodos , Modelos Biológicos
19.
Nihon Koshu Eisei Zasshi ; 41(5): 441-51, 1994 May.
Artigo em Japonês | MEDLINE | ID: mdl-8049512

RESUMO

Students, 60 females (mean age of 19 years) and 54 male (mean age of 23.8 years), were studied for relationships between serum vitamin A concentration (V-A level) and subjective health (by CMI method), birth order, blood and urinary parameters, dietary intake and food preferences. V-A level for females was significantly lower than for males. V-A level significantly and positively correlated to the parameters of hepatic enzymes (gamma-GTP, GOT, GPT), lipid (T-chol, TG) and urinary Na/K. The V-A level showed independent significant correlation to gamma-GTP in multiple regression analysis. V-A level of those born second in birth order showed a tendency to be lower than those first and third in birth order. In females, subjects without subjective CMI complaints had higher level of V-A than those with complaints. This tendency was not seen in males except for items related to the respiratory system. V-A level showed an apparent increase corresponding to daily intake of soybean and vegetables in females, and with amounts of drinking or smoking in male. In males, V-A levels showed a significant positive correlation to serum oxygen peroxide measured by malondialdehyde and did not show an anti-peroxidation effect. In addition positive correlation to total cholesterol was observed in males.


Assuntos
Análise Química do Sangue , Dieta , Preferências Alimentares , Nível de Saúde , Urinálise , Vitamina A/sangue , Adulto , Feminino , Humanos , Masculino , Análise de Regressão
20.
Nihon Jinzo Gakkai Shi ; 38(12): 595-602, 1996 Dec.
Artigo em Japonês | MEDLINE | ID: mdl-9014479

RESUMO

To clarify the role of genes related to angiotensin-I converting enzyme (ACE), the author investigated polymorphism of the ACE gene in 60 patients undergoing chronic ambulatory peritoneal dialysis (CAPD) and 50 patients undergoing hemodialysis (HD). One hundred healthy subjects were used as controls. The polymorphism was classified into three genotypes, II, ID and DD, according to insertion (I) and deletion (D) using the polymerase chain reaction method. In dialysis patients (CAPD or HD, n = 110), 21.8% had the II genotype, 48.2% the ID genotype, and 30.0% the DD genotype. There was a significant difference in allele frequency between normal subjects (n = 100) (J = 0.63, D = 0.37) and dialysis patients (I = 0.46, D = 0.54) (chi 2 = 12.321, p < 0.001). The mean plasma ACE activity was 9.9 +/- 1.6 IU/l in CAPD patients with the II genotype, 11.6 +/- 4.7 IU/l in CAPD patients with the ID genotype, and 14.5 +/- 3.5 IU/l in CAPD patients with the DD genotype. The mean rate of decrease in residual urinary volume was 0.8 +/- 0.7% per month in CAPD patients with the II genotype 1.4 +/- 1.3% per month in CAPD patients with the ID genotype, and 2.5 +/- 2.0% per month in CAPD patients with the DD genotype. These data showed a significant decrease in urinary volume in CAPD patients with the DD genotype (p < 0.05). The mean rate of decrease in residual urinary volume was positively correlated with the plasma ACE activity (r = 0.13389, p < 0.02). In CAPD patients, the mean cardiothoracic ratio was 46.6 +/- 3.5% in cases with the II genotype, 47.6 +/- 5.5% in cases with the ID genotype, and 52.9 +/- 8.4% in cases with the DD genotype. These data indicated significant cardiac enlargement in DD genotype cases. It can be concluded that CAPD patients with the DD genotype lost their residual renal function more rapidly and had a larger heart, than patients with the other genotypes.


Assuntos
Peptidil Dipeptidase A/genética , Diálise Peritoneal Ambulatorial Contínua , Polimorfismo Genético , Adulto , Idoso , Feminino , Frequência do Gene , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Diálise Renal
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