1.
Methods Mol Biol
; 848: 385-94, 2012.
Artigo
em Inglês
| MEDLINE
| ID: mdl-22315082
RESUMO
The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii, is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction.