RESUMO
BACKGROUND: Ducks are the natural reservoir of influenza A virus and the central host for highly pathogenic avian influenza (H5N1), while domestic ducks rearing in semi-scavenging system could serve as re-assortment vessels for re-emerging new subtypes of influenza viruses between birds to human. Avian influenza virus (AIV) surveillance in Bangladesh has been passive, relying on poultry farmers to report suspected outbreaks of highly pathogenic H5N1 influenza. Here, the results of an active surveillance effort focusing on the semi-scavenging ducks are presented. RESULT: A total of 2100 cloacal swabs and 2100 sera were collected from semi-scavenging ducks from three wintering-sites of Bangladesh during three successive winter seasons, December through February in the years between 2009 and 2012. Virus isolation and identification were carried out from the cloacal swabs by virus propagation in embryonated hen eggs followed by amplification of viral RNA using Avian influenza virus (AIV) specific RT-PCR. The overall prevalence of avian influenza type A was 22.05% for swab samples and 39.76% ducks were sero-positive for avian influenza type A antibody. Extremely low sero-prevalence (0.09%) of AIV H5N1 was detected. CONCLUSIONS: Based on our surveillance results, we conclude that semi-scavenging ducks in Bangladesh might play important role in transmitting Avian Influenza virus (AIV) type A. However, the current risk of infection for humans from domestic ducks in Bangladesh is negligible. We believe that this relatively large dataset over three winters in Bangladesh might create a strong foundation for future studies of AIV prevalence, evolution, and ecology in wintering sites around the globe.
Assuntos
Patos , Virus da Influenza A Subtipo H5N1 , Vírus da Influenza A/isolamento & purificação , Estudos Soroepidemiológicos , Animais , Bangladesh , Vírus da Influenza A/classificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterináriaRESUMO
The aim of this cross-sectional study was to assess the knowledge, attitudes and prevention practices (KAP) among the garment factory worker population in Bangladesh regarding a historical dengue outbreak in 2019. A total of 400 participants were selected by simple random sampling, and questionnaire-based interviews were conducted. The average score of knowledge, attitude and prevention practice was 8.33 ± 2.35, 6.32 ± 1.20 and 6.31 ± 1.50, respectively. Only 76 out of 400 participants (19%) scored above 10 (all university-educated). Participant workers reported both negative and positive attitudes regarding dengue fever (DF). Negative attitudes included an expectation of increased mortality and strained family finances from DF attacks. A significantly high number of participants (92%) believed that death from DF was inevitable. Positive attitudes included optimism about DF eradication potentials and eagerness to help and donate blood to sick relatives. Participants primarily learned about the DF prevention from mass media (244/400; 61.0%) and social media (97/400; 24.25%). The most popular prevention measures adopted were mosquito repellent incense (344/400; 86.0%) and mosquito nets (389/400; 97.25%). While most participants (358/400; 89.5%) cleaned areas where mosquitos lay eggs, only 169 out of 400 (42.25%) regularly treated with chemical sprays. Only 182 out of 400 (45.5%) reported receiving DF prevention training in the factory. Correlation between DF knowledge and education was statistically significant (r = .38, p < .01, n = 398). Correlation between DF knowledge and work experience was insignificant (r = .01, p > .01, n = 398). Age and DF knowledge were not correlated (r = 0.07, p > .01, n = 398). In conclusion, gaps in KAP for dengue could be addressed by government-sponsored educational programmes that utilize the power of mass/social media for dengue prevention and control. More KAP surveillance studies are needed for other sectors of the society.
Assuntos
Dengue , Conhecimentos, Atitudes e Prática em Saúde , Animais , Bangladesh/epidemiologia , Vestuário , Estudos Transversais , Dengue/epidemiologia , Dengue/prevenção & controle , Dengue/veterinária , Surtos de Doenças/prevenção & controle , Humanos , Óvulo , Inquéritos e QuestionáriosRESUMO
AIM: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. MATERIALS & METHODS: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. RESULTS: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. CONCLUSION: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.