A multicentre, open-label, single-arm phase II trial of the efficacy and safety of sclerotherapy using 3% polidocanol foam to treat second-degree haemorrhoids (SCLEROFOAM).

BACKGROUND: The aim of the present study was to evaluate the efficacy and safety of 3% polidocanol foam for treating 2nd-degree haemorrhoids. METHODS: A multicentre, open-label, single-arm, phase 2 trial involving 10 tertiary referral centres for haemorrhodal disease (HD) was performed. Between January and June 2019, patients with 2nd-degree haemorrhoids were prospectively included in this study. The primary outcome was to establish the success rate after one sclerotherapy session in terms of complete resolution of bleeding episodes one week after the injection. The Hemorrhoidal Disease Symptom Score (HDSS), the Short Health Scale for HD (SHS-HD) score and the Vaizey incontinence score were used to assess symptoms and their impact on quality of life and continence. Pain after the procedure, subjective symptoms and the amount and type of painkillers used were recorded. Patients were followed up for 1 year. RESULTS: There were 183 patients [111 males; 60.7%, mean age 51.3 ± 13.5 (18-75) years]. Complete resolution of bleeding was reached in 125/183 patients (68.3%) at 1 week and the recurrence rate was 12% (15/125). Thirteen patients (7.4%) underwent a second sclerotherapy session, while only 1 patient (1.8%) had to undergo a third session. The overall 1-year success rate was 95.6% (175/183). The HDSS and the SHS score significantly improved from a median preoperative value of 11 and 18 to 0 and 0, respectively (p < 0.001). There were 3 episodes of external thrombosis. No serious adverse events occurred. CONCLUSIONS: Sclerotherapy with 3% polidocanol foam is a safe, effective, painless, repeatable and low-cost procedure in patients with bleeding haemorrhoids.

Use of the Martius advancement flap for low rectovaginal fistulas.

AIM: The percentage recurrence after any surgical treatment for low rectovaginal fistula (LRVF) is unacceptably high. The aim of this study was to evaluate the short- and long-term results of the Martius procedure in a carefully selected series of patients with a LRVF of at least 1 cm diameter who had had at least two previous surgeries or in the presence of chronically inflamed local tissues. METHOD: Between January 2009 and April 2017, 24 patients with the abovementioned features were prospectively included in this study. Success was defined both as the absence of any subjective symptoms and the fistula, as confirmed by evaluation under anaesthesia. Postoperative complications were assessed using the Clavien-Dindo classification. Quality of life (SF-12 score), quality of sexual life [Female Sexual Function Index (FSFI) score] and continence [Cleveland Clinic Incontinence Score (CCIS)] were also determined pre- and postoperatively. RESULTS: The mean follow-up was 42 ± 29 months (range 3-101 months). The overall success rate was 91.3% (22/24 patients). The median operation time was 50 min (range 45-70 min), and the median hospital stay was 3.5 days (range 3-5 days). No major complications occurred. Pre- and postoperative CCIS did not differ [1 (range 0-3.5)]. The postoperative SF-12 score improved both in terms of the physical (33.6 ± 7.2 vs 50.8 ± 7.8; P < 0.001) and mental (32.6 ± 6.7 vs 56.3 ± 7.8; P < 0.001) components. FSFI improved from 19.5 ± 6.6 to 24.4 ± 6.3 (P < 0.001). CONCLUSION: The Martius procedure should be considered as the first-line method of treatment in carefully selected cases of LRVF.

Microbial dynamics during shelf-life of industrial Ricotta cheese and identification of a Bacillus strain as a cause of a pink discolouration.

Dairy products are perishable and have to be preserved from spoilage during the food chain to achieve the desired shelf-life. Ricotta is a typical Italian soft dairy food produced by heat coagulation of whey proteins and is considered to be a light and healthy product. The shelf-life of Ricotta could be extended, as required by the international food trade market; however, heat resistant microflora causes spoilage and poses issues regarding the safety of the product. Next-generation sequencing (NGS) applied to the Ricotta samples defined the composition of the microbial community in-depth during the shelf-life. The analysis demonstrated the predominance of spore-forming bacteria throughout the shelf-life, mostly belonging to Bacillus, Paenibacillus and Clostridium genera. A strain involved in spoilage and causing a pink discolouration of Ricotta was isolated and characterised as Bacillus mycoides/weihenstephanensis. This is the first report of a food discolouration caused by a toxigenic strain belonging to the Bacillus cereus group that resulted the predominant strain in the community of the defective ricotta. These results suggest that the processing of raw materials to eliminate spores and residual microflora could be essential for improving the quality and the safety of the product and to extend the shelf-life of industrial Ricotta.

Cross-cultural adaptation of the CALCULATE instrument into Brazilian Portuguese: pressure injury in intensive care.

OBJECTIVE: To perform the cross-cultural adaptation of CALCULATE for Brazilian Portuguese. METHOD: A methodological study conducted from January to December 2021, divided into six stages: translation, synthesis, back-translation, expert committee with the application of the content validity index, pre-testing in 40 adult patients, and submission to the authors. The study took place in the intensive care units of a public tertiary teaching hospital in the interior of the state of São Paulo, Brazil. The original CALCULATE has eight risk assessment items and is stratified with a score of 0-3 (high risk) and 4-8 (very high risk). RESULTS: After expert evaluation, the final content validity was 0.9. They suggested words and phrases that should undergo changes regarding textual equivalences, as well as definitions of acronyms and terminologies. In the pre-test, the items were assessed as suitable for understanding; only one item required additional explanation for adequacy. CONCLUSION: The cross-cultural adaptation of CALCULATE for Brazilian Portuguese was successfully performed, revealing a good content validity index, confirming the relevance and appropriateness of its items. CALCULATE is suitable for use in intensive care units and research and teaching centers.

Biocellulose-based hydrogel dressing as a strategy for the management of chronic arterial wounds.

PURPOSE: To evaluate using a biocellulose-based hydrogel as an adjuvant in the healing process of arterial ulcers. METHODS: A prospective single group quasi-experimental study was carried out with chronic lower limb arterial ulcer patients. These patients received biocellulose-based hydrogel dressings and outpatient guidance on dressing and periodic reassessments. The primary outcomes were the ulcer-healing rate and product safety, which were assessed by ulcer area measured in photographic records of pre-treatment and posttreatment after 7, 30, and 60 days. Secondary outcomes were related to clinical assessment by the quality-of-life scores (SF-36 and EQ-5D) and pain, evaluated by the visual analogue scale (VAS). RESULTS: Seventeen participants were included, and one of them was excluded. Six patients (37%) had complete wound healing, and all patients had a significant reduction in the ulcer area during follow-up (233.6mm2 versus 2.7mm2) and reduction on the score PUSH 3.0 (p < 0.0001). The analysis of the SF-36 and EQ-5D questionnaires showed a statistically significant improvement in almost all parameters analyzed and with a reduction of pain assessed by the VAS. CONCLUSIONS: The biocellulose-based hydrogel was safe and showed a good perspective to promoting the necessary conditions to facilitate partial or complete healing of chronic arterial ulcers within a 60-day follow-up. Quality of life and pain were positively affected by the treatment.

Nursing workload and severity of COVID-19 patients in the Intensive Care Unit.

OBJECTIVE: To evaluate the workload and severity of patients in the Intensive Care Unit (ICU) with COVID-19. METHOD: Cross-sectional, analytical study carried out in the ICU of a private hospital. All patients over the age of 18 with a diagnosis of COVID-19 admitted from September 2020 to June 2021 were included. Workload assessed by the Nursing Activities Score (NAS), and severity by the Sequential Organ Failure Assessment. Descriptive and inferential analyses were performed. RESULTS: 217 patients were included, mostly men, mean age 62.41 years, white, obese, non-smokers and sedentary. The average NAS was 84.79. Staffing was in line with legislation and NAS. NAS was not associated with severity. Severity was associated with higher age, gender, comorbidities, sedentary lifestyle, time on mechanical ventilation, hospitalization and death. CONCLUSION: Workload was high and not associated with severity or outcomes. Severity was associated with demographic and clinical conditions. This study shows the importance of staff sizing, with a view to promoting safety and quality of care.

Transperineal repair with bulbocavernosus muscle interposition for recto-urethral fistula.

AIM: The results of repair of recto-urethral fistulae (RUF) are presented using a bulbocavernosus muscle graft. METHOD: Prospectively collected data were reviewed on 11 patients with RUF operated on between 2003 and 2011. Of these, six were treated by a bulbocavernosus flap. Two RUF had occurred after prostatectomy, three after prostatectomy and radiotherapy and one after perineal trauma; all had a urinary diversion. RESULTS: Closure of the fistula was achieved in all patients and was maintained for the duration of the period of follow up (mean ± SD = 43.5 ± 24.7 months; range, 8-80 months) There were no complications. CONCLUSION: This new technique for the repair of RUF is safe and effective, especially in patients with complex postradiation RUF.

Internal Delorme's procedure for rectal outlet obstruction.

AIM: The outcome of the internal Delorme's procedure (IDP) for obstructed defaecation was assessed. METHOD: From October 2001 to March 2009, 167 patients with obstructed defaecation associated with rectal intussusception were operated on. Patients were selected on the basis of validated constipation and continence scores, clinical examination and defaecography. Seventy-six patients were treated by the IDP alone and 91 patients were treated by the IDP with a levatorplasty. Before surgery and after a mean ± SD follow up of 3.0 ± 1.5 years, patients were assessed using the Cleveland Clinic Incontinence and Constipation Score (CCIS and CCCS), the Obstructed Defecation Score (ODS), faecal urgency and the Patient Assessment of Constipation Quality of Life (PAC-QoL) questionnaire. RESULTS: Seventeen (10.2%) patients developed a postoperative complication including fissure-in-ano (4.2%), proctalgia (3.0%), suture-line dehiscence with stenosis (1.8%) and Clostridium difficile colitis (1.2%). Faecal urgency changed from 22% to 17.6% (P = 0.754). Tenesmus fell from 53.9% to 17.1% (P < 0.001). The CCCS and the ODS fell by 50% or more in 82.6% and 73.7% of the patients, respectively. The CCIS did not worsen significantly in patients who remained incontinent, and 45.7% of the previously incontinent patients regained normal continence. The CCCS decreased from 11 to 3 (P < 0.001) in the patients treated by the IDP and from 12 to 3 (P < 0.001) in the patients treated by the IDP with levatorplasty. The overall recurrence rate was 5.4%. The PAC-QoL showed a reduction of anxiety/depression and of physical and psychological discomfort (P < 0.001). CONCLUSION: The IDP is an effective and safe option for rectal outlet obstruction caused by rectal intussusception with excellent function and patient satisfaction.

Nursing protocols to reduce urinary tract infection caused by indwelling catheters: an integrative review.

OBJECTIVES: to analyze the production of knowledge in research articles about the effectiveness of nursing protocols for reducing indwelling urinary catheter dwell time and catheter-associated urinary tract infection rate in hospitalized adult and older patients. METHODS: an integrative review of three full articles, available in the MEDLINE Complete - EBSCO, Scopus and Web of Science databases, from 01/01/2015 to 04/26/2021. RESULTS: the three protocols reduced infection rates, and from the review/synthesis of their knowledge, a level IV body of evidence emerged to compose the nursing care process aimed at reducing indwelling urinary catheter dwell time and catheter-associated urinary tract infection. FINAL CONSIDERATIONS: this process gathers scientific evidence to support the elaboration of nursing protocols and, consequently, the conduction of clinical trials on its effectiveness in reducing urinary tract infection by indwelling urinary catheter.

Religious practice in the pandemic of COVID-19 and the nursing diagnoses.

PURPOSE: Identify the elements (defining characteristics, related factors, and risk factors) of the diagnoses of NANDA international impaired religiosity (00169), risk for impaired religiosity (00170), and readiness for enhanced religiosity (00171), in a period of social distancing in the pandemic of COVID-19, and associate them with the behavior of individual and collective religious practice, before and during the pandemic. METHODS: Survey study, released via social media to members of religious communities in Brazil. Data collection took place in June 2020, by online questionnaire. FINDINGS: Participants were 719 people, 563 (78.3%) were women, with a median age of 39 years (min 18-max 73), of Catholic religion (64.7%), with a median of 29 years of religious practice (min 0-max 70). The participants were from Southeast 652 (90.68%), South 49 (6.82%), Northeast 13 (1.82%), Midwest 4 (0.56%), and North 01 (0.14%) of Brazil. The increase of individual religious practice was associated with two diagnostic elements and the reduction of individual practice to nine elements. The reduction of collective religious practice was associated with seven diagnostic elements and the maintenance of the practice associated with five elements. The increase of collective religious practice was associated with five diagnostic elements. CONCLUSIONS: In individuals who presented during the pandemic reduction of individual religious practice, reduction of collective religious practice, and maintenance of collective religious practice, the elements of the diagnosis impaired religiosity were predominant. In individuals who presented increased practice of collective religious activity during the pandemic, the elements of the diagnosis readiness for enhanced religiosity were predominant. IMPLICATIONS FOR NURSING PRACTICE: This study highlights defining characteristics, risk factors, and related factors of the religiosity diagnoses presented due to social distancing in the pandemic; these should be screened during nursing consultations in primary health care.

Nursing workload associated with neonatal mortality risk: a cross-sectional study.

OBJECTIVES: to analyze the association between nursing workload and neonatal mortality risk in newborns admitted to the Neonatal Intensive Care Unit. METHODS: this is an observational, cross-sectional study conducted from January 2019 to January 2020. RESULTS: the sample consisted of 399 newborns, 55.4% male, Nursing Activities Score mean of 67.5%, and Score for Neonatal Acute Physiology Perinatal Extension mean of 17.7, revealed itself as a predictor of the risk of death, while gestational age, length of hospitalization, and the first-minute Apgar established a protective relationship. The correlation between workload and neonatal mortality was low (r= 0.23, p=0.0009). CONCLUSIONS: the workload of the nursing team is not associated with the risk of mortality in the Neonatal Intensive Care Unit, as measured by the Nursing Activities Score.

Calorimetry, morphometry, oxidative stress, and cardiac metabolic response to growth hormone treatment in obese and aged rats.

This study investigated the effects of growth hormone therapy on energy expenditure, lipid profile, oxidative stress and cardiac energy metabolism in aging and obesity conditions. Life expectancy is increasing in world population and with it, the incidence of public health problems such as obesity and cardiac alterations. Because growth hormone (GH) concentration is referred to be decreased in aging conditions, a question must be addressed: what is the effect of GH on aging related adverse changes? To investigate the effects of GH on cardiac energy metabolism and its association with calorimetric parameters, lipid profile and oxidative stress in aged and obese rats, initially 32 male Wistar rats were divided into 2 groups (n=16), C: given standard-chow and water; H: given hypercaloric-chow and receiving 30% sucrose in its drinking water. After 45 days, both C and H groups were divided into 2 subgroups (n=8), C+PL: standard-chow, water, and receiving saline subcutaneously; C+GH: standard-chow, water, and receiving 2 mg/kg/day rhGH subcutaneously; H+PL: hypercaloric-chow, 30% sucrose, receiving saline subcutaneously; H+GH: hypercaloric-chow, 30% sucrose, receiving rhGH subcutaneously. After 30 days, C+GH and H+PL rats had higher body mass index, Lee-index, body fat content, percent-adiposity, serum triacylglycerol, cardiac lipid-hydroperoxide, and triacylglycerol than C+PL. Energy-expenditure (RMR)/body weight, oxygen consumption and fat-oxidation were higher in H+GH than in H+PL. LDL-cholesterol was highest in H+GH rats, whereas cardiac pyruvate-dehydrogenase and phosphofrutokinase were higher in H+GH and H+PL rats than in C+PL. In conclusion, the present study brought new insights on aging and obesity, demonstrating for the first time that GH therapy was harmful in aged and obesity conditions, impairing calorimetric parameters and lipid profile. GH was disadvantageous in control old rats, having undesirable effects on triacylglycerol accumulation and cardiac oxidative stress.

Energy expenditure, lipid profile, oxidative stress, and cardiac energy metabolism after growth hormone treatment in obese young rats.

Obesity is rampant in modern society and growth hormone (GH) could be useful as adjunct therapy to reduce the obesity-induced cardiovascular damage. To investigate GH effects on obesity, initially 32 male Wistar rats were divided into two groups (n=16): control (C) was fed standard-chow and water and hypercaloric (H) was fed hypercaloric chow and 30% sucrose in its drinking water. After 45 days, both C and H groups were divided into two subgroups (n=8): C+PL was fed standard-chow, water and received saline subcutaneously; C+GH was fed standard-chow, water, and received 2 mg/kg/day GH subcutaneously; H+PL was fed hypercaloric diet, 30% sucrose in its drinking water, and received saline subcutaneously; and H+GH was fed hypercaloric diet, 30% sucrose in its drinking water, and received GH subcutaneously. After 75 days of total experimental period, H+PL rats were considered obese, having higher body weight, body mass index, Lee-index, and atherogenic index (AI) compared to C+PL. Obesity was accompanied by enhanced myocardial lipid hydroperoxide (LH) and lactate dehydrogenase (LDH), as well of depressed energy expenditure (RMR) and oxygen consumption(VO (2))/body weight. H+GH rats had higher fasting RMR, as well as lower AI and myocardial LH than H+PL. Comparing C+GH with C+PL, despite no effects on morphometric parameters, lipid profile, myocardial LH, and LDH activity, GH enhanced fed RMR and myocardial pyruvate dehydrogenase. In conclusion, the present study brought new insights into the GH effects on obesity related cardiovascular damage demonstrating, for the first time, that GH regulated cardiac metabolic pathways, enhanced energy expenditure and improved the lipid profile in obesity condition. Growth hormone in standard fed condition also offered promising therapeutic value enhancing pyruvate-dehydrogenase activity and glucose oxidation in cardiac tissue, thus optimizing myocardial energy metabolism.

Effect of breast myopathies on quality and microbial shelf life of broiler meat.

To evaluate the impact of emerging myopathies on meat quality and microbial shelf life, 48 normal, 48 white striped (WS), and 48 wooden breasts (WB) were stored for 11 d at 4°C aerobically and analyzed at 24, 72, 120, 168, 216, and 264 h post-mortem. Normal breasts showed lower (P < 0.001) redness index (-0.88 vs. -0.41 and -0.43) and cooking losses (22.0 vs. 23.8 vs. 26.9%) than those of WS and WB meat. Normal and WS breasts exhibited higher protein content than that in WB meat (23.9 and 23.2 vs. 21.4%; P < 0.001). Normal meat also had a lower ether extract content than that in WB meat (1.09 vs. 1.88%; P < 0.001), with intermediate values for WS meat. Normal breasts exhibited higher saturated fatty acid (FA) rate (31.3 vs. 28.0% of total FA on average) and lower unsaturated FA rate (68.7 vs. 72.0%) than those in WS and WB meat (P < 0.001). Differences were mainly due to polyunsaturated FA (30.5% in normal vs. 35.3 and 35.4% in WS and WB meat; P < 0.001). Normal breasts had higher initial total viable count (TVC) and a shorter TVC lag phase than those of WS and WB meat (46.3 vs. 85.2 and 77.8 h). The microbial shelf life threshold (7 log10 CFU TVC/g) was achieved first in normal (130 h) and then in WS (149 h) and WB (192 h) meat. TVC and Pseudomonas spp. counts were significantly higher in normal than those in the affected breasts between 72 and 216 h of storage. Enterobacteriaceae spp. and lactic acid bacteria counts were significantly higher in normal meat, lower in WB meat, and intermediate in WS meat until 216 h. All differences in microbial targets across meat types disappeared by 264 h of storage. Further studies are necessary to elucidate the factors and the mechanisms that may modulate microbial growth and composition during storage in broiler breast meat affected by myopathies.

Impacto psicológico na equipe do SAMU 192 durante pandemia COVID-19 / Pandemic's Effect on SAMU 192 Workers' Mental Health / Impacto de la pandemia en la salud mental de los trabajadores de SAMU 192

Resumo Introdução: O Serviço de Atendimento Móvel de Urgência - SAMU 192 é o componente pré-hospitalar móvel instituído pelo Ministério da Saúde no Brasil. Com a pandemia COVID-19, o serviço passou a atender os pacientes acometidos pela doença. Esse cenário trouxe grande tensão para os profissionais, o que tornou relevante compreender as alterações psicológicas vivenciadas pela equipe, a fim de proporcionar uma assistência psicológica adequada e o bem-estar aos mesmos. Objetivo: Analisar os níveis de depressão, ansiedade e estresse na equipe do SAMU 192. Metodologia: Estudo transversal, exploratório-descritivo, com abordagem quantitativa. Aplicou-se a versão reduzida da escala Depression, Anxiety and Stress Scale‐21 (DASS-21) na equipe do SAMU 192, uma escala tipo Likert de quatro pontos que avalia os níveis de depressão, ansiedade e estresse. Resultados: Participaram da pesquisa 54 profissionais do SAMU 192, 78,8% da equipe total. A média de idade foi de 37,41 anos, sendo a maioria do sexo feminino. A maioria dos profissionais apresentaram níveis normais de depressão, ansiedade e estresse, entretanto, os casos de alterações representaram 27,77%, 24,08% e 18,51% respectivamente. Houve maior risco de estresse entre os profissionais que realizam plantão noturno, maior risco de ansiedade em técnicos de enfermagem e em profissionais que trabalham no período diurno ou folguistas e depressão em técnicos de enfermagem. Conclusão: Os níveis de estresse, ansiedade e depressão encontrados no estudo foram baixos, o que difere do esperado e pode estar relacionado a fatores protetores como a satisfação e identificação com o trabalho. Se houver secções dentro do resumo devem estar a negrito e iniciar-se numa nova linha

Abstract Introduction: The Mobile Emergency Care Service - SAMU 192 is the pre-hospital mobile component instituted by the Ministry of Health in Brazil. With the COVID-19 pandemic, the service started to assist patients affected by the disease. This scenario brought great tension to the professionals, making it relevant to understand the psychological changes experienced by the team to provide adequate psychological assistance and well-being to them. Objective: To analyze levels of depression, anxiety, and stress in the SAMU 192 team. Methodology: Cross-sectional, exploratory-descriptive study with a quantitative approach. The shortened version of the Depression, Anxiety, and Stress Scale-21 (DASS-21) was applied to the SAMU 192 team, a four-point Likert scale that assesses levels of depression, anxiety, and stress. Results: Fifty-four SAMU 192 professionals participated in the research, accounting for 78.8% of the total team. The average age was 37.41 years, with the majority being female. Most professionals exhibited normal levels of depression, anxiety, and stress, however, cases of alterations represented 27.77%, 24.08%, and 18.51%, respectively. There was a higher risk of stress among professionals working night shifts, a higher risk of anxiety among nursing technicians and professionals working during the day or on rotating shifts, and depression among nursing technicians. Conclusion: The levels of stress, anxiety, and depression found in the study were low, which differs from expectations and may be related to protective factors such as job satisfaction and identification with work.

Resumen Introducción: El Servicio de Atención Móvil de Urgencia - SAMU 192 es el componente prehospitalario móvil instituido por el Ministerio de Salud en Brasil. Con la pandemia COVID-19, el servicio comenzó a atender a pacientes afectados por la enfermedad. Este escenario generó una gran tensión en los profesionales, lo que hizo relevante comprender las alteraciones psicológicas experimentadas por el equipo, con el fin de proporcionar una asistencia psicológica adecuada y garantizar su bienestar. Objetivo: Analizar los niveles de depresión, ansiedad y estrés en el equipo del SAMU 192. Metodología: Estudio transversal, exploratorio-descriptivo, con enfoque cuantitativo. Se aplicó la versión reducida de la escala de Depresión, Ansiedad y Estrés (DASS-21) en el equipo del SAMU 192, una escala tipo Likert de cuatro puntos que evalúa los niveles de depresión, ansiedad y estrés. Resultados: Participaron en la investigación 54 profesionales del SAMU 192, que representaron el 78,8% del equipo total. La edad promedio fue de 37,41 años, siendo la mayoría del sexo femenino. La mayoría de los profesionales presentaron niveles normales de depresión, ansiedad y estrés, sin embargo, los casos de alteraciones representaron el 27,77%, 24,08% y 18,51% respectivamente. Hubo un mayor riesgo de estrés entre los profesionales que trabajan en turnos nocturnos, un mayor riesgo de ansiedad en técnicos de enfermería y en profesionales que trabajan en el turno diurno o en días libres, y depresión en técnicos de enfermería. Conclusión: Los niveles de estrés, ansiedad y depresión encontrados en el estudio fueron bajos, lo que difiere de lo esperado y puede estar relacionado con factores protectores como la satisfacción e identificación con el trabajo.

Cross-cultural adaptation of the CALCULATE instrument into Brazilian Portuguese: pressure injury in intensive care / Adaptación transcultural del instrumento CALCULATE al portugués brasileño: lesión por presión en cuidados intensivos / Adaptação transcultural do instrumento CALCULATE para o português brasileiro: lesão por pressão em terapia intensiva

ABSTRACT Objective: To perform the cross-cultural adaptation of CALCULATE for Brazilian Portuguese. Method: A methodological study conducted from January to December 2021, divided into six stages: translation, synthesis, back-translation, expert committee with the application of the content validity index, pre-testing in 40 adult patients, and submission to the authors. The study took place in the intensive care units of a public tertiary teaching hospital in the interior of the state of São Paulo, Brazil. The original CALCULATE has eight risk assessment items and is stratified with a score of 0-3 (high risk) and 4-8 (very high risk). Results: After expert evaluation, the final content validity was 0.9. They suggested words and phrases that should undergo changes regarding textual equivalences, as well as definitions of acronyms and terminologies. In the pre-test, the items were assessed as suitable for understanding; only one item required additional explanation for adequacy. Conclusion: The cross-cultural adaptation of CALCULATE for Brazilian Portuguese was successfully performed, revealing a good content validity index, confirming the relevance and appropriateness of its items. CALCULATE is suitable for use in intensive care units and research and teaching centers.

RESUMEN Objetivo: Realizar la adaptación transcultural de CALCULATE al portugués brasileño. Método: Un estudio metodológico llevado a cabo de enero a diciembre de 2021, dividido en seis etapas: traducción, síntesis, retrotraducción, comité de expertos con aplicación del índice de validez de contenido, preprueba en 40 pacientes adultos y envío a las autoras. El estudio se realizó en las unidades de cuidados intensivos de un hospital público de enseñanza terciaria en el interior del estado de São Paulo, Brasil. El CALCULATE original tiene ocho ítems de evaluación (factores de riesgo) y se estratifica con un puntaje de 0-3 (alto riesgo) y 4-8 (riesgo muy alto). Resultados: Después de la evaluación de los expertos, la validez de contenido final fue de 0,9. Sugirieron palabras y frases que debían cambiar en cuanto a equivalencias textuales, así como definiciones de siglas y terminologías. En la preprueba, los ítems se evaluaron como adecuados para la comprensión; solo un ítem requirió una explicación adicional para su adecuación. Conclusión: Se realizó con éxito la adaptación transcultural de CALCULATE al portugués brasileño, revelando un buen índice de validez de contenido, confirmando la relevancia y adecuación de sus ítems. CALCULATE es adecuado para su uso en unidades de cuidados intensivos y centros de investigación y enseñanza.

RESUMO Objetivo: Realizar a adaptação transcultural do CALCULATE para o português brasileiro. Método: Estudo metodológico, desenvolvido de janeiro a dezembro de 2021 e dividido em seis etapas: tradução, síntese, retrotradução, comitê de especialistas com aplicação de índice de validade de conteúdo, pré-teste em 40 pacientes adultos e envio às autoras. O estudo ocorreu nas unidades de terapia intensiva em hospital público de ensino de nível terciário no interior do estado de São Paulo, Brasil. O CALCULATE original tem oito itens de avaliação (fatores de risco) e é estratificado com escore 0-3 (alto risco) e 4-8 (risco muito alto). Resultados: Após avaliação dos especialistas, a validade de conteúdo final foi de 0,9. Eles sugeriram palavras e frases que deveriam sofrer alterações quanto às equivalências textuais, assim como definições de siglas e terminologias. No pré-teste, os itens foram avaliados como adequados na compreensão; apenas um item precisou de explicação complementar para adequação. Conclusão: Foi realizada a adaptação transcultural do CALCULATE para o português do Brasil, a qual revelou ter bom índice de validade de conteúdo, sendo verificada a pertinência e relevância de seus itens. O CALCULATE está adequado para utilização em unidades de terapia intensiva e centros de pesquisa e ensino.

Nursing workload and severity of COVID-19 patients in the Intensive Care Unit / Carga de trabajo de enfermería y gravedad de los pacientes con COVID-19 en la Unidad de Cuidados Intensivos / Carga de trabalho de Enfermagem e gravidade de pacientes com COVID-19 na Unidade de Terapia Intensiva

ABSTRACT Objective: To evaluate the workload and severity of patients in the Intensive Care Unit (ICU) with COVID-19. Method: Cross-sectional, analytical study carried out in the ICU of a private hospital. All patients over the age of 18 with a diagnosis of COVID-19 admitted from September 2020 to June 2021 were included. Workload assessed by the Nursing Activities Score (NAS), and severity by the Sequential Organ Failure Assessment. Descriptive and inferential analyses were performed. Results: 217 patients were included, mostly men, mean age 62.41 years, white, obese, non-smokers and sedentary. The average NAS was 84.79. Staffing was in line with legislation and NAS. NAS was not associated with severity. Severity was associated with higher age, gender, comorbidities, sedentary lifestyle, time on mechanical ventilation, hospitalization and death. Conclusion: Workload was high and not associated with severity or outcomes. Severity was associated with demographic and clinical conditions. This study shows the importance of staff sizing, with a view to promoting safety and quality of care.

RESUMEN Objetivo: Evaluar la carga de trabajo y la gravedad de los pacientes de la Unidad de Cuidados Intensivos (UCI) con COVID-19. Método: Estudio transversal y analítico realizado en la UCI de un hospital privado. Se incluyeron todos los pacientes mayores de 18 años con diagnóstico de COVID-19 ingresados entre septiembre de 2020 y junio de 2021. Carga de trabajo evaluada mediante la Nursing Activities Score (NAS), y gravedad mediante la valoración secuencial de fallo orgánico. Se realizaron análisis descriptivos e inferenciales. Resultados: Se incluyeron 217 pacientes, en su mayoría hombres, edad media 62,41 años, raza blanca, obesos, no fumadores y sedentarios. El NAS medio era de 84,79. Los niveles de personal se ajustaban a la legislación y al NAS. El NAS no se asoció con la gravedad. La gravedad se asoció a mayor edad, sexo, comorbilidades, sedentarismo, tiempo de ventilación mecánica, hospitalización y muerte. Conclusión: La carga de trabajo fue elevada y no se asoció a la gravedad ni a los resultados. La gravedad se asoció a las condiciones demográficas y clínicas. Este estudio muestra la importancia del dimensionamiento del personal, con vistas a promover la seguridad y la calidad de los cuidados.

RESUMO Objetivos: Avaliar carga de trabalho e gravidade dos pacientes na Unidade de Terapia Intensiva (UTI) com COVID-19. Método: Estudo transversal, analítico realizado na UTI em hospital privado. Incluídos todos os pacientes maiores de 18 anos, com diagnóstico de COVID-19 admitidos de setembro de 2020 a junho de 2021. Carga de trabalho avaliado pelo Nursing Activities Score (NAS), e gravidade pelo Sequential Organ Failure Assessment. Realizado análises descritiva e inferencial. Resultados: Incluídos 217 pacientes, maioria homens, média de idade 62,41 anos, brancos, obesos, não tabagistas e sedentários. A média do NAS foi 84,79. O dimensionamento de pessoal estava em concordância com legislação e NAS. O NAS não foi associado a gravidade. Houve associação da gravidade com maior idade, sexo, comorbidades, sedentarismo, tempo de ventilação mecânica, internação e óbito. Conclusão: A carga de trabalho foi alta e não associada a gravidade e desfechos. A gravidade foi associada às condições demográficas e clínicas. Este estudo mostra a importância do dimensionamento de pessoal, com vistas à promoção da segurança e qualidade assistencial.

Biocellulose-based hydrogel dressing as a strategy for the management of chronic arterial wounds

Purpose: To evaluate using a biocellulose-based hydrogel as an adjuvant in the healing process of arterial ulcers. Methods: A prospective single group quasi-experimental study was carried out with chronic lower limb arterial ulcer patients. These patients received biocellulose-based hydrogel dressings and outpatient guidance on dressing and periodic reassessments. The primary outcomes were the ulcer-healing rate and product safety, which were assessed by ulcer area measured in photographic records of pre-treatment and posttreatment after 7, 30, and 60 days. Secondary outcomes were related to clinical assessment by the quality-of-life scores (SF-36 and EQ-5D) and pain, evaluated by the visual analogue scale (VAS). Results: Seventeen participants were included, and one of them was excluded. Six patients (37%) had complete wound healing, and all patients had a significant reduction in the ulcer area during follow-up (233.6mm2 versus 2.7mm2) and reduction on the score PUSH 3.0 (p < 0.0001). The analysis of the SF-36 and EQ-5D questionnaires showed a statistically significant improvement in almost all parameters analyzed and with a reduction of pain assessed by the VAS. Conclusions: The biocellulose-based hydrogel was safe and showed a good perspective to promoting the necessary conditions to facilitate partial or complete healing of chronic arterial ulcers within a 60-day follow-up. Quality of life and pain were positively affected by the treatment.

Growth hormone and heart failure: oxidative stress and energetic metabolism in rats.

Several evidences point for beneficial effects of growth hormone (GH) in heart failure (HF). Taking into account that HF is related with changes in myocardial oxidative stress and in energy generation from metabolic pathways, it is important to clarify whether GH increase or decrease myocardial oxidative stress and what is its effect on energetic metabolism in HF condition. Thus, this study investigated the effects of two different doses of GH on energetic metabolism and oxidative stress in myocardium of rats with HF. Male Wistar rats (n=25) were submitted to aortic stenosis (AS). The HF was evidenced by tachypnea and echocardiographic criteria around 28 weeks of AS. The rats were then randomly divided into three groups: (HF) with HF, treated with saline (0.9% NaCl); (HF-GH1), treated with 1 mk/kg/day recombinant human growth hormone (rhGH), and (HF-GH2) treated with 2 mg/kg/day rhGH. GH was injected, subcutaneously, daily for 2 weeks. A control group (sham; n=12), with the same age of the others rats was evaluated to confirm data for AS. HF had lower IGF-I (insulin-like growth factor-I) than sham-operated rats, and both GH treatments normalized IGF-I level. HF-GH1 animals had lower lipid hydroperoxide (LH), LH/total antioxidant substances (TAS) and glutathione-reductase than HF. Glutathione peroxidase (GSH-Px), hydroxyacyl coenzyme-A dehydrogenase, lactate dehydrogenase(LDH) were higher in HF-GH1 than in HF. HF-GH2 compared with HF, had increased LH/TAS ratio, as well as decreased oxidized glutathione and LDH activity. Comparing the two GH doses, GSH-Px, superoxide dismutase and LDH were lower in HF-GH2 than in HF-GH1. In conclusion, GH effects were dose-dependent and both tested doses did not aggravate the heart dysfunction. The higher GH dose, 2 mg/kg exerted detrimental effects related to energy metabolism and oxidative stress. The lower dose, 1mg/kg GH exerted beneficial effects enhancing antioxidant defences, reducing oxidative stress and improving energy generation in myocardium of rats with heart failure.