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Proteomics ; 14(12): 1543-6, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24723484

RESUMO

Yellow lupin (Lupinus luteus L.) is a legume crop containing a large amount of protein in its seeds. In this study, we constructed a seed-protein catalog to provide a foundation for further study of the seeds. A total of 736 proteins were identified in 341 2DE spots by nano-LC-MS/MS. Eight storage proteins were found as multiple spots in the 2DE gels. The 736 proteins correspond to 152 unique proteins as shown by UniRef50 clustering. Sixty-seven of the 152 proteins were associated with KEGG-defined pathways. Of the remaining proteins, 57 were classified according to a GO term. The functions of the remaining 28 proteins have yet to be determined. This is the first yellow lupin seed-protein catalog, and it contains considerably more data than previously reported for white lupin (L. albus L.).


Assuntos
Lupinus/metabolismo , Proteínas de Plantas/metabolismo , Proteoma/metabolismo , Proteômica/métodos , Sementes/metabolismo , Cromatografia Líquida , Eletroforese em Gel Bidimensional , Proteínas de Plantas/genética , Sementes/química , Espectrometria de Massas em Tandem
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