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1.
Rocz Akad Med Bialymst ; 47: 40-57, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12533947

RESUMO

Zoosporic and conidial fungi species and environmental factors in 5 various sites of the Podlasie stretch of the river Bug were studied. Samples of water were collected in October 1999 and April 2000 for hydrochemical analysis and the order to determine the number of fungal species. Buckwheat-seeds, hemp-seeds, cellophane and snake exuviae were used as bait during the exposure in laboratory. Sixty-four zoosporic fungus species and forty-six conidial species were found in various sites of the Podlasie stretch of the river Bug. 8 species were recorded for the first time from Poland.


Assuntos
Conidiobolus/isolamento & purificação , Esporos Fúngicos/isolamento & purificação , Microbiologia da Água , Poluição da Água/análise , Ecossistema , Monitoramento Ambiental , Poluentes Ambientais/análise , Água Doce/microbiologia , Humanos , Polônia , Estudos de Amostragem
2.
Rocz Akad Med Bialymst ; 47: 58-79, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12533948

RESUMO

Zoosporic and conidial fungi species and environmental factors in 10 various sites of River Narew in the Narew National Park were studied. Samples of water were collected in autumn 1999 and spring 2000 for hydrochemical analysis and the order to determine the number of fungal species. Buckwheat-seeds, hemp-seeds, cellophane and snake exuviae were used as bait during the exposure in laboratory. 198 lower aquatic fungus species were found in various sites of River Narew. 13 species were recorded for the first time from Poland.


Assuntos
Conidiobolus/isolamento & purificação , Poluição Ambiental/análise , Esporos Fúngicos/isolamento & purificação , Microbiologia da Água , Ecossistema , Monitoramento Ambiental , Agricultura Florestal , Água Doce/microbiologia , Humanos , Polônia , Estudos de Amostragem
3.
Cell Mol Biol Lett ; 8(1): 19-24, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-12655352

RESUMO

Three acidic glycosidases: beta-galactosidase (beta-GAL, EC 3.2.1.23), alpha-neuraminidase (NEUR, sialidase, EC 3.2.1.18), N-acetylaminogalacto-6-sulfate sulfatase (GALNS, EC 3.1.6.4) and serine carboxypepidase cathepsin A (EC 3.4.16.1) form a functional high molecular weight complex in the lysosomes. The major constituent of this complex is cathepsin A, the so-called "lysosomal protective protein" (PPCA). By forming a multienzyme complex, it protects the glycosidases from rapid intralysosomal proteolysis, and it is also required for the intracellular sorting and proteolytic processing of their precursors. In man, a deficiency of cathepsin A leads to a combined deficiency of beta-GAL and NEUR activities, called "galactosialidosis". Multiple mutations identified in the cathepsin A gene are the molecular basis of this lysosomal storage disease. This review describes the structural organization of the lysosomal high molecular weight multienzyme complex and the importance of the protective protein/cathepsin A in physiology and pathology.


Assuntos
Lisossomos/enzimologia , Complexos Multienzimáticos/metabolismo , Catepsina A/química , Catepsina A/genética , Catepsina A/metabolismo , Condroitina Sulfatases/genética , Condroitina Sulfatases/metabolismo , Humanos , Doenças por Armazenamento dos Lisossomos/enzimologia , Doenças por Armazenamento dos Lisossomos/genética , Peso Molecular , Complexos Multienzimáticos/química , Complexos Multienzimáticos/genética , Mutação , Neuraminidase/química , Neuraminidase/metabolismo , beta-Galactosidase/química , beta-Galactosidase/metabolismo
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