RESUMO
Small Gram-negative cocco-bacilli resembling Brucella strains have been reported from marine mammals since the mid-1990s. Their placement in the genus Brucella has been supported by the following characteristics: they are aerobic, non-motile and catalase-positive, do not produce acid from carbohydrates and have a DNA-DNA relatedness value of >77% with the six established members of the genus. Twenty-eight European isolates of the genus Brucella from marine mammals were distinguished from the six recognized species by their pattern of utilization of eleven substrates in oxidative metabolism tests and phage lysis. The 28 strains could be further separated into two groups with cetaceans and seals as their respective preferred hosts on the basis of molecular methods and on differences in the metabolism of l-arabinose, d-galactose and d-xylose. The names Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. are proposed for the isolates from cetaceans and seals, respectively. The type strain of Brucella ceti sp. nov. is NCTC 12891T (=BCCN 94-74T) and the type strain of Brucella pinnipedialis sp. nov. is NCTC 12890T (=BCCN 94-73T).
Assuntos
Brucella/classificação , Caniformia/microbiologia , Cetáceos/microbiologia , Animais , Técnicas de Tipagem Bacteriana , Brucella/genética , Brucella/metabolismo , Brucella/fisiologia , Genótipo , Lisogenia , Hibridização de Ácido Nucleico , Oxirredução , Fenótipo , Especificidade da EspécieRESUMO
We report on the isolation of two species of Actinobaculum from blood culture of a patient with chronic renal failure. The two isolates were distinct with regard to their morphological and biochemical characteristics. Subsequent 16S rRNA gene sequencing classified the two species as Actinobaculum schaalii and A. urinale.
Assuntos
Actinomycetaceae/isolamento & purificação , Infecções por Actinomycetales/microbiologia , Falência Renal Crônica/complicações , Actinomycetaceae/classificação , Actinomycetaceae/genética , Idoso , Sangue/microbiologia , Meios de Cultura , DNA Bacteriano/análise , DNA Ribossômico/análise , Genes de RNAr , Humanos , Masculino , Dados de Sequência Molecular , Fenótipo , RNA Ribossômico 16S , Análise de Sequência de DNARESUMO
AFLP was used to analyze the genetic diversity among Ochrobactrum strains. AFLP patterns showed a great genomic variability that separated the samples into three distinct clusters. Ochrobactrum intermedium was found to be closely related to Brucella abortus S99.
Assuntos
Ochrobactrum/genética , Técnicas de Tipagem Bacteriana , Genes Bacterianos , Genótipo , Técnicas de Amplificação de Ácido Nucleico , Ochrobactrum/classificação , Filogenia , Polimorfismo Genético , Especificidade da EspécieRESUMO
Four chromogenic urine culture media were compared to culture on blood agar, MacConkey agar, and CLED (cysteine-, lactose-, and electrolyte-deficient) agar for detection of uropathogens in 1,200 urine specimens. After 2 nights of incubation, 96% of all isolates were recovered on blood agar, 96% were recovered on CLED agar, 92% were recovered on CPS ID2, 96% were recovered on CHROMagar Orientation from BBL, 95% were recovered on CHROMagar Orientation from The CHROMagar Company, and 95% were recovered on Chromogenic UTI Medium.