Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 49
Filtrar
1.
Pol Merkur Lekarski ; 52(1): 5-9, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38518226

RESUMO

OBJECTIVE: Aim: To analyse onconeural antibodies in the blood serum of breast cancer patients without neurological symptoms.. PATIENTS AND METHODS: Materials and Methods: The study included 48 women with breast cancer. Paraneoplastic Neurologic Syndromes 12 Ag (IgG) Euroline by EUROIMMUN test was used to determine onconeural antibodies: anti-Hu, anti-Yo, anti-Ri, anti-CV2, anti-Ma/anti-Ta, anti-amphiphysin, anti-recoverin, anti-SOX1, anti-tytin, anti-zic4, anti-GAD65 and anti-Tr (DNER). RESULTS: Results: The conducted analysis revealed the presence of onconeural antibodies such as: anti-recoverin, anti-CV2, anti-Zic4, anti-SOX1, anti-MA2/Ta and antititin in blood serum of women with breast cancer. CONCLUSION: Conclusions: Further analysis may allow the assessment of the possible clinical usefulness of these determinations.


Assuntos
Neoplasias da Mama , Síndromes Paraneoplásicas do Sistema Nervoso , Humanos , Feminino , Prevalência , Síndromes Paraneoplásicas do Sistema Nervoso/diagnóstico , Autoanticorpos
2.
Pol Merkur Lekarski ; 50(298): 232-236, 2022 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-36086981

RESUMO

Endometriosis is an inflammatory estrogen-dependent gynecological disease characterized by the presence of endometrial tissue outside the uterine cavity. An important role in the pathogenesis of this disease is played by disorders of the immune system involving chemokines and their receptors, including the CXCL8-CXCR1/ 2 system. AIM: The aim of the study was to assess the concentration of the CXCL8 chemokine and its CXCR1 and CXCR2 receptors in the peritoneal fluid of women with endometriosis. MATERIALS AND METHODS: The study included 32 women aged 21 to 47 years with diagnosed endometriosis and a control group of 8 healthy women aged 21 to 40 years. The material for the research was the peritoneal fluid collected during the laparoscopic procedure. The concentration of chemokines was determined by ELISA tests. RESULTS: The conducted studies showed that the concentration of the CXCL8 chemokine was significantly higher in the peritoneal fluid of the studied women and depended on the clinical advancement of the disease. CONCLUSIONS: Changes in the concentration of the CXCL8 chemokine in the peritoneal fluid of women with endometriosis may indicate impaired immune response and indicate an inflammatory process within the peritoneal cavity. The demonstrated relationship between the concentration of CXCL8 and the stages of clinical advancement indicates a significant role of this chemokine in the development of the disease.


Assuntos
Líquido Ascítico/química , Endometriose , Interleucina-8/análise , Receptores de Interleucina-8A/análise , Receptores de Interleucina-8B/análise , Quimiocinas , Endometriose/imunologia , Feminino , Humanos , Interleucina-8/fisiologia
3.
Pol Merkur Lekarski ; 49(291): 227-230, 2021 06 16.
Artigo em Inglês | MEDLINE | ID: mdl-34218245

RESUMO

According to the data from November 29 2020, the SARS-CoV-2 coronavirus was responsible for 61 866 635 cases of infections and 1 448 990 deaths worldwide, and the number is still growing rapidly. The main problem is the rapid increase in the number of patients with pneumonia complicated by respiratory failure. In the treatment of COVID- 19 patients, a significant effect of convalescent plasma (CP) therapy from convalescent patients with SARS-CoV-2 IgG neutralizing antibodies is indicated. After this procedure, the total duration of the infection was shortened and the clinical condition improved faster than in patients who did not receive this form of therapy. The aim of the study was to explain the cause disqualifying women with anti-leucocyte anitibodies as CP plasma donors for COVID-19 patients. However, according to the literature, 2% of patients who received plasma from convalescents developed Transfusion Related Acute Lung Injury (TRALI). The most common causes of TRALI are anti-leukocyte antibodies directed against Human Leukocyte Antigens (HLA) class I and II and against Human Neutrophil Antigens (HNA). Therefore, patients with COVID-19 may only be transfused with plasma from convalescent women with a history of pregnancy after testing negative for anti-leucocyte antibodies in the pre-plasmapheresis blood sample.


Assuntos
COVID-19 , Infecções por Coronavirus , Complicações Infecciosas na Gravidez , Anticorpos Antivirais , COVID-19/terapia , Feminino , Humanos , Imunização Passiva , Gravidez , SARS-CoV-2 , Soroterapia para COVID-19
4.
Arch Gynecol Obstet ; 297(6): 1495-1501, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29572748

RESUMO

PURPOSE: The purpose of the work was to evaluate possible associations between the complement components C1q, mannose-binding lectin (MBL) and C1 inhibitor (C1INH) with pathogenesis of endometriosis. METHODS: Concentrations of C1q, MBL and C1INH were measured by ELISA in peritoneal fluid (PF) in 80 women with or without endometriosis. RESULTS: Significantly higher PF levels of C1q, MBL and C1INH in women with endometriosis compared to control group were observed (p < 0.0001). A higher concentration of the studied parameter was found in PF of women at the early stage of the disease, as compared to women with advanced endometriosis (p < 0.0001). CONCLUSIONS: Our research suggests that in the peritoneal cavity in women with endometriosis there are abnormal regulations of both the classical and lectin pathways of the complement system. This can suggest impairments in purification of peritoneal cavity from ectopic endometrial cells and augmented local inflammation in endometriosis patients.


Assuntos
Líquido Ascítico/metabolismo , Proteína Inibidora do Complemento C1/metabolismo , Complemento C1q/metabolismo , Endometriose/metabolismo , Endometriose/patologia , Lectina de Ligação a Manose/metabolismo , Adulto , Estudos de Casos e Controles , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Lectinas , Pessoa de Meia-Idade
5.
J Clin Lab Anal ; 30(6): 831-837, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27018977

RESUMO

BACKGROUND: The most important proinflammatory cytokine is interleukin (IL)-1ß, however its precursor, prointerleukin-1ß (proIL-1ß), can also potentiate inflammatory state. The aim of this study was to explore the involvement of proIL-1ß in pathogenesis of endometriosis. For this purpose, we evaluated concentrations of proIL-1ß, IL-1ß, and soluble IL-1 receptor type 2 (sIL-1R2) in peritoneal fluid (PF) and macrophage culture medium of women with endometriosis. METHODS: PF from 55 women with and without endometriosis was collected during laparoscopy. Peritoneal macrophages were cultured in basal and stimulated with lipopolysaccharide (LPS) conditions. Concentrations of cytokines were measured with enzyme-linked immunosorbent assays (ELISA). RESULTS: PF proIL-1ß and IL-1ß levels in endometriosis women were higher than in the control. Higher basal and stimulated macrophage secretion of cytokines in endometriosis patients than in the control was observed. However, in endometriosis, there was a higher level of proIL-1ß than for the mature molecule. Additionally, lower PF and macrophages culture medium sIL-1R2 levels were observed in women with endometriosis. CONCLUSIONS: Abnormal proIL-1ß concentration in PF and higher macrophage secretion can escalate peritoneal inflammation and endometriosis formation. The results are presented as a total IL-1ß, which is a sum of proIL-1ß  and IL-1ß, and we believe that it reflects the actual cytokine production. The imbalance among all studied cytokines in endometriosis may be linked with an ability to transform acute inflammation to the chronic inflammation.


Assuntos
Líquido Ascítico/metabolismo , Citocinas/metabolismo , Endometriose/diagnóstico , Endometriose/metabolismo , Precursores de Proteínas/metabolismo , Adulto , Análise de Variância , Técnicas de Cultura de Células , Endometriose/patologia , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Lipopolissacarídeos/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Adulto Jovem
6.
Cytokine ; 76(2): 117-122, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26315533

RESUMO

The aim of this study was to evaluate differences in levels of serum and monocyte derived interleukin (IL)-1, IL-6 and neopterin (NPT) in women with normal or abnormal menstrual cycles and women with endometriosis. The women participating in this study were divided into 4 groups: 25 women with normal menstrual cycle; 25 women taking oral contraception (OC); 20 postmenopausal women and 25 endometriosis patients. IL-1beta, IL-6 and NPT levels in serum and monocyte culture media were measured with ELISA methods. The data collected showed the lowest serum NPT levels in women with follicular menstrual cycles. The levels of both types of interleukins in serum were the lowest in women using OC. In contrast, the highest concentrations of all cytokines were found in the serum of women with endometriosis. The lowest monocyte activity was observed in women with a follicular menstrual cycle phase and the highest in endometriosis. Monocytes from women using OC secreted similar amounts of cytokines to the cells during the follicular menstrual cycle phase. Changes occurring at the time of contraception, after menopause and during endometriosis, are followed by changed proinflammatory monocyte activity, which is associated with different secretion of cytokines. OC can inhibit inflammatory monocyte properties. Lower serum concentration of cytokines compared to cell secretion may suggest some control mechanisms of monocyte activity.


Assuntos
Endometriose/sangue , Endometriose/imunologia , Interleucina-1/sangue , Interleucina-6/sangue , Ciclo Menstrual/sangue , Ciclo Menstrual/imunologia , Monócitos/imunologia , Neopterina/sangue , Adulto , Células Cultivadas , Anticoncepcionais Orais , Feminino , Humanos , Inflamação/sangue , Inflamação/etiologia , Inflamação/imunologia , Menopausa/imunologia , Pessoa de Meia-Idade , Monócitos/fisiologia , Adulto Jovem
7.
Diagnostics (Basel) ; 14(9)2024 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-38732363

RESUMO

One of the greatest challenges in modern gynecological oncology is ovarian cancer. Despite the numerous studies currently being conducted, it is still sometimes detected at late clinical stages, where the prognosis is unfavorable. One significant contributing factor is the absence of sensitive and specific parameters that could aid in early diagnosis. An ideal screening test, in view of the low incidence of ovarian cancer, should have a sensitivity of greater than 75% and a specificity of at least 99.6%. To enhance sensitivity and specificity, diagnostic panels are being created by combining individual markers. The drive to develop better screening tests for ovarian cancer focuses on modern diagnostic methods based on molecular testing, which in turn aims to find increasingly effective biomarkers. Currently, researchers' efforts are focused on the search for a complementary parameter to those most commonly used that would satisfactorily enhance the sensitivity and specificity of assays. Several biomarkers, including microRNA molecules, autoantibodies, cDNA, adipocytokines, and galectins, are currently being investigated by researchers. This article reviews recent studies comparing the sensitivity and specificity of selected parameters used alone and in combination to increase detection of ovarian cancer at an early stage.

8.
Nutrients ; 16(8)2024 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-38674891

RESUMO

The complex and multi-stage processes of carcinogenesis are accompanied by a number of phenomena related to the potential involvement of various chemopreventive factors, which include, among others, compounds of natural origin such as flavonols. The use of flavonols is not only promising but also a recognized strategy for cancer treatment. The chemopreventive impact of flavonols on cancer arises from their ability to act as antioxidants, impede proliferation, promote cell death, inhibit angiogenesis, and regulate the immune system through involvement in diverse forms of cellular death. So far, the molecular mechanisms underlying the regulation of apoptosis, autophagy, necroptosis, pyroptosis, ferroptosis, and cuproptosis occurring with the participation of flavonols have remained incompletely elucidated, and the results of the studies carried out so far are ambiguous. For this reason, one of the therapeutic goals is to initiate the death of altered cells through the use of quercetin, kaempferol, myricetin, isorhamnetin, galangin, fisetin, and morin. This article offers an extensive overview of recent research on these compounds, focusing particularly on their role in combating cancer and elucidating the molecular mechanisms governing apoptosis, autophagy, necroptosis, pyroptosis, ferroptosis, and cuproptosis. Assessment of the mechanisms underlying the anticancer effects of compounds in therapy targeting various types of cell death pathways may prove useful in developing new therapeutic regimens and counteracting resistance to previously used treatments.


Assuntos
Apoptose , Autofagia , Ferroptose , Flavonóis , Necroptose , Neoplasias , Piroptose , Humanos , Flavonóis/farmacologia , Neoplasias/tratamento farmacológico , Neoplasias/patologia , Ferroptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Piroptose/efeitos dos fármacos , Apoptose/efeitos dos fármacos , Necroptose/efeitos dos fármacos , Animais , Morte Celular/efeitos dos fármacos
9.
Oncol Lett ; 27(6): 260, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38646491

RESUMO

The C-X-C motif chemokine ligand 8 (CXCL8)-C-X-C chemokine receptor (CXCR)1/2 signalling axis is among numerous mechanisms which stimulate the immune system to defend against tumour growth and influence the tumour microenvironment to promote tumour growth. This pathway plays an important role in the development of a number of cancers including breast cancer (BC). The aim of the present study was to analyse the levels of the chemokine CXCL8 and its receptors, CXCR1 and CXCR2, in the serum of female patients with invasive BC and to assess the expression of these parameters at the mRNA level, considering molecular subtypes and degrees of cancer malignancy. The study group consisted of 62 patients with histopathologically confirmed invasive BC. The control group consisted of 18 patients with histopathologically confirmed fibroadenoma, a benign breast tumour. The levels of CXCL8, CXCR1 and CXCR2 were determined by sandwich ELISA using the CLOUD-CLONE ELISA kit. CXCL8, CXCR1 and CXCR2 transcript levels were analysed using reverse transcription-quantitative PCR. Results showed that serum CXCL8 levels in female patients with invasive BC were significantly higher compared with those in the control group (P<0.05). In addition, significantly elevated CXCR1 levels were observed in luminal B human epidermal growth factor receptor 2+ carcinoma compared with those in the control group. Analysis of CXCL8 in the serum of female patients with BC showed a statistically significant difference between clinical stage G1 and G2 (P<0.05), G2 and G3 (P<0.01), and G1 and G3 (P<0.0001). On the other hand, the analysis of CXCR1 and CXCR2 levels in the serum of the patients revealed a statistically significant difference between G2 and G3 (P<0.05). The current study showed that abnormalities in the immune response involving the CXCL8-CXCR1/2 signalling axis in patients with invasive BC are involved in the development of these tumours. Moreover, the demonstrated severity of changes occurring at protein level may suggest the potential usefulness of their determination as potential diagnostic markers in the clinic.

10.
Nutrients ; 15(13)2023 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-37447264

RESUMO

The consumption of foods that are rich in phenolic compounds has chemopreventive effects on many cancers, including breast cancer, ovarian cancer, and endometrial cancer. A wide spectrum of their health-promoting properties such as antioxidant, anti-inflammatory, and anticancer activities, has been demonstrated. This paper analyzes the mechanisms of the anticancer action of selected common flavonols, including kemferol, myricetin, quercetin, fisetin, galangin, isorhamnetin, and morin, in preclinical studies, with particular emphasis on in vitro studies in gynecological cancers and breast cancer. In the future, these compounds may find applications in the prevention and treatment of gynecological cancers and breast cancer, but this requires further, more advanced research.


Assuntos
Neoplasias da Mama , Flavonoides , Humanos , Feminino , Flavonoides/farmacologia , Flavonoides/uso terapêutico , Flavonóis/farmacologia , Quercetina/farmacologia , Antioxidantes/farmacologia , Neoplasias da Mama/tratamento farmacológico
11.
Biomedicines ; 11(6)2023 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-37371799

RESUMO

MicroRNAs are non-coding transcripts that, thanks to the ability to regulate the mRNA of target genes, can affect the expression of genes encoding tumor suppressors and oncogenes. They can control many important cellular processes, including apoptosis, differentiation, growth, division, and metabolism. Therefore, miRNAs play an important role in the development of many cancers, including gynecological cancers. Ovarian cancer, endometrial cancer, cervical cancer, and vulvar cancer are the most common cancers in women and are a frequent cause of death. The heterogeneity of the pathogenesis of these gynecological diseases makes the diagnostic process a significant obstacle for modern medicine. To date, many studies have been carried out, in which particular attention has been paid to the molecular pathomechanism of these diseases, with particular emphasis on miRNAs. To date, the changed profile of many miRNAs, which influenced the promotion of proliferation, migration, invasion processes and the simultaneous inhibition of programmed cell death, has been proven many times. Detailed understanding of the molecular effects of miRNAs in the above-mentioned gynecological cancers will enable the development of potential predictive and prognostic biomarkers, as well as the optimization of the diagnostic process.

12.
Diagnostics (Basel) ; 13(10)2023 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-37238197

RESUMO

Ovarian cancer is one of the most serious challenges in modern gynaecological oncology. Due to its non-specific symptoms and the lack of an effective screening procedure to detect the disease at an early stage, ovarian cancer is still marked by a high mortality rate among women. For this reason, a great deal of research is being carried out to find new markers that can be used in the detection of ovarian cancer to improve early diagnosis and survival rates of women with ovarian cancer. Our study focuses on presenting the currently used diagnostic markers and the latest selected immunological and molecular parameters being currently investigated for their potential use in the development of new diagnostic and therapeutic strategies.

13.
Cells ; 12(8)2023 04 09.
Artigo em Inglês | MEDLINE | ID: mdl-37190027

RESUMO

Due to their multidirectional influence, adipocytokines are currently the subject of numerous intensive studies. Significant impact applies to many processes, both physiological and pathological. Moreover, the role of adipocytokines in carcinogenesis seems particularly interesting and not fully understood. For this reason, ongoing research focuses on the role of these compounds in the network of interactions in the tumor microenvironment. Particular attention should be drawn to cancers that remain challenging for modern gynecological oncology-ovarian and endometrial cancer. This paper presents the role of selected adipocytokines, including leptin, adiponectin, visfatin, resistin, apelin, chemerin, omentin and vaspin in cancer, with a particular focus on ovarian and endometrial cancer, and their potential clinical relevance.


Assuntos
Neoplasias do Endométrio , Neoplasias Ovarianas , Humanos , Feminino , Adipocinas/fisiologia , Tecido Adiposo/fisiologia , Adiponectina , Microambiente Tumoral
14.
Ginekol Pol ; 94(6): 435-441, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36929784

RESUMO

OBJECTIVES: Ovarian cancer is one of the gynecological cancers that have the worst prognosis. The expression of the proteins from the IAP family (inhibitor of apoptosis protein), including survivin, is observed in many types of cancer. The aim of the study was to evaluate survivin at the mRNA level in tumors and the protein concentration in the serum and peritoneal fluid of patients with serous ovarian cancer in order to assess the relationship between the concentration of survivin and the histological subtypes of cancer. MATERIAL AND METHODS: The study group consisted of 55 women, including patients with serous ovarian cancer (n = 30, nine low-grade serous carcinoma LGSC, 21 high-grade serous carcinoma HGSC), serous cysts (n = 10) and the control group (n = 15). The concentration of protein in the peritoneal fluid and serum was assessed using ELISA tests. The expression of survivin gene BIRC5 in the tumors was assessed using the RT-qPCR method. RESULTS: The data that was obtained indicated that the concentration of survivin was higher in the serum of the women with serous ovarian cancer compared those that had benign tumors (p < 0.05) and the control group (p < 0.001). The survivin concentration was also higher in both the serum and peritoneal fluid in the HGSC group compared to the LGSC group (p < 0.001). The mRNA level was highest in the HGSC group, and there was a statistically significant difference compared to those in the benign tumor group and HGSC group ( p < 0.05). CONCLUSIONS: The observed changes prove that the expression level increases significantly in HGSC in both the protein and mRNA levels. Based on these findings, it can be assumed that assessing this parameter could be a useful additional indicator of the progression and differentiation of this type of cancer. However, this requires further research in a larger group of patients and possibly in other types of ovarian cancer.


Assuntos
Cistadenocarcinoma Seroso , Neoplasias Ovarianas , Humanos , Feminino , Survivina , Líquido Ascítico , RNA Mensageiro , Neoplasias Ovarianas/patologia , Carcinoma Epitelial do Ovário , Cistadenocarcinoma Seroso/metabolismo , Proteínas Inibidoras de Apoptose/genética
15.
Pol Merkur Lekarski ; 32(188): 111-5, 2012 Feb.
Artigo em Polonês | MEDLINE | ID: mdl-22590915

RESUMO

Systemic lupus erythematosus (SLE) is a common autoimmune disease of unknown etiology that frequently affects women of childbearing age. SLE is associated with impairments in immune system, which is a consequence of increased production of various autoantibodies. This disease is still a major diagnostic and therapeutic problem. Some hope to explain the complex etiopathogenesis of the disease creates a monitoring function of the immune system. A chance for patients are biologically targeted therapies that affect the critical stages of the disease and autoimmune inflammatory processes.


Assuntos
Lúpus Eritematoso Sistêmico/diagnóstico , Lúpus Eritematoso Sistêmico/terapia , Adulto , Feminino , Humanos , Lúpus Eritematoso Sistêmico/imunologia
16.
J Clin Med ; 11(5)2022 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-35268340

RESUMO

The extracellular matrix is a structure composed of many molecules, including fibrillar (types I, II, III, V, XI, XXIV, XXVII) and non-fibrillar collagens (mainly basement membrane collagens: types IV, VIII, X), non-collagenous glycoproteins (elastin, laminin, fibronectin, thrombospondin, tenascin, osteopontin, osteonectin, entactin, periostin) embedded in a gel of negatively charged water-retaining glycosaminoglycans (GAGs) such as non-sulfated hyaluronic acid (HA) and sulfated GAGs which are linked to a core protein to form proteoglycans (PGs). This highly dynamic molecular network provides critical biochemical and biomechanical cues that mediate the cell-cell and cell-matrix interactions, influence cell growth, migration and differentiation and serve as a reservoir of cytokines and growth factors' action. The breakdown of normal ECM and its replacement with tumor ECM modulate the tumor microenvironment (TME) composition and is an essential part of tumorigenesis and metastasis, acting as key driver for malignant progression. Abnormal ECM also deregulate behavior of stromal cells as well as facilitating tumor-associated angiogenesis and inflammation. Thus, the tumor matrix modulates each of the classically defined hallmarks of cancer promoting the growth, survival and invasion of the cancer. Moreover, various ECM-derived components modulate the immune response affecting T cells, tumor-associated macrophages (TAM), dendritic cells and cancer-associated fibroblasts (CAF). This review article considers the role that extracellular matrix play in breast cancer. Determining the detailed connections between the ECM and cellular processes has helped to identify novel disease markers and therapeutic targets.

17.
Oncol Lett ; 24(6): 465, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36406181

RESUMO

Ovarian cancer is a gynecological neoplasm that can be found in women, which, due to diagnostic difficulties, is often detected at advanced stages when treatment becomes a significant problem. Moreover, in a number of cases there is a cancer recurrence and resistance to standard chemotherapy treatment. It has been suggested that cancer stem cells (CSCs) that were not eradicated during therapy may be responsible for this. For this reason, effective therapeutic methods eliminating CSCs are being studied, such as therapy targeting CSCs markers. In addition, numerous studies have also drawn attention to the usefulness of CSCs markers in predicting disease progression and assessing patient's prognosis as well as their importance in the development of treatment resistance. The present review presented research on selected CSCs markers, which may be of significant prognostic and therapeutic importance in ovarian cancer.

18.
Mol Med Rep ; 25(5)2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35293602

RESUMO

The incidence of ovarian cancer is increasing, particularly throughout the highly developed countries, while this cancer type remains a major diagnostic and therapeutic challenge. The currently poorly recognized lectins called galectins have various roles in interactions occurring in the tumor microenvironment. Galectins are involved in tumor­associated processes, including the promotion of growth, adhesion, angiogenesis and survival of tumor cells. Results of research studies performed so far point to a complex role of galectins­1, 3, ­7, ­8 and ­9 in carcinogenesis of ovarian cancer and elucidation of the mechanisms may contribute to novel forms of therapies targeting the proteins. In particular, it appears important to recognize the reasons for changes in expression of galectins. Galectins also appear to be a useful diagnostic and prognostic tool to evaluate tumor progression or the efficacy of therapies in patients with ovarian cancer, which requires further study.


Assuntos
Galectinas , Neoplasias Ovarianas , Carcinogênese , Carcinoma Epitelial do Ovário , Galectinas/metabolismo , Humanos , Neovascularização Patológica/diagnóstico , Neovascularização Patológica/genética , Neoplasias Ovarianas/diagnóstico , Neoplasias Ovarianas/terapia , Microambiente Tumoral
19.
Mol Med Rep ; 26(4)2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35920183

RESUMO

Understanding the relationship between the coexistence of inflammatory and neoplastic processes in ovarian cancer, particularly those involving chemokines and their receptors, may help to elucidate the involvement of the studied parameters in tumor pathogenesis and could lead to improved clinical applications. Therefore, the present study aimed to analyze the levels of C­X­C motif chemokine ligand 8 (CXCL8), and its receptors C­X­C chemokine receptor (CXCR)1 and CXCR2, in the serum and peritoneal fluid of women with ovarian cancer, and to evaluate the association between the expression of these parameters in tumor tissue and patient characteristics, particularly the degree of histological differentiation. The study group included women with ovarian cancer diagnosed with serous cystadenocarcinoma International Federation of Gynecology and Obstetrics stage IIIc and a control group, which consisted of women who were diagnosed with a benign lesion (serous cystadenoma). The transcript levels of CXCL8, CXCR1 and CXCR2 were evaluated using reverse transcription­quantitative PCR (RT­qPCR). The quantitative analysis was carried out using the LightCycler® 480 System and GoTaq® 1­Step RT­qPCR System, according to the manufacturers' instructions. The concentration of CXCL8 in serum and peritoneal fluid was determined using a Human Interleukin­8 ELISA kit, and the concentrations of CXCR1 and CXCR2 were determined using the CLOUD­CLONE ELISA kit. Local and systemic disturbances in immune and inflammatory responses involving the CXCL8 chemokine and its receptors indicated the involvement of these studied parameters in the pathogenesis of ovarian cancer. Immunoregulation of the CXCL8­CXCR1 system may influence the course of the inflammatory process accompanying ovarian cancer development, which may result in the identification of novel clinical applications; however, further studies are required.


Assuntos
Interleucina-8 , Neoplasias Ovarianas , Receptores de Interleucina-8A , Receptores de Interleucina-8B , Líquido Ascítico/metabolismo , Feminino , Humanos , Interleucina-8/genética , Interleucina-8/metabolismo , Neoplasias Ovarianas/genética , Neoplasias Ovarianas/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Receptores de Interleucina-8A/genética , Receptores de Interleucina-8A/metabolismo , Receptores de Interleucina-8B/genética , Receptores de Interleucina-8B/metabolismo
20.
Ginekol Pol ; 82(11): 822-6, 2011 Nov.
Artigo em Polonês | MEDLINE | ID: mdl-22384614

RESUMO

OBJECTIVES: Malignant tumors of the ovary and uterus remain to be a diagnostic and therapeutic problem in Poland, mainly due to the lack of effective diagnosis of their early stages. There is a relation between an impaired immune system, especially the process of inflammation and the pathogenesis of these tumors. The aim of the study was to assess the concentration of calprotectin--a inflammation marker in the serum of women with ovarian or uterine cancer. MATERIAL AND METHODS: The study group included 96 women, aged 21 to 72 (mean age: 46.7 +/- 13.6 years) with the diagnosed and histologically confirmed ovarian or uterine tumor The control group consisted of 30 women aged 24-60 (mean age 45.6 +/- 8.9 years), showing no pathological disorders or any inflammations of the reproductive system. The concentration of calprotectin was evaluated with the use of the immunoenzymatic method ELISA using the Calprotectin ELISA (serum) kit by DRG Instruments (Germany). RESULTS: In serum of women with tumors the calprotectin level was significantly higher comparing to the control group (p < 0.0001). The highest calprotectin levels in women with ovarian cancer (mean +/- SD: 231.84 +/- 13.74 ng/ ml) and uterine cancer (mean +/- SD: 166.23 +/- 13.36 ng/ml) were observed and were significantly higher comparing to women with ovarian serous adenomas (mean +/- SD: 72.60 +/- 9.75 ng/ml) and fibroids of the uterus (mean +/- SD: 72.31 +/- 9.19 ng/ml) (p < 0.0001). CONCLUSIONS: In women with ovarian and uterine cancer a significant increase in the concentration of calprotectin was observed, suggesting an inflammatory process that accompanies cancer. These changes are especially pronounced in women with cancer which probably indicates autocrine production of the protein by cancer cells. Estimation of the parameter examined in the serum may improve differential diagnosis of malignant and benign ovarian and uterine cancers, however it requires further investigation.


Assuntos
Biomarcadores Tumorais/sangue , Complexo Antígeno L1 Leucocitário/sangue , Neoplasias Ovarianas/sangue , Neoplasias Uterinas/sangue , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Humanos , Inflamação/sangue , Pessoa de Meia-Idade , Polônia , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
Detalhe da pesquisa