RESUMO
We aimed to evaluate the impact of nutrient supplementation of beef female calves at pre-weaning on adipogenic determination. Thirty-four female calves were assigned to two experimental treatments: Control (CON, n = 17), where animals were supplemented only with mineral mixture; Supplemented (SUP, n = 17), where animals received energy-protein supplement containing minerals (5 g/kg of BW per day) of their body weight. Animals were supplemented from 100 to 250 days of age, and muscle samples were biopsied at the end of the supplementation period. Regarding the performance variables, there were no differences between treatments for initial body weight (P = 0.75). The final body weight (P = 0.07), average daily gain (P = 0.07), rib eye area (P = 0.03), and rib fat thickness (P = 0.08) were greater in SUP female calves compared with CON treatment. The number of fibro-adipogenic progenitor cells (P = 0.69) did not differ between treatments, while a greater number of intramuscular pre-adipocytes were observed in SUP than CON female calves (P = 0.01). The expression of miRNA-4429 (P = 0.20) did not differ between treatments, while the expression of miRNA-129-5p (P = 0.09) and miRNA-129-2-3p (P = 0.05) was greater in CON than SUP female calves. Our results suggest that nutrient supplementation at early postnatal stages of development enhances the commitment of fibro-adipogenic progenitor cells into the adipogenic lineages allowing to an increase in intramuscular fat deposition potential of the animals later in life.
Assuntos
Dieta , MicroRNAs , Bovinos , Animais , Feminino , Dieta/veterinária , Desmame , Ração Animal/análise , Suplementos Nutricionais , Peso Corporal , NutrientesRESUMO
Thirty non-castrated male lambs with 20±2.3 kg average body weight (BW) were randomly assigned to five treatments consisted of different dietary concentrations of crude glycerin (CG; 0, 3, 6, 9 and 12% on DM basis) to evaluate the effects on performance, carcass and meat quality traits. A quadratic effect was observed for performance (P=0.04), final BW (P<0.01) and hot carcass weight (P<0.01). No effects of CG were observed (P>0.05) on carcass pH neither on shear-force, cooking loss and ether extract content in longissimus. The inclusion of CG tended to reduce the Zn content in meat (P=0.09). The data suggests that CG (36.2% of glycerol and 46.5% of crude fat) may be used in diets of finishing lambs with concentrations up to 3% without negative effects on performance and main carcass traits. Moreover, inclusion of CG seems to not affect quality and safety of meat for human consumption.
Assuntos
Gorduras na Dieta/administração & dosagem , Glicerol/administração & dosagem , Carne/análise , Matadouros , Ração Animal/análise , Animais , Culinária , Dieta/veterinária , Proteínas Alimentares/análise , Masculino , Metais Pesados/análise , Músculo Esquelético/química , Carneiro Doméstico , Zinco/análiseRESUMO
This study evaluated the effect of feed restriction and goat genotype on meat quality. Three genotypes (Brazilian native breed Canindé; Brazilian native breed Moxotó; and F1 Boer crossbred animals obtained by crossing Boer bucks with local breed does) and three different feeding regimens (ad libitum fed, AL; restricted fed at 75% of the ad libitum, R.75; or restricted fed at 50% of the average ad libitum intake, R.50) were used. There was no difference (P>0.05) in chemical composition, total and soluble collagen, and shear force of the Longissimus lumborum muscle among genotypes. However, AL had greater amounts of soluble collagen and crude protein in the muscle (P<0.05) than R.75. No difference (P>0.05) was observed for the myofibrillar fragmentation index. The goat genotype presented few differences in their fatty acid profiles. However, goats fed ad libitum had a more favorable fatty acid profile for human health with greater concentrations of oleic acid, unsaturated fatty acids, and conjugated linoleic acid.
Assuntos
Colágeno/análise , Gorduras na Dieta/análise , Ingestão de Energia , Ácidos Graxos Insaturados/análise , Carne/análise , Proteínas Musculares/análise , Músculo Esquelético/química , Ração Animal , Animais , Brasil , Cruzamento , Dieta , Proteínas Alimentares/análise , Cabras/genética , Humanos , Carne/normas , Miofibrilas , Estresse MecânicoRESUMO
Twenty-four pregnant Nellore cows were randomly assigned into 2 feeding level groups (control [CTL]; fed 1.0 times the maintenance requirement; n = 12; and overnourished [ON]; fed at 1.5 times the maintenance requirement; n = 12) to evaluate effects of maternal overnutrition on fetal skeletal muscle development. Cows were slaughtered at 135, 190, and 240 d of gestation and samples of fetal LM were collected for analysis of mRNA expression analysis and for histological evaluation of collagen content and number of muscle cells. There was no interaction between gestational period and maternal nutrition for the variables evaluated (P > 0.05). The mRNA expression of Cadherin-associated protein, ß 1 (ß-catenin) tended to be greater in fetuses from ON cows (P = 0.08), while myogenic differentiation 1 (MyoD; P = 0.56), myogenin (MyoG; P = 0.70), and the number of muscle cells (P = 0.90) were not affected by maternal overnutrition. Gestational period did not affect the mRNA expression of ß-catenin (P = 0.60) and MyoG (P = 0.21). The mRNA expression of MyoD tended to increase with days of gestation (P = 0.06). The mRNA expression of zinc finger protein 423 (Zfp423; P < 0.0001), C/EBPα (P = 0.01), and PPARγ (P < 0.0001) were enhanced in ON fetuses. No effects of days of gestation were observed for mRNA expression of Zfp423 (P = 0.75) and C/EBPα (P = 0.48). The mRNA expression of PPARγ in fetuses at 190 d of gestation tended to be greater than those at 135 and 240 d of gestation (P = 0.06). The mRNA expression of transforming growth factor ß (TGF-ß; P < 0.0001), collagen type III, α I (COL3A1; P < 0.0001), and collagen content (P = 0.01) were increased in ON fetuses. Gestational period did not affect the mRNA expression of collagen type I, α I (COL1A1; P = 0.65). The mRNA expression of COL3A1 (P = 0.09) in fetuses at 190 d of gestation tended to be greater than fetuses at 135 and 240 d of gestation. The mRNA expression of TGF-ß in fetuses at 190 d of gestation was greater than in fetuses at 135 d of gestation (P = 0.03), and the values observed in fetuses at 240 d of gestation did not differ from the other gestational time points. The least value of collagen content (P = 0.01) was observed in fetuses at 135 d of gestation, and no differences were observed among the other gestational time points. These data shows that maternal overnutrition enhances fibrogenesis and likely adipogenesis without compromising myogenesis in fetal skeletal muscle of cattle.
Assuntos
Bovinos , Colágeno/metabolismo , Feto/metabolismo , Músculo Esquelético/metabolismo , Hipernutrição , RNA Mensageiro/metabolismo , Adipogenia , Animais , Biomarcadores , Proteína alfa Estimuladora de Ligação a CCAAT/metabolismo , Feminino , Regulação da Expressão Gênica , Fenômenos Fisiológicos da Nutrição Materna , PPAR gama/metabolismo , Gravidez , RNA Mensageiro/genética , Fatores de Transcrição/metabolismo , beta Catenina/metabolismoRESUMO
Intramuscular fat and collagen content are major factors affecting beef quality, but mechanisms regulating intramuscular adipose and connective tissue deposition are far from clear. Japanese Wagyu cattle are well known for their extremely high marbling. The objective of this study was to evaluate intramuscular fat (IMF) and collagen deposition in the muscle of Wagyu compared with Angus cattle. Animals were managed under the same condition and slaughtered at an averaging 585 ± 12.1 kg of BW. Samples of sternomandibularis muscle were collected from Wagyu (n = 3) and Angus (n = 3) for molecular and histological investigations of adipogenesis and fibrogenesis. With exception of C/EBPß (P = 0.2864), the expression of the adipogenic markers C/EBPα (P = 0.008), PPARγ (P = 0.028), and zip finger protein 423 (Zfp423; P = 0.047) in Wagyu were greater than in Angus muscle, which was consistent with greater IMF deposition in Wagyu (P < 0.05). In addition, more adipocytes and preadipocytes were detected intramuscularly in Wagyu cattle. Similarly, fibrogenesis was also enhanced in Wagyu, with a greater expression of fibroblast growth factor (FGF)-2 (P = 0.028), FGF receptor 1 (P = 0.030), transforming growth factor (TGF)-ß (P = 0.028), collagen I (P = 0.012), and collagen III (P = 0.025). Similarly, Wagyu muscle had greater collagen content (P = 0.002) and decreased collagen solubility (P = 0.005). In addition, muscle fiber diameter was larger (P < 0.0001) in Wagyu than in Angus cattle. These results clearly show that both IMF and collagen contents are enhanced in Wagyu cattle and more adipogenic cells are detected in Wagyu muscle, indicating intramuscular adipogenesis is enhanced in Wagyu compared with Angus muscle.
Assuntos
Adipogenia , Bovinos/genética , Bovinos/metabolismo , Fibroblastos/metabolismo , Músculo Esquelético/metabolismo , Tecido Adiposo/metabolismo , Animais , Proteína alfa Estimuladora de Ligação a CCAAT/genética , Proteína alfa Estimuladora de Ligação a CCAAT/metabolismo , Colágeno/metabolismo , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Fibroblastos/citologia , Marcadores Genéticos , Masculino , Microscopia de Fluorescência/veterinária , PPAR gama/genética , PPAR gama/metabolismo , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Dedos de ZincoRESUMO
Carcass and meat quality traits of 16 pregnant and 5 non-pregnant cows fed at 1.2 times maintenance and 16 pregnant and 6 non-pregnant fed ad libitum were evaluated. Pregnancy did not affect final body weight (FBW; P=0.0923), cold carcass yield (CCY; P=0.0513), longissimus muscle area (LMA; P=0.8260), rib fat thickness (RFT; P=0.1873) and shear force (WBSF; P=0.9707). A lower FBW (P=0.0028), LMA (P=0.0048) and RFT (P=0.0001) were observed in feed restricted cows. However, no differences were found for CCY (P=0.7243) and WBSF (P=0.0759) among feeding level groups. These data suggests that carcass and meat quality traits are not affected by pregnancy status in Nellore cows. Moreover, although cows experiencing feed restriction did have reduced deposition of subcutaneous fat and lean tissue, there were no major impacts on meat quality traits.
Assuntos
Tecido Adiposo/metabolismo , Composição Corporal , Peso Corporal , Ingestão de Energia , Carne/análise , Músculo Esquelético/metabolismo , Gravidez , Ração Animal , Animais , Compartimentos de Líquidos Corporais/metabolismo , Restrição Calórica , Bovinos , Dieta , Gorduras na Dieta/análise , Dronabinol/análogos & derivados , Feminino , Carne/normas , Músculo Esquelético/anatomia & histologia , Estresse Mecânico , Gordura Subcutânea/metabolismoRESUMO
The dynamics of human and animal adipogenesis has been defined using several traditional cell systems including stromal vascular cells and adipocyte-related cell lines. But a relatively new cell system using progeny cells stemming from the dedifferentiation of purified cultures of mature adipocytes may be used for studying the development and biology of adipocytes. In this research, we show that isolated (and purified) mature adipocytes derived from Wagyu cattle dedifferentiate into progeny cells, and that these spindle-shaped, proliferative-competent daughter cells possess ability to proliferate. We outline the optimum cell culture system and offer precautionary thoughts for effective mature adipocyte culture. Collectively, this represents a novel cell model which may provide new insights into cell development, physiology and use as a model for animal production/composition, tissue engineering and disease treatment.
Assuntos
Adipócitos/citologia , Diferenciação Celular , Animais , Bovinos , Desdiferenciação Celular , Proliferação de Células , Separação Celular , Células Cultivadas , HumanosRESUMO
Carcass and meat quality traits of thirty-six feedlot beef heifers from different genetic groups (GG) fed at two concentrate levels (CL) were evaluated using 12 - Nellore (NE), 12 - ½Angus x ½Nellore (AN) and 12 - ½Simmental x ½Nellore (SN) animals. Six heifers of each GG were randomly assigned into one of two treatments: concentrate at 0.8% or 1.2% of body weight (BW). Heifers fed concentrate at 0.8% of BW had greater (P<0.05) dressing percentage. None of the proximate analysis components of the beef were affected (P>0.05) by either CL or GG. Heifers from the AN group had higher (P<0.05) carcass weights, 12th rib fat thickness and lower dressing percentage (P<0.05) compared to the other groups. NE heifers had greater WBSF values (P<0.05) than the other genetic groups. Data suggest that the concentrate level can be reduced without compromising meat quality traits.
Assuntos
Bovinos/genética , Dieta/veterinária , Carne , Tecido Adiposo , Ração Animal , Animais , Composição Corporal , Peso Corporal , Feminino , Hibridização Genética , Músculo Esquelético/química , Miofibrilas/químicaRESUMO
Carcasses of sixty-three Nellore bulls slaughtered at a commercial beef plant were randomly selected by dental classification (2, 4, 6 or 8 permanent incisors) in order to evaluate the influence of dental maturity on carcass traits and meat quality. Carcasses with 8 permanent incisors (p.i.) had greatest values (P<0.05) of carcass weight and longissimus area. Carcasses with 4 and 6 p.i. presented similar values of rib fat thickness being greater (P<0.05) than the other groups. Carcasses with 6 and 8 p.i. presented greater (P<0.05) values of shear force than the other groups. Conversely, carcasses with 2 and 4 p.i. displayed greater (P<0.05) myofibrillar fragmentation index and collagen solubility. Greatest values of thawing loss were observed in carcasses with 2 p.i. (P<0.05) while carcasses with 8 p.i. presented greatest values (P<0.05) of drip loss. Regarding longissimus color, carcasses with 8 p.i presented greatest value (P<0.05) of b*. Data suggests that dental maturity influences carcass traits and meat quality of Nellore bulls.
Assuntos
Composição Corporal , Incisivo/crescimento & desenvolvimento , Gordura Intra-Abdominal/crescimento & desenvolvimento , Carne/análise , Músculo Esquelético/crescimento & desenvolvimento , Adiposidade , Animais , Peso Corporal , Brasil , Bovinos , Colágeno/análise , Colágeno/química , Dentição Permanente , Concentração de Íons de Hidrogênio , Masculino , Carne/normas , Indústria de Embalagem de Carne/métodos , Proteínas Musculares/análise , Músculo Esquelético/química , Pigmentação , Controle de Qualidade , Resistência ao Cisalhamento , Solubilidade , Temperatura , Água/análiseRESUMO
Avaliou-se o efeito dos níveis de suplementação sobre o desempenho, as características de carcaça, a composição corporal e as exigências de energia e proteína de bovinos de corte sob pastejo. A área experimental constituiu-se de cinco piquetes formados com Brachiaria decumbens. Utilizaram-se 22 bovinos não castrados, com peso corporal médio inicial de 320kg e idade de 18 meses. Três animais foram abatidos ao início do experimento para servirem como referência. Dos 19 animais restantes, três foram designados ao grupo mantença (MT), os outros 16 foram distribuídos em quatro tratamentos: controle ou mistura mineral (MM), 1kg, 2kg e 3kg de suplemento ao dia. Observou-se aumento linear no consumo de matéria seca (CMS), energia digestível (CED) e energia metabolizável (CEM); o consumo de nutrientes digestíveis totais (CNDT) foi máximo para o consumo de 2,88kg de suplemento. O ganho médio diário (GMD) máximo, 0,91kg/dia, foi observado para o consumo de suplemento de 2,69kg/dia. Verificou-se aumento linear no peso corporal em jejum (PCJ), no peso de corpo vazio (PCVZ) e no peso da carcaça para os animais suplementados. Não foi observado efeito da suplementação sobre o rendimento da carcaça. Verificou-se aumento sobre a área de olho de lombo (AOL) e a espessura de gordura subcutânea (EGS), quando os animais não suplementados foram comparados com os suplementados. Não foi observado efeito para o comprimento da carcaça (CCA), nem para os rendimentos dos cortes comerciais. As exigências líquidas de energia e proteína apresentaram, respectivamente, aumento e redução com o aumento do PC dos animais. As exigências de energia líquida para mantença (ELm) foram de 85 kcal/PCVZ0,75. As exigências de energia metabolizável para mantença foram estimadas em 130,08 kcal/PCVZ0,75. A suplementação de bovinos no período de transição águas-seca aumenta o consumo de MS e energia, promovendo aumento do ganho de peso dos animais.
This experiment aimed to evaluate the performance, carcass characteristics, body composition, and energy and protein requirements of beef cattle under grazing conditions. The experimental area constituted of 5 paddocks with Brachiaria decumbens as forage source. Twenty two steers (Zebu) with mean body weight and ages of 320kg and 18 months, respectively, were used. Three animals were slaughtered at the beginning of the experiment and used as standard. Of the nineteen remaining animals, three were designated for maintenance group and the others were randomly submitted to one of four treatments: mineral mixture (MM), 1, 2 or 3kg of supplement daily. There was a linear increase in dry matter intake (DMI), digestible energy (DEI) and metabolizable energy (MEI), and total digestible nutrients intake (TDNI) was maximum consumption of 2.88kg of supplement. The maximum average daily gain (ADG), 0.91kg/day, was observed for supplement intake of 2.69kg/day. Linear increase was observed in shrunk body weight (SBW), EBW and carcass weight. There was no effect on the carcass allowance, however, it was observed on the ribeye area and back fat thickness. No effect was observed on carcass length and commercial cuts. The requirement of net energy (NE) and net protein (NP) increased and decreased as the animals BW increased. The maintenance requirement for net (NEm) and metabolizable energy were 85 kcal/EBW0.75 and 130 kcal/EBW0.75. Supplementation of cattle in the period of transition between rainy and dry seasons increased the dry matter and energy intake, promoting an increase in animal weight gain.
RESUMO
Avaliou-se o efeito da substituição do milho por glicerina bruta em dietas para suínos em terminação. Foram utilizados 80 suínos, machos castrados, híbrido comercial, com média de peso de 67kg, em um experimento em delineamento inteiramente ao acaso, com cinco tratamentos e oito repetições, com dois animais por unidade experimental. Os tratamentos foram: 0,0; 4,0; 8,0; 12,0 e 16,0% de glicerina bruta em substituição ao milho nas dietas. A substituição do milho por glicerina bruta não afetou as características de desempenho (P>0,05). Observaram-se efeito linear crescente de tratamento (P<0,05) sobre a espessura de toucinho na carcaça e efeito linear decrescente de tratamento (P<0,05) sobre a perda de líquido no descongelamento e na força de cisalhamento. A glicerina bruta pode substituir em até 16,0% do milho da dieta para suínos em terminação, sem prejuízos do desempenho e rendimento de carcaça, com melhoras na qualidade da carne.
We evaluated the effect of replacing corn with crude glycerin in diets for finishing pigs. We used 80 pigs, steers, commercial hybrids, with an average weight of 67kg, in an experiment with a completely randomized design with five treatments and eight replicates of two animals per experimental unit. The treatments were 0.0, 4.0, 8.0, 12.0, and 16.0% crude glycerin as a replacement for maize in diets. The substitution of corn with crude glycerin did not affect performance characteristics (P>0.05). We observed an increasing linear effect of the treatment (P<0.05) on backfat thickness and decreasing linear effect of the treatment (P<0.05) on the loss of fluid in the thawing and shearing force. The crude glycerin can replace up to 16.0% of the corn diet for finishing pigs without loss of performance and carcass yield, with improvements in meat quality.
Assuntos
Animais , Dieta/veterinária , Glicerol/análise , Glicerol/efeitos adversos , Suínos/crescimento & desenvolvimento , Ração Animal , Suínos/metabolismoRESUMO
The effects of urea levels on feed intake, digestibility and carcass traits of 27 confined cross bred steers weighing 303.25 ± 60.8kg, were evaluated. After weighing, castrating, vaccinating and a period of seven days, three steers were slaughtered at the beginning of the experiment and the remaining 24 were allotted in a completely randomized design to four isonitogenous experimental diets (12 percent of crude protein) and increasing level of urea on the dry matter-basis (0.0; 0.65; 1.30 and 1.65 percent) to replace soybean meal, so that the final content of the experimental diets were approximately 22, 37, 50 and 63 percent of crude protein in the form of NPN. Corn and elephant grass silages were supplied at a 70:30 ratio and the total diet had a 65:35, forage: concentrate ratio on a DM-basis. The urea level did not affect nutrient intake, except for rumen degradable protein (RDP) and total digestible nutrients (TDN) linearly increased in function of diet urea level. The nutrient digestibilities linearly increased except for ether extract and non fiber carbohydrate (NFC). A quadratic effect of diet urea level on daily weight gain and no effect of urea level on carcass traits were observed. Urea can totally replace soybean meal in diets for confined crossed dairy steers allowing gains of 1kg/day. Urea can enhance nutrient digestibility of diets.
Foram avaliados os efeitos dos níveis de uréia sobre o consumo, digestibilidade e características da carcaça de 27 novilhos (303,25 ± 60,80) confinados. Três animais foram abatidos no início do experimento e os 24 restantes foram distribuídos em delineamento inteiramente ao acaso em quatro tratamentos: 0; 0,65; 1,30 e 1,95 por cento de uréia na MS total da dieta, em substituição ao farelo de soja. A dieta final continha em torno de 22, 37, 50 e 63 por cento da PB na forma de compostos nitrogenados não-protéicos. Como volumoso foi utilizada uma mistura de 65 por cento de silagem de milho e silagem de capim-elefante na proporção 70:30, respectivamente. O consumo dos nutrientes não foi afetado pelos tratamentos, com exceção para o consumo de PDR e NDT que aumentaram linearmente. As digestibilidades dos nutrientes apresentaram comportamento linear crescente com exceção das digestibilidades do EE e CNF. Com relação ao desempenho dos animais, houve efeito quadrático dos tratamentos sobre o GMD. Não foi verificado nenhum efeito sobre as características de carcaça avaliadas. A uréia pode substituir completamente o farelo de soja na dieta de novilhos mestiços leiteiros em confinamento, permitindo ganhos de peso próximos a 1kg/dia. O uso de uréia na dieta pode melhorar a digestibilidade dos nutrientes.