RESUMO
The survival of the rho(+) factor and of Drug(R) mitochondrial genetic markers after exposure to ethidium bromide has been studied. A technique allowing the determination of Drug(R) genetic markers among a great number of both grande and petite colonies has been developed. The results have been analyzed by the target theory. The survival of the rho(+) factor is always less than the survival of any Drug(R) genetic marker. The survivals of C(R) and E(R) are similar to each other, while that of O(R) is greater than that of the other two Drug(R) markers. All possible combinations of Drug(R) markers have been found among the rho(-) petite cells induced, while the only type found among the grande colonies is the preexisting one. The loss of the C(R) and E(R) genetic markers was found to be the most frequently concomitant, while the correlation between the loss of the O(R) marker and the other two Drug(R) markers is less strong. Similar results have been obtained after U.V. irradiation. Interpretations concerning the structure of the yeast mitochondrial genome are given and hypotheses on the mechanism of petite mutation discussed.
Assuntos
Resistência Microbiana a Medicamentos , Mitocôndrias , Mutação , Saccharomyces cerevisiae , Etídio/farmacologia , Genótipo , Mutagênicos , Efeitos da Radiação , Recombinação Genética , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/efeitos da radiação , Raios UltravioletaRESUMO
We have isolated 15 spontaneous mutants resistant to one or several antibiotics like chloramphenicol, erythromycin and spiramycin. We have shown by several criteria that all of them result from mutations localized in the mitochondrial DNA. The mutations have been mapped by allelism tests and by two- and three-factor crosses involving various configurations of resistant and sensitive alleles associated in cis or in trans with the mitochondrial locus omega which governs the polarity of genetic recombination. A general mapping procedure based on results of heterosexual (omega(+)x omega(-)) crosses and applicable to mutations localized in the polar segment is described and shown to be more resolving than that based on results of homosexual crosses. Mutations fall into three loci which are all linked and map in the following order: omega-R(I)-R(II)-R(III). The first locus is very tightly linked with omega while the second is less linked to the first. Mutations of similar resistance phenotype can belong to different loci and different phenotypes to the same locus. Mutations confer antibiotic resistance on isolated mitochondrial ribosomes and delineate a ribosomal segment of the mitochondrial DNA. Homo- and hetero-sexual crosses between mutants of the ribosomal segment and those belonging to the genetically unlinked ATPase locus, O(I), have been performed in various allele configurations. The polarity of recombination between R(I), R(II), R(III) and O(I) decreases as a function of the distance of the R locus from the omega locus rather than as a function of the distance of the R locus from the O(I) locus.
Assuntos
Cloranfenicol/farmacologia , DNA Mitocondrial , Eritromicina/farmacologia , Leucomicinas/farmacologia , Mutação , Saccharomyces cerevisiae/efeitos dos fármacos , Adenosina Trifosfatases/metabolismo , Sequência de Bases , Mapeamento Cromossômico , Cruzamentos Genéticos , DNA Bacteriano , Resistência Microbiana a Medicamentos , Teste de Complementação Genética , Genética Microbiana , Fenótipo , RNA Ribossômico , Recombinação Genética , RibossomosRESUMO
We have characterized the abdominal-A locus in a Hymenopteran, the ant Myrmica rubra. The sequence of the homeotic domain of the Abdominal-A family of orthologous genes is known for a fairly large number of insects but the complete sequence of the Abdominal-A protein, is known only for a few. The two proteins of Drosophila melanogaster and Tribolium castaneum differ markedly outside the homeodomain. A comparison of the ant Abdominal-A protein sequences with those of these two insects shows that the ant and beetle sequences are very similar all along the length of the protein. The fruit fly has diverged considerably and equally from the other two insects. This divergence reflects different rates of evolution of the protein in different lineages.
Assuntos
Formigas/genética , Evolução Molecular , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Homeodomínio/genética , Sequência de Aminoácidos , Animais , Formigas/classificação , Formigas/crescimento & desenvolvimento , Proteínas de Insetos/genética , Dados de Sequência Molecular , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
Cyp1p (Hap1p) activates, among others, the two structural genes, CYC1 and CYP3 (CYC7) which encode isocytochromes c in Saccharomyces cerevisiae. This activation is believed to occur through the binding of the protein to the dissimilar upstream activation sequences (UASs), UAS1 and UAS', present upstream of CYC1 and CYP3, respectively. In this paper, we describe a novel promoter mutation, CYP3-5, which results from a 39-bp deletion located about 160 bp upstream of the well-characterized CYP3 UAS. This deletion includes a sequence identical to the 3' moiety of the CYC1 UAS1. Strikingly, a sequence identical to the 5' part of the CYC1 UAS1 is also present 60 bp downstream of the 3' half in the wild-type gene, suggesting that a spatial organization of the promoter might lead to the reconstitution in vivo of an active UAS1-like sequence. Interestingly, we find that in the presence of the CYP3-5 mutation, which disrupts this potential UAS1, the CYP-UAS' complex is importantly diminished and the transcription of CYP3 is insensitive to the wild-type CYP1-activating protein.