Temperature as an effective biosecurity tool against invasive snakes.

The application of thermal treatments could serve as a biosecurity tool to avert snake introduction, which often leads to irreversible ecological impacts. Here, we tested the applicability of conductive heating and thermal fumigation to prevent the spread of the California kingsnake (Lampropeltis californiae), a damaging species established on the island of Gran Canaria, likely to reach other vulnerable regions, and included among the most concerning invasive species of the European Union. We exposed 24 individuals to a thermal gradient to determine species selected temperatures, range of preferred temperatures and voluntary thermal maximum and used other 24 individuals to analyse their response to conductive heating, thermal fumigation and control (no heat) treatments. Lampropeltis californiae selected temperatures of 27.80 ± 1.05 °C (ranging 26.86 °C ± 1.21 °C to 28.68 °C ± 1.25 °C) and a voluntary thermal maximum of 32.50 ± 3.69 °C. Conductive heating and thermal fumigation performed equally well, inducing the exit of 83.33% and 91.67% of all individuals after 14.36 ± 9.25 min and 11.13 ± 8.60 min of exposition, and at a body temperature of 35.66 ± 3.53 °C and 35.57 ± 2.41 °C, respectively. Control treatments produced the exit of 29.17% of all individuals, which came out of the box in 24.80 ± 8.83 min and at a body temperature of 28.60 ± 1.38 °C. Thermal treatments could serve as an effective tool to prevent the inadvertent transportation of L. californiae and other invasive snakes threatening numerous regions around the globe.

Invasive snake causes massive reduction of all endemic herpetofauna on Gran Canaria.

Invasive snakes represent a serious threat to island biodiversity, being responsible for far-reaching impacts that are noticeably understudied, particularly regarding native reptiles. We analysed the impact of the invasive California kingsnake, Lampropeltis californiae-recently introduced in the Canary Islands-on the abundance of all endemic herpetofauna of the island of Gran Canaria. We quantified the density in invaded and uninvaded sites for the Gran Canaria giant lizard, Gallotia stehlini, the Gran Canaria skink, Chalcides sexlineatus, and Boettger's wall gecko, Tarentola boettgeri. We used spatially explicit capture-recapture and distance-sampling methods for G. stehlini and active searches under rocks for the abundance of the other two reptiles. The abundance of all species was lower in invaded sites, with a reduction in the number of individuals greater than 90% for G. stehlini, greater than 80% for C. sexlineatus and greater than 50% for T. boettgeri in invaded sites. Our results illustrate the severe impact of L. californiae on the endemic herpetofauna of Gran Canaria and highlight the need for strengthened measures to manage this invasion. We also provide further evidence of the negative consequences of invasive snakes on island reptiles and emphasize the need for further research on this matter on islands worldwide.

Could climate change benefit invasive snakes? Modelling the potential distribution of the California Kingsnake in the Canary Islands.

The interaction between climate change and biological invasions is a global conservation challenge with major consequences for invasive species management. However, our understanding of this interaction has substantial knowledge gaps; this is particularly relevant for invasive snakes on islands because they can be a serious threat to island ecosystems. Here we evaluated the potential influence of climate change on the distribution of invasive snakes on islands, using the invasion of the California kingsnake (Lampropeltis californiae) in Gran Canaria. We analysed the potential distribution of L. californiae under current and future climatic conditions in the Canary Islands, with the underlying hypothesis that the archipelago might be suitable for the species under these climate scenarios. Our results indicate that the Canary Islands are currently highly suitable for the invasive snake, with increased suitability under the climate change scenarios tested here. This study supports the idea that invasive reptiles represent a substantial threat to near-tropical regions, and builds on previous studies suggesting that the menace of invasive reptiles may persist or even be exacerbated by climate change. We suggest future research should continue to fill the knowledge gap regarding invasive reptiles, in particular snakes, to clarify their potential future impacts on global biodiversity.

Spatial ecology to strengthen invasive snake management on islands.

Knowledge on the spatial ecology of invasive predators positively contributes to optimizing their management, especially when involving cryptic and secretive species, such as snakes. However, this information is lacking for most invasive snakes, particularly on islands, where they are known to cause severe ecological and socio-economic impacts. This research is focused on assessing the spatial ecology of the California kingsnake (Lampropeltis californiae) on Gran Canaria to strengthen management actions. We monitored 15 radio-tagged individuals once per day on 9-11 days per month from July 2020 to June 2021 to calculate the species' home range and describe annual activity patterns in the invaded range. To account for the species' diel activity during the emergence period, we additionally monitored snakes from January to May 2021 during three consecutive days per month in four different time intervals each day. We detected movement (consecutive detections at least 6 m apart) in 31.68% of the 1146 detections during the whole monitoring period. Movements most frequently detected were shorter than 100 m (82.24%), and among them the range 0-20 m was the most recurrent (27.03%). The mean distance of movement was 62.57 ± 62.62 m in 1-2 days. Average home range was 4.27 ± 5.35 ha-calculated with the Autocorrelated Kernel Density Estimator (AKDE) at 95%-and did not significantly vary with SVL nor sex. We detected an extremely low value of motion variance (0.76 ± 2.62 σ2m) compared to other studies, with a general inactivity period from November to February, January being the less active month of the year. Diel activity was higher during central and evening hours than during early morning and night. Our results should be useful to improve control programs for this invasive snake (e.g., trap placement and visual survey guidance) on Gran Canaria. Our research highlights the importance of gathering spatial information on invasive snakes to enhance control actions, which can contribute to the management of secretive invasive snakes worldwide.

Coupling phenotypic changes to extinction and survival in an endemic prey community threatened by an invasive snake.

When facing novel invasive predators, native prey can either go extinct or survive through exaptation or phenotypic shifts (either plastic or adaptive). Native prey can also reflect stress-mediated responses against invasive predators, affecting their body condition. Although multiple native prey are likely to present both types of responses against a single invader, community-level studies are infrequent. The invasive California kingsnake (Lampropeltis californiae) a good example to explore invasive predators' effects on morphology and body condition at a community level, as this invader is known to locally extinct the Gran Canaria giant lizard (Gallotia stehlini) and to notably reduce the numbers of the Gran Canaria skink (Chalcides sexlineatus) and the Boettger's gecko (Tarentola boettgeri). By comparing a set of morphological traits and body condition (i.e. body index and ectoparasite load) between invaded and uninvaded areas for the three squamates, we found clear evidence of a link between a lack of phenotypic change and extinction, as G. stehlini was the single native prey that did not show morphological shifts. On the other side, surviving C. sexlineatus and T. boettgeri exhibited phenotypic differences in several morphological traits that could reflect plastic responses that contribute to their capacity to cope with the snake. Body condition responses varied among species, indicating the potential existence of simultaneous consumptive and non-consumptive effects at a community level. Our study further highlights the importance addressing the impact of invasive predators from a community perspective in order to gain a deeper understanding of their effect in native ecosystems.