[Lower jawbone structures symmetry evaluation using cone beam computed tomography]. / Otsenka simmetrichnosti kostnykh struktur nizhnei chelyusti po dannym konusno-luchevoi komp'yuternoi tomografii.

OBJECTIVE: To assess the prevalence and quantitative characteristics of skeletal asymmetries of the body and lower jaw branches in three-dimensional space. MATERIALS AND METHODS: Using depersonalized data archive, of 400 CBCT scans, forty studies were picked randomly. Patients aged 25 to 35 years participated in research, no history of maxillofacial reconstructive surgery; gender, race, and facial anomaly were ignored. Three experienced doctors, independently, identified 15 reference points on radiographic images of AUTOPLAN software. Expert consensus found in the orientation determination. Six planes and eight points were constructed, using Math algorithms and the Python OOP; body and branches of the lower jaw length measurements performed and processed automatically. RESULTS: The study identified symmetry, using a method for determining parameters of the lower jaw in 3D space (Priority Reference No.2023100466 at 10.01.2023), modern dentistry research method. Both sides of data obtained shown asymmetry of the body and branches of the lower jaw occurred in 45% (18 people) and 67.5% (27 people) of cases. Forty percent of cases were noted with Mild asymmetry of the lower jaw body (16 people), moderate asymmetry noted in 5% (2 people). These parameters were 57.5% for branches, (23 people) and 10% (4 people). Combined form of anomaly observed in 37.5% (15 people) of cases, among which 12 people (75%) showed adaptive compensation of anomalies, which requires further investigation on a larger number of patients. CONCLUSION: Lower body jaw asymmetry is prevalent (80%). Patients with moderate and serious anomaly form appeared in 15% of cases.

[Oral hygiene and microcirculation of the mucous membrane of the tongue and gums dynamics in patients with burning mouth syndrome as a result of the use of a toothbrush with a gold-zinc electroplating]. / Dinamika gigieny rta i mikrotsirkulyatsii slizistoi obolochki yazyka i desny u bol'nykh s sindromom «pylayushchego rta¼ v rezul'tate primeneniya zubnoi shchetki s gal'vanoparoi zoloto-tsink.

THE AIM OF THE STUDY: Was to assess the level of oral hygiene and microcirculation of the mucous membrane of the tongue and gums in patients with burning mouth syndrome (BMS) as a result of using a toothbrush with a gold-zinc electroplating. MATERIALS AND METHOS: The study included 50 patients of both sexes aged 45-64 years with the diagnosis of BMS divided into two groups. In the main group, the complex of hygienic measures included the use of a toothbrush with a gold-zinc electroplating and a paste with the fluorine content of 1350 ppm. In the comparison group, a regular manual toothbrush and a paste with a fluoride content of 1350 ppm were used for oral hygiene. A control group consisted of 25 healthy people with the mean age of 44±4.5 years without clinical signs of BMS in the oral cavity and concomitant acute somatic pathologies. RESULTS: Tongue mucosa microcirculation level in patients using the toothbrush with a gold-zinc electroplating was significantly higher than in the comparison group using conventional manual toothbrush. Oral hygiene level assessment showed statistically significant better brushing efficacy in patients using the toothbrush with a gold-zinc electroplating. CONCLUSION: The use of a toothbrush with a gold-zinc electroplating contributes to the normalization of microcirculation in the mucous membrane of the tongue and gums and is recommended for inclusion in the complex of therapeutic measures in patients with BMS.

[Consistency of expert opinions on localization of the reference points for studying a soft tissue face profile in digital teleradiological images of the skull lateral projection]. / Soglasovannost' mnenii ekspertov pri izuchenii pozitsii opornykh tochek dlya izucheniya myagkotkannogo profilya litsa na tsifrovykh telerentgenologicheskikh snimkakh bokovoi proektsii cherepa.

OBJECTIVE: The aim of the study is to identify the consistency of expert opinions when manually identifying the reference points positions for studying the soft-tissue face profile in digital teleradiological images of the skull lateral projection. MATERIAL AND METHODS: The study involved 11 orthodontists having a 1 to 8 year experience. They localized the reference points manually, using the software with image enhancement options. Each doctor processed 100 X-ray images of the skull lateral projection. Totally, 1100 positions were identified (11 parameters in 100 images). The average position of 11 manual localizations was taken as the baseline. Then, manually identified positions were automatically compared with the basic localization. RESULTS: We have a good consistency of expert opinions when determining the reference points of the soft-tissue face profile. Herewith, the highest consistency of expert opinions is observed for soft-tissue reference points of the upper and lower lips (LL and EN points) with the average variation coefficients equal to 0.557726 and 0.566349 respectively; and the lowest consistency is observed for the hard tissue point Po with the average variation coefficient of 0.819904. Despite rather serious shortcomings in determining separate points (such as Or, DT, Po) in teleradiological images of the lateral skull projection, the method for determining soft-tissue profilometric reference points gives satisfactory results for more than 85.87% cases. In general, the method can be considered clinically reliable. However, the means and methods for identifying reference points require improving.

[Experimental study of the bearing capacity of lyophilized allogeneic bone material]. / Eksperimental'noe issledovanie nesushchei sposobnosti liofilizirovannogo allogennogo kostnogo materiala.

THE AIM OF THE STUDY: Was to conduct an experimental study of the bearing capacity and determination of the Young's modulus of lyophilized allogeneic bone material. MATERIALS AND METHODS: The study used 5 prototypes of lyophilized allogeneic bone blocks, which were alternately subjected to static loading by compressive force. The experimental values of Young's modulus of all bone samples were determined. When applying the Student's probability distribution in statistical processing of experimental data, the average indicators of the forces of destruction of the samples were obtained and Young's modulus was determined. RESULTS AND DISCUSSION: As a result of the study, the indices of the maximum loading (6012H) and Young's modulus (147.2±0.228 MPa) of lyophilized allogeneic bone material were obtained. CONCLUSION: The obtained Young's modulus of lyophilized allogeneic bone material is of great importance in scientific activity, providing the possibility of further investigation of the properties of lyophilized allogeneic bone tissue to form a preoperative forecast of the behavior of an osteoplastic material and a dental implant installed in it.

[Cephalometric analysis of lateral skull X-ray images using soft computing components in the search for key points]. / Tsefalometricheskii analiz rentgenologicheskikh snimkov bokovoi proektsii cherepa s pomoshch'yu komponentov myagkikh vychislenii v poiske klyuchevykh tochek.

THE AIM OF THE STUDY: Was to investigate the efficiency of decoding teleradiological studies using an algorithm based on the use of convolutional neural networks - a simple convolutional architecture, as well as an extended U-Net architecture. MATERIALS AND METHODS: For the experiment, a dataset was prepared by three orthodontists with over 10 years of clinical experience. Each of the orthodontists processed 100 X-ray images of the lateral projection of the head according to 27 parameters, 2700 measurements were made. The coordinates of the control points found by orthodontists in the images were compared with each other and a conclusion was made about the consistency of experts in the data obtained. RESULTS: The results of convolutional neural network CNN were not satisfactory in 17 (62.96%) features, satisfactory in 10 (37.04%). The assessment of orthodontists resulted in non-satisfactory evaluation in 6 (22.22%), satisfactory in 8 (29.63%), good in 8 (29.63%), and excellent in 5 (18.52%) coordinates. Neural networks with U-Net architecture showed satisfactory results in 9 (33.3%) cases, good in 16 (59.3%) and excellent in 2 (7.4%) cases, with no non-satisfactory results. CONCLUSION: The neural network of the U-Net architecture is more effective than a simple fully convolutional neural network and its results of determining anatomical reference points on two-dimensional images of the head are relatively comparable with the data obtained by medical specialists.

[Technology of manufacturing of personalized reconstructive allogenic bone graft (in Russian only)]. / Tekhnologiia proizvodstva personifitsirovannogo rekonstruktivnogo allogennogo kostnogo implantata.

AIM: To develop the technology of manufacturing of reconstructive allogenic bone graft with individual parameters by using of digital prototyping and modeling. MATERIAL AND METHODS: Allogeneic cadaveric bone blocks were used as material. The following methods were used to develop a manufacturing technology for personalized allogenic reconstructive bone implant: harvesting and special preparation of cadaveric material, primary disinfection, viral inactivation, ultrasound management, lyophilization of bone material, CT-examination of patients with various bone defects, digital prototyping and modeling, milling on a machine with numerical program control, X-ray sterilization. RESULTS: The technology of manufacturing of reconstructive allogenic bone graft with individual parameters was successfully tested in the treatment of 24 patients with post-traumatic, destructive-dystrophic and degenerative defects of jaws in 2012-2015. Final reconstructive allogenic bone implant has a personalized 3D-geometry in accordance with individual maxillofacial anatomy of particular patient. One of implant surfaces is fully congruent to the jaw bone defect, the others form the width, height and volume of reconstructive bone implant considering personified maxillofacial features. All above-mentioned features of bone implant are important to restore chewing function of dental system. CONCLUSION: The problem of bone implant personification may be resolved by using of computed tomography followed by digital analysis of jaw structure, technologies for digital reconstruction of bone defects (digital prototyping and modeling) and digital manufacturing.

[Estimated error of bone digital solid 3D-model construction algorithm]. / Otsenka pogreshnosti algoritma postroeniia tsifrovoi tverdotel'noi 3D-modeli kostnogo fragmenta.

The aim of the study was an experimental estimation of precision of maxilla bone digital solid 3D-model construction algorithm. The experiment was carried out on 12 mutton mandibles used 3D-model construction based on CT data. Estimated error was calculated for each of the stages of proposed processing algorithm including 3 software: ScanIP (triangle mesh calculation, primary solid 3D-model), ArtecStudio 9 (secondary procession for artefacts elimination), ZBrush 4R6 (triangle mesh simplification, final 3D-model). The proposed algorithm provides satisfactory precision for solid 3D-model with individual maxillary bone features.

[REVIEW OF EPIDEMIOLOGIC SITUATION ON HEMORRHAGIC FEVER WITH RENAL SYNDROME (HERS) IN RUSSIAN FEDERATION IN 1990 - 2015].

AIM: Analyze HFRS morbidity in Russian Federation during the last 25 years (1990 - 2015). MATERIALS AND METHODS: Official statistics of Federal Service for Surveillance on Consumers' Rights Protection and Human Weillbeing (CPS), including Federal Centre of Hygiene and Epidemiology, were used for the analysis, as well as materials from regional departments of CPS and centers of hygiene and epidemiology. Epidemiologic analysis was the main method. Statistical treatment of the results obtained was carried out using gener- ally accepted methods of variation statistics with elements of system analysis. Results; For the studied period (1990 - 2015) 194 116 cases of HFRS were registered. Morbidity was registered in 8 federal districts of the Russian Federation in 58 subjects. The most intense epidemiologic situation was noted in Privolzhsky Federal District, that accounted for 86.4% of total HFRS morbidity during the -studied period. Analysis of morbidity was carried out in every federal district, most epidemically unfavorable territories are shown. CONCLUSION: The data presented on HFRS morbidity reflect -unfavorable situation for this disease in Russian Federation. Measures to prevent the emergence of diseases to reduce the general level of morbidity in Russian Federation are presented.

[Preclinical tests of additive materials for personified endoprostheses of hand joints]. / Doklinicheskie ispytaniia additivnykh materialov dlia izgotovleniia personifitsirovannykh éndoprotezov sustavov kisti v éksperimente.

AIM: To evaluate the biocompatibility of additive materials for personified endoprostheses of hand joints in vivo. MATERIAL AND METHODS: We tested a material based on titanium that was implanted into muscles and bone tissue in experiment on rabbits. Follow-up was 30 and 90 days. RESULTS: Implantation into muscle tissue is accompanied by reaction against foreign body followed by fibrosis without concomitant inflammation. Induction of osteogenesis and trabecular structures remodeling were detected after implantation into bone tissue. CONCLUSION: Biocompatibility of tested titanium-based material was confirmed.

[EPIDEMIOLOGICAL DIFFERENTIATION OF NATURAL FOCI OF HEMORRHAGIC FEVER WITH RENAL SYNDROME OF THE REPUBLIC OF BASHKORTOSTAN].

AIM: Typization of natural foci of HFRS of the Republic of Bashkortostan and differentiation of their territories. by the degree of potential epidemic hazard. MATERIALS AND METHODS: Materials of epizootologic and epidemiologic monitoring of natural foci of HFRS of the Republic of Bashkortostan for 1980 - 2014 were used. Evaluation of area of territory with high, medium and low potential epidemic hazard by HFRS was carried out taking into account multi-year param- eters of morbidity by administrative districts of the Republic of Bashkortostan. 3 gradations of morbidity level by HFRS - more than 201, 101 - 200, less than 100 per 100 thousand of the population - were used for epidemiologic differentiation ofthe territory. RESULTS: 3 main landscape- epidemiologic types of HFRS foci were isolated - forest, forest-barrens, barrens, as well as their landscape variants. The area with a high degree of potential epidemic hazard by HFRS was estab= lished to be around 3.8%; medium - 15.9%, low - 80.3%.of the whole territory of the Republic of Bashkortostan. CONCLUSION: -A necessity of a differentiated approach to territories with various degree of potential epidemic hazard by HFRS is justified during execution of prophylaxis activi- ties.

[IMPACT OF CASPIAN SEA LEVEL FLUCTUATIONS ON THE EPIZOOTIC ACTIVITY OF THE CASPIAN SANDY NATURAL PLAGUE FOCUS].

There is evidence that in 1923-2014 the sharp aggravations of the epizootic situation of plague in the area of its Caspian sandy natural focus after long interepizootic periods are in time with the ups of the Caspian Sea in the extrema of 11-year solar cycles. There were cases of multiple manifestations of plague in the same areas in the epizootic cycles of 1946-1954, 1979-1996, 2001, and 2013-2014. The paper considers the possible role of amebae of the genus Acanthamoeba and nematodes, the representatives of the orders Rhabditida and Tylenchida in the microfocal pattern of plague manifestations.

[Prevalence and genetic diversity of the Alongshan virus (Flaviviridae) circulating in ticks in the south of Eastern Siberia].

INTRODUCTION: Tick-borne infections are of great importance for many regions of Russia, including Eastern Siberia. This unfavorable epidemiological situation can be characterized not only by the circulation of well-known tick-borne infections, but also by the identification of new pathogens, the role of which remains little or generally unexplored. Multicomponent flavi-like viruses can cause infectious diseases in humans and pose a threat to public health. The purpose of the study was the identification and molecular genetic characterization of the Alongshan virus (Flaviviridae, ALSV) isolates, transmitted by ticks in the south of Eastern Siberia. MATERIALS AND METHODS: Total 1060 ticks were collected and analyzed from the territory of the Republics of Khakassia, Tuva, Buryatia, Irkutsk Region and Transbaikal Territory (Zabaykalsky Krai) in the spring-summer period 2023. ALSV RNA was detected by RT-PCR followed by nucleotide sequence determination and phylogenetic analysis for each segment of the genome. RESULTS: The ALSV infection rate in Ixodes persulcatus ticks collected in the Republic of Khakassia was 3.3% (95% CI: 1.4-7.5); in Irkutsk Oblast - 1.0% (95% CI: 0.3-3.7); in the Republic of Tuva - 0.9% (95% CI: 0.3-3.4) and in Transbaikal Krai - 0.7% (95% CI: 0.2-3.6). Sequences of all four segments of ALSV genetic variants circulating in I. persulcatus ticks in the south of Eastern Siberia are grouped with sequences found in China and clustered into the Asian subgroup transmitted by taiga ticks. The level of difference in the nucleotide sequences of genome fragments among the identified genetic variants of ALSV ranged from 2 to 3%. CONCLUSION: The article shows the widespread distribution of ALSV in I. persulcatus ticks in the Republics of Khakassia and Tyva, Irkutsk Oblast and Transbaikal Territory. The obtained data actualize monitoring of changes in the area of distribution of potentially dangerous for humans flavi-like viruses and their vectors.

[Improvement of typification of natural foci of plague based on ecological-genetic analysis of Yersinia pestis].

Contemporary features of distribution of various subspecies and biovars of plague causative agent by landscape-geographical zones and mountain belts on the territory of Russia and other CIS countries are examined. The most widely spread in plain and mountain natural foci were noted to be Yersinia pestis main subspecies medieval biovar strains. Strains of Y. pestis non-main subspecies are spread in mountain landscapes of Altai, Caucasus, Tian Shan. Change of dominating species of rodents considered as the main carriers of plague was noted not to result in change of genetic and biochemical characteristics of Y. pestis strains. Perspectives of study of "micro-focality" of plague are emphasized for deciphering the mechanism of the enzootic.

[Formation of focus of West Nile fever on the territory of Saratov Region].

AIM: Detection of circulation of West Nile virus (WNV) on the territory of Saratov Region and prerequisites for formation of natural focus of West Nile fever (WNF), determination of the role of WNV in infectious pathology on the territory of the region. MATERIALS AND METHODS: of organs of small mammals, birds, blood-sucking arthropods for the presence of WNV markers (antigens and/or RNA) were studied. Clinical material from patients with symptoms not excluding WNF was studied. Donor blood sera samples were analyzed with the aim of detection of immune layer against WNV in population of Saratov Region. RESULTS: In 2010 WNV antigens were detected by EIA in 12 samples (7.1%) of mammal organ suspensions. In 2012 by using RT-PCR and EIA, markers of WNV were detected in 6 samples of bird brain suspensions (6.3%) and 1 sample of mammal organ suspension. Immune layer of population against WNV was 4% in 2011, 2.8% in 2012. In 2012 in 11 of 27 examined patients IgM against WNV in diagnostic titers and/or serconversion of IgG in paired sera were detected. In addition in 5 individuals virus RNA was detected in blood. Based on clinical, laboratory data and epidemiologic anamnesis 11 patients were diagnosed with WNF. CONCLUSION: The results obtained give evidence on the circulation of WNV on the territory of Saratov Region in 2010 - 2012. With the development of complications of WNF epidemiologic situation in 2012 an expansion of WNV areal onto the territory of the region took place and the process of formation of WNF natural foci is ongoing.

[Reasons of low epizootic activity of natural foci of plague in Russia at the beginning of the 21st century].

AIM: Establish the main reasons of low epizootic activity of natural foci of plague in Russian Federation in 2000-2009. MATERIALS AND METHODS: Multiyear data on the number of isolated Yersinia pestis strains, area of detected epizootic sites, amounts of the field material studied for plague were used to evaluate annual activity of natural foci. RESULTS: In the current decade mountain and high-mountain natural foci of plague were characterized by constant high epizootic activity. In plains and low-mountain natural foci a low periodic epizootic activity was noticed, a development of intra-epizootic periods. A conditioning role of helioclimatic factors on the status of parasitic systems and epizootic activity of natural foci of plague was noticed. CONCLUSION: The warming of climate in 1990-2007 was the main reason for low epizootic activity of plains natural foci of plague in Russia. In the contemporary conditions of a recurrent cooling (from 2008) and an increase of humidity in the Northern, North-Western pre-Caspian and pre-Caucasus territories, a new significant activation of them is predicted there with a peak in 2017-2019.

[Molecular mechanisms of the plague pathogenic agent interaction with invertebrates].

Microbe Russian Anti-Plague Research Institute, Saratov, Russia The literature data and experimental results of the authors on the molecular basis of plague agent interaction with invertebrates are discussed. The details of the plague agent life cycle, its genome organization, and molecular genetic mechanisms of its survival in flea vector and on the nematode cuticule are discussed. The experimental data about the ability to form biofilms at abiotic and biotic surfaces in the Yersinia pestis strains of the main and non-main subspecies are presented. Mechanisms of horizontal and vertical transmission of plague agent are considered. The suggestion about participation of the new member in the complex parasitic biocenosis (nematode, vector parasite) is put forward.

[Phenomenon of Yersinia pestis biofilm formation in flea organism].

For the first time substantiated was the role of the phenomenon of Yersinia pestis biofilm--extracellular matrix envelope (EME)--formation as the basis that determines the nature of plague agent interaction with flea organism. Implication of vector's proventriculus in the process of biofilm formation was demonstrated. Ultrastucture of plague microbe conglomerates in flea proventriculus and midgut was analysed and uniform mechanism of their formation was elucidated. The role of Yersinia pestis biofilm in plague agent preservation in the gut of ectoparasites and soil of rodents burrows was discussed. PCR analysis confirmed the presence of plague microbe in plague infected corpses and fleas excrements stored at +8-10 degreesC for 7 years 9 months.

[The manifestation of necrophagy in the populations of rodents carrying the virus of hemorrhagic fever with renal syndrome].

The role of necrophagy in the epizootic manifestations of hemorrhagic fever with renal syndrome (HFRS) is first shown. By analyzing a great body of data obtained in the Saratov Region in 1982-2000, it has been established that the frequency of manifestations of necrophagy depends on many factors, the most important of which are a season, the size (density) of populations of small mammals, their species composition and the type of biotopes inhabited by these animals. Necrophagy is ascertained to be of great importance in HFRS foci as one of the alimentary routes of infection transmission in the parasitic systems. The presence or absence of necrophagy may serve as a preliminary test for the activity of HFRS foci.