RESUMO
BACKGROUND: It is unclear whether mucosal inflammation has an effect on the bone under the mucosa in patients with chronic rhinosinusitis (CRS). OBJECTIVES: The aim of this study was evaluation of inflammatory cytokines genes expression in bone tissue taken from the patients who had undergone endoscopic sinus surgery for CRS. METHODS: A total group of a consecutive 49 patients with diagnosis of chronic rhinosinusitis based on EPOS 2007 criteria undergoing endoscopic sinus surgery for CRS were enrolled in the study. Based on histopathologic findings of the mucosal and bone tissues we evaluated the rate of inflammation. Expression of target genes: interleukin 1ß (IL1ß), interleukin 6 (IL6), interleukin 11 (IL11), tumor growth factor ß (TGF ß) and tumor necrosis factor α (TNF α) were analysed by real-time PCR method in samples of the ethmoid bone taken during endoscopic sinus surgery for CRS. RESULTS: Based on histopathological findings in the studied population we found symptoms of osteitis in 5 patients. In the studied population we found significant differences between patients with osteitis and without osteitis with respect to IL6 gene expression in bone tissue (p=0.0003), IL11 gene expression (p=0.02) and TNFα gene expression in bone tissue (p=0.0035). CONCLUSION: In our study we have demonstrated that in some patients with CRS and coexisting symptoms of osteitis some inflammatory markers genes expression are increased in this population.
Assuntos
Osso Etmoide , Interleucina-11/genética , Interleucina-6/genética , Osteíte , Rinite , Sinusite , Fator de Necrose Tumoral alfa/genética , Adolescente , Adulto , Idoso , Doença Crônica , Endoscopia , Osso Etmoide/metabolismo , Osso Etmoide/patologia , Feminino , Expressão Gênica , Marcadores Genéticos , Humanos , Masculino , Pessoa de Meia-Idade , Mucosa Nasal/metabolismo , Mucosa Nasal/patologia , Osteíte/etiologia , Osteíte/genética , Osteíte/patologia , Rinite/complicações , Rinite/metabolismo , Rinite/patologia , Rinite/fisiopatologia , Rinite/cirurgia , Sinusite/complicações , Sinusite/metabolismo , Sinusite/patologia , Sinusite/fisiopatologia , Sinusite/cirurgiaRESUMO
Our original technique of preparing urinary and seminal sediment was modified in order to identify neoplastic cells in cases of testicular malignancies. In a series of 24 patients with testicular tumor-like conditions, 18 tumors were diagnosed, 12 cytologically. In one case, the result of cytological examination was falsely positive since histopathology revealed necrosis of the testicle only. The morphology of neoplastic cells in the seminal sediment is demonstrated.
Assuntos
Sêmen/citologia , Neoplasias Testiculares/patologia , Citodiagnóstico , Humanos , Masculino , Necrose , Valor Preditivo dos Testes , Neoplasias Testiculares/urinaRESUMO
We present clinical and radiological findings in a case of collecting (Bellini) duct carcinoma (CDC). This is a rare and aggressive kidney cancer originating from the distal renal tubule. The patient underwent radical nephrectomy and the pathological report showed trabeculopapillary, partially solid adenocarcinoma infiltrating the renal capsule and sinus. Immunohistochemical, as well as mucinocarminic and PAS staining studies are necessary for diagnosis of CDC. Surprisingly, one year follow-up studies were negative for recurrent disease. Chromosomal findings are usually different from most often diagnosed clear cell renal cell cancers. Findings from the literature are briefly discussed.
Assuntos
Adenocarcinoma/patologia , Neoplasias Renais/patologia , Túbulos Renais Coletores , Adenocarcinoma/diagnóstico por imagem , Adenocarcinoma/cirurgia , Idoso , Humanos , Imuno-Histoquímica , Neoplasias Renais/diagnóstico por imagem , Neoplasias Renais/cirurgia , Masculino , RadiografiaRESUMO
Leydig cell tumours deriving from the gonadal stroma represent one per cent of all testicular tumours. They may occur at any age. Ten per cent of cases are malignant. There is no evidence that they are prone to develop in undescended testis. We report the rare case of leydigioma in 71 year old man with unilateral cryptorchidism. Only a few cases have been reported as arising from undescended testis.
Assuntos
Criptorquidismo/complicações , Tumor de Células de Leydig/etiologia , Neoplasias Testiculares/etiologia , Idoso , Humanos , Tumor de Células de Leydig/patologia , Masculino , Neoplasias Testiculares/patologiaAssuntos
Derivação Arteriovenosa Cirúrgica/efeitos adversos , Ilhotas Pancreáticas/metabolismo , Transplante de Pâncreas/efeitos adversos , Transplante de Pâncreas/métodos , Animais , Velocidade do Fluxo Sanguíneo , Cães , Glucagon/metabolismo , Rejeição de Enxerto , Insulina/metabolismo , Secreção de Insulina , Transplante de Pâncreas/fisiologia , Artéria Esplênica/cirurgia , Veia Esplênica/cirurgia , Trombose/prevenção & controleRESUMO
The purpose of present study was to evaluate the sensitivity of regenerating epithelium of renal proximal convolutions to mercury bichloride and to describe light microscopic, electron microscopic and histochemical abnormalities occurring under the effect of the latter. The ligation of rat kidney with silk thread resulted in ischemic necrosis of tubules of compressed area followed by vivid regeneration. In that period mercury bichloride was administered intraperitoneally. The first detectable lesions appeared in mitochondrial swelling, condensation of endoplasmic reticulum, a decreased number of ribosomes and the presence of numerous vocuoles. Later on, the regenerating epithelium lost its brush broder and single cells became necrotic. During 42 days of observation the sequelae of mercuric intoxication were only focal and particular cells showed injury of variable degree.
Assuntos
Túbulos Renais/efeitos dos fármacos , Mercúrio/farmacologia , Regeneração/efeitos dos fármacos , Fosfatase Alcalina/metabolismo , Animais , Epitélio/enzimologia , Epitélio/ultraestrutura , Túbulos Renais/patologia , Túbulos Renais/fisiologia , Masculino , Ratos , Succinato Desidrogenase/metabolismoRESUMO
This report deals with a very uncommon benign testicular tumour, fibrolipoma. Orchidectomy was followed by the removal of the lymph nodes from the region of external iliac vessels. No recurrence appeared during 6-year follow-up.
Assuntos
Lipoma/cirurgia , Neoplasias Testiculares/cirurgia , Idoso , Humanos , Linfonodos/cirurgia , Masculino , Orquiectomia , Indução de RemissãoRESUMO
The results of irradiation of the internal wall of bladder in rabbits with YAG:Ne laser developed at the Institute of Optoelectronics, Military Technical Academy in Warsaw are presented. The value of this laser in coagulation of the wall of bladder at 35W and exposure 0.2-8.0 seconds is stressed out. Results justify clinical trials with the use of this type of laser in the treatment of the urinary neoplasms in men.
Assuntos
Fotocoagulação a Laser , Bexiga Urinária/cirurgia , Animais , Fotocoagulação a Laser/métodos , Coelhos , Bexiga Urinária/patologiaRESUMO
Two cases of osteogenic sarcoma localized in the femoral neck have been presented. As it has resulted from literature, such a localization is very rare and it matches great diagnostic difficulties, mainly caused by absence of typical periarticular reactions and ambiguous X-ray pictures. Angiography and computerized tomography are helpful in diagnosis of the type of tumor. Histopathological examination is decisive.
Assuntos
Neoplasias Femorais/diagnóstico , Osteossarcoma/diagnóstico , Adolescente , Adulto , Angiografia , Feminino , Colo do Fêmur , Humanos , Masculino , Tomografia Computadorizada por Raios XRESUMO
An attempt to repair ureter with the neodium YAG laser in rabbits has been reported. Laser designed by the Institute of Opto-electronics at the Military Technical Academy in Warsaw has been used to anastomose previously cut off ureter. The result has been favourable in 60% of cases. Stenosis making the urine outflow from the kidney difficult might be contributed to technical problems.
Assuntos
Terapia a Laser , Ureter/cirurgia , Anastomose Cirúrgica/métodos , Animais , CoelhosRESUMO
The results of irradiation of the urinary bladder wall with cw YAG:Nd laser beam are presented. The use of irradiation times ranging from 02, and 0.8 second destroyed irradiated tissues up to a half of the muscular laser.
Assuntos
Terapia a Laser/métodos , Bexiga Urinária/cirurgia , Humanos , Bexiga Urinária/patologiaRESUMO
The results of experimental ureteral anastomoses with laser Nd:YAG are discussed. Cut ureters were anastomosed in all animals. Anastomosis consisted of the scar of the connective tissue covered with intermediate epithelium and scarring process was quicker, if modelling catheter was used.
Assuntos
Fotocoagulação/métodos , Modelos Biológicos , Ureter/cirurgia , Alumínio , Anastomose Cirúrgica/métodos , Animais , Cateteres de Demora , Cães , Minerais , Neodímio , Radiografia , Ureter/citologia , Ureter/diagnóstico por imagem , Cateterismo Urinário/instrumentação , Cicatrização , ÍtrioRESUMO
The authors discuss effects of YAG:Nd laser beam of 35W and exposure time between 0.4 and 0.8 second on the internal surface of bladder wall in two dogs. Such an energy destroyed bladder tissues to not more than a half of their thickness.
Assuntos
Terapia a Laser/métodos , Bexiga Urinária/cirurgia , Animais , Cães , Fatores de Tempo , Bexiga Urinária/patologiaRESUMO
The technique for detection of neoplastic cells in the seminal fluid of patients with testicular malignancies is presented. This preliminary study of 7 patients with testicular tumors and 50 controls demonstrates the possibility of using cytological examination of the sperm as the screening method for testicular tumors.
Assuntos
Disgerminoma/patologia , Sêmen/citologia , Neoplasias Testiculares/patologia , Adulto , Disgerminoma/diagnóstico , Humanos , Masculino , Neoplasias Embrionárias de Células Germinativas/patologia , Neoplasias Testiculares/diagnósticoRESUMO
Tumour-like changes in the masseter may be diagnosed as haemangiomas, lymphomas, lipomas, hernia of the muscle, benign hypertrophy or inflammatory or traumatic lesions. The authors describe a case of haemorrhagic cyst of rare location in the masseter and analysed the clinical features of this lesion.
Assuntos
Cistos/diagnóstico , Músculo Masseter , Adolescente , Diagnóstico Diferencial , Feminino , Hemorragia , HumanosRESUMO
OBJECTIVE: To introduce computer-based analysis of Feulgen-stained urinary bladder cell nuclei from voided urine to identify neoplastic urothelial nuclei. STUDY DESIGN: Nuclei from 23 healthy people and 33 patients with urinary bladder cancer were analyzed. The nuclei from 9 cancer patients with grade G1 (stage Ta), 17 with grade G2 (stages Ta, T1, T1a and T2) and 7 with grade G3 (stages Cis, Ta + Tis, T1 and T3b) were analyzed. Image analysis was carried out by means of a digital image processing system designed by the authors. Features describing nuclei were selected as the first step of the procedure. Then a multistage classifier was constructed to identify positive and negative cases. RESULTS: The results of this pilot study of a group of 56 patients yielded a 71% correct classification rate in the control group, while a 66% rate was obtained among the cancer patients. The sensitivity of the method was 100% and the specificity was 77%. CONCLUSION: This approach to the identification of neoplastic urothelial nuclei may be sufficiently well developed to be used successfully both in screening high-risk populations and in clinical practice.