The Use of Malic and Acetic Acids in Washing Solution to Control Salmonella spp. on Chicken Breast.

Salmonella is a persisting contaminant in poultry products that may pose a potential risk to consumers. Thus, developing decontamination strategies to eliminate or reduce this pathogen in chicken is crucial. The objective of the current study was to investigate the antimicrobial activity of malic acid (MA) and acetic acid (AA) or their combination against Salmonella on chicken breast at 4 °C for 10 days. The effect of storage temperature (4 and 21 °C) on Salmonella inactivation was also investigated for up to 21 days. Five serovars of Salmonella were inoculated in a model Mueller-Hinton (MH) broth system to a level of about 7 log10 CFU/mL and the broth was treated with 5 mg/mL of each of MA, AA or their combination. AA was more effective than MA in the model system at 21 °C, where it resulted in total elimination of Salmonella, but MA was more effective in eliminating Salmonella at 4 °C. However, the combined MA and AA solutions were more effective than either MA or AA alone. When applying washing solutions containing 5 mg/mL of either of MA, AA, or their combination to chicken breast inoculated with about 5 log10 CFU/g, the MA+AA washing solution was the most effective. It resulted in complete elimination of Salmonella from chicken breast and rendered a significant reduction in mesophilic aerobic bacteria and lactic acid bacteria numbers. PRACTICAL APPLICATION: This study indicates that the use of a washing solution containing MA and AA could improve the safety and extend the shelf life of raw chicken by substantially reducing Salmonella and contaminating microflora on the product.

Socio-demographic and dietary factors associated with obesity among female university students in Jordan.

OBJECTIVE: This study aimed to explore the socio-demographic and dietary factors that may be associated with obesity among female university students in Jordan. METHODS: A cross-sectional survey was carried out among 406 female students recruited from two universities in Jordan. Data were collected through self-reporting, using a previously validated questionnaire. Weight and height were measured and body mass index was calculated to determine the weight status of the participants. RESULTS: High educational level of mothers [odds ratio (OR)=1.25] and monthly pocket money of more than 200 Jordanian Dinars (OR=1.67) were found to be risk factors for obesity, whereas a sibling ranking of more than six was a protective factor (OR=0.31). Those who were eating from the university cafeteria had double the risk for obesity (OR=2.41) than those who did not. Regular eating of meals and snacking between meals were found to be protective factors (OR ranged from 0.42 to 0.79). Regular consumption of milk products, fruit, canned fruit juices, bakery products and legumes were found to be protective factors. In contrast, the regular consumption of potato chips (OR=1.35), chicken (OR=1.51), and fish (OR=1.45) were found to be risk factors for obesity. Using a chi-square test, none of the factors studied showed significant association with obesity. CONCLUSION: A program to promote healthy eating among university students in Jordan should consider the local socio-demographic and food behavior factors that could be related to obesity to ensure the effectiveness of such a program.