RESUMO
Strong ultraviolet (UV) radiation at high altitude imposes a serious selective pressure, which may induce skin pigmentation adaptation of indigenous populations. We conducted skin pigmentation phenotyping and genome-wide analysis of Tibetans in order to understand the underlying mechanism of adaptation to UV radiation. We observe that Tibetans have darker baseline skin color compared with lowland Han Chinese, as well as an improved tanning ability, suggesting a two-level adaptation to boost their melanin production. A genome-wide search for the responsible genes identifies GNPAT showing strong signals of positive selection in Tibetans. An enhancer mutation (rs75356281) located in GNPAT intron 2 is enriched in Tibetans (58%) but rare in other world populations (0 to 18%). The adaptive allele of rs75356281 is associated with darker skin in Tibetans and, under UVB treatment, it displays higher enhancer activities compared with the wild-type allele in in vitro luciferase assays. Transcriptome analyses of gene-edited cells clearly show that with UVB treatment, the adaptive variant of GNPAT promotes melanin synthesis, likely through the interactions of CAT and ACAA1 in peroxisomes with other pigmentation genes, and they act synergistically, leading to an improved tanning ability in Tibetans for UV protection.
Assuntos
Adaptação Fisiológica , Altitude , Pigmentação da Pele , Aciltransferases/genética , Adaptação Fisiológica/genética , Etnicidade , Humanos , Melaninas/genética , Fenótipo , Pigmentação da Pele/genética , Tibet , Transcriptoma , Raios UltravioletaRESUMO
BACKGROUND: Domestication and introduction of dairy animals facilitated the permanent human occupation of the Tibetan Plateau. Yet the history of dairy pastoralism in the Tibetan Plateau remains poorly understood. Little is known how Tibetans adapted to milk and dairy products. RESULTS: We integrated archeological evidence and genetic analysis to show the picture that the dairy ruminants, together with dogs, were introduced from West Eurasia into the Tibetan Plateau since ~ 3600 years ago. The genetic admixture between the exotic and indigenous dogs enriched the candidate lactase persistence (LP) allele 10974A > G of West Eurasian origin in Tibetan dogs. In vitro experiments demonstrate that - 13838G > A functions as a LP allele in Tibetans. Unlike multiple LP alleles presenting selective signatures in West Eurasians and South Asians, the de novo origin of Tibetan-specific LP allele - 13838G > A with low frequency (~ 6-7%) and absence of selection corresponds - 13910C > T in pastoralists across eastern Eurasia steppe. CONCLUSIONS: Results depict a novel scenario of genetic and cultural adaptations to diet and expand current understanding of the establishment of dairy pastoralism in the Tibetan Plateau.
Assuntos
Criação de Animais Domésticos , Povo Asiático , Dieta , Leite , Animais , Cães/genética , Humanos , Tibet , RuminantesRESUMO
Local wild bovids have been determined to be important prey on the northeastern Tibetan Plateau (NETP), where hunting game was a major subsistence strategy until the late Neolithic, when farming lifestyles dominated in the neighboring Loess Plateau. However, the species affiliation and population ecology of these prehistoric wild bovids in the prehistoric NETP remain unknown. Ancient DNA (aDNA) analysis is highly informative in decoding this puzzle. Here, we applied aDNA analysis to fragmented bovid and rhinoceros specimens dating â¼5,200 y B.P. from the Neolithic site of Shannashuzha located in the marginal area of the NETP. Utilizing both whole genomes and mitochondrial DNA, our results demonstrate that the range of the present-day tropical gaur (Bos gaurus) extended as far north as the margins of the NETP during the late Neolithic from â¼29°N to â¼34°N. Furthermore, comparative analysis with zooarchaeological and paleoclimatic evidence indicated that a high summer temperature in the late Neolithic might have facilitated the northward expansion of tropical animals (at least gaur and Sumatran-like rhinoceros) to the NETP. This enriched the diversity of wildlife, thus providing abundant hunting resources for humans and facilitating the exploration of the Tibetan Plateau as one of the last habitats for hunting game in East Asia.
Assuntos
Biodiversidade , Bovinos , DNA Antigo/análise , Genoma/genética , Migração Animal , Animais , Bovinos/classificação , Bovinos/genética , DNA Mitocondrial , História Antiga , Comportamento de Retorno ao Território Vital , Humanos , Perissodáctilos/classificação , Perissodáctilos/genética , Dinâmica Populacional/história , Ruminantes/classificação , Ruminantes/genética , TibetRESUMO
Due to their rapid development and wide application in modern agriculture, robots, mobile terminals, and intelligent devices have become vital technologies and fundamental research topics for the development of intelligent and precision agriculture. Accurate and efficient target detection technology is required for mobile inspection terminals, picking robots, and intelligent sorting equipment in tomato production and management in plant factories. However, due to the limitations of computer power, storage capacity, and the complexity of the plant factory (PF) environment, the precision of small-target detection for tomatoes in real-world applications is inadequate. Therefore, we propose an improved Small MobileNet YOLOv5 (SM-YOLOv5) detection algorithm and model based on YOLOv5 for target detection by tomato-picking robots in plant factories. Firstly, MobileNetV3-Large was used as the backbone network to make the model structure lightweight and improve its running performance. Secondly, a small-target detection layer was added to improve the accuracy of small-target detection for tomatoes. The constructed PF tomato dataset was used for training. Compared with the YOLOv5 baseline model, the mAP of the improved SM-YOLOv5 model was increased by 1.4%, reaching 98.8%. The model size was only 6.33 MB, which was 42.48% that of YOLOv5, and it required only 7.6 GFLOPs, which was half that required by YOLOv5. The experiment showed that the improved SM-YOLOv5 model had a precision of 97.8% and a recall rate of 96.7%. The model is lightweight and has excellent detection performance, and so it can meet the real-time detection requirements of tomato-picking robots in plant factories.
Assuntos
Solanum lycopersicum , Frutas , Agricultura , Algoritmos , Movimento CelularRESUMO
This study investigated the sources, contamination and ecological risks of polycyclic aromatic hydrocarbons (PAHs) based on their spatiotemporal distribution in aquatic environment in the Wuhan section of the Yangtze River (WYR). The fugacity ratio evaluation indicated that sediment was secondary release sources of two- and three-ring PAHs and sinks of four- and five-ring PAHs. The total concentrations of PAHs (Σ16PAHs) ranged from 2.51 to 102.5 ng/L in water with the dominant contribution of 47.8% by two-ring PAHs. Σ16PAHs in sediments varied from 5.90 to 2926 ng/g with the contribution of 35.4% by four-ring PAHs. The higher levels of PAHs occurred around developed industrial areas during the wet season, which was related to local industrial emissions and influenced by rainfall/runoff. Annual flux of Σ16PAHs was estimated of 28.77 t. The PMF model analysis revealed that petroleum and industrial emissions were the dominant sources in water accounting for 58.5% of the total pollution, although traffic emission was the main source for sediment accounting for 44.6%. Risk assessments showed that PAHs in water were at low risks, whereas about 44% of the sediments were identified as medium risks. Therefore, energy structure adjustment and further implement of regulation and monitoring are necessary to reduce PAH emissions.
Assuntos
Hidrocarbonetos Policíclicos Aromáticos , Poluentes Químicos da Água , Hidrocarbonetos Policíclicos Aromáticos/análise , Rios/química , Monitoramento Ambiental , Poluentes Químicos da Água/análise , Sedimentos Geológicos/química , ChinaRESUMO
The rise and expansion of Tibetan Empire in the 7th to 9th centuries AD affected the course of history across East Eurasia, but the genetic impact of Tibetans on surrounding populations remains undefined. We sequenced 60 genomes for four populations from Pakistan and Tajikistan to explore their demographic history. We showed that the genomes of Balti people from Baltistan comprised 22.6-26% Tibetan ancestry. We inferred a single admixture event and dated it to about 39-21 generations ago, a period that postdated the conquest of Baltistan by the ancient Tibetan Empire. The analyses of mitochondrial DNA, Y, and X chromosome data indicated that both ancient Tibetan males and females were involved in the male-biased dispersal. Given the fact that the Balti people adopted Tibetan language and culture in history, our study suggested the impact of Tibetan Empire on Baltistan involved dominant cultural and minor demic diffusion.
Assuntos
Fluxo Gênico , Genoma Humano , Feminino , Humanos , Masculino , Paquistão , Tibet/etnologia , Sequenciamento Completo do GenomaRESUMO
BACKGROUND: Yaks that inhabit the Tibetan Plateau exhibit striking phenotypic and physiological differences from cattle and have adapted well to the extreme conditions on the plateau. However, the mechanisms used by these animals for the regulation of gene expression at high altitude are not fully understood. RESULTS: Here, we sequenced nine lung transcriptomes of yaks at altitudes of 3400, 4200 and 5000 m, and low-altitude Zaosheng cattle, which is a closely related species, served as controls. The analysis identified 21,764 mRNAs, 1377 circRNAs and 1209 miRNAs. By comparing yaks and cattle, 4975 mRNAs, 252 circRNAs and 75 miRNAs were identified differentially expressed. By comparing yaks at different altitudes, we identified 756 mRNAs, 64 circRNAs and 83 miRNAs that were differentially expressed (fold change ≥2 and P-value < 0.05). The pathways enriched in the mRNAs, circRNAs and miRNAs identified from the comparison of yaks and cattle were mainly associated with metabolism, including 'glycosaminoglycan degradation', 'pentose and glucuronate interconversions' and 'flavone and flavonol biosynthesis', and the mRNAs, circRNAs and miRNAs identified from the comparison of yaks at different altitude gradients were significantly enriched in metabolic pathways and immune and genetic information processing pathways. The core RNAs were identified from the mRNA-miRNA-circRNA networks constructed using the predominant differentially expressed RNAs. The core genes specific to the difference between yaks and cattle were associated with the endoplasmic reticulum and fat deposition, but those identified from the comparison among yaks at different altitude gradients were associated with maintenance of the normal biological functions of cells. CONCLUSIONS: This study enhances our understanding of the molecular mechanisms involved in hypoxic adaptation in yaks and might contribute to improvements in the understanding and prevention of hypoxia-related diseases.
Assuntos
Altitude , Bovinos/genética , MicroRNAs , RNA Circular , RNA Mensageiro , Animais , MicroRNAs/genética , RNA Circular/genética , RNA Mensageiro/genética , TranscriptomaRESUMO
Iron/zinc (Fe/Zn), phosphoric acid (H3PO4) or in combination (Fe/Zn + H3PO4) modified sludge biochar (SBC) were prepared and tested in this study to adsorb fluoroquinolones antibiotics including ciprofloxacin (CIP), norfloxacin (NOR) and ofloxacin (OFL) from water. Fe/Zn + H3PO4-SBC had an increased surface area (SBET), total pore volume (Vtot), mesoporous volume (Vmes), pore diameter (Dp) and oxygen-containing functional groups. It exhibited superior adsorption performance for CIP, NOR and OFL with the maximum adsorption amount of 83.7, 39.3, 25.4 mg g-1, respectively. Pseudo-second kinetic and Freundlich isotherm model presented the better fitting. The results of models and characterization analysis in combination indicated that physisorption and chemisorption, including pore filling, hydrogen bonding, π-π interaction, electrostatic interaction and functional groups complexation on a heterogeneous surface were the dominant process and mechanism. Liquid film diffusion was the main rate-limiting step. The adsorption process of CIP, NOR and OFL onto Fe/Zn + H3PO4-SBC were a spontaneous endothermic process. This study demonstrated that Fe/Zn + H3PO4 modified SBC exhibited high adsorption capacity, which was a promising adsorbent for fluoroquinolones as well as for other antibiotics effective removal from waters.
Assuntos
Antibacterianos/análise , Carvão Vegetal/química , Fluoroquinolonas/análise , Esgotos/química , Poluentes Químicos da Água/análise , Purificação da Água/métodos , Adsorção , Ferro/química , Cinética , Modelos Teóricos , Ácidos Fosfóricos/química , Propriedades de Superfície , Zinco/químicaRESUMO
Human skin color diversity is considered an adaptation to environmental conditions such as UV radiation. Investigations into the genetic bases of such adaptation have identified a group of pigmentation genes contributing to skin color diversity in African and non-African populations. Here, we present a population analysis of the pigmentation gene KITLG with previously reported signal of Darwinian positive selection in both European and East Asian populations. We demonstrated that there had been recurrent selective events in the upstream and the downstream regions of KITLG in Eurasian populations. More importantly, besides the expected selection on the KITLG variants favoring light skin in coping with the weak UV radiation at high latitude, we observed a KITLG variant showing adaptation to winter temperature. In particular, compared with UV radiation, winter temperature showed a much stronger correlation with the prevalence of the presumably adaptive KITLG allele in Asian populations. This observation was further supported by the in vitro functional test at low temperature. Consequently, the pleiotropic effects of KITLG, that is, pigmentation and thermogenesis were both targeted by natural selection that acted on different KITLG sequence variants, contributing to the adaptation of Eurasians to both UV radiation and winter temperature at high latitude areas.
Assuntos
Aclimatação/genética , Povo Asiático/genética , Seleção Genética , Pigmentação da Pele/genética , Fator de Células-Tronco/genética , Temperatura Baixa , Feminino , Humanos , Masculino , Raios UltravioletaRESUMO
Field coupled samples in soil and grass were collected to determine the concentrations and identify the uptake pathways of PCBs into the grass at a pasture from Scotland, UK. Concentrations of indicator PCBs (∑7PCBs) in soils ranged from 0.20 to 0.88â¯ngâ¯g-1 dw (dry weight), with a mean of 0.33â¯ngâ¯g-1 dw, and in grass ranged from 0.20 to 2.14â¯ngâ¯g-1 dw, with a mean of 0.48â¯ngâ¯g-1 dw. The comprehensive factors of low concentrations and detection rate (PCB28: 18.8%; PCB52: 37.5%) of PCBs in soil, as well as continuously declined air concentrations of PCBs in the UK since the 1990s suggested that the secondary emission from the soil is becoming the supplied source of PCBs to air and grass. The significant correlations between bioconcentration factor (BCF) values and the log KOW (Râ¯=â¯-0.850, pâ¯<â¯0.05) and log KOA (Râ¯=â¯-0.860, pâ¯<â¯0.05) of indicator PCB congeners were found in the present study, indicating that these two parameters are likely to affect the bioaccumulation and uptake of grass. A generic one-compartment model was employed to identify uptake pathways of grass and evaluate the uptake amounts for PCBs. This suggested that the most important pathway for uptake of PCBs by grass was at the aerial part, and the difference of PCBs concentrations between leaves and roots was about four orders of magnitude. Removing and risk transfer of PCBs or other organic pollutants by grass need to be investigated and assessed further.
Assuntos
Monitoramento Ambiental , Poaceae/química , Bifenilos Policlorados/análise , Poluentes do Solo/análise , Solo/química , Poluentes Ambientais/análiseRESUMO
Tibetans are well adapted to the hypoxic environments at high altitude, yet the molecular mechanism of this adaptation remains elusive. We reported comprehensive genetic and functional analyses of EPAS1, a gene encoding hypoxia inducible factor 2α (HIF-2α) with the strongest signal of selection in previous genome-wide scans of Tibetans. We showed that the Tibetan-enriched EPAS1 variants down-regulate expression in human umbilical endothelial cells and placentas. Heterozygous EPAS1 knockout mice display blunted physiological responses to chronic hypoxia, mirroring the situation in Tibetans. Furthermore, we found that the Tibetan version of EPAS1 is not only associated with the relatively low hemoglobin level as a polycythemia protectant, but also is associated with a low pulmonary vasoconstriction response in Tibetans. We propose that the down-regulation of EPAS1 contributes to the molecular basis of Tibetans' adaption to high-altitude hypoxia.
Assuntos
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Hipóxia/genética , Aclimatação/genética , Adaptação Biológica/genética , Adaptação Fisiológica/genética , Adulto , Altitude , Doença da Altitude/genética , Doença da Altitude/metabolismo , Animais , Regulação para Baixo , Etnicidade/genética , Feminino , Variação Genética/genética , Hemoglobinas , Humanos , Hipóxia/metabolismo , Prolina Dioxigenases do Fator Induzível por Hipóxia/genética , Desequilíbrio de Ligação/genética , Masculino , Camundongos , Camundongos Knockout , Polimorfismo de Nucleotídeo Único , Seleção Genética , TibetRESUMO
Skin lightening among Eurasians is thought to have been a convergence occurring independently in Europe and East Asia as an adaptation to high latitude environments. Among Europeans, several genes responsible for such lightening have been found, but the information available for East Asians is much more limited. Here, a genome-wide comparison between dark-skinned Africans and Austro-Asiatic speaking aborigines and light-skinned northern Han Chinese identified the pigmentation gene OCA2, showing unusually deep allelic divergence between these groups. An amino acid substitution (His615Arg) of OCA2 prevalent in most East Asian populations-but absent in Africans and Europeans-was significantly associated with skin lightening among northern Han Chinese. Further transgenic and targeted gene modification analyses of zebrafish and mouse both exhibited the phenotypic effect of the OCA2 variant manifesting decreased melanin production. These results indicate that OCA2 plays an important role in the convergent skin lightening of East Asians during recent human evolution.
Assuntos
Povo Asiático/genética , Proteínas de Membrana Transportadoras/genética , Pigmentação da Pele/genética , Adolescente , Alelos , Substituição de Aminoácidos , Evolução Biológica , População Negra/genética , Criança , Etnicidade/genética , Evolução Molecular , Feminino , Frequência do Gene , Estudos de Associação Genética/métodos , Variação Genética , Genética Populacional/métodos , Haplótipos , Humanos , Masculino , Proteínas de Membrana Transportadoras/sangue , Proteínas de Membrana Transportadoras/metabolismo , Polimorfismo de Nucleotídeo Único , Seleção Genética , Pigmentação da Pele/fisiologia , População Branca/genética , Adulto JovemRESUMO
Tibetans are well adapted to high-altitude environments. Among the adaptive traits in Tibetans, the relatively low hemoglobin level is considered a blunted erythropoietic response to hypoxic challenge. Previously, EPAS1 and EGLN1, the major upstream regulators in the hypoxic pathway, were reportedly involved in the hemoglobin regulation in Tibetans. In this study, we report a downstream gene (HMOX2) involved in heme catabolism, which harbors potentially adaptive variants in Tibetans. We first resequenced the entire genomic region (45.6 kb) of HMOX2 in Tibetans, which confirmed the previously suspected signal of positive selection on HMOX2 in Tibetans. Subsequent association analyses of hemoglobin levels in two independent Tibetan populations (a total of 1,250 individuals) showed a male-specific association between the HMOX2 variants and hemoglobin levels. Tibetan males with the derived C allele at rs4786504:T>C displayed lower hemoglobin level as compared with the T allele carriers. Furthermore, our in vitro experiments indicated that the C allele of rs4786504 could increase the expression of HMOX2, presumably leading to a more efficient breakdown of heme that may help maintain a relatively low hemoglobin level at high altitude. Collectively, we propose that HMOX2 contributes to high-altitude adaptation in Tibetans by functioning as a modifier in the regulation of hemoglobin metabolism.
Assuntos
Adaptação Fisiológica/genética , Heme Oxigenase (Desciclizante)/genética , Hemoglobinas/genética , Hipóxia/genética , Polimorfismo de Nucleotídeo Único , Adulto , Alelos , Altitude , Etnicidade , Éxons , Feminino , Regulação da Expressão Gênica , Frequência do Gene , Heme/metabolismo , Heme Oxigenase (Desciclizante)/metabolismo , Hemoglobinas/metabolismo , Humanos , Hipóxia/etnologia , Hipóxia/metabolismo , Masculino , Pessoa de Meia-Idade , Análise de Sequência de DNA , Fatores Sexuais , Transdução de Sinais , TibetRESUMO
Tibetans live on the highest plateau in the world, their current population size is approximately 5 million, and most of them live at an altitude exceeding 3,500 m. Therefore, the Tibetan Plateau is a remarkable area for cultural and biological studies of human population history. However, the chronological profile of the Tibetan Plateau's colonization remains an unsolved question of human prehistory. To reconstruct the prehistoric colonization and demographic history of modern humans on the Tibetan Plateau, we systematically sampled 6,109 Tibetan individuals from 41 geographic populations across the entire region of the Tibetan Plateau and analyzed the phylogeographic patterns of both paternal (n = 2,354) and maternal (n = 6,109) lineages as well as genome-wide single nucleotide polymorphism markers (n = 50) in Tibetan populations. We found that there have been two distinct, major prehistoric migrations of modern humans into the Tibetan Plateau. The first migration was marked by ancient Tibetan genetic signatures dated to approximately 30,000 years ago, indicating that the initial peopling of the Tibetan Plateau by modern humans occurred during the Upper Paleolithic rather than Neolithic. We also found evidences for relatively young (only 7-10 thousand years old) shared Y chromosome and mitochondrial DNA haplotypes between Tibetans and Han Chinese, suggesting a second wave of migration during the early Neolithic. Collectively, the genetic data indicate that Tibetans have been adapted to a high altitude environment since initial colonization of the Tibetan Plateau in the early Upper Paleolithic, before the last glacial maximum, followed by a rapid population expansion that coincided with the establishment of farming and yak pastoralism on the Plateau in the early Neolithic.
Assuntos
Povo Asiático/genética , Dinâmica Populacional , Cromossomos Humanos Y , DNA Mitocondrial/genética , Emigração e Imigração , Evolução Molecular , Feminino , Variação Genética , Geografia , Haplótipos , Humanos , Masculino , Polimorfismo de Nucleotídeo Único , TibetRESUMO
Tibetans are well adapted to high-altitude hypoxic conditions, and in recent genome-wide scans, many candidate genes have been reported involved in the physiological response to hypoxic conditions. However, the limited sequence variations analyzed in previous studies would not be sufficient to identify causal mutations. Here we conducted resequencing of the entire genomic region (59.4 kb) of the hypoxic gene EGLN1 (one of the top candidates from the genome-wide scans) in Tibetans and identified 185 sequence variations, including 13 novel variations (12 substitutions and 1 insertion or deletion). There is a nonsynonymous mutation (rs186996510, D4E) showing surprisingly deep divergence between Tibetans and lowlander populations (Fst = 0.709 between Tibetans and Han Chinese). It is highly prevalent in Tibetans (70.9% on average) but extremely rare in Han Chinese, Japanese, Europeans, and Africans (0.56-2.27%), suggesting that it might be the causal mutation of EGLN1 contributing to high-altitude hypoxic adaptation. Neutrality test confirmed the signal of Darwinian positive selection on EGLN1 in Tibetans. Haplotype network analysis revealed a Tibetan-specific haplotype, which is absent in other world populations. The estimated selective intensity (0.029 for the C allele of rs186996510) puts EGLN1 among the known genes that have undergone the strongest selection in human populations, and the onset of selection was estimated to have started at the early Neolithic (â¼8,400 years ago). Finally, we detected a significant association between rs186996510 and hemoglobin levels in Tibetans, suggesting that EGLN1 contributes to the adaptively low hemoglobin level of Tibetans compared with acclimatized lowlanders at high altitude.
Assuntos
Aclimatação/genética , Altitude , Povo Asiático/genética , Prolina Dioxigenases do Fator Induzível por Hipóxia/genética , Hipóxia/genética , Mutação , Adulto , Alelos , Feminino , Frequência do Gene , Estudos de Associação Genética , Haplótipos , Hemoglobinas/metabolismo , Humanos , Desequilíbrio de Ligação , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Consumo de Oxigênio , Fenótipo , Polimorfismo de Nucleotídeo Único , Seleção Genética , TibetRESUMO
BACKGROUND: One of the key genes that regulate human brain size, MCPH1 has evolved under strong Darwinian positive selection during the evolution of primates. During this evolution, the divergence of MCPH1 protein sequences among primates may have caused functional changes that contribute to brain enlargement. RESULTS: To test this hypothesis, we used co-immunoprecipitation and reporter gene assays to examine the activating and repressing effects of MCPH1 on a set of its down-stream genes and then compared the functional outcomes of a series of mutant MCPH1 proteins that carry mutations at the human- and great-ape-specific sites. The results demonstrate that the regulatory effects of human MCPH1 and rhesus macaque MCPH1 are different in three of eight down-stream genes tested (p73, cyclinE1 and p14ARF), suggesting a functional divergence of MCPH1 between human and non-human primates. Further analyses of the mutant MCPH1 proteins indicated that most of the human-specific mutations could change the regulatory effects on the down-stream genes. A similar result was also observed for one of the four great-ape-specific mutations. CONCLUSIONS: Collectively, we propose that during primate evolution in general and human evolution in particular, the divergence of MCPH1 protein sequences under Darwinian positive selection led to functional modifications, providing a possible molecular mechanism of how MCPH1 contributed to brain enlargement during primate evolution and human origin.
Assuntos
Evolução Biológica , Encéfalo/anatomia & histologia , Variação Genética , Macaca mulatta/genética , Proteínas do Tecido Nervoso/genética , Sequência de Aminoácidos , Substituição de Aminoácidos/genética , Animais , Proteínas de Ciclo Celular , Proteínas do Citoesqueleto , Fator de Transcrição E2F1/metabolismo , Regulação da Expressão Gênica , Humanos , Dados de Sequência Molecular , Proteínas Mutantes/metabolismo , Mutação/genética , Proteínas do Tecido Nervoso/química , Tamanho do Órgão/genética , Filogenia , Mapeamento de Interação de Proteínas , Alinhamento de Sequência , Especificidade da Espécie , Transcrição GênicaRESUMO
Compared with lowlander migrants, native Tibetans have a higher reproductive success at high altitude though the underlying mechanism remains unclear. Here, we compared the transcriptome and histology of full-term placentas between native Tibetans and Han migrants. We found that the placental trophoblast shows the largest expression divergence between Tibetans and Han, and Tibetans show decreased immune response and endoplasmic reticulum stress. Remarkably, we detected a sex-biased expression divergence, where the male-infant placentas show a greater between-population difference than the female-infant placentas. The umbilical cord plays a key role in the sex-biased expression divergence, which is associated with the higher birth weight of the male newborns of Tibetans. We also identified adaptive histological changes in the male-infant placentas of Tibetans, including larger umbilical artery wall and umbilical artery intima and media, and fewer syncytial knots. These findings provide valuable insights into the sex-biased adaptation of human populations, with significant implications for medical and genetic studies of human reproduction.
Assuntos
Desenvolvimento Fetal , Placenta , Humanos , Feminino , Placenta/metabolismo , Masculino , Gravidez , Desenvolvimento Fetal/genética , Tibet , Recém-Nascido , Transcriptoma , Altitude , Fatores Sexuais , Caracteres SexuaisRESUMO
A novel UV/oxalic acid functionalized corn straw biochar (OCBC)/peroxyacetic acid (PAA) system was built to degrade sulfadiazine from waters. 94.7 % of SDZ was removed within 30 min by UV/OCBC/PAA. The abundant surface functional groups and persistent free radicals (PFRs) on OCBC were responsible for these performances. Cyclic voltammetry (CV) and other characterization analysis revealed, under UV irradiation, the addition of OCBC served as electron donor, which might promote the reaction of electrons with PAA. The quenching and electron paramagnetic resonance (EPR) tests indicated that R-Oâ¢, 1O2 and â¢OH were generated. Theoretical calculations indicated sulfonamide bridge was vulnerable under the attacks of reactive species. In addition, high removal effect achieved by 5 reuse cycles and different real waters also suggested the sustainability of UV/OCBC/PAA. Overall, this study provided a feasible approach to remove SDZ with high mineralization efficiency, in addition to a potential strategy for resource utilization of corn straw.
Assuntos
Carvão Vegetal , Ácido Oxálico , Ácido Peracético , Sulfadiazina , Raios Ultravioleta , Zea mays , Zea mays/química , Carvão Vegetal/química , Ácido Peracético/química , Sulfadiazina/química , Ácido Oxálico/química , Transporte de Elétrons , Poluentes Químicos da Água , Purificação da Água/métodosRESUMO
The Qinghai-Tibet Plateau is a valuable genetic resource pool, and the high-altitude adaptation of Tibetan pigs is a classic example of the adaptive evolution of domestic animals. Here, we report the presence of Darwinian positive selection signatures in Tibetan pigs (TBPs) using 348 genome-wide datasets (127 whole-genome sequence datasets (WGSs) and 221 whole-genome single-nucleotide polymorphism (SNP) chip datasets). We characterized a high-confidence list of genetic signatures related response to high-altitude adaptation in Tibetan pigs, including 4,598 candidate SNPs and 131 candidate genes. Functional annotation and enrichment analysis revealed that 131 candidate genes are related to multiple systems and organs in Tibetan pigs. Notably, eight of the top ten novel genes, RALB, NBEA, LIFR, CLEC17A, PRIM2, CDH7, GK5 and FAM83B, were highlighted and associated with improved adaptive heart functions in Tibetan pigs high-altitude adaptation. Moreover, genome-wide association analysis revealed that 29 SNPs were involved in 13 candidate genes associated with at least one adaptive trait. In particular, among the top ten candidate genes, CLEC17A is related to a reduction in hemoglobin (HGB) in Tibetan pigs. Overall, our study provides a robust SNP/gene list involving genetic adaptation for Tibetan pig high-altitude adaptation, and it will be a valuable resource for future Tibetan pig studies.
Assuntos
Altitude , Estudo de Associação Genômica Ampla , Polimorfismo de Nucleotídeo Único , Seleção Genética , Animais , Tibet , Suínos/genética , Adaptação Fisiológica/genética , Genoma , Sequenciamento Completo do GenomaRESUMO
BACKGROUND: The miR-513 subfamily belongs to an X-linked primate-specific miR506-514 cluster. Across primate species, there have been several duplication events and different species each possess a variety of miR-513 copies, indicating it underwent rapid evolution. Evidence suggests that this subfamily is preferentially expressed in the testis, but otherwise, to date, the evolutionary history and functional significance of this miRNA subfamily has remained largely unexplored. RESULTS: We analyzed the evolutionary pattern of gene duplications and their functional consequence for the miR-513 subfamily in primates. Sequence comparisons showed that the duplicated copies of miR-513 were derived from transposable element (MER91C). Moreover, duplication events of the miR-513 subfamily seem to have occurred independently in Platyrrhini (New World monkeys) and Catarrhini (Old World monkeys, apes and humans) after they diverged. Different copies of the miR-513 subfamily (miR-513a/b/c) have different seed sequences, due to after-duplication sequence divergences, which eventually led to functional divergences. The results of functional assays indicated that miR-513b could inhibit the expression of its target gene, the down-regulator of transcription 1 (DR1) at both the mRNA and protein levels. In the developing testis of rhesus macaques, we observed a temporal coupling of expression levels between miR-513b and DR1, suggesting that miR-513b could affect male sexual maturation by negatively regulating the development-stage related functioning of DR1. CONCLUSIONS: The miR-513 subfamily underwent multiple independent gene duplications among five different lineages of primates. The after-duplication sequence divergences among the different copies of miR-513 led to functional divergence of these copies in primates.