Ecology and Quality Suitability Regionalization of Sabia parviflora / 中国实验方剂学杂志

Objective:To explore the potential suitable distribution area and the high-quality distribution area of <italic>Sabia parviflora</italic>. Method:Combined with the distribution information and environmental factors，the maximum entropy （MaxEnt） model and ArcGIS software were used to predict the potential suitable distribution area of <italic>S. parviflora</italic>. Based on the correlation between environmental factors and total saponins，total flavonoids，quercetin-3-<italic>O</italic>-gentiobioside，camellianoside，tsubakioside A，kaempferol-3-<italic>O</italic>-rutinoside and isobariclisin-3-<italic>O</italic>-rutinoside，the quality regionalization was conducted by using spatial interpolation method and fuzzy superposition function in ArcGIS software. Result:<italic>S. parviflora</italic> is mainly distributed in Yunnan，Guizhou，Guangxi province in China. The medium and high suitable areas accounts for about 2.88% of the national area. The precipitation in October and November，the precipitation in the warmest and driest seasons，the standard deviation of seasonal changes in temperature and altitude are the main environmental factors that affect the distribution of <italic>S. parviflora</italic>. Slope，precipitation，solar radiation and temperature change had great influence on the accumulation of secondary metabolites. Based on the results of potential suitable distribution and spatial interpolation of each component，the high-quality areas of <italic>S. parviflora</italic> are mainly concentrated in the southwest of Guizhou，with Qinglong，Guanling，Zhenning，Pu'an，Xingren county and other areas as the core. Conclusion:This study provides a scientific guidance for the site selection of artificial planting and the procurement of medicinal materials for <italic>S. parviflora</italic>.

Effect of modified Buzhong Yiqi decoction combined with pelvic floor muscle exercise-biofeedback-electrical stimulation on early stage postpartum pelvic floor dysfunction / 中国中药杂志

To investigate the curative effect of modified Buzhong Yiqi decoction combined with pelvic floor muscle exercise-biofeedback-electrostimulation on early postpartum pelvic floor dysfunction disease (PFD) and its effect on the expression of transforming growth factor (TGF-β1), matrix metalloprotein (MMP-2), and tissue inhibitor of metalloproteinase (TIMP-2). A total of 186 PFD patients admitted to our hospital from March 2014 to December 2016 were selected in the study and were randomly divided into test group (=93) and control group (=93). The control group received pelvic floor muscle exercises-biofeedback-electrostimulation, while the test group was additionally treated with modified Buzhong Yiqi decoction based on the treatment in control group. Then the clinical efficacy was compared between two groups. The results showed that vaginal contractile electromyography (EMG), duration of vaginal constriction, dynamic vaginal pressure and pelvic floor myoelectric activity in the test group at 1, 3 and 6 months after treatment were significantly higher than those before treatment and the control group (<0.05). The effective rate for urinary incontinence was 97.14% in test group, significantly higher than 75.68% in the control group (<0.05). The effective rate for improvement of sexual life quality was 96.43% in test group, significantly higher than 74.07% in control group (<0.05). 1, 3 and 6 months after treatment, the uterine prolapse, posterior wall prolapse and anterior wall prolapse grade in test group was slightly lower than that in the control group, but the difference was not statistically significant. After treatment, the levels of TGF-β1 and TIMP-2 in the test group were higher than those in the control group, while the levels of MMP-2 were lower than those in the control group (<0.05). The results showed that modified Buzhong Yiqi decoction combined with pelvic floor muscle exercise-biofeedback-electrostimulation can effectively relieve the clinical symptoms, promote the body recovery and improve the quality of sexual life in PFD patients, with remarkable advantages, so it is worthy of popularizing.

Development of male gametophyte of Larix leptolepis Gord. with emphasis on diffuse stage of meiosis.

A basic developmental framework of the Larix leptolepis Gord male gametophyte is presented in detail by squashing technique. The duration of the meiosis stage was more than 6 months, and included a long diffuse stage during winter. This long duration of the diffuse appearance of the diplotene stage makes L. leptolepis a unique suitable experimental material for studying the structure and function of the diffuse stage of meiosis. In particular, the processes of desynapsing and unpairing, which so far have received little attention, can be examined in detail. In L. leptolepis, the chromosomes undergo a dramatic structural reorganization during the diffuse diplotene stage. Based on the clearly visible differences in chromosome morphology, the diffuse diplotene stage was divided into four periods with suggested nomenclature as follows: schizonema, pre-diffuse diplotene, diffuse diplotene and post-diffuse diplotene. Both simultaneous and successive microsporogenesis were observed within L. leptolepis, and there was no strict relationship between the microsporogenesis types and the tetrad configurations, which are strongly influenced by spindle orientation, especially during meiosis II. The mature pollen grain at pollination consists of five cells aligned in an axial row. The prothallial cells cannot be regarded as senescent cells because they remain capable of division.