Double-hit lymphomas: clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma.

BACKGROUND: Double-hit lymphomas (DHL) are rare high-grade neoplasms characterized by two translocations: one involving the gene MYC and another involving genes BCL2 or BCL6, whose diagnosis depends on cytogenetic examination. This research studied DHL and morphological and/or immunophenotypic factors associated with the detection of these translocations in a group of high-grade non-Hodgkin lymphoma cases. METHOD: Clinical and morphological reviews of 120 cases diagnosed with diffuse large B-cell lymphoma and Burkitt lymphoma were conducted. Immunohistochemistry (CD20, CD79a, PAX5, CD10, Bcl6, Bcl2, MUM1, TDT and Myc) and fluorescence in situ hybridization for detection of MYC, BCL2 and BCL6 gene translocations were performed in a tissue microarray platform. RESULTS: Three cases of DHL were detected: two with translocations of MYC and BCL2 and one with translocations of MYC and BCL6, all leading to death in less than six months. Among 90 cytogenetically evaluable biopsies, associations were determined between immunohistochemistry and fluorescence in situ hybridization for MYC (p = 0.036) and BCL2 (p = 0.001). However, these showed only regular agreement, indicated by Kappa values of 0.23 [0.0;0.49] and 0.35 [0.13;0.56], respectively. "Starry sky" morphology was strongly associated with MYC positivity (p = 0.01). The detection of three cases of DHL, all resulting in death, confirms the rarity and aggressiveness of this neoplasm. CONCLUSIONS: The "starry sky" morphological pattern and immunohistochemical expression of Myc and Bcl2 represent possible selection factors for additional cytogenetic diagnostic testing.

Dipyrone has no effects on bone healing of tibial fractures in rats.

OBJECTIVE: To evaluate the effect of dipyrone on healing of tibial fractures in rats. METHODS: Fourty-two Wistar rats were used, with mean body weight of 280g. After being anesthetized, they were submitted to closed fracture of the tibia and fibula of the right posterior paw through manual force. The rats were randomly divided into three groups: the control group that received a daily intraperitoneal injection of saline solution; group D-40, that received saline injection containing 40mg/Kg dipyrone; and group D-80, that received saline injection containing 80mg/Kg dipyrone. After 28 days the rats were sacrificed and received a new label code that was known by only one researcher. The fractured limbs were then amputated and X-rayed. The tibias were disarticulated and subjected to mechanical, radiological and histological evaluation. For statistical analysis the Kruskal-Wallis test was used at a significance level of 5%. RESULTS: There wasn't any type of dipyrone effect on healing of rats tibial fractures in relation to the control group. CONCLUSION: Dipyrone may be used safely for pain control in the treatment of fractures, without any interference on bone healing. Level of Evidence II, Controlled Laboratory Study.

Dipirona não tem efeito sobre consolidação das fraturas da tíbia de ratos / Dipyrone has no effects on bone healing of tibial fractures in rats

Avaliar os efeitos da dipirona na consolidação de fraturasda tíbia de ratos. Métodos: Foram utilizados 42 ratos da linhagem Wistar, com peso médio de 280 g. Após serem anestesiados, osanimais foram submetidos à fratura da tíbia e fíbula da pata posteriordireita por força manual. Os ratos foram divididos, por sorteio, emtrês grupos: o grupo-controle, que recebeu uma injeção intraperitonealdiária de solução salina; o grupo D-40, que recebeu soluçãosalina com dipirona na concentração de 40 mg/Kg; e o grupo D-80,que recebeu solução salina contendo dipirona na concentração de80 mg/Kg. Após 28 dias os ratos foram sacrificados e receberam novacodificação, conhecida apenas por um dos pesquisadores. Os membrosfraturados foram então amputados e radiografados. As tíbiasforam desarticuladas e submetidas à avaliação mecânica, radiográficae histológica. Na análise estatística foi utilizado o teste de Kruskal--Wallis e estabelecido o nível de significância de 5%. Resultados: Nãofoi verificado nenhum tipo de efeito da dipirona na consolidação dasfraturas das tíbias dos ratos estudados em relação ao grupo controle.Conclusão: Dipirona pode ser utilizada com segurança no controleda dor no tratamento de fraturas, sem interferência na consolidaçãoóssea. Nível de Evidência II, Estudo Laboratorial Controlado...

To evaluate the effect of dipyrone on healing of tibialfractures in rats. Methods: Fourty-two Wistar rats were used, withmean body weight of 280 g. After being anesthetized, they weresubmitted to closed fracture of the tibia and fibula of the rightposterior paw through manual force. The rats were randomlydivided into three groups: the control group that received a dailyintraperitoneal injection of saline solution; group D-40, thatreceived saline injection containing 40 mg/Kg dipyrone; andgroup D-80, that received saline injection containing 80 mg/Kgdipyrone. After 28 days the rats were sacrificed and receiveda new label code that was known by only one researcher. Thefractured limbs were then amputated and X-rayed. The tibiaswere disarticulated and subjected to mechanical, radiologicaland histological evaluation. For statistical analysis the Kruskal--Wallis test was used at a significance level of 5%. Results:There wasn’t any type of dipyrone effect on healing of rats tibialfractures in relation to the control group. Conclusion: Dipyronemay be used safely for pain control in the treatment of fractures,without any interference on bone healing. Level of Evidence II,Controlled Laboratory Study...

Adenomioepitelioma de mama: relato de caso / Breast adenomyoepithelioma: case report

O adenomioepitelioma é uma lesão cuja terminologia sugere ser um tumor benigno, mas observado na literatura médica com potencialidade para o aparecimento de metástases, principalmente em gânglios axilares. Os autores apresentam um caso ocorrido após lactação. A lesão foi tratada com cirurgia local e a paciente continua em segmento contínuo.

Cistite intersticial - experiencia de 12 casos tratados com dimityl-sulfoxide (DMSO) / Interstitial cystitis: experience with twelve patients treated with dimetyl-sulfoxide (DMSO)

Os autores apresentam sua experiencia no tratamento da cistite intersticial com Dimetyl-Sulfoxide (DMSO). Doze pacientes com cistite intersticial diagnosticada atraves da historia, aspecto cistoscopico e biopsia da mucosa vesical foram tratados com DMSO. O desaparecimento dos sintomas verificou-se em 83 por cento das pacientes que completaram o tratamento, nao sendo constatados efeitos colaterais significativos