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1.
Nat Biotechnol ; 22(5): 547-53, 2004 May.
Artigo em Inglês | MEDLINE | ID: mdl-15064768

RESUMO

Thermus thermophilus HB27 is an extremely thermophilic, halotolerant bacterium, which was originally isolated from a natural thermal environment in Japan. This organism has considerable biotechnological potential; many thermostable proteins isolated from members of the genus Thermus are indispensable in research and in industrial applications. We present here the complete genome sequence of T. thermophilus HB27, the first for the genus Thermus. The genome consists of a 1,894,877 base pair chromosome and a 232,605 base pair megaplasmid, designated pTT27. The 2,218 identified putative genes were compared to those of the closest relative sequenced so far, the mesophilic bacterium Deinococcus radiodurans. Both organisms share a similar set of proteins, although their genomes lack extensive synteny. Many new genes of potential interest for biotechnological applications were found in T. thermophilus HB27. Candidates include various proteases and key enzymes of other fundamental biological processes such as DNA replication, DNA repair and RNA maturation.


Assuntos
Genoma Bacteriano , Thermus thermophilus/genética , Dados de Sequência Molecular , Plasmídeos
2.
J Mol Biol ; 321(1): 149-62, 2002 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-12139940

RESUMO

4-alpha-Glucanotransferase (GTase) is an essential enzyme in alpha-1,4-glucan metabolism in bacteria and plants. It catalyses the transfer of maltooligosaccharides from an 1,4-alpha-D-glucan molecule to the 4-hydroxyl group of an acceptor sugar molecule. The crystal structures of Thermotoga maritima GTase and its complex with the inhibitor acarbose have been determined at 2.6A and 2.5A resolution, respectively. The GTase structure consists of three domains, an N-terminal domain with the (beta/alpha)(8) barrel topology (domain A), a 65 residue domain, domain B, inserted between strand beta3 and helix alpha6 of the barrel, and a C-terminal domain, domain C, which forms an antiparallel beta-structure. Analysis of the complex of GTase with acarbose has revealed the locations of five sugar-binding subsites (-2 to +3) in the active-site cleft lying between domain B and the C-terminal end of the (beta/alpha)(8) barrel. The structure of GTase closely resembles the family 13 glycoside hydrolases and conservation of key catalytic residues previously identified for this family is consistent with a double-displacement catalytic mechanism for this enzyme. A distinguishing feature of GTase is a pair of tryptophan residues, W131 and W218, which, upon the carbohydrate inhibitor binding, form a remarkable aromatic "clamp" that captures the sugar rings at the acceptor-binding sites +1 and +2. Analysis of the structure of the complex shows that sugar residues occupying subsites from -2 to +2 engage in extensive interactions with the protein, whereas the +3 glucosyl residue makes relatively few contacts with the enzyme. Thus, the structure suggests that four subsites, from -2 to +2, play the dominant role in enzyme-substrate recognition, consistent with the observation that the smallest donor for T.maritima GTase is maltotetraose, the smallest chain transferred is a maltosyl unit and that the smallest residual fragment after transfer is maltose. A close similarity between the structures of GTase and oligo-1,6-glucosidase has allowed the structural features that determine differences in substrate specificity of these two enzymes to be analysed.


Assuntos
Acarbose/metabolismo , Sistema da Enzima Desramificadora do Glicogênio/química , Sistema da Enzima Desramificadora do Glicogênio/metabolismo , Thermotoga maritima/enzimologia , Acarbose/química , Acarbose/farmacologia , Sítios de Ligação , Cálcio/metabolismo , Sequência de Carboidratos , Catálise , Cristalografia por Raios X , Inibidores Enzimáticos/química , Inibidores Enzimáticos/metabolismo , Inibidores Enzimáticos/farmacologia , Sistema da Enzima Desramificadora do Glicogênio/antagonistas & inibidores , Ligação de Hidrogênio , Modelos Moleculares , Dados de Sequência Molecular , Oligo-1,6-Glucosidase/química , Oligo-1,6-Glucosidase/metabolismo , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Subunidades Proteicas , Especificidade por Substrato
3.
FEBS Lett ; 517(1-3): 267-71, 2002 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-12062450

RESUMO

The NAD+-requiring enzymes of glycoside hydrolase family 4 (GHF4) contain a region with a conserved Gly-XXX-Gly-Ser (GXGS) motif near their N-termini that is reminiscent of the fingerprint region of the Rossmann fold, a conserved structural motif of classical nicotinamide nucleotide-binding proteins. The function of this putative NAD+-binding motif in the alpha-glucosidase AglA of Thermotoga maritima was probed by directed mutagenesis. The K(d) for NAD+ of the AglA mutants G10A, G12A and S13A was increased by about 300-, 5-, and 9-fold, respectively, while their K(m) for p-nitrophenyl-alpha-glucopyranoside was not seriously affected. The results indicate that the GXGS motif is indeed important for NAD+ binding by the glycosidases of GHF4.


Assuntos
NAD/metabolismo , Thermotoga maritima/enzimologia , alfa-Glucosidases/metabolismo , Motivos de Aminoácidos/genética , Motivos de Aminoácidos/fisiologia , Sequência de Aminoácidos , Sítios de Ligação/genética , Sítios de Ligação/fisiologia , Sequência Conservada , Cinética , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Thermotoga maritima/genética , alfa-Glucosidases/química , alfa-Glucosidases/genética
4.
J Bacteriol ; 186(16): 5432-41, 2004 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-15292145

RESUMO

Nonpathogenic Escherichia coli strain Nissle 1917 (O6:K5:H1) is used as a probiotic agent in medicine, mainly for the treatment of various gastroenterological diseases. To gain insight on the genetic level into its properties of colonization and commensalism, this strain's genome structure has been analyzed by three approaches: (i) sequence context screening of tRNA genes as a potential indication of chromosomal integration of horizontally acquired DNA, (ii) sequence analysis of 280 kb of genomic islands (GEIs) coding for important fitness factors, and (iii) comparison of Nissle 1917 genome content with that of other E. coli strains by DNA-DNA hybridization. PCR-based screening of 324 nonpathogenic and pathogenic E. coli isolates of different origins revealed that some chromosomal regions are frequently detectable in nonpathogenic E. coli and also among extraintestinal and intestinal pathogenic strains. Many known fitness factor determinants of strain Nissle 1917 are localized on four GEIs which have been partially sequenced and analyzed. Comparison of these data with the available knowledge of the genome structure of E. coli K-12 strain MG1655 and of uropathogenic E. coli O6 strains CFT073 and 536 revealed structural similarities on the genomic level, especially between the E. coli O6 strains. The lack of defined virulence factors (i.e., alpha-hemolysin, P-fimbrial adhesins, and the semirough lipopolysaccharide phenotype) combined with the expression of fitness factors such as microcins, different iron uptake systems, adhesins, and proteases, which may support its survival and successful colonization of the human gut, most likely contributes to the probiotic character of E. coli strain Nissle 1917.


Assuntos
Escherichia coli/genética , Genoma Bacteriano , Adesinas Bacterianas/genética , Adesinas Bacterianas/metabolismo , Bacteriocinas/genética , Bacteriocinas/metabolismo , DNA Bacteriano/análise , DNA Bacteriano/isolamento & purificação , Endopeptidases/genética , Endopeptidases/metabolismo , Enterobactina/metabolismo , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Transferência Genética Horizontal , Ilhas Genômicas , Proteínas Hemolisinas/genética , Proteínas Hemolisinas/metabolismo , Ferro/metabolismo , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Polissacarídeos Bacterianos/biossíntese , Análise de Sequência de DNA
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