Biodiversity and ecosystem functioning relations in European forests depend on environmental context.

The importance of biodiversity in supporting ecosystem functioning is generally well accepted. However, most evidence comes from small-scale studies, and scaling-up patterns of biodiversity-ecosystem functioning (B-EF) remains challenging, in part because the importance of environmental factors in shaping B-EF relations is poorly understood. Using a forest research platform in which 26 ecosystem functions were measured along gradients of tree species richness in six regions across Europe, we investigated the extent and the potential drivers of context dependency of B-EF relations. Despite considerable variation in species richness effects across the continent, we found a tendency for stronger B-EF relations in drier climates as well as in areas with longer growing seasons and more functionally diverse tree species. The importance of water availability in driving context dependency suggests that as water limitation increases under climate change, biodiversity may become even more important to support high levels of functioning in European forests.

Breaking seed dormancy of three orthodox Mediterranean Rosaceae species.

Biodiversity levels could be enhanced when regenerating a site by seed-derived seedlings. However, seed dormancy poses limitations for many species. As a result, nurseries either produce seedlings from species where dormancy is not an obstacle, or they propagate through cuttings with the risk of decreasing the genetic diversity within and among species at the regenerated sites. In the present study, breaking of seed dormancy was investigated in valuable Mediterranean species of Prunus avium, Prunus spinosa and Rosa canina Specifically, in order to break dormancy, seeds of those species were warm-, cold-stratified and chemically treated. Based on the results, maximum germination for P. avium was 12% when seeds were warm stratified for four weeks altered with eight weeks of cold stratification. For P. spinosa, maximum percent germination was 26% when seeds were warm stratified for two weeks and continuously altered for eight weeks of cold stratification. Finally, for R. canina maximum percent germination was 40% under four weeks of warm stratification altered with twenty weeks of cold stratification, when seeds were pretreated with H2SO4 for 15 min. A maximum of twelve weeks of cold stratification for P. avium, P. spinosa and 20 weeks for R. canina provided almost zero percent germination. The results indicated that all three species experienced intense dormancy levels suggesting that those species need to be treated properly prior to sowing. Nonetheless, additional experiments are needed to achieve greater germination percentage of highly valuable species in orderto encourage seed derived seedling production.

Physiological and phenological adjustments in water and carbon fluxes of Aleppo pine forests under contrasting climates in the Eastern Mediterranean.

The ability of plants to adjust to the adverse effects of climate change is important for their survival and for their contribution to the global carbon cycle. This is particularly true in the Mediterranean region, which is among the regions that are most vulnerable to climate change. Here, we carried out a 2-year comparative ecophysiological study of ecosystem function in two similar Eastern Mediterranean forests of the same tree species (Pinus halepensis Mill.) under mild (Sani, Greece) and extreme (Yatir, Israel) climatic conditions. The partial effects of key environmental variables, including radiation, vapor pressure deficit, air temperature and soil moisture (Rg, D, T and soil water content (SWC), respectively), on the ecosystems' CO2 and water vapor fluxes were estimated using generalized additive models (GAMs). The results showed a large adjustment between sites in the seasonal patterns of both carbon and water fluxes and in the time and duration of the optimal period (defined here as the time when fluxes were within 85% of the seasonal maximum). The GAM analysis indicated that the main factor influencing the seasonal patterns was SWC, while T and D had significant but milder effects. During the respective optimal periods, the two ecosystems showed strong similarities in the fluxes' responses to the measured environmental variables, indicating similarity in their underlying physiological characteristics. The results indicate that Aleppo pine forests have a strong phenotypic adjustment potential to cope with increasing environmental stresses. This, in turn, will help their survival and their continued contribution to the terrestrial carbon sink in the face of climate change in this region.

Corrigendum to "Within-forest stand (or formation, or plot) and between-forest stand (or formation, or plot) biodiversity indices" [MethodsX 9 (2022) 1-6/101919].

[This corrects the article DOI: 10.1016/j.mex.2022.101919.].

Applying taper function models for black locust plantations in Greek post-mining areas.

A key process in forest management planning is the estimation of tree volume and, more specifically, merchantable volume. The ability to predict the cumulative stem volume relative to any upper stem diameter on standing trees or stands is essential for forest inventories and the management of forest resources. In the 1980s, the Hellenic Public Power Corporation (HPPC) started the rehabilitation of lignite post-mining areas in Greece by planting mainly black locust (Robinia pseudoacacia, L.). Today, these plantations occupy an area of approximately 2570 ha, but the stem volume has not yet been estimated. Therefore, we aimed to estimate the over- and under-bark stem volume using taper function models for 30 destructively sampled trees. Of the nineteen calibrated fixed-effects models, Kozak's (2004) equation performed best for both the over-bark and under-bark datasets, followed by Lee's (2003) and Muhairwe's (1999) equations. Two fixed effect models were compared with fitted coefficients from Poland and the United States confirming that the local model fits were better suited, as the foreign model coefficients caused an increase in root mean square error (RMSE) for stem diameter predictions of 13% and 218%, respectively. The addition of random effects on a single-stem basis for two coefficients of Kozak's (2004) equation improved the model fit significantly at 86% of the over-bark fixed effect RMSE and 69% for the under-bark model. Integrated taper functions were found to slightly outperform three volume equations for predictions of single stem volume over and under bark. Ultimately it was shown that these models can be used to precisely predict stem diameters and total stem volume for the population average as well as for specific trees of the black locust plantations in the study area.

Within-forest stand (or formation, or plot) and between-forest stand (or formation, or plot) biodiversity indices.

The idea of entropy, which has its roots in information theory and proposes that one may measure the degree of uncertainty associated with the prediction of bits and pieces of information, has been widely used by biologists and ecologists for decades to define biological diversity. For ecologists, the core of the issue is whether or not two species' distribution taken from the same habitat are the same or distinct. The Shannon index and Simpson diversity are well-known in ecology; however, the non-linearity of these indices may cause a misinterpretation of the underlying diversity, as shown by Lou Jost (2006) and others. Applying the proposed template, one can:•calculate several biodiversity indices,•compare two different forest stands (or formations, or plots), or two different profiles in two different times, for the same forest stand (or formation, or plot), in terms of biodiversity.

Effective Defense of Aleppo Pine Against the Giant Scale Marchalina hellenica Through Ecophysiological and Metabolic Changes.

Aleppo pine (Pinus halepensis) is widely distributed in the Mediterranean region and in other areas of the world, where it has been introduced due to its adaptive capacity to xerothermic conditions. The giant pine scale Marchalina hellenica often infests Aleppo pine, as well as other pines, in several southeastern European countries, causing pine declines. When combined with the expected intensified heat and drought events in eastern Mediterranean, the impact of this biotic parameter on the host pines may be exacerbated. The importance of understanding the defense mechanisms of Aleppo pine is emphasized by the recent invasion of the pine scale in new regions, like Australia, lacking the insect's natural enemies, where more intense negative effects on pine species may occur. To date, Aleppo pine's physiological responses to the infestation by M. hellenica are largely unknown. This study aimed at assessing the responses of Aleppo pine to the giant pine scale attack, both on an ecophysiological and a metabolic level. For this purpose, gas exchange, needle water status, and carbon and nitrogen content were measured during 1 year on healthy and infested adult trees. M etabolic profiling of Aleppo pine needles was also performed before, during, and after the high feeding activity of the insect. The maintenance of stable relative water content, δ13C signatures, and chlorophyll fluorescence in the needles of infested pines indicated that infestation did not induce drought stress to the host pines. At the peak of infestation, stomatal closure and a pronounced reduction in assimilation were observed and were associated with the accumulation of sugars in the needles, probably due to impaired phloem loading. At the end of the infestation period, tricarboxylic acids were induced and phenolic compounds were enhanced in the needles of infested pines. These metabolic responses, together with the recovery of photosynthesis after the end of M. hellenica intense feeding, indicate that in the studied region and under the current climate, Aleppo pine is resilient to the infestation by the giant pine scale. Future research should assess whether these promising defense mechanisms are also employed by other host pines, particularly in regions of the world recently invaded by the giant pine scale, as well as under more xerothermic regimes.

Short-term effects of thinning on soil CO2, N2O and CH4 fluxes in Mediterranean forest ecosystems.

In Mediterranean ecosystems an increasing demand for in situ trace gas exchange data is emerging to enhance the adaptation and mitigation strategies under forest degradation. Field-chamber green-house gas fluxes and site characteristics were analysed in two Mediterranean peri-urban pine forests showing degradation symptoms. We examined the effect of different thinning interventions on soil CO2, CH4 and N2O fluxes, addressing the relationships with the environmental variables and C and N contents along forest floor-soil layers. Soil temperature resulted as the main driving variable for CO2 efflux and CH4 uptake. Soil moisture content and organic matter availability affected CO2 emission patterns in the two sites. N2O fluxes showed a positive correlation with soil moisture under wetter climatic conditions only. GHG fluxes showed significant correlations with C and N content of both forest floor and mineral soil, especially in the deepest layers, suggesting that it should be considered, together with environmental variables when accounting GHG fluxes in degraded forests. Short-term effects of thinning on CO2 emissions were dependent on disturbance induced by logging operations and organic matter inputs. After thinning CH4 uptake increased significantly under selective treatment, independently from specific site-induced effects. N2O fluxes were characterized by low emissions in both sites and were not affected by treatments. Soil CO2 efflux was the largest component of global warming potential (GWP) from both sites (11,553 kg ha-1 y-1 on average). Although it has a large global warming potential, N2O contribution to GWP was about 131 kg CO2eq ha-1 y-1. The contribution of CH4-CO2 equivalent to total GWP showed a clear and significant CH4 sink behaviour under selective treatment (36 kg ha-1 y-1 on average). However, in the short-term both thinning approaches produced a weak effect on total GWP.

Continuous Spectrum LEDs Promote Seedling Quality Traits and Performance of Quercus ithaburensis var. macrolepis.

Regulation of the growth, development, and quality of plants by the control of light quality has attracted extensive attention worldwide. The aim of this study was to examine the effects of continuous LED spectrum for indoor plant pre-cultivation and to investigate the morphological and physiological responses of a common broadleaved tree species in Mediterranean environment, Quercus ithaburensis var. macrolepis at seedling developmental stage. Thus, the seedlings were pre-cultivated for 28 days, under five different LED light qualities: (1) Fluorescent (FL) as control light (2) L20AP67 (high in green and moderate in far-red), (3) AP673L (high in green and red), (4) G2 (highest in red and far-red), AP67 (high in blue, red, and far-red), and (5) NS1 (highest in blue and green and lowest in far-red) LEDs. Further examination was held at the nursery for 1 year, on several seedling quality traits. Indeed, AP67 and AP673L triggered higher leaf formation, while L20AP67 positively affected seedling shoot development. NS1 and AP67 LED pre-cultivated seedlings showed significantly higher root fibrosity than those of FL light. Furthermore, NS1 and AP673L LEDs induced fourfold increase on seedling root dry weight than FL light. Hence, evaluating the seedling nursery performance attributes, most of those photomorphogenetic responses previously obtained were still detectable. Even more so, LED pre-cultivated seedlings showed higher survival and faster growth indicating better adaptation even under natural light conditions, a fact further reinforced by the significantly higher Dickson's quality index acquired. In conclusion, the goal of each nursery management program is the production of high quality seedlings with those desirable traits, which in turn satisfy the specific needs for a particular reforestation site. Thus, the enhanced oak seedling quality traits formed under continuous LEDs spectrum especially of NS1 and AP673L pre-cultivation may potentially fulfill this goal.

EFFECTS OF COLD STRATIFICATION AND GA3 ON GERMINATION OF ARBUTUS UNEDO SEEDS OF THREE PROVENANCES.

BACKGROUND: Arbutus unedo is a valuable Mediterranean shrub as an ornamental plant as well as fruit tree. Fresh fruits of A. unedo are a good source of antioxidants, of vitamins C, E and carotenoids and also are characterized by the high content of mineral elements. MATERIALS AND METHODS: The effects of gibberellic acid (GA3) and cold stratification (CS) on seed germination performance were investigated in A. unedo seeds collected from three provenances in the Northern part of Greece. Seeds of each provenance were soaked in solutions of GA3 (500, 1000 or 2000 ppm) for 24 h and subsequently were subjected to CS at 3 - 5°C for 0, 1, 2, and 3 months. RESULTS: Non-stratified seeds of the three A. unedo provenances which were not treated with GA3 solutions exhibited very low germination. However, seed germination was significantly improved after a one-month period of CS. Similarly, the non-stratified seeds of all three provenances became non-dormant after the treatment with 2000 ppm GA3 and they germinated at high percentages. However, in untreated seeds with GA3, after a one-month CS period the seeds of the Pieria provenance exhibited higher germination percentage than that of Rodopi provenance seeds. Furthermore, in non-stratified seeds, the Pieria provenance seeds treated with GA3 germinated at higher percentages and more rapidly than those of the other two provenances. CONCLUSION: The results indicated that untreated seeds exhibited very low germination at 20/25°C. However, in all three provenances seed germinability was significantly improved by a one-month period of CS or treatment of seeds with 2000 ppm GA3. Furthermore, there was a considerable variability among seed provenances in response to the treatments which were applied.

Tree diversity affects chlorophyll a fluorescence and other leaf traits of tree species in a boreal forest.

An assemblage of tree species with different crown properties creates heterogeneous environments at the canopy level. Changes of functional leaf traits are expected, especially those related to light interception and photosynthesis. Chlorophyll a fluorescence (ChlF) properties in dark-adapted leaves, specific leaf area, leaf nitrogen content (N) and carbon isotope composition (δ13C) were measured on Picea abies (L.) H.Karst., Pinus sylvestris L. and Betula pendula Roth. in monospecific and mixed boreal forests in Europe, in order to test whether they were affected by stand species richness and composition. Photosynthetic efficiency, assessed by induced emission of leaf ChlF, was positively influenced in B. pendula by species richness, whereas P. abies showed higher photosynthetic efficiency in monospecific stands. Pinus sylvestris had different responses when it coexisted with P. abies or B. pendula. The presence of B. pendula, but not of P. abies, in the forest had a positive effect on the efficiency of photosynthetic electron transport and N in P. sylvestris needles, and the photosynthetic responses were positively correlated with an increase of leaf δ13C. These effects on P. sylvestris may be related to high light availability at the canopy level due to the less dense canopy of B. pendula. The different light requirements of coexisting species was the most important factor affecting the distribution of foliage in the canopy, driving the physiological responses of the mixed species. Future research directions claim to enhance the informative potential of the methods to analyse the responses of pure and mixed forests to environmental factors, including a broader set of plant species' functional traits and physiological responses.

Ecophysiology of seedlings of three Mediterranean pine species in contrasting light regimes.

Seasonal dynamics of net photosynthesis (Anet) in 2-year-old seedlings of Pinus brutia Ten., Pinus pinea L. and Pinus pinaster Ait. were investigated. Seedlings were grown in the field in two light regimes: sun (ambient light) and shade (25% of photosynthetically active radiation (PAR)). Repeated measures analyses over a 12-month period showed that Anet varied significantly among species and from season to season. Maximum Anet in sun-acclimated seedlings was low in winter (yet remained positive) and peaked during summer. Maximum Anet was observed in June in P. pinea (12 micromol m-2 s-1), July in P. pinaster (23 micromol m-2 s-1) and August in P. brutia (20 micromol m-2 s-1). Photosynthetic light response curves saturated at a PAR of 200-300 micromol m-2 s-1 in winter and in shade-acclimated seedlings in summer. Net photosynthesis in sun-acclimated seedlings did not saturate at PAR up to 1900 micromol m-2 s-1 in P. brutia and P. pinaster. Minimum air temperature of the preceding night was apparently one of the main factors controlling Anet during the day. In shade-acclimated seedlings, photosynthetic rates were reduced by 50% in P. brutia and P. pinaster and by 20% in P. pinea compared with those in sun-acclimated seedlings. Stomatal conductance was generally lower in shaded seedlings than in seedlings grown in the sun, except on days with a high vapor pressure deficit. Total chlorophyll concentration per unit leaf area, specific leaf area (SLA) and height significantly increased in P. pinea in response to shade, but not in P. pinaster or P. brutia. In response to shade, P. brutia showed a significant increase in total chlorophyll concentration but not SLA. Photosynthetic and growth data indicate that P. pinaster and P. brutia are more light-demanding than P. pinea.

Physiological responses of beech and sessile oak in a natural mixed stand during a dry summer.

Responses of CO2 assimilation and stomatal conductance to decreasing leaf water potential, and to environmental factors, were analysed in a mixed natural stand of sessile oak (Quercus petraea ssp. medwediewii) and beech (Fagus svlvatica L.) in Greece during the exceptionally dry summer of 1998. Seasonal courses of leaf water potential were similar for both species, whereas mean net photosynthesis and stomatal conductance were always higher in sessile oak than in beech. The relationship between net photosynthesis and stomatal conductance was strong for both species. Sessile oak had high rates of photosynthesis even under very low leaf water potentials and high air temperatures, whereas the photosynthetic rate of beech decreased at low water potentials. Diurnal patterns were similar in both species but sessile oak had higher rates of CO2 assimilation than beech. Our results indicate that sessile oak is more tolerant of drought than beech, due, in part, to its maintenance of photosynthesis at low water potential.