RESUMO
To determine G and P genotypes, we performed nested PCR on 307 rotavirus specimens collected in Dhaka, Bangladesh, during 2004-2005. G2 (43.3%) was detected at the highest frequency, followed by G4 (19.5%), G9 (13.7%), G1 (12.7%), and G3 (2.6%). P[8] was the most predominant genotype (53.2%), followed by P[4] (42.9%).
Assuntos
Gastroenterite/epidemiologia , Infecções por Rotavirus/epidemiologia , Rotavirus/classificação , Rotavirus/genética , Doença Aguda , Bangladesh/epidemiologia , Pré-Escolar , Fezes/virologia , Feminino , Gastroenterite/virologia , Genótipo , Humanos , Lactente , Masculino , Epidemiologia Molecular , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Prevalência , Rotavirus/isolamento & purificação , Infecções por Rotavirus/virologia , Análise de Sequência de DNARESUMO
BACKGROUND: Diarrhea, over the years, has killed millions of people and continues to be a major threat in Bangladesh. OBJECTIVES: To determine the incidence of norovirus infection in infants and young children with acute gastroenteritis in Dhaka City, Bangladesh and to determine the genogroup and genotype in norovirus-positive stool specimens. STUDY DESIGN: Fecal specimens were collected from infants and children with acute gastroenteritis in Dhaka City, Bangladesh from October 2004 to September 2005, and examined for norovirus by reverse transcription-polymerase chain reaction. RESULTS: Noroviruses were detected in 41 of 917 fecal specimens. Molecular analysis of norovirus was carried out by sequencing methods. Only norovirus GII/4 strains were detected during this study. The dominant genotype throughout the study period was GII/4. Norovirus infections were most commonly observed in winter and rainy seasons in Dhaka City. The common clinical symptoms in norovirus-infected patients were diarrhea (90%), vomiting (75%) and abdominal pain (46%). CONCLUSIONS: This is the first epidemiological research of norovirus in Bangladesh. Norovirus is an important enteropathogen responsible for viral gastroenteritis among infants and children in Bangladesh.
Assuntos
Infecções por Caliciviridae , Gastroenterite , Norovirus/isolamento & purificação , Bangladesh/epidemiologia , Infecções por Caliciviridae/epidemiologia , Infecções por Caliciviridae/virologia , Criança , Fezes/virologia , Gastroenterite/epidemiologia , Gastroenterite/virologia , Genótipo , Humanos , Lactente , Epidemiologia Molecular , Dados de Sequência Molecular , Norovirus/classificação , Norovirus/genética , Filogenia , RNA Viral/análise , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Estações do Ano , Análise de Sequência de DNARESUMO
Between 2004 and 2005, 917 fecal specimens were collected from children below age 5 who presented to Child Health Institute for treatment of diarrhoea in Dhaka City, Bangladesh. The specimens were screened by RT-PCR for the presence of group A rotavirus and positive stools genotyped. Group A rotavirus was detected in 307 stools and serotype G3P[8] strains were detected in nine specimens. Sequence analysis clustered the G3 strains into one distinct lineage (lineage I) with other Asian G3 strains. In addition, one amino acid change at position 96 in antigenic region A, similar to lineage II G3 Chinese strains, was noted. To our knowledge this is the first report of serotype G3 strains in Bangladesh since 1993 and the first report of the molecular characterization of these strains.