Development of a Frugal, In Situ Sensor Implementing a Ratiometric Method for Continuous Monitoring of Turbidity in Natural Waters.

Turbidity is a commonly used indicator of water quality in continental and marine waters and is mostly caused by suspended and colloidal particles such as organic and inorganic particles. Many methods are available for the measurement of turbidity, ranging from the Secchi disk to infrared light-based benchtop or in situ turbidimeters as well as acoustic methods. The operational methodologies of the large majority of turbidity instruments involve the physics of light scattering and absorption by suspended particles when light is passed through a sample. As such, in the case of in situ monitoring in water bodies, the measurement of turbidity is highly influenced by external light and biofouling. Our motivation for this project is to propose an open-source, low-cost in situ turbidity sensor with a suitable sensitivity and operating range to operate in low-to-medium-turbidity natural waters. This prototype device combines two angular photodetectors and two infrared light sources with different positions, resulting in two different types of light detection, namely nephelometric (i.e., scattering) and attenuation light, according to the ISO 7027 method. The mechanical design involves 3D-printed parts by stereolithography, which are compatible with commercially available waterproof enclosures, thus ensuring easy integration for future users. An effort was made to rely on mostly off-the-shelf electronic components to encourage replication of the system, with the use of a highly integrated photometric front-end commonly used in portable photoplethysmography systems. The sensor was tested in laboratory conditions against a commercial benchtop turbidimeter with Formazin standards. The monitoring results were analyzed, obtaining a linear trendline from 0 to 50 Nephelometric Turbidity Unit (NTU) and an accuracy of +/-0.4 NTU in the 0 to 10 NTU range with a response time of less than 100 ms.

Ion chromatograph with three-dimensional printed absorbance detector for indirect ultraviolet absorbance detection of phosphate in effluent and natural waters.

An ion chromatography system employing a low-cost three-dimensional printed absorbance detector for indirect ultraviolet detection towards portable phosphate analysis of environmental and industrial waters has been developed. The optical detection cell was fabricated using stereolithography three-dimensional printing of nanocomposite material. Chromatographic analysis and detection of phosphate were carried out using a CS5A 4 × 250 mm analytical column with indirect ultraviolet detection using a 255 nm light-emitting diode. Isocratic elution using a 0.6 mM potassium phthalate eluent combined with 1.44 mM sodium bicarbonate was employed at a flow rate of 0.75 mL/min. A linear calibration range of 0.5 to 30 mg/L PO4 3- applicable to environmental and wastewater analysis was achieved. For retention time and peak area repeatability, relative standard deviation values were 0.68 and 4.09%, respectively. Environmental and wastewater samples were analyzed with the optimized ion chromatography platform and the results were compared to values obtained by an accredited ion chromatograph. For the analysis of environmental samples, relative errors of <14 % were achieved. Recovery analysis was also carried out on both freshwater and wastewater samples and recovery results were within the acceptable range for water analysis using standard ion chromatography methods.

Mobile Acoustic Wave Platform Deployment in the Amazon River: Impact of the Water Sample on the Love Wave Sensor Response.

This paper presents an experimental platform allowing in situ measurement in an aqueous medium using an acoustic Love wave sensor. The aim of this platform, which includes the sensor, a test cell for electrical connections, a microfluidic chip, and a readout electronic circuit, is to realize a first estimation of water quality without transportation of water samples from the field to the laboratory as a medium-term objective. In the first step, to validate the ability of such a platform to operate in the field and in Amazonian water, an isolated and difficult-to-access location, namely, the floodplain Logo Do Curuaï in the Brazilian Amazon, was chosen. The ability of such a platform to be transported, installed on site, and used is discussed in terms of user friendliness and versatility. The response of the Love wave sensor to in situ water samples is estimated according to the physical parameters of Amazonian water. Finally, the very good quality of the acoustic response is established, potential further improvements are discussed, and the paper is concluded.

Love Acoustic Wave-Based Devices and Molecularly-Imprinted Polymers as Versatile Sensors for Electronic Nose or Tongue for Cancer Monitoring.

Cancer is a leading cause of death worldwide and actual analytical techniques are restrictive in detecting it. Thus, there is still a challenge, as well as a need, for the development of quantitative non-invasive tools for the diagnosis of cancers and the follow-up care of patients. We introduce first the overall interest of electronic nose or tongue for such application of microsensors arrays with data processing in complex media, either gas (e.g., Volatile Organic Compounds or VOCs as biomarkers in breath) or liquid (e.g., modified nucleosides as urinary biomarkers). Then this is illustrated with a versatile acoustic wave transducer, functionalized with molecularly-imprinted polymers (MIP) synthesized for adenosine-5'-monophosphate (AMP) as a model for nucleosides. The device including the thin film coating is described, then static measurements with scanning electron microscopy (SEM) and electrical characterization after each step of the sensitive MIP process (deposit, removal of AMP template, capture of AMP target) demonstrate the thin film functionality. Dynamic measurements with a microfluidic setup and four targets are presented afterwards. They show a sensitivity of 5 Hz·ppm(-1) of the non-optimized microsensor for AMP detection, with a specificity of three times compared to PMPA, and almost nil sensitivity to 3'AMP and CMP, in accordance with previously published results on bulk MIP.

Functionality integration in stereolithography 3D printed microfluidics using a "print-pause-print" strategy.

Stereolithography 3D printing, although an increasingly used fabrication method for microfluidic chips, has the main disadvantage of producing monolithic chips in a single material. We propose to incorporate during printing various objects using a "print-pause-print" strategy. Here, we demonstrate that this novel approach can be used to incorporate glass slides, hydrosoluble films, paper pads, steel balls, elastic or nanoporous membranes and silicon-based microdevices, in order to add microfluidic functionalities as diverse as valves, fluidic diodes, shallow chambers, imaging windows for bacteria tracking, storage of reagents, blue energy harvesting or filters for cell capture and culture.

Quantitative full-colour transmitted light microscopy and dyes for concentration mapping and measurement of diffusion coefficients in microfluidic architectures.

A simple and versatile methodology has been developed for the simultaneous measurement of multiple concentration profiles of colourants in transparent microfluidic systems, using a conventional transmitted light microscope, a digital colour (RGB) camera and numerical image processing combined with multicomponent analysis. Rigorous application of the Beer-Lambert law would require monochromatic probe conditions, but in spite of the broad spectral bandwidths of the three colour channels of the camera, a linear relation between the measured optical density and dye concentration is established under certain conditions. An optimised collection of dye solutions for the quantitative optical microscopic characterisation of microfluidic devices is proposed. Using the methodology for optical concentration measurement we then implement and validate a simplified and robust method for the microfluidic measurement of diffusion coefficients using an H-filter architecture. It consists of measuring the ratio of the concentrations of the two output channels of the H-filter. It enables facile determination of the diffusion coefficient, even for non-fluorescent molecules and nanoparticles, and is compatible with non-optical detection of the analyte.

On-chip multi-electrochemical sensor array platform for simultaneous screening of nitric oxide and peroxynitrite.

In this work we report on the design, microfabrication and analytical performances of a new electrochemical sensor array (ESA) which allows for the first time the simultaneous amperometric detection of nitric oxide (NO) and peroxynitrite (ONOO(-)), two biologically relevant molecules. The on-chip device includes individually addressable sets of gold ultramicroelectrodes (UMEs) of 50 µm diameter, Ag/AgCl reference electrode and gold counter electrode. The electrodes are separated into two groups; each has one reference electrode, one counter electrode and 110 UMEs specifically tailored to detect a specific analyte. The ESA is incorporated on a custom interface with a cell culture well and spring contact pins that can be easily interconnected to an external multichannel potentiostat. Each UME of the network dedicated to the detection of NO is electrochemically modified by electrodepositing thin layers of poly(eugenol) and poly(phenol). The detection of NO is performed amperometrically at 0.8 V vs. Ag/AgCl in phosphate buffer solution (PBS, pH = 7.4) and other buffers adapted to biological cell culture, using a NO-donor. The network of UMEs dedicated to the detection of ONOO(-) is used without further chemical modification of the surface and the uncoated gold electrodes operate at -0.1 V vs. Ag/AgCl to detect the reduction of ONOOH in PBS. The selectivity issue of both sensors against major biologically relevant interfering analytes is examined. Simultaneous detection of NO and ONOO(-) in PBS is also achieved.