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1.
BMC Health Serv Res ; 24(1): 267, 2024 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-38431588

RESUMO

BACKGROUND: The COVID-19 pandemic has been noted to decrease access to maternal health and family planning services globally. However, evidence from the Middle East and North Africa region is very scarce and limited. We qualitatively explored women's experiences in accessing maternal health and family planning services during the COVID-19 lockdown months in the two Egyptian governorates of Port Said and Souhag. METHODS: Using a case study design, semi-structured phone interviews were conducted with a total of 40 women aged 18-35 years from Port Said and Souhag governorates in Egypt. Interviews explored women's experiences in accessing maternal health and family planning services during COVID-19 lockdown months, their coping strategies, and impact of challenges and/or coping strategies on participants and their families. The collected data was analyzed manually using qualitative thematic analysis. RESULTS: Many participants were unable to access maternal health and family planning services during COVID-19 lockdown due to fear of contracting the virus, closure of health facilities, changing service hours, family planning method or drug stock-outs, and/or financial constraints. The above challenges in accessing services along with coping strategies that some women and their families used exposed women to additional health risks, including unintended pregnancies, and posed several social, emotional, and financial burdens to many. CONCLUSIONS: The COVID-19 pandemic and associated lockdown measures undermined women's access to maternal and family planning services and interfered with their ability to achieve their reproductive goals. The paper concludes with a number of recommendations to ensure access to maternal and family planning services at times of crisis. Those recommendations include: (1) adapting reliable guidelines from humanitarian settings, (2) providing adequate guidance to healthcare providers and the public to tackle fears and misinformation, (3) making self-care products available such as oral contraceptive pills, vaginal rings and self- administered injectables, (4) involving other health professionals in the provision of maternal and family planning services through task-sharing/shifting, (5) expanding the use of telemedicine and/or digital health services especially to those living in remote areas and (6) raising policymakers' awareness of the centrality of reproductive rights and the importance of protecting them at all times.


Assuntos
COVID-19 , Serviços de Saúde Materna , Gravidez , Humanos , Feminino , Serviços de Planejamento Familiar , Egito/epidemiologia , Saúde Materna , Pandemias , COVID-19/epidemiologia , Controle de Doenças Transmissíveis
2.
Methods Mol Biol ; 2191: 201-220, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32865747

RESUMO

Zebrafish are an excellent model organism to study many aspects of vertebrate sensory encoding and behavior. Their escape responses begin with a C-shaped body bend followed by several swimming bouts away from the potentially threatening stimulus. This highly stereotyped motor behavior provides a model for studying startle reflexes and the neural circuitry underlying multisensory encoding and locomotion. Channelrhodopsin (ChR2) can be expressed in the lateral line and ear hair cells of zebrafish and can be excited in vivo to elicit these rapid forms of escape. Here we review our methods for studying transgenic ChR2-expressing zebrafish larvae, including screening for positive expression of ChR2 and recording field potentials and high-speed videos of optically evoked escape responses. We also highlight important features of the acquired data and provide a brief review of other zebrafish research that utilizes or has the potential to benefit from ChR2 and optogenetics.


Assuntos
Channelrhodopsins/genética , Potenciais Evocados/genética , Neurônios/metabolismo , Optogenética/métodos , Animais , Animais Geneticamente Modificados/genética , Channelrhodopsins/fisiologia , Potenciais Evocados/fisiologia , Células Ciliadas Auditivas/metabolismo , Larva/fisiologia , Locomoção/genética , Locomoção/fisiologia , Neurônios/patologia , Reflexo de Sobressalto/fisiologia , Natação/fisiologia , Peixe-Zebra/genética , Peixe-Zebra/fisiologia
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