Rice seed (IR64) priming with potassium humate for improvement of seed germination, seedling growth and antioxidant defense system under arsenic stress.

The intolerable levels of arsenic (As) in groundwater and its application in rice cultivation are continuously affecting the rice production in Ganga-Meghna-Brahmaputra (GMB) plain. The reduced germination and plant growth rates under excessive As stress ultimately lead to lower yield. To mitigate this concerning issue, the present study was carried out to evaluate the potential of K-humate priming on seed germination and plant growth under As stress. Seeds were primed with 100 mg/l K-humate for 12 h prior to germination. The germination percentages in unprimed seeds were 65 ±â€¯5.0% and 58.3 ±â€¯7.6% under stress level of 50 µM AsV and 50 µM AsIII, respectively. However, germination percentage in K-humate primed seeds were 75 ±â€¯5.0% and 68.3 ±â€¯2.9% under AsV and AsIII stress, respectively. The vigour index I (VG I) and vigour index II (VG II) recorded on 12 DAS (days after seeding) were also increased by 1.47 and 1.51 fold, respectively with K-humate supplementation under As stress. Detrimental effects of AsIII on seed germination, seedling growth and other physiological parameters were more suppressive than AsV. Application of K-humate not only improved seed germination, seedling growth and nutrient uptake but also decreased the oxidative stress markers and antioxidant activities by minimizing As uptake and translocation in the seedlings.

Entrapment of atmospheric particle bound heavy metals by ferns as evidenced by lead (Pb) isotope and MixSIAR: Implications for improving air quality.

Plant metal uptake can occur through both soil-root and atmospheric transfer from leaves. The latter holds potential implications for development of biofiltration systems. To explore this potential, it is crucial to understand entrapment capacity and metal sources within plants. As ferns absorb materials from atmosphere, this study focuses on two abundant fern species growing in densely populated and highly polluted regions of Eastern India. Gravimetric quantification, elemental concentration and Pb isotopic analyses were performed by segregating the ferns into distinct components: foliage dusts (loose dust (LD) and wax-bound dust (WD)) and plant tissue (leaves and roots). To understand metal sources, the study analyzes soil, and atmospheric particulates (PM10 and dust fall (DF)). Results indicate that, while LDs have soil dust influence, wax entraps atmospheric particulates and translocates them inside the leaves. Furthermore, roots demonstrate dissimilar isotopic ratios from soil, while displaying close association with atmospheric particulates. Isotopic composition and subsequent mixing model reveal dominant contribution from DF in leaves (53-73%) and roots (33-86%). Apart from DF, leaf Pb is sourced from PM10 (21-38%) with minimal contribution from soil (6-10%). Conversely, in addition to dominance from DF, roots source Pb primarily from soil (12-62%) with a meagre 2-8% contribution from PM10.

Seasonal variation of atmospheric Pb sources in Singapore - Elemental and lead isotopic compositions of PM10 as source tracer.

Southeast Asia has become a hotspot of anthropogenic particulate matter (PM) emissions due to increased coal combustion, high-temperature industrial operations, vehicular traffic, and agricultural biomass burning. Lead (Pb), a criteria pollutant, bound to such PM can be hazardous when inhaled, even at extremely low concentrations. Precise and accurate source apportionment of atmospheric Pb is thus, critical in order to minimize its exposure. This study investigates the sources of atmospheric Pb in Singapore aerosol samples (PM10) using Pb isotopes and elemental composition as tracers of contamination sources. PM10 aerosol sampling was conducted over a period of 1 year from June 2017 to May 2018 to capture the seasonal variations in sources of atmospheric Pb. Elemental concentrations reveal particularly high enrichment factors for Pb, Cu, V, Ni and Zn, especially when under the influence of southwest (SW) and inter monsoon (IM) winds. Pb isotopic ratios across the three seasons (206/207Pb = 1.147-1.150 and 208/207Pb = 2.420-2.428) are not significantly different. The Pb isotopic signatures and V/Ni ratios for all three seasons overlap with those of gasoline, diesel and ship emissions. Moreover, V/Pb values of more than unity for SW and IM winds suggest influence of transboundary coal combustion emissions particularly from Indonesia. Consequently, using Pb isotopic fingerprints and elemental ratios, we find that the primary sources of atmospheric Pb are vehicular & ship emissions, heavy oil combustion, transboundary coal combustion emissions, waste incineration and recirculation of historic leaded gasoline.

Effect of sulfate application on inhibition of arsenic bioaccumulation in rice (Oryza sativa L.) with consequent health risk assessment of cooked rice arsenic on human: A pot to plate study.

Arsenic (As) in rice is posing a serious threat worldwide and consumption of As contaminated rice by human is causing health risks. A pot experiment with different levels of sulfate dosage (0, 20, 40, 60 and 80 mg/kg) was set up in this study to explore the influence of sulfate fertilizer on rice plant growth, yield, and As accumulation in rice grain. Apart from As bioaccumulation in rice grains, the As fraction of cooked rice was quantified, and the health risks associated with cooked rice consumption were also investigated. The sulfate application significantly (p ≤ 0.05) enhanced the chlorophyll, tiller number, grains per panicle, grain and biomass yield under As stressed condition. The sulfate application also reduced the oxidative stress and antioxidant activity in rice plants. Sulfate fertigation improved the accumulation of total sulfur (S) and reduced the uptake and translocation of As in rice plants. Arsenic concentration in rice grain was reduced by 50.1% in S80 treatment (80 mg of sulfate/kg of soil) as compared to S0 set. The reduction percentage of As in cooked parboiled and sunned rice with correspond to raw rice ranged from 55.9 to 74% and 40.3-60.7%, respectively. However, the sulfate application and cooking of parboiled rice reduced the potential non-cancer and cancer risk as compared to sunned rice. The S80 treatment and cooking of parboiled rice reduce the As exposure for both children and adults by 51% as compared to cooked sunned rice under S80 treatment and this trend was similar for all treatments. Therefore, sulfate application in soil can be recommended to produce safer rice grains and subsequent cooking of parboiled rice grain with low-As contaminated water need to be done to avoid any potential health risk in As endemic areas.

Application of potassium humate to reduce arsenic bioavailability and toxicity in rice plants (Oryza sativa L.) during its course of germination and seedling growth.

Arsenic (As), a metalloid is a class I carcinogen and is a major problem in various parts of the world. Food crops are severely affected due to As poisoning and suffer from low germination, yield and disfiguration of morphological and anatomical traits. To attenuate such adverse effects and tone down As uptake by plants, the present study attempts to explore the role of K-humate (KH) in alleviation of As toxicity in rice. KH was administered in the growth media containing 800 ppb As (III) at varying doses to observe the stress alleviating capacity of the amendment. Five treatments were investigated, viz: (a) 800 ppb As (control), (b) 800 ppb As + 25 ppm KH, (c) 800 ppb As + 50 ppm KH, (d) 800 ppb As + 75 ppm KH and (e) 800 ppb As + 100 ppm KH. The results of the amendment administration were noted at 14 days after seeding (DAS). Application of KH significantly improved germination percentage, vigour indices and chlorophyll content by reducing the oxidative stress, antioxidant and antioxidant enzyme activities under As stress. In vivo detection of reactive oxygen species (ROS) using DCF-2DA fluorescent dye and scanning electron microscope (SEM) study of root further depicted that KH application effectively reduced ROS formation and improved root anatomical structure under As stress, respectively. Gradually increasing concentrations of KH was capable of decreasing the bioavailability of As to the rice plants, thus minimizing toxic effect of the metalloid.