RESUMO
In contrast to the normal thyroid hormone receptor, the v-erbA product fails to bind hormone due to mutations in the C-terminal ligand binding domain and thus appears to represent a hormone-independent, oncogenic transcription factor. Therefore, we asked whether or not the C-terminal domain of v-erbA is required for its biological activity and putative transcriptional control functions by analysing mutants with altered C-termini. A v-erbA protein truncated in the C-terminal domain lacked detectable biological activity in transformed erythroblasts and its transcriptional repression function with respect to the band 3 gene was abolished. The protein displayed a nuclear location and could still bind to DNA, indicating that the N-terminal region retained DNA-binding activity but was insufficient to produce characteristic v-erbA changes in erythroblasts. Another biologically defective v-erbA variant with a small frameshift towards the extreme C-terminus also failed to repress band 3, indicating a requirement for a specific C-terminal structure in repression. However, this mutant retained partial biological activity, stimulating erythroblasts to grow at a higher rate than cells containing a completely inactive, deleted v-erbA gene. The results demonstrate that the mutated hormone-binding domain, in addition to the DNA-binding region, is critical for v-erbA biological and transcriptional control functions.
Assuntos
Transformação Celular Neoplásica , Genes Reguladores , Oncogenes , Proteínas Oncogênicas de Retroviridae/genética , Transcrição Gênica , Sequência de Aminoácidos , Animais , Sequência de Bases , Northern Blotting , Divisão Celular , Embrião de Galinha , Códon/genética , Fibroblastos/citologia , Variação Genética , Dados de Sequência Molecular , Mutação , Sondas de Oligonucleotídeos , Proteínas Oncogênicas v-erbA , Plasmídeos , Proteínas Tirosina Quinases/genética , Mapeamento por Restrição , TransfecçãoRESUMO
We have compared the effects of the v-erb A oncogene on proliferation and differentiation of normal erythroid progenitors with those of tyrosine kinase oncogenes, e.g. v-sea. For this, a v-erb A retrovirus containing the neomycin resistance gene as a selectable marker or, alternatively, a v-erb A-ts v-sea retrovirus were used to infect normal bone marrow cells. V-erb A induced the outgrowth of immature, erythropoietin(EPO)-dependent erythroid cells from infected bone marrow which ceased to proliferate and disintegrated after 9 to 18 divisions. In contrast, ts-v-sea erythroblasts grew for the expected 25 to 40 population doublings in the absence of EPO. Transcription of the erythrocyte genes carbonic anhydrase II and erythrocyte anion transporter was significantly inhibited in v-erb A infected erythroblasts, indicating that v-erb A alone was sufficient for the repression of the erythrocyte-specific genes observed in AEV-transformed leukemic cells. A detailed analysis of the differentiation phenotype induced by v-erb A in erythroblasts (in the presence or absence of a temperature-inactivated ts sea oncogene) indicates that v-erb A-erythroblasts express a partially mature, aberrant phenotype characterized by the coexpression of mature and immature differentiation antigens. This phenotype clearly differs from that induced by tyrosine kinase oncogenes in erythroid cells.
Assuntos
Anidrases Carbônicas/genética , Transformação Celular Neoplásica , Eritroblastos/citologia , Eritrócitos/citologia , Células-Tronco Hematopoéticas/citologia , Oncogenes , Proteínas Oncogênicas de Retroviridae/genética , Transcrição Gênica , Animais , Antígenos de Superfície , Diferenciação Celular , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Embrião de Galinha , Galinhas , Eritroblastos/efeitos dos fármacos , Eritrócitos/metabolismo , Eritropoetina/farmacologia , Fibroblastos , Células-Tronco Hematopoéticas/metabolismo , Proteínas Oncogênicas v-erbA , Fatores de Transcrição/genéticaRESUMO
Glial cell line-derived neurotrophic factor (GDNF) mediates neuronal survival through a receptor complex composed of the c-Retproto-oncogene and GFR alpha-1, a member of a family of GPI-anchored receptors. The extent of cross-talk between GDNF and GFR alpha receptors and its possible significance for c-Ret activation is presently unclear. Using chemical crosslinking we demonstrate here a specific interaction between GDNF and GFR alpha-2 expressed in COS cells, albeit of a lower affinity than the one between GDNF and GFR alpha-1. In addition, GFR alpha-2 mediated crosslinking of GDNF of c-Ret as well as ligand-dependent stimulation of c-Ret tyrosine phosphorylation. We also describe the isolation of a novel, more divergent member of the GFR alpha family, GFR alpha-3, which did not bind GDNF directly, but was able to mediate crosslinking of GDNF to c-Ret when both receptors were coexpressed in COS cells. Thus, all three GFR alpha receptors mediate GDNF binding to c-Ret with efficiencies GFR alpha-1 > GFR alpha-2 > GFR alpha-3. c-Ret showed high levels of constitutive tyrosine autophosphorylation upon overexpression in COS cells, which was inhibited in a dose-dependent manner by coexpression with any of the GFR alpha receptors, suggesting that GFR alpha s may also provide a gain control mechanism to increase the signal-to-noise ratio of the response to ligand. GFR alpha-2 showed a dynamic pattern of expression in rat brain, distinct from that of GFR alpha-1, characterized by high expression in cortex, basal forebrain, and specific layers of the olfactory bulb, and low or no expression in substantia nigra, cerebellum, and motor nuclei. GFR alpha-2, but not GFR alpha-3 mRNA expression was highly induced in several nuclei after stimulation with kainic acid. In contrast to GFR alpha-1 and GFR alpha-2, GFR alpha-3 expression in postnatal and adult brain was highly restricted. Developmentally regulated expression of GFR alpha-3 was, however, detected in several peripheral organs and ganglia. Together, these results indicate complementary roles for GFR alpha receptors in the regulation of c-Ret activity and the maintenance of distinct neuronal circuits in the central and peripheral nervous systems.
Assuntos
Proteínas de Drosophila , Glicosilfosfatidilinositóis/metabolismo , Glicoproteínas de Membrana , Fatores de Crescimento Neural , Proteínas do Tecido Nervoso/fisiologia , Proteínas Proto-Oncogênicas/fisiologia , Receptores Proteína Tirosina Quinases/fisiologia , Receptores de Fator de Crescimento Neural , Sequência de Aminoácidos , Animais , Animais Recém-Nascidos , Encéfalo/metabolismo , Células COS , Clonagem Molecular , Ativação Enzimática , Feto , Regulação da Expressão Gênica no Desenvolvimento/genética , Fator Neurotrófico Derivado de Linhagem de Célula Glial , Receptores de Fator Neurotrófico Derivado de Linhagem de Célula Glial , Humanos , Ligantes , Masculino , Dados de Sequência Molecular , Proteínas do Tecido Nervoso/metabolismo , Fosforilação , Proteínas Proto-Oncogênicas/biossíntese , Proteínas Proto-Oncogênicas/genética , Proteínas Proto-Oncogênicas c-ret , Ratos , Ratos Wistar , Receptores Proteína Tirosina Quinases/biossíntese , Receptores Proteína Tirosina Quinases/genética , Receptores de Superfície Celular/biossíntese , Receptores de Superfície Celular/genética , Tirosina/metabolismoRESUMO
Major histocompatibility complex (MHC) class I molecules are trimolecular complexes consisting of a heavy chain (HC), beta2-microglobulin (beta2m), and a short peptide. Assembly of MHC class I molecules is thought to take place early during biosynthesis. Deficiency in either beta2m or the transporter associated with antigen processing (TAP) results in accumulation of class I molecules in the endoplasmic reticulum (ER). In this study, we have assessed peptide binding to TAP and MHC class I in purified microsomes derived from wild-type, TAP1(-/-), beta2m-/-, and TAP1/beta2m-/- mice using a cross-linkable H-2Kb-binding peptide. This enabled us to study the influence of an intact TAP complex and beta2m on peptide binding to MHC class I and to analyze the stepwise interaction of peptide with TAP and MHC class I molecules. Peptide bound both immature and mature (terminally glycosylated) class I molecules in intact as well as permeabilized microsomes from wild-type mice. Efficient peptide binding to immature class I molecules was also detected in permeabilized microsomes from TAP1(-/-) mice. In contrast, no peptide binding to beta2m-free HC was detected in permeabilized microsomes from beta2m-/- and TAP1/beta2m-/- mice. However, the addition of exogenous beta2m allowed peptide binding to class I in permeabilized beta2m-/- and TAP1/beta2m-/- microsomes. These results demonstrate that a preformed class I HC middle dotbeta2m heterodimer is necessary for efficient peptide binding under physiological conditions. The observed peptide binding to class I in permeabilized TAP1(-/-) microsomes further suggests that TAP1 is not required for peptide binding to class I in the ER. Finally, kinetic studies allowed the demonstration of a stepwise binding of peptide to TAP, subsequent translocation across the ER membrane, a step that required ATP hydrolysis, and binding of peptide to preformed class I HC.beta2m heterodimers.
Assuntos
Proteínas de Transporte/metabolismo , Antígenos H-2/metabolismo , Antígenos de Histocompatibilidade Classe I/metabolismo , Microssomos Hepáticos/metabolismo , Microglobulina beta-2/genética , Trifosfato de Adenosina/metabolismo , Sequência de Aminoácidos , Animais , Ligação Competitiva , Proteínas de Transporte/genética , Glicosilação , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Mutantes , Dados de Sequência Molecular , Ovalbumina/metabolismo , Ligação ProteicaRESUMO
We have characterized a growth factor-inducible gene, pghs-B, isolated by differential screening of a lambda complementary DNA library from RNA of serum-stimulated NIH 3T3 cells which encodes a 604-amino acid protein presenting high similarity with prostaglandin G/H synthase (PGHS). Induction of both pghs-B mRNA and protein is rapid and remains at high levels for several hours. The increase in pghs-B mRNA is mainly due to transcriptional activation of the gene. PGHS-B has been expressed in the baculovirus system and has been demonstrated to be involved in prostaglandin synthesis and to be inhibited by indomethacin, demonstrating that PGHS-B is indeed an enzyme related to PGHS. The pghs-B gene maps to the [1G-1H2] region of the murine genome.
Assuntos
Genes , Prostaglandina-Endoperóxido Sintases/genética , Células 3T3/efeitos dos fármacos , Células 3T3/enzimologia , Sequência de Aminoácidos , Animais , Baculoviridae/genética , Sequência de Bases , Mapeamento Cromossômico , Ciclo-Oxigenase 2 , DNA/genética , Indução Enzimática , Vetores Genéticos , Masculino , Camundongos , Camundongos Endogâmicos BALB C/genética , Camundongos Mutantes/genética , Dados de Sequência Molecular , Prostaglandina-Endoperóxido Sintases/biossíntese , Proteínas Recombinantes de Fusão/biossíntese , Transcrição GênicaRESUMO
We have established Rat-1A cell lines constitutively expressing c-Fos and the two products of the fosB gene, FosB and its short form, FosB/SF. The expressed proteins in the different stable transfectants have been characterized by immunofluorescence and immunoprecipitation analysis. Our results demonstrate that constitutive expression of FosB, like the constitutive expression of c-Fos and, to a lesser extent, FosB/SF, results in cells that grow to increased saturation densities and have the ability to grow in an anchorage-independent manner. Most important is the finding that expression of these proteins augments the tumorigenic potential of Rat-1A cells. These results show that both forms of FosB have a stimulatory effect on cell proliferation.