1.
Nat Methods
; 6(9): 639-41, 2009 Sep.
Artigo
em Inglês
| MEDLINE
| ID: mdl-19668203
RESUMO
We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.