Analytical target-agnostic piston sensing for segmented telescopes using sparse redundant baseline pairs.

Piston correction is the key to achieving high resolution of segmented telescopes. Phasing with extended objects is still challenging. In this Letter, we propose an analytical target-agnostic phasing approach using redundant baseline pairs. It is derived that the mixed phase distribution caused by redundant sampling can be decoupled via phase modulation. Then the pistons can be resolved by performing phase cross-correlation to remove the object phase. We validate this theory through simulations and experiments. It does not require additional optical paths and is relatively robust against noise, thus providing a simple, fast, and low-system-complexity solution for piston monitoring of the segmented telescope over the period of imaging complex scenes.

Weak non-line-of-sight target echoes extraction without accumulation.

Non-line-of-sight (NLOS) technology has been rapidly developed in recent years, allowing us to visualize or localize hidden objects by analyzing the returned photons, which is expected to be applied to autonomous driving, field rescue, etc. Due to the laser attenuation and multiple reflections, it is inevitable for future applications to separate the returned extremely weak signal from noise. However, current methods find signals by direct accumulation, causing noise to be accumulated simultaneously and inability of extracting weak targets. Herein, we explore two denoising methods without accumulation to detect the weak target echoes, relying on the temporal correlation feature. In one aspect, we propose a dual-detector method based on software operations to improve the detection ability for weak signals. In the other aspect, we introduce the pipeline method for NLOS target tracking in sequential histograms. Ultimately, we experimentally demonstrated these two methods and extracted the motion trajectory of the hidden object. The results may be useful for practical applications in the future.

Stray light control for the space-agile optical system with a pointing mirror.

The space-agile optical composite detection (SOCD) system with a pointing mirror possesses flexible and fast response ability. Like other space telescopes, if the stray light is not properly eliminated, it may result in a false response or noise that floods the real light signal due to the low illuminance and large dynamic range of the target. The paper shows the optical structure layout, the decomposition of the optical processing index and roughness control index, the stray light suppression requirements, and the detailed stray light analysis process. The pointing mirror and ultra-long afocal optical path increase the difficulty of stray light suppression in the SOCD system. This paper presents the design method of a special-shaped aperture diaphragm and entrance baffle, black baffle surface testing, simulating, selection, and stray light suppression analysis process. The special-shaped entrance baffle has a significant effect on the suppression of stray light and reduced dependence on the platform posture of the SOCD system.

Robust Risley prism control based on disturbance observer for system line-of-sight stabilization.

In this paper, a robust control based on disturbance observer is proposed to improve the tracking accuracy of the Risley prism system (RPS). Applying the flexible thin-wall ring mechanism in the RPS causes a series of tracking and pointing challenges. Disturbances such as friction, shaft deformation, and model perturbation significantly deteriorate the tracking and pointing accuracy of the RPS. Two different observer-based control methods are proposed to guarantee the tracking precision of the RPS. Moreover, the disturbance observation and compensation (DOC) performance of the proposed methods is analyzed and compared. Finally, simulation and experiment results indicate that the proposed control methods, especially the DOC-expanded state observer control mode, obtain the best performance for disturbance rejection in the RPS.

Optical design and stray light control for a space-based laser space debris removal mission.

In low-Earth orbit, the already existing population of small and medium debris (between 1 cm and several dozens of cm) is a concrete threat to operational satellites. A space-based laser space debris removal (SLDR) system that can remove hazardous debris around selected space assets appears to be a flexible and effective project. To achieve high-precision tracking and emitting, the optical system of the SLDR mission includes a target-detection telescope and emitting telescope, adopting a common light path structure. The optical design results, system performance, tolerance budget, and detailed stray light control design are presented in this paper. The large-aperture off-axis two-mirror beam-narrowing system characteristics are also discussed in terms of stray light control. This paper will present the lateral-displacement (LD) setting, two-stage fore baffle design, black baffle surface selection, and opening direction of the telescope door. The results showed that the stray light elimination reaches a 10-9 order, meeting design requirements.

Disturbance compensation of a multiaperture imaging system based on a coupling rotating prism using an improved model compensation control.

In this paper, a cascade double-loop control (DLC) combined with modeling compensation methods is proposed to improve the tracking precision of the multiaperture imaging system (MAIS). The application of the flexible thin-wall ring mechanism in the coupling rotating prism (CRP) system causes a series of tracking and pointing challenges. Disturbances such as friction, shaft deformation, and model perturbation significantly deteriorate the tracking and pointing accuracy of the CRP. Two different modeling compensation methods that are interfaced with classical DLC are proposed to guarantee the tracking precision of the MAIS. Moreover, the disturbance observation and compensation performance of two different modeling compensation methods are analyzed and compared. Finally, simulation and experiment results indicate that the proposed control methods, especially model compensation based on speed close-loop control, obtain the best performance for disturbance rejection in the MAIS.

Closed-loop laser illumination algorithm using return photon counts.

Boresight and jitter are two fundamental pointing errors of laser illumination systems. A triangular-scanning algorithm is proposed to estimate the direction of the boresight via a three-step maximum boresight estimation and laser beam deflection procedure. On this basis, the closed-loop laser illumination (CLLI) for non-cooperative targets is realized, and the Cramer-Rao lower bounds (CRLB) performance in the lower limit of the pointing error is analyzed. Additionally, a Monte Carlo simulation system is built, and the performance of the CLLI algorithm is analyzed. The simulation results demonstrate that the triangular-scanning algorithm has good performance and can accurately estimate the direction of the boresight to achieve CLLI. Further study shows that the simulation results agree well with theoretical estimations and approximate the CRLB at the lower limit.

Piston sensing of sparse aperture systems with a single broadband image via deep learning.

The pistons of sparse aperture systems need to be controlled within a fraction of a wavelength for the system's optimal imaging performance. In this paper, we demonstrate that deep learning is capable of performing piston sensing with a single wide-band image after appropriate training. Taking the sensing issue as a fitting task, the deep learning-based method utilizes a deep convolutional neural network to learn complex input-output mapping relations between the broadband intensity distributions and corresponding piston values. Given a trained network and one broadband focal intensity image as the input, the piston can be obtained directly and the capture range achieving the coherence length of the broadband light is available. Simulations and experiments demonstrate the validity of the proposed method. Using only in-focused broadband images as the inputs without defocus division and wavelength dispersion, obviously relaxes the optics complexity. In view of the efficiency and superiority, it's expected that the method proposed in this paper may be widely applied in multi-aperture imaging.

Application of the Wiener filter for intensity noise reduction in fiber optic gyroscopes.

An essential issue for the low-noise system application of the fiber optic gyroscope (FOG) is to reduce its noise level. The relative intensity noise (RIN) of the light source is the dominant noise of the FOG when the light power on the detector reaches a certain level. The noise subtraction method is effective for RIN reduction and easy to implement in a FOG. This paper theoretically analyzes the factors that influence the result of the method and deduces the function to calculate the noise suppression ratio that can be achieved. A method that uses an optimum filter design based on the Wiener filter in the reference detector signal is proposed to improve the subtraction result. A FOG system is set up to test the feasibility of the method. The experiment results meet with the theoretical analysis, and by using the Wiener filter, the achieved noise subtraction factor reaches the limitation that restrains the optical system and detection circuit.

Electron acceptors for anaerobic oxidation of methane drive microbial community structure and diversity in mud volcanoes.

Mud volcanoes (MVs) emit globally significant quantities of methane into the atmosphere, however, methane cycling in such environments is not yet fully understood, as the roles of microbes and their associated biogeochemical processes have been largely overlooked. Here, we used data from high-throughput sequencing of microbial 16S rRNA gene amplicons from six MVs in the Junggar Basin in northwest China to quantify patterns of diversity and characterize the community structure of archaea and bacteria. We found anaerobic methanotrophs and diverse sulfate- and iron-reducing microbes in all of the samples, and the diversity of both archaeal and bacterial communities was strongly linked to the concentrations of sulfate, iron and nitrate, which could act as electron acceptors in anaerobic oxidation of methane (AOM). The impacts of sulfate/iron/nitrate on AOM in the MVs were verified by microcosm experiments. Further, two representative MVs were selected to explore the microbial interactions based on phylogenetic molecular ecological networks. The sites showed distinct network structures, key species and microbial interactions, with more complex and numerous linkages between methane-cycling microbes and their partners being observed in the iron/sulfate-rich MV. These findings suggest that electron acceptors are important factors driving the structure of microbial communities in these methane-rich environments.

Adaptive piston correction of sparse aperture systems with stochastic parallel gradient descent algorithm.

A phased sparse aperture system provides an economic solution to get high resolution images with less volume and weight. The crucial point of such systems is adaptive correction of piston, that is, a close-loop control aiming at stabilizing the optical path differences within a fraction of the wavelength. In this paper, we present an autonomous phasing approach using stochastic parallel gradient descent algorithm through optimization of image quality. The synthetic system can be phased by iteratively commanding piston actuators without any additional optics. Simulations are first performed to test the validity. Then experimental results based on a binocular telescope testbed are presented, showing that our proposed close-loop control of piston correction doesn't only work with both laser and white-light point sources, but also with an extended object.

Compact optical image amplifier pumped by a sub-nanosecond Nd:YAG microlaser.

A compact sub-nanosecond optical image amplifier is proposed and demonstrated. It is pumped by an actively Q-switched diode-pumped Nd:YAG microlaser in virtue of the optical parametric amplification (OPA) technique and quasi-phase-matching scheme. Experimental results show that the optical image gain reaches 15.4 dB under a pump intensity of 15.3 MW/cm2. The influence of parametric fluorescence noise on the amplified image is obvious above 10 dB gain. The signal-to-noise ratio degradation of the amplifier image characterized by the noise figures is in sheer contrast with theoretical expectations. It shows that the experimental noise figure value reaches 2.2 dB, larger than the theoretical result of 0.7 dB at 15 dB gain.

Research on high-resolution imaging technology based on light field manipulation for a lenslet-based plenoptic camera.

In this paper, an aberration correction method for an extended target is proposed to solve the problem of the lenslet-based plenoptic camera not imaging clearly under the influence of aberrations. We propose a light field manipulation method to improve performance of the light field imaging system. The principle of this method is that the sub-aperture images extracted from the raw light field image are offset when the light field imaging system is affected by aberrations, and the symmetrical arrangement of the sub-aperture image array is destroyed. By repairing the symmetrical arrangement of the sub-aperture image array, the influence of phase aberrations on the imaging system can be eliminated, and the resolution of the plenoptic camera can be improved. We use an image correlation algorithm to process the sub-aperture images of the plenoptic camera, calculate and compensate each sub-aperture image's displacement caused by aberrations, and restore the symmetrical arrangement of the sub-aperture image array; then, a corrected high-resolution refocused image can be generated. In particular, this method uses only the raw light field information obtained by the plenoptic camera in a single exposure, without adding other hardware devices. Furthermore, it takes the extended target itself as the reference image, so the ideal position need not be calibrated in advance. Also, the parallax information of the sub-aperture images is retained, and the method is simple and easy to use. Numerical simulation and experimental results show that the technology proposed in this paper can work well for high-resolution imaging of a plenoptic camera with phase aberrations. This method can be potentially applied to analyze lens aberration, media-induced image distortion such as water turbulence in underwater imaging, and atmospheric turbulence in remote imaging. It may have important application prospects in the fields of astronomical object detection, remote sensing, etc.

Active sparse aperture imaging using independent transmitter modulation with improved incoherent Fourier ptychographic algorithm.

Optical sparse aperture imaging shows great promise for the next generation of high resolution systems. In this paper, we propose and demonstrate an active sparse aperture imaging approach using independent transmitter modulation to digitally overcome phasing errors, correct aberrations, and further improve resolution. The reported imaging scheme consists of a general sparse aperture system and an active illumination unit, specifically an independent pattern projector. A series of raw images are captured with the projector scanned to illuminate the object. Based on the acquired data set, the improved incoherent Fourier ptychographic algorithm is utilized to reconstruct sparse aperture images with distortions removed and contrast enhanced. Furthermore, thanks to illumination pattern modulation, higher resolution beyond the diffraction limit of the synthetic aperture system is gained as a benefit. Good-quality and higher-resolution sparse aperture imagery obtained by employing our proposed technique in both simulation and experiment demonstrates the effectiveness. The reported approach may provide new insights to address the phasing and image restoration problems of sparse aperture systems in the transmitting path rather than only in the receiving path.

Improvement of pointing accuracy for Risley prisms by parameter identification.

Risley prisms appear to be a promising solution to high-accuracy pointing and tracking. To improve the pointing accuracy of achromatic Risley prisms, an appropriate mathematical model is established, and the forward and inverse solutions are proposed. Focusing on the sources of systematic errors, an optimization method based on a genetic algorithm is proposed to identify the parameters of the physical model, including wedge angles, refractive indexes, and installations. Finally, the experimental platform is established. The pointing accuracy and the size of the blind zone are tested to prove the validity of the method. Experimental results show that the proposed method is effective to reduce the influence of manufacturing, installation, and measurement errors. The optimized pointing accuracy has been improved significantly. Within the maximum deflection angle of 3°, the maximum pointing error is reduced from 33 arcsec to less than 1 arcsec. And the angular dynamic range is found to be greater than 43 dB, able to meet the needs of the majority of applications. In addition, the test of the blind zone shows that the optimized parameters are consistent with the actual system.

Error-Based Observer of a Charge Couple Device Tracking Loop for Fast Steering Mirror.

The charge couple device (CCD) tracking loop of a fast steering mirror (FSM) is usually used to stabilize line of sight (LOS). High closed-loop bandwidth facilitates good performance. However, low-rate sample and time delay of the CCD greatly limit the high control bandwidth. This paper proposes an error-based observer (EBO) to improve the low-frequency performance of the CCD tracking system. The basic idea is by combining LOS error from the CCD and the controller output to produce the high-gain observer, forwarding into the originally closed-loop control system. This proposed EBO can improve the system both in target tracking and disturbance suppression due to LOS error from the CCD's sensing of the two signals. From a practical engineering view, the closed-loop stability and robustness of the EBO system are investigated on the condition of gain margin and phase margin of the open-loop transfer function. Two simulations of CCD experiments are provided to verify the benefits of the proposed algorithm.

[Effects of Modified Ruji Recipe in Preventing Relapse and Metastasis of Breast Cancer Patients with Negative Hormone Receptor].

Objective To observe the effect of Ruji Recipe (RR) (treated by syndrome typing) in preventing the relapse and metastasis of invasive ductal breast cancer patients with negative hormone receptor (HR) after surgery and chemotherapy. Methods Using a prospective, cohort method, 136 pa- tients with stage I - III C HR negative invasive ductal breast cancer were equally assigned to the treat- ment group (treated by RR in syndrome typing way) and the control group (routine follow-ups). Disease free survival (DFS) , overall survival (OS) , relapse and metastasis were observed in the two groups. Re- sults All patients were followed-up for 15 to 57 months, with the median follow-up of 44 months. The median DFS and OS had not reached. The 1. 0, 1. 5, 2. -0, and 3. 0 years DFS were 94.1 % (64/68) , 86. 4 % (51/59), 81. 8% (45/55), and 72. 0% (36/50) in the treatment group. They were 77. 9% (53/68), 67.2% (45)67), 60. 6% (40)66), and 54. 5% (36/66) in the control group. Significant difference existed in 1. 0, 1. 5, and 2. 0 years DFS between the two groups (X² = 7.403, 6.426, 6.459; P =0. 012, 0.013, 0. 016). No statistical difference existed in 1. 0, 1. 5, 2. 0, and 3. 0 years OS between the two groups (P >0. 05). Among triple negative breast cancer (TNBC) patients, 1. 0, 1. 5, 2. 0, and 3. 0 years DFS were 97. 0% (32/33), 92. 9% (26/28), 92.6% (2527), and 84. 6% (22/26) in the treatment group, 81. 5% (2227), 66. 7% (1827), 61. 5% (16/26), and 57. 7% (15/26) in the control group. Of them, significant difference existed in 1. 5, 2. 0, and 3. 0 years DFS between the two groups (X² =5. 893, 7. 293, 4. 591 ; P = 0. 015, 0. 007, 0. 032). At the end of follow-ups, relapse and metastasis occurred in 15 patients, local recur- rence in 2 patients, single organ metastasis in 6 patients, and multiple organs metastasis in 7 patients of the treatment group. The relapse and metastasis occurred in 30 patients , local recurrence in 2 patients , single organ metastasis in 12 patients, and multiple organs metastasis in 16 patients of the treatment group. Conclusions RR ( by syndrome typing) could improve DFS and delay progression of invasive ductal breast cancer patients with negative HR in the first 2 years after surgery. It also had certain value for relapse and metastasis of TNBC patients within 2 years.

Effects of Lipopolysaccharide Core Sugar Deficiency on Colanic Acid Biosynthesis in Escherichia coli.

UNLABELLED: When 10 Escherichia coli mutant strains with defects in lipopolysaccharide (LPS) core biosynthesis were grown on agar medium at 30°C, four of them, the ΔwaaF, ΔwaaG, ΔwaaP, and ΔwaaB strains, formed mucoid colonies, while the other six, the ΔwaaU, ΔwaaR, ΔwaaO, ΔwaaC, ΔwaaQ, and ΔwaaY strains, did not. Using light microscopy with tannin mordant staining, the presence of exopolysaccharide around the cells of the mutants that formed mucoid colonies could be discerned. The ΔwaaF mutant produced the largest amounts of exopolysaccharide, regardless of whether it was grown on agar or in liquid medium. The exopolysaccharide was isolated from the liquid growth medium of ΔwaaF cells, hydrolyzed, and analyzed by high-performance liquid chromatography with an ion-exchange column, and the results indicated that the exopolysaccharide was consistent with colanic acid. When the key genes related to the biosynthesis of colanic acid, i.e., wza, wzb, wzc, and wcaA, were deleted in the ΔwaaF background, the exopolysaccharide could not be produced any more, further confirming that it was colanic acid. Colanic acid could not be produced in strains in which rcsA, rcsB, rcsD, or rcsF was deleted in the ΔwaaF background, but a reduced level of colanic acid production was detected when the rcsC gene was deleted, suggesting that a change of lipopolysaccharide structure in ΔwaaF cells might be sensed by the RcsCDB phosphorelay system, leading to the production of colanic acid. The results demonstrate that E. coli cells can activate colanic acid production through the RcsCDB phosphorelay system in response to a structural deficiency of lipopolysaccharide. IMPORTANCE: Lipopolysaccharide and colanic acid are important forms of exopolysaccharide for Escherichia coli cells. Their metabolism and biological significance have been investigated, but their interrelation with the cell stress response process is not understood. This study demonstrates, for the first time, that E. coli cells can activate colanic acid production through the RcsCDB phosphorelay system in response to a structural change of lipopolysaccharide, suggesting that bacterial cells can monitor the outer membrane integrity, which is essential for cell survival and damage repair.

MEMS Inertial Sensors-Based Multi-Loop Control Enhanced by Disturbance Observation and Compensation for Fast Steering Mirror System.

In this paper, an approach to improve the disturbance suppression performance of a fast steering mirror (FSM) tracking control system based on a charge-coupled device (CCD) and micro-electro-mechanical system (MEMS) inertial sensors is proposed. The disturbance observation and compensation (DOC) control method is recommended to enhance the classical multi-loop feedback control (MFC) for line-of-sight (LOS) stabilization in the FSM system. MEMS accelerometers and gyroscopes have been used in the FSM system tentatively to implement MFC instead of fiber-optic gyroscopes (FOG) because of its smaller, lighter, cheaper features and gradually improved performance. However, the stabilization performance of FSM is still suffering a large number of mechanical resonances and time delay induced by a low CCD sampling rate, which causes insufficient error attenuation when suffering uncertain disturbances. Thus, in order to make further improvements on the stabilization performance, a cascaded MFC enhanced by DOC method is proposed. The sensitivity of this method shows the significant improvement of the conventional MFC system. Simultaneously, the analysis of stabilization accuracy is also presented. A series of comparative experimental results demonstrate the disturbance suppression performance of the FSM control system based on the MEMS inertial sensors can be effectively improved by the proposed approach.

Deletion of the genes waaC, waaF, or waaG in Escherichia coli W3110 disables the flagella biosynthesis.

Flagella assembly was investigated in the Escherichia coli W3110 wild-type strain and ΔwaaF, ΔwaaC, and ΔwaaG mutant strains that only synthesize lipopolysaccharide with different lengths, using transmission electron microscopy and whole genome transcriptome profiling. Under the electron microscope, the flagella were observed on the cell surface of the W3110 strain but not the ΔwaaC, ΔwaaF, or ΔwaaG strains. Transcriptional profiling showed that 1382 genes in ΔwaaC, 526 genes in ΔwaaF, and 965genes in ΔwaaG were significantly regulated compared to the control W3110 strain. These genes were further analyzed by gene ontology and KEGG pathway. Although there were significant transcriptional differences among the ΔwaaC, ΔwaaF, and ΔwaaG strains, genes related to flagella assembly and bacterial chemotaxis (the linkage between the flagella and the environment) were significantly down-regulated in all three strains. The data demonstrated that flagella assembly in E. coli depends on the length of lipopolysaccharide.