Biomechanical and functional variation in rat sciatic nerve following cuff electrode implantation.

BACKGROUND: Nerve cuff electrodes are commonly and successfully used for stimulating peripheral nerves. On the other hand, they occasionally induce functional and morphological changes following chronic implantation, for reasons not always clear. We hypothesize that restriction of nerve mobility due to cuff implantation may alter nerve conduction. METHODS: We quantified acute changes in nerve-muscle electrophysiology, using electromyography, and nerve kinematics in anesthetized Sprague Dawley rat sciatic nerves during controlled hindlimb joint movement. We compared electrophysiological and biomechanical response in uncuffed nerves and those secured within a cuff electrode using analysis of variance (ANOVA) and regression analysis. RESULTS: Tethering resulting from cuff implantation resulted in altered nerve strain and a complex biomechanical environment during joint movement. Coincident with biomechanical changes, electromyography revealed significantly increased variability in the response of conduction latency and amplitude in cuffed, but not free, nerves following joint movement. CONCLUSION: Our findings emphasize the importance of the mechanical interface between peripheral nerves and their devices on neurophysiological performance. This work has implications for nerve device design, implantation, and prediction of long-term efficacy.

A critical review of flexible and porous SERS sensors for analytical chemistry at the point-of-sample.

For decades surface enhanced Raman spectroscopy (SERS) has been intensely investigated as a possible solution for performing analytical chemistry at the point of sample origin. Unfortunately, due to cost and usability constraints, conventional rigid SERS sensors and microfluidic SERS sensors have yet to make a significant impact outside of the realm of academics. However, the recently introduced flexible and porous paper-based SERS sensors are proving to be widely adaptable to realistic usage cases in the field. In contrast to rigid and microfluidic SERS sensors, paper SERS sensors feature (i) the potential for roll-to-roll manufacturing methods that enable low sensor cost, (ii) simple sample collection directly onto the sensor via swabbing or dipping, and (iii) equipment-free separations for sample cleanup. In this review we argue that movement to paper-based SERS sensors will finally enable point-of-sample analytical chemistry applications. In addition, we present and compare the numerous fabrication techniques for paper SERS sensors and we describe various sample collection and sample clean-up capabilities of paper SERS sensors, with a focus on how these features enable practical applications in the field. Finally, we present our expectations for the future, including emerging ideas inspired by paper SERS.

Inkjet-Printed Paper Fluidic Devices for Onsite Detection of Antibiotics Using Surface-Enhanced Raman Spectroscopy.

Surface enhanced Raman spectroscopy (SERS) provides rapid and sensitive identification of small molecule analytes. Traditionally, fabrication of SERS devices is an expensive process that involves the use of micro- and nano-fabrication procedures. Further, acquisition of diverse sample types requires complex preparation procedures that limits SERS to lab-based applications. Recent innovations using plasmonic nanoparticles embedded in flexible paper substrates has allowed the expansion of SERS techniques to portable analytical procedures. Recently inkjet-printing has been identified as a low cost, rapid, and highly customizable method for producing paper based SERS sensors with robust performance. This chapter details the materials and procedures by which inkjet printed SERS sensors can be fabricated and applied to relevant applications. In particular, methods for utilizing the sensors for detection of antibiotics are presented.

Vertical-flow paper SERS system for therapeutic drug monitoring of flucytosine in serum.

A number of life-saving drugs require therapeutic drug monitoring (TDM) for safe and effective use. Currently, however, TDM is performed using sophisticated analytical techniques relegated to central labs, increasing the cost per test and time to answer. Here, using a novel vertical flow membrane system with inkjet-printed surface enhanced Raman sensors, along with a portable spectrometer, we demonstrate a low cost and easy to use device to quantify levels of flucytosine, an antifungal that requires TDM for effective patient care, from undiluted human serum. To our knowledge, this work represents the first report of a passive vertical flow sample cleanup method with surface enhanced Raman detection. We first investigated and optimized the parameters of the vertical flow system for the detection of flucytosine in spiked serum samples. Then, using an optimized vertical-flow system utilizing nitrocellulose membranes and a paper SERS sensor, we achieved detection of down to 10 µg mL-1 flucytosine in undiluted serum, with quantitative detection across the entire therapeutic range. This system reduces the assay time to about 15 min, far quicker than the current gold standards. We anticipate that this novel system will enable near-patient therapeutic drug monitoring, leading to the safe and effective administration of a number of life-saving drugs. Furthermore, it will spawn the development of SERS detection systems capable of separating target analytes from real-world biological matrices.